Filtration artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays

Inland waters are large contributors to global carbon dioxide (CO2) emissions, in part due to the vulnerability of dissolved organic matter (DOM) to microbial decomposition and respiration to CO2 during transport through aquatic systems. To assess the degree of this vulnerability, aquatic DOM is often incubated in standardized biolability assays. These assays isolate the dissolved fraction of aquatic OM by size filtration prior to incubation. We test whether this size selection has an impact on the bacterial community composition and the consequent dynamics of DOM degradation using three different filtration strategies: 0.2 μm (filtered and inoculated), 0.7 μm (generally the most common DOM filter size) and 106 μm (unfiltered). We found that bacterial community composition, based on 16S rRNA amplicon sequencing, was significantly affected by the different filter sizes. At the same time, the filtration strategy also affected the DOM degradation dynamics, including the δ13C signature. However, the dynamics of these two responses were decoupled, suggesting that filtration primarily influences biolability assays through bacterial abundance and the presence of their associated predators. By the end of the 41-day incubations all treatments tended to converge on a common total DOM biolability level, with the 0.7 μm filtered incubations reaching this point the quickest. These results suggest that assays used to assess the total biolability of aquatic DOM should last long enough to remove filtration artefacts in the microbial population. Filtration strategy should also be taken into account when comparing results across biolability assays.


Introduction
Research showing that inland waters are significant sources of carbon dioxide (CO 2 ) to the atmosphere (Cole et al., 2007) has led to a large increase in the number of studies that consider the magnitude and source of this CO 2 .Inland waters are estimated to release CO 2 equivalent to ∼ 19 % of global anthropogenic CO 2 emissions annually (Le Quéré et al., 2016;Raymond et al., 2013).One potentially important source of this CO 2 is dissolved organic matter (DOM), which is present in relatively high concentrations in many inland water systems (e.g.Evans et al., 2014;Dean et al., 2016).The contribution of CO 2 from microbial respiration in aquatic systems is an important component of understanding the global carbon cycle (e.g.McCallister and del Giorgio, 2012), as well as ecosystem dynamics in this important interface between the terrestrial and marine realms (Aufdenkampe et al., 2011;Guillemette et al., 2017).Biolability assays, which determine the vulnerability of DOM to microbial decomposition during aquatic transport are increasingly common for determining the magnitude and importance of aquatic DOM as a CO 2 source (Guillemette and del Giorgio, 2011;Vonk et al., 2015).
Standardized biolability assays allow for the comparison of DOM vulnerability to decomposition during aquatic transport across a range of systems (Findlay and Sinsabaugh, 2003;Guillemette and del Giorgio, 2011;Vonk et al., 2015).These approaches involve isolation of the dissolved fraction of organic matter (DOM) over short-(< 2 days) and long-term (∼ 28 days) incubations depending on the research question (Guillemette and del Giorgio, 2011).For determin-ing the total biolability of an aquatic DOM sample, long-term assays tend to be more common (e.g.Spencer et al., 2015).
The DOM size class has variously been defined as the fraction of organic matter molecules smaller than ∼ 1 µm (e.g.del Giorgio and Pace, 2008), 0.7 µm (e.g.Mann et al., 2015), 0.45 µm (e.g.Drake et al., 2015) and 0.2 µm (e.g.Logue et al., 2016).The lowest size cut-off, 0.2 µm, is often assumed to be biologically sterile as no microbes are thought to be smaller than this (Gasol and Morán, 1999), although tests have shown that viable microbial communities can develop even in < 0.2 µm filtrate (Hahn, 2004).Filtration to 0.7 µm, arguably the most common size cut-off (the pore size of standard glass fibre filters [GF/F] used in water filtration applications) would also likely exclude the majority of microbes (Ferguson et al., 1984;Gasol and Morán, 1999).Biolability assays that filter to 0.2 µm commonly include an inoculation of the incubations after filtration with in situ (or study relevant) unfiltered microbial communities to reduce community effects (e.g.Logue et al., 2016).However, biolability assays filtered to 0.45 and 0.7 µm are often not inoculated and it is assumed that enough bacteria will pass through the filter to decompose and respire the filtrate DOM (Vonk et al., 2015).In biolability studies, specifically in permafrost regions, the effect of filter size was shown to be insignificant (Vonk et al., 2015).However, due to the extreme size filtration this is a surprising result given that these filter sizes will exclude a large proportion of microbes, potentially causing a significant shift in the microbial community structure and therefore potentially altering the dynamics of the decomposition processes mediated by the microorganisms (Logue et al., 2016;Traving et al., 2016).
We aimed to test the significance of this potential microbial shift resulting from filtration by answering the following questions: (1) how do different filtration strategies affect the bacterial community composition in DOM incubations?(2) Does this influence the outcome of DOM biolability assays?We tested these questions using an experimental set-up with three filtration treatments applied during biolability assays of organic carbon-rich water draining a temperate peatland in the Netherlands.

