Articles | Volume 12, issue 13
Biogeosciences, 12, 4099–4112, 2015

Special issue: Biogeochemical and biological response to a diazotroph bloom...

Biogeosciences, 12, 4099–4112, 2015

Research article 07 Jul 2015

Research article | 07 Jul 2015

Dinitrogen fixation and dissolved organic nitrogen fueled primary production and particulate export during the VAHINE mesocosm experiment (New Caledonia lagoon)

H. Berthelot1, T. Moutin1, S. L'Helguen2, K. Leblanc1, S. Hélias1, O. Grosso1, N. Leblond3, B. Charrièrea,1, and S. Bonnet4,1 H. Berthelot et al.
  • 1Aix Marseille Université, CNRS/INSU, Université de Toulon, IRD, Mediterranean Institute of Oceanography (MIO) UM110, 13288, Marseille, France
  • 2Université de Brest, CNRS/IRD, UMR6539, Laboratoire des Sciences de l'Environnement Marin, OSU-IUEM, 29280 Plouzané, France
  • 3Observatoire Océanologique de Villefranche, Laboratoire d'Océanographie de Villefranche, UMR 7093, Villefranche-sur-mer, France
  • 4Mediterranean Institute of Oceanography (MIO) – IRD/CNRS/Aix-Marseille University IRD Noumea, 101 Promenade R. Laroque, BPA5, 98848, Noumea CEDEX, New Caledonia
  • anow at: Centre de Formation et de Recherche sur les Environnements Méditerranéens CNRS UMR5110, Université de Perpignan Via Domitia, 66860 Perpignan, France

Abstract. In the oligotrophic ocean characterized by nitrate (NO3) depletion in surface waters, dinitrogen (N2) fixation and dissolved organic nitrogen (DON) can represent significant nitrogen (N) sources for the ecosystem. In this study, we deployed large in situ mesocosms in New Caledonia in order to investigate (1) the contribution of N2 fixation and DON use to primary production (PP) and particle export and (2) the fate of the freshly produced particulate organic N (PON), i.e., whether it is preferentially accumulated and recycled in the water column or exported out of the system. The mesocosms were fertilized with phosphate (PO43-) in order to prevent phosphorus (P) limitation and promote N2 fixation. The diazotrophic community was dominated by diatom–diazotroph associations (DDAs) during the first part of the experiment for 10 days (P1) followed by the unicellular N2-fixing cyanobacteria UCYN-C for the last 9 days (P2) of the experiment. N2 fixation rates averaged 9.8 ± 4.0 and 27.7 ± 8.6 nmol L−1 d−1 during P1 and P2, respectively. NO3 concentrations (< 0.04 μmol L−1) in the mesocosms were a negligible source of N, indicating that N2 fixation was the main driver of new production throughout the experiment. The contribution of N2 fixation to PP was not significantly different (p > 0.05) during P1 (9.0 ± 3.3 %) and P2 (12.6 ± 6.1 %). However, the e ratio that quantifies the efficiency of a system to export particulate organic carbon (POCexport) compared to PP (e ratio = POCexport/PP) was significantly higher (p < 0.05) during P2 (39.7 ± 24.9 %) than during P1 (23.9 ± 20.2 %), indicating that the production sustained by UCYN-C was more efficient at promoting C export than the production sustained by DDAs. During P1, PON was stable and the total amount of N provided by N2 fixation (0.10 ± 0.02 μmol L−1) was not significantly different (p > 0.05) from the total amount of PON exported (0.10 ± 0.04 μmol L−1), suggesting a rapid and probably direct export of the recently fixed N2 by the DDAs. During P2, both PON concentrations and PON export increased in the mesocosms by a factor 1.5–2. Unlike in P1, this PON production was not totally explained by the new N provided by N2 fixation. The use of DON, whose concentrations decreased significantly (p < 0.05) from 5.3 ± 0.5 μmol L−1 to 4.4 ± 0.5 μmol L−1, appeared to be the missing N source. The DON consumption (~ 0.9 μmol L−1) during P2 is higher than the total amount of new N brought by N2 fixation (~ 0.25 μmol L−1) during the same period. These results suggest that while DDAs mainly rely on N2 fixation for their N requirements, both N2 fixation and DON can be significant N sources for primary production and particulate export following UCYN-C blooms in the New Caledonia lagoon and by extension in the N-limited oceans where similar events are likely to occur.

Final-revised paper