Articles | Volume 11, issue 1
Biogeosciences, 11, 185–194, 2014
Biogeosciences, 11, 185–194, 2014

Research article 14 Jan 2014

Research article | 14 Jan 2014

Acquisition of intact polar lipids from the prymnesiophyte Phaeocystis globosa by its lytic virus PgV-07T

D. S. Maat1,**, N. J. Bale1,**, E. C. Hopmans1, A.-C. Baudoux*, J. S. Sinninghe Damsté1, S. Schouten1, and C. P. D. Brussaard1,2 D. S. Maat et al.
  • 1Departments of Biological Oceanography and Marine Organic Biogeochemistry, Royal Netherlands Institute for Sea Research, P.O. Box 59, 1790 AB Den Burg, the Netherlands
  • 2Aquatic Microbiology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, P.O. Box 94248, 1090 GE Amsterdam, the Netherlands
  • *present address: CNRS and UPMC Univ Paris 06, UMR7144, Station Biologique de Roscoff, Place Georges Teissier, 29680 Roscoff, France
  • **These authors have contributed equally to the manuscript.

Abstract. Recent studies showed changes in phytoplankton lipid composition during viral infection and have indicated roles for specific lipids in the mechanisms of algal virus-host interaction. To investigate the generality of these findings and obtain a better understanding of the allocation of specific lipids to viruses, we studied the intact polar lipid (IPL) composition of virally infected and non-infected cultures of the prymnesiophyte Phaeocystis globosa G(A) and its lytic virus PgV-07T. The P. globosa IPL composition was relatively stable over a diel cycle and not strongly affected by viral infection. Glycolipids, phospholipids and betaine lipids were present in both the host and virus, although specific groups such as the diacylglyceryl-hydroxymethyltrimethyl-β-alanines and the sulfoquinovosyldiacylglycerols, were present in a lower proportion or were not detected in the virus. Viral glycosphingolipids (vGSLs), which have been shown to play a role in the infection strategy of the virus EhV-86, infecting the prymnesiophyte Emiliania huxleyi CCMP374, were not encountered. Our results show that the involvement of lipids in virus–algal host interactions can be very different amongst virus–algal host systems.

Final-revised paper