Field sampling
The organic carbon-rich water for the DOM incubations was collected from a ditch draining Horstermeer peatland (52.144 • N, 5.043 • E) (Hendriks et al., 2007).The site is former agricultural land, abandoned in the mid-1990s, in a drained natural lake in the central Netherlands.The underlying geology is Pleistocene aeolian sands, overlain by peat and organic-rich lake deposits (Hendriks et al., 2007).Approximately 20 L of water was collected in spring 2016 (16 March), pre-filtered to 106 µm and transported to Vrije Universiteit Amsterdam in two acid-washed plastic vessels.The samples were not refrigerated during transport, but travel time was less than 1 h and the samples were refrigerated at 4 • C immediately upon arrival at the university until filtration for the incubations on the same day.

Incubations
The sample water was prepared for incubation with three filtration strategies: (1) 3 L of sample water was filtered to 0.2 µm (sterilized nylon membrane, Whatman), homogenized and inoculated with 5 mL of sample water filtered to 106 µm (henceforth P2 treatment).( 2) 3 L of sample water filtered to 0.7 µm (pre-ashed glass fibre filter, Whatman; GF/F) and homogenized (henceforth P7 treatment).(3) 3 L of sample water was homogenized but not filtered further than 106 µm (henceforth UF -unfiltered).Subsamples for dissolved organic carbon (DOC -the carbon component of the DOM pool) concentrations and quality analyses (CDOM) were collected from the homogenized treatments.
For each treatment, 100 mL of sample water was decanted into 25 individual 125 mL acid-washed HDPE bottles for incubation.The bottles were incubated at 20 • C in completely dark conditions following standard protocol (Vonk et al., 2015).Bottle lids were loosely placed on top to ensure there was sufficient oxygen for bacterial respiration to occur.Five bottles for each treatment were removed from the incubation chamber on days 5, 14, 27 and 41 to enable sampling and analysis of DOM and microbial community dynamics during incubation.All incubation bottles were gently shaken every 2 to 3 days to limit flocculation and settling of organic matter at the bottom of the incubation bottles.
Five individual 125 mL acid-washed HDPE bottles were filled with 100 mL demineralized water and incubated under the same conditions as the treatments to serve as blanks.Carbon concentrations in the blanks increased from 0.01 ± 0.02 to 0.05 ± 0.02 mmol L −1 over 41 days, representing an increase of 0.03 mmol L −1 , likely because the demineralized water was not completely sterile.These values represent ∼ 1 % of the initial DOC concentrations and a maximum of ∼ 15 % of the lowest final DOC concentration, suggesting that any possible carbon input from the plastic bottles was small relative to the DOM dynamics observed during the incubation.Incubation samples would not have been affected by the suspected biological growth in the non-sterile demineralized blanks because the incubation bottles only contained sample water and were not treated with the demineralized water in any way.

Sample collection and analysis
DOC concentration subsamples were collected by re-filtering to isolate the dissolved component (Vonk et al., 2015).For the P2 treatment, these subsamples were filtered to 0.2 µm (regenerated cellulose membrane, Whatman) using sterile syringes; for the P7 and UF treatments, they were filtered to 0.7 µm (pre-ashed GF/F, Whatman) -this was to ensure the same size class of DOM was compared for each treatment.Samples were frozen immediately after collection and analysed within 6 months, which should not have had any significant impact on the concentration or isotope composition of the DOC analyses (Gulliver et al., 2010;Peacock et al., 2015).The most commonly used filter sizes for DOM incubations are 0.2 and 0.7 µm (Vonk et al., 2015).A filter size of 0.45 µm is also common for a DOC sample collection, but less so for incubations.DOC concentrations and δ 13 C-DOC isotopes were measured on a high-temperature combustion total organic carbon system (varioTOC cube; Elementar Analysensysteme GmbH) coupled to an isotope ratio mass spectrometer (IsoPrime 100; Isoprime Ltd, UK), using an independent calibration curve (measurement range 0.02 to 12.5 mmol L −1 ) with 13 C isotope standards IAEA-600, caffeine and IAEA-CH6, sucrose (Federherr et al., 2014;Kirkels et al., 2014).
DOM absorbance measurements (CDOM) were carried out immediately after sample filtration using a double-beam spectrophotometer (Shimadzu UV-1601PC).Absorbance spectra were determined in matched 1 cm quartz cuvettes over 200-750 nm, at 0.2 nm intervals, using fresh demineralized water as a reference.We calculated standard CDOM indices, SUVA 254 , S R , E2 : E4 and E4 : E6 ratios, and also present the specific absorption (a) at 240, 300 and 440 nm (Helms et al., 2008).Measured absorbance values were converted to absorption coefficients: where A(λ) is the absorbance at wavelength λ (nm) and l is cuvette path length (m).The slope coefficient, S CDOM in nm −1 , is defined in the following equation (Helms et al., 2008): where a CDOM (λ r ) is the CDOM absorption at a reference wavelength λ r , and K is the parameter that offsets baseline shifts unrelated to CDOM absorption.S CDOM was fitted for each sample over the wavelength ranges of 275-295 and 350-400 nm using linear regression of the log-transformed absorption coefficients.The ratio of these spectral slopes (S R ) has been shown to provide insights into changes in DOM molecular weight (Helms et al., 2008).

