08 Jul 2020

08 Jul 2020

Review status: a revised version of this preprint is currently under review for the journal BG.

Differential analysis of prokaryotic communities from pristine mangrove tidal zone sediments reveal distinct structures and functional profiles

Carolina Oliveira de Santana1, Pieter Spealman2, Vânia Maria Maciel Melo3, David Gresham2, Taíse Bomfim de Jesus4, and Fabio Alexandre Chinalia1 Carolina Oliveira de Santana et al.
  • 1Programa de Pós-Graduação em Geoquímica: Petróleo e Meio Ambiente, Instituto de Geociências (IGEO), Universidade Federal da Bahia (UFBA), R. Barão de Jeremoabo, s/n - Ondina, Salvador, BA 40170-290, Brazil
  • 2Center for Genomics and Systems Biology, Department of Biology, New York University, New York, New York, United States of America
  • 3Laboratório de Ecologia Microbiana e Biotecnologia (LEMBiotech), Departamento de Biologia, Centro de Ciências, Universidade Federal do Ceará, Campus do Pici, Bloco 909, Avenida Mister Hull s/n, 60.455-970 Fortaleza, CE, Brazil
  • 4Programa de Pós-Graduação em Modelagem em Ciências da Terra e do Ambiente (PPGM), Universidade Estadual de Feira de Santana (UEFS), Avenida Transnordestina, s/n - Novo Horizonte, Feira de Santana, BA 44036-900, Brazil

Abstract. Mangrove forests are intertidal ecosystems that constitute a large portion of the world's coastline, as such, they are composed of, and reliant upon, microhabitats defined by the tides. However, we are only beginning to understand tidal microhabitat biodiversity and their role in nutrient cycling. The majority of metagenomic studies have so far been conducted on anthropogenically impacted areas. As even mild disruption can severely alter ecosystems and lead to decreased biodiversity and local extinctions, this is a critical issue. Here, we characterize prokaryotic populations and their involvement in nutrient cycling across the tidal zones of a pristine mangrove forest within a Brazilian Environmental Protection Area of the Atlantic Forest. We hypothesize that tidal zones in pristine mangroves constitute distinct microhabitats, are composed of different prokaryotic communities and, consequently, distinct functional profiles. Samples were collected in triplicate from zones below, between, and above the tidal waterline. Using 16S amplicon sequencing, we find significantly different prokaryotic communities with diverse nutrient cycling related functions, as well specific taxa with varying contribution to functional abundances between zones. Our findings contrast those observed in anthropogenically impacted mangroves and suggest that some aspects of mangrove zonation may be compromised by human activity.

Carolina Oliveira de Santana et al.

Status: final response (author comments only)
Status: final response (author comments only)
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

Carolina Oliveira de Santana et al.

Data sets

Data from: Metagenomics of tidal zones of pristine mangrove sediments P. Spealman, C. Santana, D. Gresham, V. M. M. Melo, T. B. de Jesus, and F. A. Chinalia

Carolina Oliveira de Santana et al.


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Short summary
This study highlights the influence of tidal zonation on the prokaryotic sediment communities of a pristine mangrove forest. We observed that the variability of environmental factors between tidal zones results in significant differences in structure, diversity and function of prokaryotic populations. Surprisingly, we find the dynamic intertidal zone has the least biodiversity. This suggests that further work is needed in determining the role tidal microhabitat biodiversity has on mangroves.