Pyrite oxidization accelerates bacterial carbon sequestration in copper mine tailings Type of contribution

Polymetallic mine tailings have great potential as carbon sequestration tools to stabilize atmospheric CO2 10 concentrations. However, previous studies focused on carbonate mineral precipitation, while the role of autotrophs in carbon sequestration by mine tailings has been neglected. In this study, carbon sequestration in two mine tailings treated with FeS2 and 13C-labeled CO2 was analyzed using 13C isotope labeling, pyrosequencing and DNA-based stable isotope probing (SIP) to identify carbon fixers. Mine tailings treated with FeS2 exhibited a higher percentage of 13C atoms (1.76±0.06 in Yangshanchong and 1.36±0.01 in Shuimuchong) than the control groups over a 14-day incubation, 15 as well an increase in the total organic carbon (TOC) content (0.20±0.11 mg/g in Yangshanchong and 0.28±0.14 mg/g in Shuimuchong). These data demonstrated the role of autotrophs in carbon sequestration with pyrite addition. Pyrite treatment led to changes in the composition of bacterial communities, and the genera Sulfobacillus (8.04%) and Novosphingobium (8.60%) were found to be dominant in these communities. In addition, the DNA-SIP results indicated that the cbbL gene copy number was 8.20-16.50 times greater than the cbbM gene copy number in 20 13C-labeled heavy fractions. Furthermore, a Sulfobacillus-like cbbL gene sequence (cbbL-OTU1) accounted for 30.11-34.74% of all cbbL gene sequences in the 13C-labeled heavy fractions of mine tailings treated with FeS2. These findings highlight the importance of the RubisCO form I-encoding gene, cbbL, in bacterial carbon sequestration and demonstrate the ability of chemoautotrophs to sequester carbon during sulfide mineral oxidation in mine tailings. This study is the first to investigate carbon sequestration by autotrophic groups in mine tailings through the use of isotope 25 tracers and DNA-SIP.

Compared with soil ecosystems, polymetallic mine tailings exhibit specific features, including a lack of organic Published by Copernicus Publications on behalf of the European Geosciences Union.574 Y. Li et al.: Pyrite oxidization accelerates bacterial carbon sequestration matter, nutrients, and nutrient-holding capacity (Lottermoser, 2010;Young et al., 2015); these characteristics restrict plant growth, and it is generally difficult to restore plant productivity in mining wastelands (Li et al., 2017;Hu et al., 2018).As the limited amount of organic matter in mine tailings also inhibits the activities of heterotrophic bacteria, the microbes in these environments are dominated by lithotrophs (Li et al., 2015), and these autotrophic bacteria may accordingly play a role in organic carbon sequestration in mine tailings that cannot be ignored.In addition, polymetallic mine tailings have considerable potential to stabilize levels of atmospheric CO 2 (Harrison et al., 2013) through the carbonation of noncarbonate minerals, including dissolution of silicates, hydroxides, and oxides and precipitation of carbonate minerals (McCutcheon et al., 2014(McCutcheon et al., , 2016;;Meyer et al., 2014).However, previous studies have mainly focused on carbonate mineral precipitation, whereas the role of autotrophic bacteria in carbon sequestration by mine tailings has been overlooked.
Polymetallic mine tailings contain sulfide minerals (e.g., pyrite), and oxidation of these sulfide minerals leads to a decrease in pH, also known as mine tailing acidification.Previous studies have noted that due to the limited amount of organic matter present, polymetallic mine tailings have lithotroph-dominated microbial compositions (Li et al., 2015).Consequently, acidophilic, chemoautotrophic bacteria, including Acidithiobacillus, Leptospirillum, and Sulfobacillus (Chen et al., 2013;Liu et al., 2014), largely participate in ferrous and sulfur oxidation in mine tailings, and these autotrophic taxa have leading roles in carbon cycling and energy flow during the mine tailing acidification process.Nonetheless, the relationship between the oxidation of sulfide minerals and carbon sequestration by these acidophilic chemoautotrophic bacteria remains unknown.In the present study, we conducted a microcosm experiment using mine tailings collected from two sites to determine the effects of sulfide mineral (pyrite) oxidation on carbon sequestration in mine tailings through pyrite addition.The main carbon fixers were also examined using DNA-based stable isotope probing (DNA SIP) and cbbL and cbbM gene analysis.Our objectives were to investigate whether sulfide mineral oxidation can stimulate carbon sequestration in mine tailings and to identify key carbon sequestration groups in mine tailings during the acidification process.