DNA extraction and amplicon sequencing
At each sampling occasion, 20 mL of each sample was filtered to 0.2 µm (regenerated cellulose membrane, Whatman) using sterile syringes.These filters were subjected to DNA isolation for subsequent 16S amplicon community profiling.DNA was extracted from filters using the MoBio Powersoil DNA isolation kit (MoBio, Carlsbad, USA) following the standard protocol.Successful DNA extraction was confirmed by visualization on agarose gel and quantification with Nan-oDrop (ThermoFisher, USA) in comparison to procedural blanks (sterile filters).Amplicons for sequencing were generated by a two-step polymerase chain reaction (PCR).The first PCR was with universal bacterial and archaeal primers targeting the V3-V4 region of the 16S rRNA gene (341F and 806R).Reaction mixtures were 12.5 µL Phusion High-Fidelity Master Mix (ThermoFisher Scientific, USA), 1 µL of each primer at 10 nM concentration, 9 µL of nucleasefree water and 1.5 µL of DNA sample.The PCR programme was an initial step at 98

Bioinformatics
Our amplicon sequencing procedure produced 4.9 million pairs of raw reads.The original intention was to assemble contigs from the paired-end reads, but low read quality in the tail (last 150 cycles) made merging of paired-end reads impossible.We therefore analysed single reads trimmed to 180 and 150 bases for forward and reverse reads respectively.We performed analysis on both forward (V3 region) and reverse (V4 region) reads in parallel using a common bioinformatics pipeline.Raw reads were processed by trimming primers and applying quality filtering with the recommended maximum expected error rate of 1 (Edgar and Flyvbjerg, 2015).Unique reads were subsequently sorted by abundance, singletons discarded and operational taxonomic units (OTUs) defined using the UPARSE-OTU algorithm (Edgar, 2013) with minimum similarity set to 97 %.The original set of truncated and quality-filtered reads were mapped back to the resulting OTUs to create an OTU table of read abundance per OTU per sample.Representative sequences for each OTU were aligned using the tool PyNAST (Caporaso et al., 2010a) and assigned to taxonomy using the RDP classifier (Wang et al., 2007) using QIIME version 1.7.0 scripts (Caporaso et al., 2010b) and Greengenes 2011 release as the reference database (DeSantis et al., 2006).Candidate OTUs that failed to align with more than 70 % similarity were discarded as www.biogeosciences.net/15/7141/2018/Biogeosciences, 15, 7141-7154, 2018 putative artefacts.All remaining OTUs were assembled into a phylogenetic tree using the FastTree algorithm (Price et al., 2009).Subsequent statistical analyses were performed on OTU tables rarefied to 8000 sequences per sample.

Statistics
The DOC concentration measurements exhibited complex temporal dynamics that differed between filtration treatment making it impractical to fit a single parametric model, so we analysed the data using generalized additive models (GAMs) (Wood, 2006;Catalán et al., 2017).We fit a series of models, in every case modelling DOC concentration as a smooth function of time, but by either fitting (1) a single smoother for all treatments combined, (2) a separate smoother for each treatment or (3) a single smoother for P2 and UF and a separate one for P7.For each model we also tested the effect of the choice of smoothing parameter (k = 3 or 4, related to the degree of flexibility permitted in the curve fitting).Model selection using Akaike information criterion (AIC) (Johnson and Omland, 2004) was employed to identify the best-fitting model from these six candidate models.
For each of the seven DOM quality indices, we analysed the effects of incubation time and treatment, and their interaction, using a two-way ANOVA model.Since each observation was from an independent incubation flask, no adjustments for repeated measurements were made.Time was treated as a discrete factor.
Relationships between bacterial community profiles were visualized by Non-metric multidimensional scaling (NMDS) ordination of Unifrac distance matrices computed from the rarefied OTU table and associated de novo tree of OTU representative sequences.Unifrac is a pairwise phylogenetic similarity measure defined as the proportion of tree length shared by OTUs detected in any two samples (Lozupone and Knight, 2005).It therefore expresses the degree of similarity between samples, weighted by the phylogenetic distinctness of the differentiating taxa.We performed ordinations based on both the unweighted (taking into account only presence or absence of taxa) and weighted (also weighing taxa by their abundances in the OTU table) versions of Unifrac, as these provide complementary explorations of the relationships between community profiles (Lozupone and Knight, 2005).A more formal analysis of the difference in community profiles between treatments and sampling occasions was performed using permutational multiple analysis of variance (PERMANOVA) (Anderson, 2001;Oksanen et al., 2011), using both the weighted and unweighted Unifrac matrices as the response variable, and filtration treatment and sampling time as fully crossed fixed factors.All statistical analyses were performed using the R software package (R Core Team, version 3.4.4).

