07 Aug 2023
 | 07 Aug 2023
Status: a revised version of this preprint was accepted for the journal BG.

Soil priming effects and involved microbial community along salt gradients

Haoli Zhang, Doudou Chang, Zhifeng Zhu, Chunmei Meng, and Kaiyong Wang

Abstract. Soil salinity mediates microorganisms and soil process, like soil organic carbon (SOC) cycling. Yet, how soil salinity affects SOC mineralization via shaping bacterial communities diversity and composition remains elusive. Therefore, soils were sampled along a salt gradient (salinity at 0.25 %, 0.58 %, 0.75 %, 1.00 % and 2.64 %) and incubated for 90 days to investigate i) SOC mineralization (i.e. soil priming effects induced by cottonseed meal, as substrate) and ii) responsible bacteria community, by using high throughput sequencing and natural abundance 13C isotopes (to partition cottonseed meal derived CO2 and soil derived CO2. We observed negative priming effect during first 28 days of incubation but turned to positive priming effect after day 56. Negative priming at the early stage might be due to the preferential utilization of cottonseed meal. The followed positive priming decreased with the increase of salinity, which might be caused by the decreased alpha diversity of microbial community in soil with high salinity. Specifically, soil pH and EC along salinity gradient were the dominant variables modulating the structure of microbial community and consequently SOC priming (estimated by distance-based multivariate analysis and path analysis). By adopting O2PLS, priming effects were linked with specific microbial taxa, e.g., Proteobacteria (Luteimonas, Hoeflea and Stenotrophomonas) were the core microbial genus that attributed to the substrate induced priming effects. Here, we highlight that the increase of salinity reduced the diversity of microbial community and shifted dominant microorganisms that determined SOC priming effects, which provides a theoretical basis for understanding of SOC dynamics and microbial drivers under salinity gradient.

Haoli Zhang et al.

Status: final response (author comments only)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on bg-2023-114', Anonymous Referee #1, 09 Aug 2023
    • RC2: 'Reply on RC1', Mengjie An, 19 Aug 2023
      • AC1: 'Reply on RC6', Kaiyong Wang, 19 Sep 2023
    • RC4: 'Reply on RC1', Anonymous Referee #1, 10 Sep 2023
      • AC1: 'Reply on RC6', Kaiyong Wang, 19 Sep 2023
    • AC1: 'Reply on RC6', Kaiyong Wang, 19 Sep 2023
  • CC1: 'Comment on bg-2023-114', Shuangyi Li, 12 Aug 2023
    • RC3: 'Reply on CC1', Anonymous Referee #1, 19 Aug 2023
      • AC1: 'Reply on RC6', Kaiyong Wang, 19 Sep 2023
    • RC5: 'Reply on CC1', Anonymous Referee #1, 10 Sep 2023
      • RC6: 'Reply on RC5', Anonymous Referee #2, 18 Sep 2023
        • AC1: 'Reply on RC6', Kaiyong Wang, 19 Sep 2023
          • RC7: 'Reply on AC1', Anonymous Referee #2, 19 Sep 2023
            • AC1: 'Reply on RC6', Kaiyong Wang, 19 Sep 2023
  • RC8: 'Comment on bg-2023-114', Anonymous Referee #1, 19 Sep 2023
    • AC1: 'Reply on RC6', Kaiyong Wang, 19 Sep 2023

Haoli Zhang et al.

Haoli Zhang et al.


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Short summary
We conducted a 90 days of indoor incubation, and found soil microbial community was mainly controled by soil pH and EC. By O2PLS, we found Actinobacteria and Proteobacteria (Luteimonas, Hoeflea and Stenotrophomonas) dominant in these soils were the core microbial taxa that affecting the process of organic C mineralization. To clarify the priming effects and involved microbial groups would help us better understanding C sequestration potential and underlying mechanisms in saline soils.