Sampling of mine tailings
Samples of mine tailings were collected from the Tongling Yangshanchong (30 • 54 N, 117 • 53 E) and Shuimuchong (30 • 55 N, 117 • 50 E) mine tailing ponds of copper mines in Anhui Province, east China.Samples of oxidized mine tailings on the surface (0-20 cm) were collected using a steel corer in October 2015.The mine tailing samples were placed in sterilized plastic bags, transported to the laboratory in an ice cooler, and stored at −20 • C before analysis.The properties of the mine tailings were as follows: -Yangshanchong acidic samples had a pH of 3.21, total nitrogen (TN) of 0.11 g kg −1 , total organic carbon (TOC) of 16 g kg −1 , SO 2− 4 of 13.32 g kg −1 , As T of 63.29 mg kg −1 , Fe T of 133.46 g kg −1 , Cu T of 1.95 g kg −1 , Pb T of 27.58 mg kg −1 , and Zn T of 205.44 mg kg −1 .

DNA SIP microcosms
A total of four treatments were established using microcosms of the two mine tailings.In the FeS 2 treatment, fresh mine tailings (equivalent to 10.0 g d.w.s., dry weight soil) of each sample were mixed with a total of 2 g of sterile pulverized FeS 2 at approximately 60 % maximum water-holding capacity, followed by incubation at 25 • C in the dark for 14 days.The microcosms were incubated with 10 % 13 C-CO 2 or 12 C-CO 2 , and both treatments were constructed in triplicate for DNA SIP analysis.Yangshanchong mine (YM) tailing samples and Shuimuchong mine (SM) tailings cultured with FeS 2 are abbreviated as YM_FeS 2 and SM_FeS 2 ; fresh mine tailings at approximately 60 % maximum water-holding capacity without any additive were used as the control groups and abbreviated as YM_ck and SM_ck.

Chemical property analysis
Carbon isotope composition was analyzed using a Delta V Advantage mass spectrometer (Thermo Fisher Scientific, Inc., USA) coupled with an elemental analyzer (Flash2000; HT Instruments, Inc., USA) in continuous-flow mode.The 13 C atom % was calculated as follows: The TOC content was assessed using an element analyzer (vario MACRO cube, Elementar Inc., Germany).The carbon isotope composition and TOC content were determined after soil acidification pretreatment to remove inorganic carbon, as described previously (Wang et al., 2015).The pH of the mine tailing samples was measured using a pH meter (tailings : water = 1 g : 5 mL) at the end of the microcosm experiment.Fe 2+ and Fe 3+ in the soils were extracted using HCl.Fe 2+ in the extract was measured using a spectrophotometric method after mixing with phenanthroline and trisodium citrate; Fe 3+ in the extract was reduced to Fe 2+ by hydroxylam- monium chloride and measured using the spectrophotometric method (Heron et al., 1994).The total sulfate ion content was determined via ion chromatography after extraction with sodium hydroxide, as described previously (Yin and Catalan, 2003).

DNA extraction and SIP gradient fractionation
Total DNA was extracted from each sample using the FastDNA ® SPIN Kit for Soil (MP Biomedicals, Cleveland, OH, USA) according to the manufacturer's instructions.DNA-based stable isotope probing (DNA SIP) fractionation was performed as previously described (Zheng et al., 2014), and 14 gradient fractions were generated for each sample.
The refractive index of each fractionated DNA was measured using an AR200 digital handheld refractometer (Reichert, Inc., Buffalo, NY, USA).

Pyrosequencing of the 16S rRNA gene
The composition of the bacterial communities in different samples was assessed by 16S rRNA gene pyrosequencing.The 16S rRNA gene from the 13 C-labeled DNA fraction, with CsCl buoyant densities of 1.738 g mL −1 in the heavy fraction of YM_FeS 2 and 1.734 g mL −1 in the heavy frac-tion of SM_FeS 2 , was also amplified for pyrosequencing.
The primer pair 515F-907R was used for amplification of the V4-V5 regions of the 16S rRNA gene.Primers were tagged with unique bar codes for each sample.Each sample was amplified in triplicate, and the products were pooled.Negative controls using sterilized water instead of soil DNA extract were included to check for primer or sample DNA contamination.The qualities and concentrations of the purified bar-coded PCR products were determined using a NanoDrop spectrophotometer.The bacterial community composition of each sample was assessed by Illumina MiSeq sequencing of the 16S rRNA gene using MiSeq Reagent Kit v3.
Read merging and quality filtering of the raw sequences were performed using QIIME software with the UP-ARSE pipeline.The "identify_chimeric_seqs.py" command was used to identify chimeric sequences according to the UCHIME algorithm, and chimeric sequences were removed with the "filter_fasta.py"command.Operational taxonomic units (OTUs) were clustered with 97 % similarity, and OTU picking and taxonomy assignments were performed with the "pick_de_novo_otus.py"command for subsequent analysis.OTUs containing less than 10 reads in the 13 C-labeled DNA fractions were removed.The raw amplicon sequence data for the 16S rRNA gene have been deposited in the GenBank sequence read archive under accession number SRP155504.