DOC dynamics
DOC concentrations in sample water measured directly after field collection averaged 1.09 mmol L −1 (standard error [SE] = 0.01, n = 10; Fig. 1).Over the course of the incubation, there was a decrease in DOC concentrations with different dynamics between filtration strategies.P7 showed roughly negative exponential decay dynamics from the beginning (Fig. 1) and reached a final DOC concentration after 41 days of 0.225 mmol L −1 (SE = 0.001, n = 5), equivalent to 21 % of initial concentrations.Both UF and P2 showed temporal dynamics with a clear lag phase: large decreases in DOC concentrations were not apparent after 28 days, but after 41 days they approached convergence with P7, with final values at 49 % and 31 % of initial concentrations, respectively (Fig. 1).It is generally more common to fit a negative exponential model to DOM biolability data.However, this function is strictly constrained to decreasing trends and cannot be sensibly fit to data that show increases, as seen in this study (Fig. 1).We attempted to fit negative exponential models to the same data, but this resulted in a much poorer fit (AIC = −13; Fig. S1 in the Supplement).The difference in dynamics according to the filtration strategy was statistically significant when analysed with GAMs, since the best-fitting model included separate smoothers for each filtration treatment (adjusted R 2 = 0.89, estimated d.f.= 11.0,Table 1).Two points at UF day 41 could be considered outliers; however, removing them from the data set leads to qualitatively the same conclusions with regards to the best-fitting model by the AIC model comparison and the following predicted values on day 27 (proportion DOC remaining, mean ± standard error, n = 5 in all cases; P2 = 98 % ± 3, P7 = 27 % ± 3, UF = 86 % ± 3), and on day 41 (P2 = 31 % ± 3, P7 = 21 % ± 3, UF = 24 % ± 4) (Table 1; Fig. 1).
The δ 13 C-DOC values remained stable during incubation, within ±2.0 ‰ (Fig. 2), although this variability is slightly more pronounced than in other dark bacterial decomposition experiments of aquatic DOC (±0.0-0.5 ‰; Lalonde et al., 2014;Vähätalo and Wetzel, 2008).The shifts in δ 13 C-DOC could be due to the preferential mineralization of certain DOC molecules with distinct δ 13 C signatures: a more positive δ 13 C signature for all samples on day 5 and a more negative δ 13 C signature in only the P7 samples on day 14.The former shift reflects the initial increase in DOC concentrations seen in the P2 and P7 treatments, and the latter shift reflects the change in DOC degradation dynamics seen only in the P7 treatment.As the experiment progressed, the δ 13 C across all samples converge, although over a wide range (−28.0 ‰ to −29.0 ‰; Fig. 2).DOC concentrations in P2 and P7 were slightly higher than initial measurements on day 5 (Fig. 1), suggesting there may have been growth of chemoautotrophic organisms (photo-autotrophy can be ruled out given the dark Table 1.Analysis of temporal DOC concentration dynamics.Three candidate GAM models were fit including a non-linear smoothing term s.These models correspond to the following scenarios: (a) treatments differ in initial concentration but show identical temporal dynamics; (b) treatments differ in initial concentration but with unique temporal dynamics of DOC concentration for each treatment; (c) treatments differ in initial concentration but with separate dynamics for P7 relative to P2 and UF.For each model two possible fitting parameters (k) were tested.Higher values of k allow more flexible model forms.Values reported are AIC with the percentage of deviance explained by the model in parentheses, followed by the estimated degrees of freedom.AIC is a measure of relative goodness of fit for each model to the observed data; lower AIC values indicate better fit.The best fit model is printed in bold and was used to generate the non-linear regression lines in Fig. 1.  1).incubations).A likely candidate is nitrification, given the relatively large concentrations of ammonia in the water column (29-82 µmol L −1 NO 3 + NO 2 , Ko van Huissteden, personal communication, 2017).This is supported by rapid growth in microbial cell counts over the same period (Logue et al., 2016) (Fig. S2 in the Supplement).However, the shifts in δ 13 C-DOC do not provide any clear line of reasoning for these differing DOC dynamics (for example a shift towards less negative δ 13 C-DOC values due to chemoautotrophic fixation of ambient CO 2 on day 5).
DOM quality showed a consistent pattern across filtration treatments.All the absorption parameters indicate a rapid shift from more aromatic and higher molecular weight DOM to much more degraded and smaller molecules by day 5 (Fig. 3).This is more pronounced in SUVA 254 , E2 : E3 ratios and absorptions at 240, 300 and 440 nm compared to S R , the latter of which shows greater scatter, but the same general trend.Moreover, factorial ANOVA analyses show that the temporal effect was always stronger than any effect of filtration treatment (Table 2).The E4 : E6 ratios did not change significantly over the course of the experiment, nor did they differ between filtration strategy treatments.This shows that the DOC concentration dynamics are decoupled from DOM structural dynamics, indicating that microbial activity is playing an important role in DOC concentrations, but that overall molecular degradation was consistent across the treatments.
Based on the lack of a significant difference between DOC concentrations in the treatments at the initial time point (Fig. 1), there is very little DOC contained between the 0.2 and 0.7 µm size range; this is supported by previous work (Zsolnay, 2003;Bouillon et al., 2014;Denis et al., 2017).Most DOM molecules are very small, less than ∼ 0.1 µm in size (Gustafsson and Gschwend, 1997), with very little in the 0.2 to 0.7 µm size range contributing to the overall DOC concentration.This is also reflected in the DOM quality indices in this study, although the P2 treatment shows some differences initially (Fig. 3).The lower E2 : E3 ratio and higher S R initial values for P2 compared to P7 suggests that the P2 samples are of lower molecular weight in general, while the higher SUVA254 values suggest greater aromaticity in the P2 samples (Helms et al., 2008).This difference is most clearly seen in the absorbance at 440 nm (Fig. 3e).However, these differences appear relatively minor in magnitude, with the www.biogeosciences.net/15/7141/2018/Biogeosciences, 15, 7141-7154, 2018  structural indices converging by the first time point (day 5) across all treatments during incubation (Fig. 3).This suggests that what small initial structural differences existed between the P2 and P7 treatments appeared unimportant to the overall dynamics of the DOM pool, which is supported by the lack of difference in initial DOC concentrations (Fig. 1) and previous work (Zsolnay, 2003;Bouillon et al., 2014;Denis et al., 2017).

