Articles | Volume 10, issue 12
Biogeosciences, 10, 8083–8091, 2013
Biogeosciences, 10, 8083–8091, 2013

Research article 10 Dec 2013

Research article | 10 Dec 2013

Spores of many common airborne fungi reveal no ice nucleation activity in oil immersion freezing experiments

B. G. Pummer1,5, L. Atanasova2, H. Bauer3,†, J. Bernardi4, I. S. Druzhinina2, J. Fröhlich-Nowoisky5, and H. Grothe1 B. G. Pummer et al.
  • 1Institute of Materials Chemistry, Vienna University of Technology, Austria
  • 2Research Area Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Austria
  • 3Institute of Chemical Technologies and Analytics, Vienna University of Technology, Austria
  • 4Service Center for Transmission Electron Microscopy, Vienna University of Technology, Austria
  • 5Department of Multiphase Chemistry, Max Planck Institute for Chemistry, Mainz, Germany
  • deceased: 31 October 2012

Abstract. Fungal spores are ubiquitous biological aerosols, which are considered to act as ice nuclei. In this study the ice nucleation (IN) activity of spores harvested from 29 fungal strains belonging to 21 different species was tested in the immersion freezing mode by microscopic observation of water-in-oil emulsions. Spores of 8 of these strains were also investigated in a microdroplet freezing array instrument. The focus was laid on species of economical, ecological or sanitary significance. Besides common molds (Ascomycota), some representatives of the widespread group of mushrooms (Basidiomycota) were also investigated.

Fusarium avenaceum was the only sample showing IN activity at relatively high temperatures (about 264 K), while the other investigated fungal spores showed no freezing above 248 K. Many of the samples indeed froze at homogeneous ice nucleation temperatures (about 237 K). In combination with other studies, this suggests that only a limited number of species may act as atmospheric ice nuclei. This would be analogous to what is already known for the bacterial ice nuclei.

Apart from that, we selected a set of fungal strains from different sites and exposed them to occasional freezing stress during their cultivation. This was in order to test if the exposure to a cold environment encourages the expression of ice nuclei during growth as a way of adaptation. Although the total protein expression was altered by this treatment, it had no significant impact on the IN activity.

Final-revised paper