Clone library construction of the cbbL and cbbM genes
Clone libraries of the cbbL and cbbM genes were also constructed using 13 C-labeled DNA fractions with CsCl buoyant densities of 1.738 g mL −1 in the YM_FeS 2 heavy fraction and 1.734 g mL −1 in the SM_FeS 2 heavy fraction.Primer pairs K2f-V2r and cbbMF-cbbMR were used to amplify the cbbL and cbbM genes, respectively.Triplicate amplicons were pooled, ligated to the pGEM-T vector (Promega, Fitchburg, WI, USA), and transformed into competent DH5.α cells.Sanger sequencing of randomly selected positive clones reveals 188 cbbL gene sequences and 183 cbbM gene sequences.OTU clustering with 97 % similarity was performed using mothur.Representative OTU sequences of the cbbL and cbbM genes obtained from clone library sequencing have been deposited in GenBank under accession numbers MH699091 to MH699105.

Data analysis
Bray-Curtis distance matrices for the overall bacterial community composition among the samples were calculated in R v.3.3.2 using the "vegdist" function of the vegan package and visualized by nonmetric multidimensional scaling (NMDS) in Origin 8.A heat map of dominant genera with relative abundances above 0.02 % was applied for plotting in the R environment using the pheatmap package.Translated cbbL and cbbM sequences from the heavy fractions were used to construct a phylogenetic tree with the neighborjoining method using the MEGA package, version 7.0.

Pyrite oxidation and carbon sequestration
No significant changes in chemical properties, pH values (3.25 ± 0.09 in YM_ck and 2.98 ± 0.04 in SM_ck), sulfate (SO 2− 4 ) contents (13.15 ± 2.58 mg g −1 in YM_ck and 8.95 ± 2.19 mg g −1 in SM_ck), and TOC contents (16.75 ± 0.09 mg g −1 in YM_ck and 18.55 ± 0.12 mg g −1 in SM_ck) were found for the control groups compared to the original Yangshanchong and Shuimuchong acidic samples after 14 days of incubation (Fig. 1).However, the addition of pyrite decreased pH values in the YM and SM samples by 0.48 ± 0.16 and 0.41 ± 0.07, respectively.Pyrite addition also increased the SO 2− 4 content by 252.96 % and 262.35 %, Fe 2+ content by 329.47 % and 240.38 %, and Fe 3+ content by 137.47 % and 140.37 % in the YM and SM samples, respectively.Together, these data indicate the occurrence of pyrite oxidization and acidification in mine tailings after pyrite addition.Additionally, the TOC content increased by 0.20 ± 0.11 mg g −1 in YM_FeS 2 and 0.28 ± 0.14 mg g −1 in SM_FeS 2 , and the 13 C atom % values in YM_FeS 2 (1.76 ± 0.06 13 C atom %) and SM_FeS 2 (1.76 ± 0.06 13 C atom %) were higher than those in the controls YM_ck (1.12 ± 0.01 13 C atom %) and SM_ck (1.11±0.01 13 C atom %).This result shows that fixation of 13 C-CO 2 occurred in these mine tailings with the addition of pyrite; the CO 2 -fixing capacities of autotrophs under FeS 2 addition were 9.50 ± 0.91 mg kg −1 d −1 in YM and 3.69 ± 0.11 mg kg −1 d −1 in SM.For each tailing sample, the relative abundances of the sequences assigned to a given taxonomic unit were calculated for each of three subsamples, and the average value was then used to represent the relative abundance of each tailing sample.