Microbial community structure
Bacterial community analyses based on the V3 and V4 regions were qualitatively very similar.We here present results based on the longer and higher-quality V3 sequences.This interpretation is supported by the results of the PER-MANOVA, which show that for the abundance-weighted analysis both incubation time, treatment and their interaction significantly contribute to variation in the community distance matrix (all permutation P < 0.0001, proportion of variance explained: time = 66 %, filtration treatment = 8 %, time × filtration = 15 %).For presence-absence-based analysis, treatment and time effects were still significant but the latter was relatively weaker (all permutation P < 0.0001, proportion of variance explained: time = 29 %, filtration treatment = 12 %, time × filtration = 14 %).The patterns described above are a result of complex temporal dynamics in the relative abundances of different bacterial taxa (Figs.S3 and S4 in the Supplement).Focusing on the most abundant taxa, across all treatments the relative abundances of reads assignable to Alphaproteobacteria, Bacteroidetes, Verrucomicrobia, Planctomycetes and Actinobacteria all tended to increase over the course of the incubation.Conversely, reads assignable to the candidate phylum OD1 and Parvarchaeota, and unassignable reads, all showed negative trends in relative abundance over the course of the incubation.Reads assignable to the classes Beta-, Gamma-, Epsilonand Gammaproteobacteria showed more variable dynamics exhibiting large changes in relative abundance in the first 5 days, followed by stabilization for the remainder of the incubation.When comparing treatments, the major differences were due to the timing and magnitude of shifts in these general patterns.More specifically, Actinobacteria showed a much stronger increase in relative abundance over the incubation in UF samples relative to the other treatments; Bacteroidetes started lower and peaked later in P7 relative to the other two treatments; Betaproteobacteria had a less pronounced peak in UF relative to the other two treatments; UF samples showed relatively lower Alphaproteobacteria and higher Deltaproteobacteria relative abundances in the latter part of the incubation; Verrucomicrobia reached a higher abundance in P2 samples relative to the other two treatments; for OD1 and Parvarchaeota, P7 had higher initial relative  2).abundances than the other two treatments, but for both taxa the abundances from 14 days onwards were broadly similar across treatments (Figs. 4,S3 and S4).

