Articles | Volume 10, issue 11
Biogeosciences, 10, 7631–7645, 2013
Biogeosciences, 10, 7631–7645, 2013

Research article 25 Nov 2013

Research article | 25 Nov 2013

Comparison of inorganic nitrogen uptake dynamics following snowmelt and at peak biomass in subalpine grasslands

N. Legay1, F. Grassein2, T. M. Robson3, E. Personeni4, M.-P. Bataillé4, S. Lavorel1, and J.-C. Clément1 N. Legay et al.
  • 1Laboratoire d'Ecologie Alpine, UMR CNRS 5553, Université Joseph Fourier, Grenoble, France
  • 2Institute of Plant Science, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland
  • 3Department of Biosciences, Plant Biology, P.O. Box 65, 00014 University of Helsinki, Finland
  • 4INRA, UMR950, EVA, Ecophysiologie Végétale, Agronomie et nutritions N, C, S, Université de Caen Basse-Normandie, Caen, France

Abstract. Subalpine grasslands are highly seasonal environments and likely subject to strong variability in nitrogen (N) dynamics. Plants and microbes typically compete for N acquisition during the growing season and particularly at plant peak biomass. During snowmelt, plants could potentially benefit from a decrease in competition by microbes, leading to greater plant N uptake associated with active growth and freeze-thaw cycles restricting microbial growth. In managed subalpine grasslands, we expect these interactions to be influenced by recent changes in agricultural land use, and associated modifications in plant and microbial communities. At several subalpine grasslands in the French Alps, we added pulses of 15N to the soil at the end of snowmelt, allowing us to compare the dynamics of inorganic N uptake in plants and microbes during this period with that previously reported at the peak biomass in July. In all grasslands, while specific shoot N translocation (per g of biomass) of dissolved inorganic nitrogen (DIN) was two to five times greater at snowmelt than at peak biomass, specific microbial DIN uptakes were similar between the two sampling dates. On an area basis, plant communities took more DIN than microbial communities at the end of snowmelt when aboveground plant biomasses were at least two times lower than at peak biomass. Consequently, inorganic N partitioning after snowmelt switches in favor of plant communities, allowing them to support their growing capacities at this period of the year. Seasonal differences in microbial and plant inorganic N-related dynamics were also affected by past (terraced vs. unterraced) rather than current (mown vs. unmown) land use. In terraced grasslands, microbial biomass N remained similar across seasons, whereas in unterraced grasslands, microbial biomass N was higher and microbial C : N lower at the end of snowmelt as compared to peak biomass. Further investigations on microbial community composition and their organic N uptake dynamics are required to better understand the decrease in microbial DIN uptake.

Final-revised paper