DNA SIP identification of autotrophs in mine tailings
For quantitative analysis of cbbL and cbbM gene abundances, the buoyant densities of the DNA in isopycnic centrifugation gradients were employed to assess the labeling efficiencies of cbbL or cbbM gene-carrying carbon fixers in the DNA SIP microcosms (Fig. 5).cbbL and cbbM gene levels under 13 C-CO 2 treatment peaked at a density of 1.72 g mL −1 in both the 12 C-CO 2 and 13 C-CO 2 treatments.In addition, a shift toward heavy fractions was observed for cbbL and cbbM gene abundances in the 13 C-CO 2 treatment (Fig. 5), with buoyant densities of 1.738 g mL −1 in YM_FeS 2 and 1.734 g mL −1 in SM_FeS 2 .In contrast, the highest copy numbers of cbbL and cbbM genes under the 12 C-CO 2 treatment appeared in the light fraction, with a buoyant density of 1.722-1.723g mL −1 .cbbL and cbbM sequences, encoding the large subunit of RuBisCO, from the clone libraries in the 13 C-DNA heavy fraction treated with 13 C-CO 2 were used for phylogenetic analysis (Fig. 6).The gene copy numbers of cbbL were 16.50-fold and 8.20-fold greater than those of cbbM in the heavy fractions of YM_FeS 2 and SM_FeS 2 , respectively.In addition, a vast majority of cbbL and cbbM gene sequences appear to be associated with unknown groups, with the exception of cbbL-OTU1, cbbL-OTU6, cbbL-OTU9, cbbM-OTU5, and cbbM-OTU6.According to phylogenetic analysis based on amino acid sequences, the cbbL gene OTUs are related to Sulfobacillus, Acidithiobacillus, and Azospirillum and the cbbM gene OTUs are related to Acidithiobacillus and Thiobacillus.The Sulfobacillus-like cbbL gene sequence cbbL-OTU1 accounted for 30.11 % and 34.74 % of the total cbbL gene sequences in the heavy fractions of YM_FeS 2 and SM_ FeS 2 , respectively.Conversely, Acidithiobacilluslike cbbL and cbbM genes accounted for only 2.15 % and 4.21 % of the total cbbL gene sequences and 3.30 % and 4.35 % of the total cbbM gene sequences in the heavy fractions of YM_ FeS 2 and SM_FeS 2 , respectively.In addition, the genus Sulfobacillus exhibited the highest relative abundance based on 16S rRNA analysis (Table S2), accounting for 17.18 % and 18.24 % of the heavy fractions of YM_FeS 2 and SM_FeS 2 , respectively.The Leptospirillum genus accounted for 1.32 % and 1.58 % of the heavy fractions of YM_FeS 2 and SM_FeS 2 , respectively, and Acidithiobacillus accounted for 0.11 % and 0.06 % in the heavy fractions of YM_FeS 2 and SM_FeS 2 , respectively.

The effect of FeS 2 on the entire bacterial community in mine tailings
Acidic polymetallic mine tailings have strong potential for pyrite oxidation.In this study, a large amount of sulfuric acid was generated (increases of approximately 19.95 and 14.64 mg g −1 in YM_FeS 2 and SM_FeS 2 , respectively), with a persistent decline in pH (decreases by 0.44 and 0.35 in YM_FeS 2 and SM_FeS 2 , respectively), in only 14 days.These changes clearly indicate the oxidization of pyrite (i.e., acidification) in these mine tailings.Previous studies have found significant increases in certain bacterial phyla, such as Firmicutes and Nitrospirae (Chen et al., 2013;Liu et al., 2014), with the acidification process of mine tailings.In the present study, the bacterial composition in the different mine tailings varied greatly, with only Firmicutes increasing in both tested mine tailings under pyrite addition.This group might participate in the oxidization of sulfide minerals (Chen et al., 2013), and Sulfobacillus accounted for the majority of Firmicutes.However, many other microorganisms might be inhibited under pyrite addition.Korehi et al. (2014) and Liu et al. (2014) also indicated that the ongoing oxidation  S1.
process in mine tailings was accompanied by an increase in Firmicutes and a decrease in Actinobacteria as well as all classes of Proteobacteria except Gammaproteobacteria.In addition, Chen et al. (2013) and Liu et al. (2014) reported relative abundances of Euryarchaeota belonging to archaea significantly increased with decreasing pH, which indicates that this taxon is an indicator of metal contamination (Hur et al., 2011).Euryarchaeota compete with Betaproteobacteria for ecological niches under such acidic conditions (Liu et al., 2014).However, in this study archaea have been ignored, which will require further study.
The growth of microorganisms in bare mine tailings is usually limited by the availability of organic carbon (Schimel and Weintraub, 2003).In our study, pyrite oxidization in mine tailings further increased the acidity of the mine tailings (pH decreased to 2.77 and 2.57 in YM_FeS 2 and SM_FeS 2 , respectively).As a result, only microorganisms that were able to overcome resistance to infertility and/or acidophilic conditions maintained high activities.The level of some specific taxa, including the autotrophic genera Acidithiobacillus and Sulfobacillus, increased in both of the tested mine tailings under pyrite addition (Fig. 4), indicating high consistency of dominant autotrophic bacterial genera in different mine tailings.It is possible that acidophilic and/or autotrophic bacteria might be stimulated under conditions of pyrite oxidization and the availability of organic carbon (Deng et al., 2016;Antonelli et al., 2018); moreover, the main carbon fixers in these two mine tailings may be derived from the same groups.In addition, levels of major ferrous and sulfur-oxidizing genera, autotrophic Leptospirillum and Acidiphilium in YM_ FeS 2 and autotrophic Thiobacillus in SM_FeS 2 , were enhanced, suggesting the synchronization of pyrite oxidization and carbon sequestration in mine tailings.