Discussion
Filtration prior to incubation is standard practice in DOM biolability assays.Our results show that the specific choice of filtration strategy can have persistent consequences for the community composition of bacterioplankton throughout the course of the incubation, as well as on the dynamics of DOM degradation.

How does filter treatment affect bacterial community composition?
The community profile data show consistent succession in bacterial community composition over the 41 days of incubation (Fig. 4).Similar temporal patterns of succession over short timescales are regularly observed in bottle incubations (e.g.Massana et al., 2001;Baltar et al., 2012) and are also characteristic of seasonal dynamics in bacterioplankton communities in situ (Gilbert et al., 2009;Rösel et al., 2012).These changes most likely reflect dynamics driven by differwww.biogeosciences.net/15/7141/2018/Biogeosciences, 15, 7141-7154, 2018 ent growth rates, resource competition and, potentially, predation.In our study system, UF samples (unfiltered) and P2 samples (filtered to 0.2 µm and inoculated with unfiltered sample) had similar bacterioplankton community compositions at the beginning of the incubation.During the course of the incubation, however, the bacterioplankton community compositions of P2 and P7 (filtered to 0.7 µm but not inoculated) treatments became more similar, a pattern that persisted until the end of the incubation.When assessed with a presenceabsence-based distance metric, the different treatments did not converge (Fig. 4a), showing that for the timescales relevant for incubation, filtration strategy significantly alters bacterioplankton community composition.
Filtration strategy is likely to have affected the bacterial community composition in our incubations through three different mechanisms.
Firstly, the direct size selection effect of the different filter treatments is likely to be enriched for bacterial taxa that can pass through a given filter size (Gasol and Morán, 1999), thereby determining the community structure at the beginning of the incubation.In our study, samples filtered to 0.7 µm were particularly enriched in candidate phylum OD1 and Parvarchaeota, but depleted in Bacteroidetes, Epsilon-, Deltaand Betaproteobacteria at the very beginning of the incubation relative to P2 and UF samples.OD1 is a poorly characterized taxon, although some evidence points to its members being particularly abundant in the hypolimnion of lakes (Peura et al., 2012).Parvarchaeota are considered amongst the smallest microorganisms currently known (Chen et al., 2018), so their higher relative abundance is not surprising given they would readily fit through the 0.7 µm filter pore.The various Proteobacteria classes encompass a wide range of ecological niches in freshwater environments (Newton et al., 2011).This large variety makes it difficult to relate the observed patterns in our study to specific functions, but the depletion of Betaproteobacteria at the beginning of the incubation in P7 samples agrees with the observation that members of this group tend to be fast-growing and therefore tend to have larger cell sizes (Newton et al., 2011).Of the other two treatments, it is interesting to note that the P2 samples more closely resembled the UF samples in the early stages of the incubation.This implies that, initially at least, the bacterial community in P2 was dominated by taxa introduced with the inoculum, despite the fact that members of some freshwater bacterial phyla can pass a 0.2 µm filter (Hahn, 2004).This is evidence for the efficacy of inoculation treatments in biolability studies.
A second mechanism by which filtration could affect bacterial community composition is through the activity of bacterial grazers.Given the important role of bacterial predation by ciliates, flagellates and other microeukaryotes in structuring bacterioplankton communities (Hahn and Hofle, 2001), it is logical to expect that any filtration strategy that reduces predator abundance would influence the development of the bacterioplankton community in an incubation (although we did not characterize the bacterivorous community in this study).For example, if UF samples contained a bacteriovore community closest to that found in the source system, then the higher relative abundances of Actinobacteria and lower peak of Betaproteobacteria could result from grazingmediated selection; Actinobacteria are generally considered defense specialists, while Betaproteobacteria have been observed to be sensitive to grazing pressure (Hahn and Hofle, 2001;Newton et al., 2011).
A third possible mechanism by which filtration could influence bacterial community composition is the effect of filtration on the quantity and chemical composition of the DOM pool.If DOM chemical composition is related to its size fraction, then filtration could potentially change the quality and structure of the substrate available to heterotrophs leading to divergences in community composition due to differential specialization on carbon substrates (Logue et al., 2016).The different filtration strategies used in this study did not significantly affect the overall structural characteristics of the DOM pool (Table 2; Fig. 3), so this is unlikely to have influenced the bacterial community composition or its evolution through time.Further, the shift in the bacterial community was observed to occur over longer timescales than any shift seen in the DOM structural properties, which all reached equilibrium by day 14 (Table 2; Fig. 3).

