References

Biogeosciences

1726-4189

Copernicus Publications

Göttingen, Germany

10.5194/bg-10-3679-2013

Response of bacterioplankton community structure to an artificial gradient of pCO2 in the Arctic Ocean

Zhang

¹ ² ⁵ Xia

¹ ² ⁵ Lau

S. C. K.

³ Motegi

⁴ Weinbauer

M. G.

⁴ Jiao

¹ ²

State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China

Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen, 361005, China

Division of Life Science and Division of Environment, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China

Microbial Ecology and Biogeochemistry Group, Université Pierre et Marie Curie-Paris 6, Laboratoire d'Océanographie de Villefranche, 06230 Villefranche-sur-Mer, France; CNRS, Laboratoire d'Océanographie de Villefranche, 06230 Villefranche-sur-Mer, France

These authors contributed equally to this work.

04 06 2013

10 6 3679 3689

2013

This work is licensed under the Creative Commons Attribution 3.0 Unported License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/3.0/

This article is available from https://bg.copernicus.org/articles/10/3679/2013/bg-10-3679-2013.html

The full text article is available as a PDF file from https://bg.copernicus.org/articles/10/3679/2013/bg-10-3679-2013.pdf

In order to test the influences of ocean acidification on the ocean pelagic ecosystem, so far the largest CO2 manipulation mesocosm study (European Project on Ocean Acidification, EPOCA) was performed in Kings Bay (Kongsfjorden), Spitsbergen. During a 30 day incubation, bacterial diversity was investigated using DNA fingerprinting and clone library analysis of bacterioplankton samples. Terminal restriction fragment length polymorphism (T-RFLP) analysis of the PCR amplicons of the 16S rRNA genes revealed that general bacterial diversity, taxonomic richness and community structure were influenced by the variation of productivity during the time of incubation, but not the degree of ocean acidification. A BIOENV analysis suggested a complex control of bacterial community structure by various biological and chemical environmental parameters. The maximum apparent diversity of bacterioplankton (i.e., the number of T-RFs) in high and low pCO2 treatments differed significantly. A negative relationship between the relative abundance of Bacteroidetes and pCO2 levels was observed for samples at the end of the experiment by the combination of T-RFLP and clone library analysis. Our study suggests that ocean acidification affects the development of bacterial assemblages and potentially impacts the ecological function of the bacterioplankton in the marine ecosystem.

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