<p>The deep soil, >1 meter, harbors a substantial share of the global microbial biomass. Currently, it is not known whether microbial activity several meters below the surface is fueled by recently fixed carbon or by old carbon that persisted in soil for several hundred years. Understanding the carbon source of microbial activity in deep soil is important to identify the drivers of biotic processes in the critical zone. Therefore, we explored carbon cycling in soils in three climate zones (arid, mediterranean, and humid) of the Coastal Cordillera of Chile down to a depth of six meters, using carbon isotopes. Specifically, we determined the <sup>13</sup>C : <sup>12</sup>C ratio (δ<sup>13</sup>C) of soil and roots, and the <sup>14</sup>C : <sup>12</sup>C ratio (Δ<sup>14</sup>C) of soil and CO<sub>2</sub> respired by microorganisms. We found that the Δ<sup>14</sup>C of the respired CO<sub>2</sub>-C was higher than of the soil organic carbon in all soils (except for two topsoils). Further, we found that the δ<sup>13</sup>C of the soil organic carbon changed only in the upper decimeters (by less than 6 ‰). Our results show that microbial activity several meters below the soil surface is mostly fueled by recently fixed carbon that is on average much younger than the total soil organic carbon present in the respective soil depth increments, in all three climate zones. Further, our results indicate that strong microbial decomposition of the soil organic matter only occurs in the upper decimeters of the soils, which is likely due to stabilization of organic carbon in the deep soil. In conclusion, our results demonstrate that microbial processes in the deep soil several meters below the surface are closely tied to primary production aboveground.</p>