Articles | Volume 6, issue 6
Biogeosciences, 6, 1127–1138, 2009
Biogeosciences, 6, 1127–1138, 2009

  29 Jun 2009

29 Jun 2009

Wetland restoration and methanogenesis: the activity of microbial populations and competition for substrates at different temperatures

V. Jerman1,2, M. Metje1, I. Mandić-Mulec2, and P. Frenzel1 V. Jerman et al.
  • 1Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Str., 35043 Marburg, Germany
  • 2University of Ljubljana, Biotechnical Faculty, Department of Food Science and Technology, Chair of Microbiology, Večna pot 111, 1000 Ljubljana, Slovenia

Abstract. Ljubljana marsh in Slovenia is a 16 000 ha area of partly drained fen, intended to be flooded to restore its ecological functions. The resultant water-logging may create anoxic conditions, eventually stimulating production and emission of methane, the most important greenhouse gas next to carbon dioxide. We examined the upper layer (~30 cm) of Ljubljana marsh soil for microbial processes that would predominate in water-saturated conditions, focusing on the potential for iron reduction, carbon mineralization (CO2 and CH4 production), and methane emission. Methane emission from water-saturated microcosms was near minimum detectable levels even after extended periods of flooding (>5 months). Methane production in anoxic soil slurries started only after a lag period of 84 d at 15°C and a minimum of 7 d at 37°C, the optimum temperature for methanogenesis. This lag was inversely related to iron reduction, which suggested that iron reduction out-competed methanogenesis for electron donors, such as H2 and acetate. Methane production was observed only in samples incubated at 14–38°C. At the beginning of methanogenesis, acetoclastic methanogenesis dominated. In accordance with the preferred substrate, most (91%) mcrA (encoding the methyl coenzyme-M reductase, a key gene in methanogenesis) clone sequences could be affiliated to the acetoclastic genus Methanosarcina. No methanogens were detected in the original soil. However, a diverse community of iron-reducing Geobacteraceae was found. Our results suggest that methane emission can remain transient and low if water-table fluctuations allow re-oxidation of ferrous iron, sustaining iron reduction as the most important process in terminal carbon mineralization.

Final-revised paper