References

BGD

Biogeosciences Discussions

BGD

Biogeosciences Discuss.

1810-6285

Göttingen, Germany

10.5194/bgd-9-5929-2012

Primary Productivity and heterotrophic activity in an enclosed marine area of central Patagonia (Puyuhuapi channel; 44° S, 73° W)

Daneri

¹ ³ ⁶ Montero

¹ ³ ⁶ Lizárraga

² ³ Torres

¹ Iriarte

J. L.

¹ ³ ⁵ ⁶ Jacob

² ⁶ ⁷ González

H. E.

¹ ³ ⁴ ⁶ Tapia

F. J.

³ ⁶

Centro de Investigación en Ecosistemas de la Patagonia (CIEP), Bilbao 449, Coyhaique, Chile

Centro de Ciencias y Ecología Aplicada (CEA), Universidad del Mar, Carmen 446, Cerro Placeres, Valparaíso, Chile

Centro de Investigaciones Oceanográficas del Pacífico Sudoriental (COPAS), Universidad de Concepción, Casilla 160-C, Concepción, Chile

Instituto de Biología Marina, Universidad Austral de Chile, Casilla 567, Valdivia, Chile

Instituto de Acuicultura, Universidad Austral de Chile, Puerto Montt, Chile

Centro BASAL COPAS Sur-Austral, Universidad de Concepción, Centro de Investigación en Ecosistemas de la Patagonia, Chile

Programa de Postgrado en Oceanografía, Departamento de Oceanografía, Universidad de Concepción, Casilla 160-C, Concepción, Chile

25 05 2012

9 5 5929 5968

2012

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This article is available from https://bg.copernicus.org/preprints/9/5929/2012/bgd-9-5929-2012.html

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We assessed temporal variability in phytoplankton biomass, Chlorophyll a, nutrient availability, Gross Primary Production (GPP), community respiration (CR), and bacterial secondary production (BSP) over a year of monthly observations (October 2007 to October 2008) at a fixed station in the Puyuhuapi fjord, Chilean Patagonia (44° S, 73° W). A set of in situ observations gathered over two consecutive spring-summer seasons, and one autumn-winter season in the middle, has made it possible to connect the two-phase (i.e. productive season/non-productive season) pattern of Chlorophyll a (Chl a) variability shown by satellite data with a two-phase cycle in GPP, CR, and the composition of phytoplankton assemblages. Estimates of annual GPP and CR, integrated over the top 20 meters of the water column, were 533 and 537 g C m−2 yr−1, respectively. Low values of pCO2 were measured in mixed layer autotrophic waters (GPP/CR > 1) while high pCO2 levels were measured in mixed layer heterotrophic waters (GPP/CR < 1). Bacterial Secondary Production (BSP) was significantly and positively correlated with GPP (r = 0.6, p < 0.05, n = 24) and Chl a (r = 0.4, p < 0.05, n = 24) on an annual cycle basis. The winter drop in bacterioplankton (both bacteria and archea) activity (from 0.9 ± 0.6 g C m−2 d−1 to 0.6 ± 0.3 g C m−2 d−1) was not as pronounced as the winter drop in phytoplankton activity (from 1.1 ± 1.12 g C m−2 d−1 to 0.1 ± 0.09 g C m−2 d−1). It is hypothesized that dissolved organic matter (DOM) of terrestrial origin plays an important role (especially in winter) supporting bacterial activity in the Puyuhuapi fjord.

References 1

Acha, E. M., Mianzan, H. W., Guerrero, R. A., Favero, M., and Bava, J.: Marine fronts at the continental shelves of austral South America: Physical and ecological processes, J. Mar. Syst., 44, 83–105, 2004.

Albright, L. J. and McCrae, S. K.: Annual Bacterioplankton biomasses and productivities in a temperate west coast Canadian Fjord, Appl. Environ. Microb., 53, 1277–1285, 1987.

Alves-de-Souza, C., González, M. T., and Iriarte, J. L.: Functional groups in marine phytoplankton assemblages dominated by diatoms in fjords of southern Chile, J. Plankton Res., 30, 1233–1243, 2008.

Aristegui, J. and Harrison, W. G.: Decoupling of primary production and community respiration in the ocean: implications for regional carbon studies, Mar. Ecol.-Prog. Ser., 29, 199–209, 2002.