Does the change in bacterial community
composition affect DOM biolability assays?
The DOM dynamics of P7 were distinct from P2 and UF, which in general behaved the same through the course of the incubation (Fig. 1).This is the opposite to what we see in the bacterial community composition dynamics in which UF was distinct from P7 and P2 (Fig. 4).DOM degradation dynamics may therefore be driven by the bacterial community composition commonalities in P2 and UF, and the bacterial community in P7 (the most common treatment/experimental design for DOM biolability studies) (Vonk et al., 2015) is more efficient at degrading DOM.However, in this study there was no evidence for the presence of high-level taxa that clearly associate with variations in DOM dynamics.This is most likely due to the high diversity of ecological traits within the higher-level taxonomic groupings we analysed (Martiny et al., 2015).All the major groups observed, especially Actinobacteria, Bacteriodetes and Proteobacteria, are known to be associated with the degradation of DOM in freshwater systems (Bauer et al., 2006;Gattuso, 2002;Newton et al., 2011).The lack of clear connection between these taxa and observed DOM degradation leads to the conclusion that degradation was not driven by specific changes in the bacterial community composition.We propose that the following two mechanisms could explain the observed dynamics: 1. Bacteriovores (grazers) limit the growth rate of bacterial communities (Baumgartner et al., 2016;Hahn and Hofle, 2001).This could explain the difference between P7 and UF, as the 0.7 µm filter pore size would likely exclude bacteriovores, which tend to be much larger than the bacteria they prey upon (Berdjeb et al., 2011).However, bacteriovores are likely to be present in the UF and P2 treatments (in the latter case due to inoculation).
2. Filtration will severely limit the number of cells that pass through specific filter pore sizes (Wang et al., 2008).In P2, DOM degradation rates may have been limited by the low initial population size caused by the size cut-off of the 0.2 µm filter, which would exclude the vast majority of bacteria (Hahn, 2004).The number of bacterial cells in the P2 treatments were consistently 31 %-75 % lower than in the UF treatments (Fig. S2 in the Supplement).
In this system, there is no evidence that filtration effects on DOM structure can explain the patterns in DOM degradation, nor (as discussed above) the bacterial community dynamics.We argue that filtration will instead influence the DOM dynamics mainly by a combination of influences on bacterial www.biogeosciences.net/15/7141/2018/Biogeosciences, 15, 7141-7154, 2018 community composition, bacterial abundance and the presence of their associated predators.

Wider implications, limitations and future work
Using a universal bacterial inoculum could prevent the biolability comparison issues raised by this study (Pastor et al., 2018).However, such a universal inoculum could also suffer from changes in microbial community composition over time depending on the DOM source and available nutrients, and/or be affected by microbes that slip through a 0.2 µm filter (Hahn, 2004).Further trials of universal inoculums across a range of aquatic environments would be beneficial in assessing these potential issues.This study suggests that filter size has relatively little impact on DOC concentration and DOM structure, supporting previous studies (Zsolnay, 2003;Bouillon et al., 2014;Denis et al., 2017).The choice of filter size for DOM sample storage, therefore, is relatively unimportant.What is more important for sample storage, and the subsequent degradation of the DOM pool by latent microbes, is the immediate treatment and storage conditions.For example, acidification, storage in dark conditions, refrigeration and freezing can all reduce microbial activity and maintain the integrity of DOM samples, but the best method is to analyse the samples as soon as possible after collection (Gulliver et al., 2010;Peacock et al., 2015).
This study is from a single peatland site in the Netherlands and therefore may not be representative of DOM biolability dynamics across a wider range of freshwater ecosystems.The organic carbon-rich nature of the study site and its water are consistent with temperate peatland characteristics elsewhere in the Northern Hemisphere (e.g.Billett et al., 2010), but the agricultural history of the site means that nutrient availability and the general chemistry of the sample waters are likely different from more pristine peatland systems.Such environmental factors are important influences on microbial communities (Dean et al., 2018;Gulis and Suberkropp, 2003), which would have influenced the development of bacterial community composition during the experiment presented here.
It is important to note that the microbial community patterns described here are based on relative abundances; thus, apparent declines in particular taxa may be attributable to either real declines in population or (as is more likely) relatively slower growth relative to other taxa in the community.Moreover, PCR amplicon generation is known to introduce biases in the community profile (e.g.Sipos et al., 2007), so any interpretation of these data for particular taxa should be further confirmed by more reliable quantitative methods such as taxon-specific qPCR (Smith and Osborn, 2009), FISH (Amann et al., 1995) or PCR-free metagenomics methods (Handelsman, 2004).
Despite the lack of clear connection between bacterial community composition and DOM dynamics, the high repro-ducibility of both measurements across the technical replicates suggests that this system could be a potentially useful model for further exploring the relationship between community structure and function.A central question in microbial ecology is to what extent information about the taxonomic composition of microbial assemblages allows prediction of their biogeochemical function (Bier et al., 2015;Graham et al., 2016).Future studies should seek to experimentally test the relative importance of initial population size and the presence or absence of grazers in determining DOM utilization rates.Future work should also consider bacterial community composition dynamics, size selection and DOM structural characteristics across multiple sites and climate settings to confirm the degree to which filtration strategy affects DOM biolability assays across a wider range of catchment settings.

