Journal cover Journal topic
Biogeosciences An interactive open-access journal of the European Geosciences Union
Journal topic

Journal metrics

IF value: 3.480
IF3.480
IF 5-year value: 4.194
IF 5-year
4.194
CiteScore value: 6.7
CiteScore
6.7
SNIP value: 1.143
SNIP1.143
IPP value: 3.65
IPP3.65
SJR value: 1.761
SJR1.761
Scimago H <br class='widget-line-break'>index value: 118
Scimago H
index
118
h5-index value: 60
h5-index60
Preprints
https://doi.org/10.5194/bg-2020-282
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-2020-282
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

  21 Sep 2020

21 Sep 2020

Review status
This preprint is currently under review for the journal BG.

Technical Note: Inexpensive modification of Exetainers for the reliable storage of trace-level hydrogen and carbon monoxide gas samples

Philipp A. Nauer1, Eleonora Chiri2, Thanavit Jirapanjawat2, Chris Greening2, and Perran L. M. Cook1 Philipp A. Nauer et al.
  • 1School of Chemistry, Monash University, Clayton, VIC 3800, Australia
  • 2Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia

Abstract. Atmospheric trace gases such as dihydrogen (H2), carbon monoxide (CO) and methane (CH4) play important roles in microbial metabolism and biogeochemical cycles. Analysis of these gases at trace levels requires reliable storage of discrete samples of low volume. While commercial sampling vials such as Exetainers® have been tested for CH4 and other greenhouse gases, no information on reliable storage is available for H2 and CO. We show that vials sealed with butyl rubber stoppers are not suitable for storing H2 and CO due to release of these gases from rubber material. Treating butyl septa with NaOH reduced trace gas release, but contamination was still substantial, with H2 and CO concentrations in air samples increasing by a factor of 3 and 10 after 30 days of storage in conventional 12 mL Exetainers. Among the rubber materials tested, silicone showed the lowest potential for H2 and CO release. We thus propose to modify Exetainers by closing them with a silicone plug, and sealing them with a stainless steel bolt and O-ring for long-term storage. Such modified Exetainers exhibited stable concentrations of H2 and CH4 exceeding 60 days of storage at atmospheric and elevated (10 ppm) concentrations. The increase of CO was still measurable, but nine times lower than in conventional Exetainers with treated septa, and can be corrected for due to its linearity by storing a standard gas alongside the samples. The proposed modification is inexpensive, scalable and robust, and thus enables reliable storage of large numbers of low-volume gas samples from remote field locations.

Philipp A. Nauer et al.

Interactive discussion

Status: open (until 16 Nov 2020)
Status: open (until 16 Nov 2020)
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
[Subscribe to comment alert] Printer-friendly Version - Printer-friendly version Supplement - Supplement

Philipp A. Nauer et al.

Philipp A. Nauer et al.

Viewed

Total article views: 222 (including HTML, PDF, and XML)
HTML PDF XML Total Supplement BibTeX EndNote
187 32 3 222 10 2 2
  • HTML: 187
  • PDF: 32
  • XML: 3
  • Total: 222
  • Supplement: 10
  • BibTeX: 2
  • EndNote: 2
Views and downloads (calculated since 21 Sep 2020)
Cumulative views and downloads (calculated since 21 Sep 2020)

Viewed (geographical distribution)

Total article views: 96 (including HTML, PDF, and XML) Thereof 96 with geography defined and 0 with unknown origin.
Country # Views %
  • 1
1
 
 
 
 

Cited

Saved

No saved metrics found.

Discussed

No discussed metrics found.
Latest update: 25 Oct 2020
Publications Copernicus
Download
Short summary
Hydrogen (H2) and carbon monoxide (CO) are atmospheric trace gases cycled via microbial metabolisms. We observed strong H2 and CO contamination from rubber septa used to seal common gas-sample storage vials. Here we propose a simple and inexpensive modification of such vials to allow reliable storage of H2, CO and methane trace-gas samples for timescales of weeks to months, thus enabling extensive field campaigns to investigate H2 and CO biogeochemistry in remote areas.
Hydrogen (H2) and carbon monoxide (CO) are atmospheric trace gases cycled via microbial...
Citation
Altmetrics