Blight, S. P., Bentley, T. L., Lefevre, D., Robinson, C., Rodriguez, R., Rowlands, J., and Williams, P. J. Leb.: Phasing of autotrophic and heterotrophic plankton metabolism in a temperate coastal ecosystem, Mar. Ecol.-Prog. Ser., 128, 61–75, 1995.

Bukaveckas, P. A., Williams, J. J., and Hendricks, S. P.: Factors regulating autotrophy and heterotrophy in the main channel and an embayment of a large river impoundment, Aquat. Ecol., 36, 355–369, 2002.

Button, D. K.: Evidence for a Terpene-based food chain in the Gulf of Alaska, Appl. Environ. Microb., 48, 1004–1011, 1984.

Carlson, C. A., del Giorgio, P. A., and Herndl, G. J.: Microbes and the dissipation of energy and respiration: from cell to ecosystems, Oceanography, 20, 89–100, 2007.

Chaigneau, A. and Pizarro, O.: Mean surface circulation and mesoscale turbulent flow characteristics in the eastern South Pacific from satellite tracked drifters, J. Geophys. Res., 110, C05014, <a href="http://dx.doi.org/10.1029/2004JC002628">https://doi.org/10.1029/2004JC002628</a>, 2005.

Cole, J. J., Findlay, S., and Pace, M. L.: Bacterial production in fresh and saltwater ecosystems: a cross-system overview, Mar. Ecol. Prog. Ser., 43, 1–10, 1988.

Cloern, J. E.: Does the benthos control phytoplankton biomass in South San Francisco Bay?, Mar. Ecol. Progr. Ser., 9, 191–202, 1982.

Cuevas, L. A., Daneri, G., Jacob, B., and Montero, P.: Microbial abundance and activity in the seasonal upwelling area off Concepción ($\sim $36°S), central Chile: a comparison of upwelling and non-upwelling conditions, Deep-Sea Res. II., 51, 2427–2440, 2004.

Daneri, G., Dellarossa, V., Quiñones, R., Jacob, B., Montero, P., and Ulloa, O.: Primary production and community respiration in the Humboldt Current System off Chile and associated oceanic areas, Mar. Ecol. Prog. Ser., 197, 41–49, 2000.

del Giorgio, P. A. and Cole, J.: Bacterial growth efficiency in natural aquatic systems, Annu. Rev. Ecol. Syst., 29, 503–541, 1998.

del Giorgio, P. A. and Duarte, C. M.: Respiration in the open ocean, Nature, 420, 379–384, 2002.

del Giorgio, P. A. and Peters, R.: Balance between phytoplankton production and plankton respiration in lakes, Can. J. Fish. Acquat. Sci., 50, 282–289, 1993.

Dickson, A. G. and Millero, F. J.: A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media, Deep-Sea Res., 34, 1733–1743, 1987.

DOE: Handbook of Methods for Analysis of the Various Parameters of the Carbon Dioxide System in Sea Water, Version 2, edited by: Dickson, A. G. and Goyet, C., unpublished manuscript, 1994.

Eppley, R. W., Rogers, J. N., and McCarthy, J. J.: Half-saturation constants for uptake of nitrate and ammonium by marine phytoplankton, Limnol. Oceanogr., 14, 912–920, 1969

Farmer, D. M. and Freeland, H. J.: The physical oceanography of fjords, Progr. Oceanogr., 12, 147–220, 1983.

Fehling, J., Davidson, K., and Stephensen, B.: Growth dynamics of non toxics Pseudo-nitzschia delicatissima and toxic P. seriata (Bacillariophyceae) under simulated spring and summer photoperiods, Harmful Algae, 4, 763–769, 2005.

Findlay, S., Pace, M. L., Lints, D., Cole, J. J., Caraco, N. F., and Peierls, B.: Weak coupling of bacteria and algal production in a heterotrophic ecosystem: the Hudson River estuary, Limnol. Oceanogr., 36, 268–278, 1991.

Gibbs, M. T.: Aspects of the structure and variability of the low-salinity-layer in Doubtful sound, a new Zealand fjord, New Zeal. J. Mar. Fresh., 35, 59–72, 2001.

Goebel, N. L., Wing, S. R., and Boyd, P. W.: A mechanism for onset of diatom blooms in a fjord with persistent salinity stratification, Est. Coast. Shelf Sci., 64, 546–560, 2005.