Conclusions
Filtration strategy was shown in this study to affect both microbial community composition and DOM degradation dynamics, but these two responses were disconnected from one another.There are two important conclusions from these results for interpreting aquatic biolability assays.Firstly, our results suggest that care should be taken when comparing results using different filtration strategies, especially for shorter incubations since we have shown that the DOM degradation and microbial community dynamics may not converge until after the commonly used 28-day length for long-term biolability assays, if at all (Figs. 1 and 4).However, in many cases the relevant parameter to be estimated from such longterm incubations is the total biolabile fraction (Guillemette and del Giorgio, 2011), and despite the divergent dynamics in the early part of our incubation it appears that all filtration strategies do eventually converge on a similar value for total DOM biolability.The P7 (0.7 µm filtration with no inoculum) treatment appeared to reach this point at the fastest rate.
Data availability.All sequence data are available on the NCBI Sequence Read Archive under BioProject ID PRJNA507183.All DOC and DOM data are provided in the Excel spreadsheet in the Supplement.
Author contributions.JFD, JTW, RA and HD conceived and designed the study.JFD, JTW and JRvH carried out the experiment and laboratory analyses.JFD and JTW analysed and interpreted the data.JFD and JTW wrote the manuscript.All authors contributed to draft versions of the manuscript prior to submission.
Figure1.DOC concentration dynamics over the course of the incubations for the three filtration treatments.The thicker smoothed lines represent the best-fit GAM as selected using AIC (see Sect. 2.6); thinner lines define the bounds of the 95 % prediction intervals derived from the model fits, with standard error estimates pooled across all observations (Table1).

Figure 2 .
Figure 2. δ 13 C-DOC over the course of the incubation.UF values are the same as P7 for day 0 because the DOC subsamples were treated in the same manner, so a further set of technical replicates was deemed unnecessary (see Sect. 2.3).
Figure  4aand b display the relationships between bacterial community profiles based on treatment and sampling time.There is a clear temporal shift in bacterial community composition, the trajectory of which varies due to filtration treatment.

Figure 3 .
Figure 3. DOM quality (structural proxies) dynamics during the course of the incubations for each filtration treatment, separated into the seven different DOM structural indices used in this study (a-g; see Sect.2.3).Points represent individual replicates; lines connect mean values calculated for each day × treatment combination (Table2).

Figure 4 .
Figure 4. Bacterial dynamics for each of the filter treatments during the incubations.(a, b) NMDS ordinations based on presence-absence (a) or abundance-weighted (b) UniFrac distances; clusters (convex hulls) for each day × treatment combination are plotted; the lines join the centre of each cluster following the sequence of sampling.(c) Relative read abundances of the nine most abundant bacterial (sub-) phyla, based on 16S rRNA gene amplicon sequence data, separated by sampling time and treatment.Each column represents an experimental replicate."Other" contains both unassigned bacterial reads and reads assigned to less abundant taxa.See Supplement for univariate time series plots for the most abundant taxonomic groups.
• C for 30 s; 30 cycles of 98 • C for 30 s, 55 • C for 30 s, 72 • C for 30 s; and a final step of 72 • C for 10 min.After confirming successful amplification, PCR products were purified and normalized using Sequalprep plates (Thermofisher, USA) and subject to a second indexing PCR such that each sample received a unique combination of 6-nucleotide barcoded forward and reverse primers.The reaction mixture was as above, and the PCR programme was an initial step at 95 • C for 30 s; 8 cycles of 95 • C for 30 s, 55 • C for 30 s, 72 • C for 30 s; and a final step of 72 • C for 5 min.PCR products were again purified and normalized with Sequalprep, then pooled, gel purified with QIAquick Gel Extraction Kit (Qiagen, USA), quantified with qPCR and the KAPA Library Quantification Kit, and sequenced on an Illumina MiSeq, with V3 chemistry, 2×300 cycles and a target PhiX concentration of 20 %.

Table 2 .
Analyses of DOM spectral properties, with ANOVA values for each of the DOM spectral indices.Each row represents a different index.Each column indicates the F ratio for the corresponding term in a two-way factorial ANOVA model.Numerator and denominator degrees of freedom are given for each column.Within a row, larger values indicate relatively larger effects.Asterisks indicate significance levels.