Articles | Volume 8, issue 12
Biogeosciences, 8, 3747–3759, 2011

Special issue: Molecular biogeochemical provinces in the eastern Atlantic...

Biogeosciences, 8, 3747–3759, 2011

Research article 20 Dec 2011

Research article | 20 Dec 2011

Biogeochemical controls on the bacterial populations in the eastern Atlantic Ocean

S. B. Neogi1,2, B. P. Koch3,4, P. Schmitt-Kopplin5, C. Pohl6, G. Kattner3, S. Yamasaki1, and R. J. Lara7,8 S. B. Neogi et al.
  • 1Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan
  • 2International Centre for Diarrhoeal Disease Research, Bangladesh, 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka 1212, Bangladesh
  • 3Alfred Wegener Institute for Polar and Marine Research, Ecological Chemistry, Am Handelshafen 12, 27570 Bremerhaven, Germany
  • 4University of Applied Sciences, 27568 Bremerhaven, Germany
  • 5Research Unit BioGeoChemistry and Analytics, Helmholtz Zentrum München, Ingoldstädter Landstr. 1, 85764 Neuherberg, Germany
  • 6Leibniz Institute for Baltic Sea Research, Seestr. 15, 18119 Warnemünde, Germany
  • 7Argentine Institute of Oceanography, 8000 Bahía Blanca, Argentina
  • 8Leibniz Centre for Tropical Marine Ecology GmbH, Fahrenheitstr. 6, 28359 Bremen, Germany

Abstract. Little is known about bacterial dynamics in the oligotrophic ocean, particularly about cultivable bacteria. We examined the abundance of total and cultivable bacteria in relation to changes in biogeochemical conditions in the eastern Atlantic Ocean with special regard to Vibrio spp., a group of bacteria that can cause diseases in human and aquatic organisms. Surface, deep water and plankton (<20 μm, 20–55 μm and >55 μm) samples were collected between 50° N and 24° S. Chlorophyll-a was very low (<0.3 μg l−1) in most areas of the nutrient-poor Atlantic, except at a few locations near upwelling regions. In surface water, dissolved organic carbon (DOC) and nitrogen (DON) concentrations were 64–95 μM C and 2–10 μM N accounting for ≥90 % and ≥76 % of total organic C and N, respectively. DOC and DON gradually decreased to ~45 μM C and <5 μM N in the bottom water. In the surface layer, culture independent total bacteria and other prokaryotes represented by 4´-6-diamidino-2-phenylindole (DAPI) counts, ranged mostly between 107 and 108 cells l−1, while cultivable bacterial counts (CBC) and Vibrio spp. were found at concentrations of 104–107 and 102–105 colony forming units (CFU) l−1, respectively. Most bacteria (>99 %) were found in the nanoplankton fraction (<20 μm), however, bacterial abundance did not correlate with suspended particulates (chlorophyll-a, particulate organic C [POC] and N [PON]). Instead, we found a highly significant correlation between bacterial abundance and temperature (p < 0.001) and a significant correlation with DOC and DON (p < 0.005 and <0.01, respectively). In comparison to CBC and DAPI-stained prokaryotes, cultivable Vibrio showed a stronger and highly significant correlation with DOC and DON (p < 0.0005 and p < 0.005, respectively). In cold waters of the mesopelagic and abyssal zones, CBC was 50 to 100-times lower than in the surface layer; however, cultivable Vibrio spp. could be isolated from the bathypelagic zone and even near the seafloor (average ~10 CFU l−1). The depth-wise decrease in CBC and Vibrio coincided with the decrease in both DOC and POC. Our study indicates that Vibrio and other bacteria may largely depend on dissolved organic matter to survive in nutrient-poor oceanic habitats.

Final-revised paper