The effect of FeS 2 on bacterial carbon sequestration in mine tailings
Previous studies have shown that mine tailings provide an excellent substrate for carbon sequestration through the formation of carbonate due to the large surface area of the material grains (McCutcheon et al., 2016).Compared to soils and natural bedrock, mine tailings may exhibit higher carbonate precipitation rates (Wilson et al., 2009).Although the results showed that TOC content in mine tailings increased slightly under FeS 2 addition, the soil acidification pretreatment and the addition of 20 % FeS 2 in samples could increase the error of TOC analysis and calculation.And a longterm field test should be used to calculate the TOC increment in the acidification process of mine tailings in the future.Even so, in this study, the 13 C content in mine tailings increased significantly (Fig. 1).DNA SIP analysis demonstrated assimilation of a considerable amount of 13 C-CO 2 by carbon fixers in the 13 C-CO 2 -labeled mine tailing samples, leading to a significant shift in cbbL or cbbM gene-carrying genomic DNA into the heavy fraction.In addition, a peak at a buoyant density of 1.72 g mL −1 in 13 C-CO 2 -labeled mine tailing samples was observed, with a density lower than the peak in the 12 C-CO 2 -labeled control experiment (see Supplement); the intensity of the 13 C peak at a higher density of 1.738 g mL −1 was also much lower than the 12 C peak at a higher density of 1.72 g mL −1 .This suggests that a large proportion of the autotrophic microorganisms detected in the mine tailing samples did not fix atmospheric carbon.All of these results indicate the contribution of special autotrophic bacteria to carbon sequestration in mine tailings.To the best of our present knowledge, this report is the first to investigate carbon sequestration by autotrophic groups in mine tailings based on isotope tracers and DNA SIP.Previous studies have found that microbial photosynthesis accelerates car-bonate mineral precipitation and further induces mineralization (McCutcheon et al., 2014(McCutcheon et al., , 2016)).In the present study, however, the microcosms were not cultured in the presence of illumination, and as a result, chemoautotrophic microorganisms, particularly iron and/or sulfide oxidizers (such as Sulfobacillus), were the dominant carbon fixers.These results indicate that bacterial carbon sequestration is mainly attributable to chemoautotrophic bacteria in mine tailings during pyrite oxidization.Nonetheless, archaea may have higher activities in RuBisCO-mediated carbon metabolic pathways (Kono et al., 2017), which will require further study.
During pyrite oxidization in mine tailings, some acidophilic autotrophic microorganisms showed very high activity levels; for example, levels of Sulfobacillus and Leptospirillum, both of which are vital ferrous and sulfur oxidizers, increased significantly.Zhang et al. (2016) identified genes for the CBB pathway and rTCA, but no other CO 2 fixation pathways, in a copper bioleaching microbial community.Regarding the present study, the Sulfobacilluslike cbbL gene was the major carbon-fixing-associated gene found.Ňancucheo and Johnson (2012) reported that among acidophilic prokaryotes isolated from mine-impacted environments, the ability to metabolize glycerate-3-phosphate appeared to be restricted to Firmicutes (e.g., Sulfobacillus) and that the glycerate-3-phosphate present in all of these acidophiles might be due to the activity of RuBisCO ( Ňancucheo and Johnson, 2012).Previous studies have confirmed the existence of Sulfobacillus in mine tailings (Coral et al., 2018;Yu et al., 2018), and members of this genus have the ability to oxidize or reduce Fe(III) and oxidize sulfur (Dold et al., 2005).This ability is important because by adhering to mineral surfaces and further enhancing sulfide mineral oxidation, Sulfobacillus likely leads to a high mineral dissolution rate (Becker et al., 2011;Li et al., 2016).Although none of the cbbL or cbbM genes identified in our study were highly homologous to genes in Leptospirillum, Marín et al. (2017) reported that rTCA carbon fixation pathway genes were mainly found in Leptospirillum spp.RuBisCO is the most prominent enzyme, and the genes encoding the large subunit of RuBisCO serve as a marker for the analysis of autotrophic organisms, including bacteria, using the CBB cycle (Berg, 2011).The Sulfobacillus-like cbbL gene dominated the 13 C-labeled DNA among carbon-fixing taxa, and the higher relative abundance of Sulfobacillus than Leptospirillum, according to 16S rRNA analysis, demonstrates the contribution of the Sulfobacillus-like cbbL gene to carbon sequestration.Regardless, although the number (or relative abundance) of autotrophs demonstrated their ability to sequester carbon, it did not reflect their ability to perform or their importance to ferrous and sulfur oxidation.For example, the limited percentage of Acidithiobacillus in the two mine tailings did not reflect the contribution of this genus to the oxidation of iron and sulfur.Falagán et al. (2017) highlighted the importance of thermotolerant acidophiles, such as Acidithiobacillus and Sulfobacillus, in extracting and re-covering metals from mine tailings.Furthermore, it has been known for many years that Acidithiobacillus can obtain energy by catalyzing oxidation of Fe 2+ to Fe 3+ from sulfites (Dold, 2014), which may significantly accelerate the rate of ferrous oxidization.The decreased level of total carbon, including low-molecular-weight carboxylic acids, may also limit the activity of this taxon (Dold et al., 2005).
In conclusion, this study is the first to elucidate carbon sequestration by autotrophic groups in the mine tailing acidification process based on isotope tracers and DNA SIP.Our results reveal higher 13 C atom % values with the addition of pyrite than in controls after a 14-day incubation.The Sulfobacillus genus was dominant in the pyrite-treated bacterial communities and was also the primary carbon fixer carrying the cbbL gene.Overall, the cbbL gene may play a vital role in carbon sequestration in the sulfide mineral oxidation of mine tailings.

