<p>The Bohai Sea is a semi-closed marginal sea impacted by one of the most populated areas of China. The supply of nutrients, markedly that of reactive nitrogen, via fluvial and atmospheric transport has strongly increased in parallel with the growing population. It is therefore crucial to quantify the reactive nitrogen input to the BHS and to understand the processes and determine the quantities of nitrogen eliminated in and exported from the BHS. The nitrogen budget and in particular the internal sources and sinks of nitrate were constrained by using a mass-based and dual stable-isotope approach based on δ<sup>15</sup>N and δ<sup>18</sup>O of nitrate. Samples of water, suspended matter and sediments were taken in the BHS in spring (March and April) and summer (July and August) 2018. The Yellow River was sampled in May, July to November and Daliao River, Hai River, Luan River and Xiaoqing River were sampled in November of 2018. In addition to nutrient, particulate organic carbon and nitrogen concentrations, the dual isotopes of nitrate (δ<sup>15</sup>N and δ<sup>18</sup>O), δ<sup>15</sup>N of suspended matters and sediments were determined. Based on the available mass fluxes and isotope data an updated nitrogen budget is proposed. Compared to previous estimates, it is more complete and includes the impact of interior cycling (nitrification) on the nitrate pool. The main nitrogen sources are rivers contributing 17.5 %–20.6 % and the combined terrestrial runoff (including submarine discharge of nitrate with fresh ground water) accounting for 22.6 %–26.5 % of the nitrate input to the BHS while atmospheric input contributes only 6.3 %–7.4 % to total nitrate. An unusually active interior nitrogen cycling contributes 59.1 %–71.2 % to total nitrate via nitrification. Nitrogen is mainly trapped in the BHS and mainly removed by sedimentation (96.4 %–96.9 %) and only very little is exported to the YS (only 1.7 %–2.0 %). At present denitrification is only active in the sediments and removes 1.4 %–1.7 % of nitrate from the pool. A further eutrophication of the BHS could, however, induce water column hypoxia and denitrification as already observed – often seasonally off river mouths – in other marginal seas.</p>