Articles | Volume 11, issue 16
Biogeosciences, 11, 4381–4391, 2014
Biogeosciences, 11, 4381–4391, 2014

Research article 20 Aug 2014

Research article | 20 Aug 2014

Field 13CO2 pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a Kobresia meadow

J. Zou1,3, L. Zhao1, S. Xu1, X. Xu2, D. Chen1, Q. Li1, N. Zhao1, C. Luo1, and X. Zhao1 J. Zou et al.
  • 1Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, China
  • 2Key Laboratory of Ecosystem Network Observation and Modelling, Institute of Geographical Sciences & Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China
  • 3University of Chinese Academy of Sciences, Beijing 100049, China

Abstract. Livestock exclosure has been widely used as an approach for grassland restoration. However, the effects of exclosures on grasslands are controversial and can depend on many factors, such as the grassland ecosystem types, evolutionary history and so on. In this study, we conduct field experiments to investigate the variations of the ecosystem function in response to livestock exclosure in a Kobresia humilis meadow with 6 years of grazing exclosure on the Qinghai–Tibetan Plateau. We focused on two ecosystem functions: plant community structure and ecosystem carbon cycling. The plant aboveground productivity, plant diversity and the composition of plant functional groups of the meadow were addressed as the indicators of the plant community structure. The 13C isotope pulse labeling technique was applied to evaluate the alterations of ecosystem carbon cycling during a short term. The results showed that the plant community structure was changed after being fenced in for 6 years, with significantly decreased aboveground productivity, species loss and varied composition of the four plant functional groups (grasses, sedges, legumes and forbs). Using the pulse labeling technique, we found a lower cycling rate of 13C in the plant–soil system of the fenced plots compared with the grazed sites during the first 24 h after labeling. A higher proportion of recovered 13C in the plant–soil system migrated into the soil as root exudates immediately after labeling at both fenced and control grazed sites, with a significantly lower proportion in the fenced site, coinciding with the lower proportion of 13C lost from soil respiration. Thirty-two days after labeling, 37% of the recovered 13C remained in the soil of the fenced plots, with significant differences compared to in the grazed plots (47%). In addition, less 13C (5 vs. 7%) was lost by soil respiration in the fenced plots during the chase period of 32 days. Overall, our study suggests that livestock exclosures have negative effects on the plant community structure and partitioning patterns of the photoassimilated carbon in the Kobresia meadow, and the effects on photoassimilated carbon cycling are likely to result from the variations of community structures in the ecosystem.

Final-revised paper