Figure 2 .
Figure 2. Nonmetric multidimensional scaling (NMDS) of the overall bacterial community composition according to Bray-Curtis distance matrices in mine tailings.

Figure 3 .
Figure3.Relative abundances (percentages) of the main bacterial taxonomic groups identified, i.e., Proteobacteria, Firmicutes, Acidobacteria, Actinobacteria, candidate division WPS-2, Planctomycetes, candidate division AD3, and Nitrospirae (a), and classes Alphaproteobacteria, Betaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria (within the phylum Proteobacteria) (b).For each tailing sample, the relative abundances of the sequences assigned to a given taxonomic unit were calculated for each of three subsamples, and the average value was then used to represent the relative abundance of each tailing sample.

Figure 4 .
Figure 4. Heat map of the top genera with relative abundances above 0.02 % in mine tailings.Autotrophic bacteria were marked with underlining.

Figure 5 .
Figure 5. Quantitative distribution of cbbL and cbbM gene fragments across the entire buoyant density gradients of the DNA fractions from microcosms treated with FeS 2 and incubated with 12 C-CO 2 or 13 C-CO 2 .The normalized data consist of the ratio of the gene copy number for each DNA gradient to the maximum quantity for each treatment.The error bars represent the standard errors of triplicate microcosms, and each consisted of three technical replicates.cbbL and cbbM gene abundance qPCR data are presented in the Supplement.

Figure 6 .
Figure 6.Phylogenetic tree of translated cbbL and cbbM sequences in the heavy fractions from YM and SM treated with FeS 2 .Relative frequencies (%) are marked in the bar graph.Bootstrap values of > 50 % are indicated at branch points.cbbL and cbbM gene copy numbers in the heavy fractions from FeS 2 -treated YM and SM are shown in the middle of the figure.The cultured genera most related to OTUs from cbbL and cbbM clone libraries are shown in TableS1.