González, H. E., Calderon, M. J., Castro, L., Clement, A., Cuevas, L. A., Daneri, G., Iriarte, J. L., Lizárraga, L., Matínez, R., Menschel, E., Silva, N., Carrasco, C., Valenzuela, C., Vargas, C. A., and Molinet, C.: Primary Production and plankton dynamics in the Reloncavi Fjord and the Interior Sea of Chiloe, Northern Patagonia, Chile, Mar. Ecol. Prog. Ser., 402, 13–30, 2010.

Haraldsson, C., Anderson, L. G., Hassellöv, M., Hulth, S., and Olsson, K.: Rapid, high precision potentiometric titration of alkalinity in ocean and sediment pore waters, Deep-Sea Res. I, 44, 2031–2044, 1997.

Hoch, M. P. and Kirchman, D. L.: Seasonal and inter-annual variability in bacterial production and biomass in a temperate estuary, Mar. Ecol. Prog. Ser., 98, 283–295, 1993.

Iriarte, J. L. and Gonzalez, H. E.: Phytoplankton bloom ecology of the inner Sea of Chiloé, Southern Chile, Nova Hedwigia, Beiheft, 133, 67–79, 2008.

Iriarte, J. L., Kush, A., and Ruiz, M.: Phytoplankton biomass in the sub-Antarctic area of the Straits of Magellan (53°S); Chile during spring–summer 1997–1998, Polar Biol., 24, 154–162, 2001.

Iriarte, J. L., González, H. E., Liu, K. K., Rivas, C., and Valenzuela, C.: Spatial and temporal variability of Chlorophyll and primary productivity in surface waters of southern Chile (41.5-43°S), Estuar. Coast. Shelf S., 74, 471–480, 2007.

Kritzberg, E. S., Cole, J. J., Pace, M. M., and Granéli, W.: Does autochthonous primary production drive variability in bacterial metabolism and growth efficiency in lakes dominated by terrestrial C inputs?, Aquat. Microb. Ecol., 38, 103–111, 2005.

Legendre, L. and Rivkin, R. B.: Fluxes of carbon in the upper ocean: regulations by food-webs control nodes, Mar. Ecol. Prog. Ser., 242, 95–109, 2002.

Legendre, L. and Rivkin, R. B.: Planktonic food webs: microbial hub approach, Mar. Ecol. Prog. Ser., 365, 289–309, 2008.

Lewis, E. and Wallace, D. W. R.: Program Developed for CO2 System Calculations. ORNL/CDIAC-105, Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, US Department of Energy, Oak Ridge, Tennessee, 1998.

McManus, G. and Peterson, W. T.: Bacterioplankton production in the nearshore zone during upwelling off central Chile, Mar. Ecol. Prog. Ser., 43, 11–17, 1988.

Mehrbach, C., Culberson, C. H., Hawley, J. E., and Pytkowicz, R. M.: Measurement of the apparent dissociation constants of carbonic acid in seawater at atmospheric pressure, Limnol. Oceanogr., 18, 897–907, 1973.

Montero, P., Daneri, G., Cuevas, L. A., González, H. E., Jacob, B., Lizárraga, L., and Menschel, E.: Productivity cycles in the coastal upwelling area of Concepción: The importance of diatoms and bacteria in the flux of organic carbon, Prog. Oceanogr., 75, 518–530, 2007.

Montero, P., Daneri, G., González, H. E., Iriarte, J. L., Tapia, F. J., Lizárraga, L., Sanchez, N., and Pizarro, O.: Seasonal variability of primary production in a fjord ecosystem of the Chilean Patagonia: implications for the transfer of carbon within pelagic food webs, Cont. Shelf Res., 31, 202–215, 2011.

Newell, S. Y. and Christian, R. R.: Frequency of dividing cells as an estimator of bacterial productivity, Appl. Environ. Microb., 42, 23–31, 1981.

Parsons, T. R., Maita, R., and Lalli, C. M.: Counting, media and preservatives. A manual of chemical and biological methods for seawater analysis (1–163), Pergamon Press, Toronto, 1984.

Pickard, G. L.: Some physical oceanographic features of inlets of Chile, J. Fish. Bd. Canada, 28, 1077–1106, 1971.

Pizarro, G., Iriarte, J. L., Montecino, V., Blanco, J. L., and Guzman, L.: Distribución de la biomasa fitoplanctónica y productividad primaria máxima de fiordos y canales australes (47°–50° S) en octubre 1996, Ciencia y tecnología del 23 Marzo, 25–48, 2000.

Pizarro, G., Astoreca, R., Montecino, Paredes, M. A., Alarcon, G., Uribe, P., and Guzman, L.: Patrones espaciales de la abundancia de la clorofila, su relacion con la productividad primaria y la estructura de tamaños del fitoplancton en Julio y Noviembre de 2001 en la region de Aysén (43°–56°S), Rev. Cien. Tecn. Mar., 28, 27–42, 2005.

Poole, H. H. and Atkins, W. R. G.: Photo-electric measurements of submarine illumination throughout the year, J. Mar. Biol. Ass., UK, 16, 297–324, 1929.

Riemann, B. and Bell, R. T.: Advances in estimating bacterial biomass and growth in aquatic systems, Arch. Hydrobiol., 25, 385–402, 1990.

Rivkin, R. B. and Legendre, L.: Biogenic carbon cycling in the upper ocean: effects of microbial respiration, Science, 291, 2398–2400, 2001.

Robinson, C. and Williams, P. J. LeB.: Respiration and its measurement in surface marine waters, in: Respiration in Aquatic Ecosystems, edited by: del Giorgio, P. A. and Williams, P. J. LleB., Oxford University Press, 147–180, 2005.

Robinson, C.: Heterotrophic bacterial respiration, in: Microbial Ecology of the Ocean, edited by: Kirchman, D. L., 2 Edn., 299–334, 2008.

Sciremammano, F.: A suggestion for the presentation of correlations and their significance levels, J. Phys. Oceanogr., 9, 1273–1276, 1979.

Serret, P., Robinson, C., Fernández, E., Teira, E., Tilstone, G., and Pérez, V.: Predicting plankton net community production in the Atlantic Ocean, Deep-Sea Res. II, 56, 941–953, 2009.

Silva, N.: Dissolved oxygen, pH, and nutrients in the austral Chilean channels and fjords. Progress in the oceanographic Knowledge of Chilean interior waters, from Puerto Montt to Cape Horn, edited by: Silva, N. and Palma, S., Comité Oceanográfico Nacional – Pontificia Universidad Católica de Valparaíso, Valparaíso, 37–43, 2008.

Silva, N., Calvete, M., and Sievers, H.: Características oceanográficas físicas y químicas de canales australes chilenos entre Puerto Montt y Laguna san Rafael (Crucero CIMAR-Fiordo 1), Cienc. Tecnol. Mar., 20, 23–106, 1997.

Simon, M. and Azam, F.: Protein content and protein synthesis rates of planktonic marine bacteria, Mar. Ecol. Prog. Ser., 51, 201–213, 1989.

Strickland, J. D. H.: Measuring the production of marine phytoplankton, Bull. Fish. Res. Board Can., 122, 1–72, 1960.

Strickland, J. D. H. and Parsons, T. R.: Determination of reactive nitrite, in: A Practical Handbook of Seawater Analysis, Bull. Fish. Res. Board Can., 167, 71–75, 1968.

Torres, R., Pantoja, S., Harada, N., González, H., Daneri, G., and Frangopulos, M.: Air-sea CO2 fluxes along the coast of Chile: from CO2 outgassing in central-northern upwelling waters to CO2 sequestering in southern Patagonian fjords, J. Geophys. Res., 116, C09006, <a href="http://dx.doi.org/10.1029/2010JC006344">https://doi.org/10.1029/2010JC006344</a>,

Troncoso, V. A., Daneri, G., Cuevas, L. A., Jacob, B., and Montero, P.: Bacterial carbon flow in the Humboldt Current System off Chile, Mar. Ecol. Prog. Ser., 250, 1–12, 2003.

Utermöhl, H.: Zur Vervollkommnung der quantitativen Phytoplankton. Methodik, Mitt. Int. Ver. Theor. Angew. Limnol., 9, 1–39, 1958.

Williams, P. J. LeB.: The balance of plankton respiration and the photosynthesis in the open ocean, Nature, 394, 55–57, 1998.

Williams, P. J. LeB. and Robertson, J. E.: Overall planktonic oxygen and carbon dioxide metabolisms: the problem of reconciling observations and calculations of photosynthetic quotients, J. Plankton Res., 13, 153–169, 1991.