Articles | Volume 12, issue 22
https://doi.org/10.5194/bg-12-6781-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/bg-12-6781-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
Research article
| 
26 Nov 2015
Research article |
| 26 Nov 2015
Chlorophyll a-specific Δ14C, δ13C and δ15N values in stream periphyton: implications for aquatic food web studies
N. F. Ishikawa
CORRESPONDING AUTHOR
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
M. Yamane
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
Atmosphere and Ocean Research Institute, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan
H. Suga
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
N. O. Ogawa
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
Y. Yokoyama
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
Atmosphere and Ocean Research Institute, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan
N. Ohkouchi
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan
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Cited
29 citations as recorded by crossref.
- Dating Lake Sediments Using Compound‐Specific 14C Analysis of C16 Fatty Acid: A Case Study From the Mount Fuji Volcanic Region, Japan S. Yamamoto et al. https://doi.org/10.1029/2020GC009544
- Organic matter sources and flows in tundra wetland food webs S. Plesh et al. https://doi.org/10.1371/journal.pone.0286368
- Eruptive history of Mt. Fuji over the past 8000 years based on integrated records of lacustrine and terrestrial tephra sequences and radiocarbon dating S. Yamamoto et al. https://doi.org/10.1016/j.qsa.2023.100091
- Periphyton C and N stable isotopes detect agricultural stressors in low-order streams S. Whorley & J. Wehr https://doi.org/10.1086/719187
- Hydrologically driven apparent photosynthetic aging effect in karst aquatic ecosystems and its implications for aquatic carbon cycling D. Li et al. https://doi.org/10.1016/j.jhydrol.2026.135753
- Origin of Deep Methane from Active Faults along the Itoigawa–Shizuoka Tectonic Line between the Eurasian and North American Plates: 13C/12C and 14C/12C Methane Profiles from a Pull-Apart Basin at Lake Suwa A. Urai et al. https://doi.org/10.1021/acsearthspacechem.1c00392
- Forest cover controls the nitrogen and carbon stable isotopes of rivers F. Machado-Silva et al. https://doi.org/10.1016/j.scitotenv.2021.152784
- Influence of land use and lithology on sources and ages of nutritional resources for stream macroinvertebrates: a multi-isotopic approach A. Bellamy et al. https://doi.org/10.1007/s00027-017-0542-3
- Origin of Carbon and Essential Fatty Acids in Higher Trophic Level Fish in Headwater Stream Food Webs M. Fujibayashi et al. https://doi.org/10.3390/biom9090487
- Assessing the applicability of sequential eye lens stable isotope analysis for reconstructing dietary histories in amphibians A. Shiga et al. https://doi.org/10.1002/lom3.10689
- Chromatographic isolation for performing enantiomer-specific radiocarbon analysis (ESRA): first method development for individual L- and D-amino acids T. Blattmann et al. https://doi.org/10.1186/s40645-025-00764-w
- Carbon Uptake in Surface Water Food Webs Fed by Palaeogroundwater D. Mazumder et al. https://doi.org/10.1029/2018JG004925
- Radiocarbon in ecology: Insights and perspectives from aquatic and terrestrial studies T. Larsen et al. https://doi.org/10.1111/2041-210X.12851
- High-resolution radiocarbon, cosmogenic radionuclides and Uranium series dating for paleoclimate and paleoenvironment studies Y. Yokoyama https://doi.org/10.4116/jaqua.58.265
- Multiple controls on carbon dynamics in mixed karst and non-karst mountainous rivers, Southwest China, revealed by carbon isotopes (δ13C and Δ14C) S. Chen et al. https://doi.org/10.1016/j.scitotenv.2021.148347
- Discharge determines production of, decomposition of and quality changes in dissolved organic carbon in pre-dams of drinking water reservoirs K. Morling et al. https://doi.org/10.1016/j.scitotenv.2016.10.192
- Chlorophyll nitrogen isotope values track shifts between cyanobacteria and eukaryotic algae in a natural phytoplankton community in Lake Erie J. Kharbush et al. https://doi.org/10.1016/j.orggeochem.2018.12.006
- Permafrost Hydrology Drives the Assimilation of Old Carbon by Stream Food Webs in the Arctic J. O’Donnell et al. https://doi.org/10.1007/s10021-019-00413-6
- “Old” carbon entering the South China Sea from the carbonate-rich Pearl River Basin: Coupled action of carbonate weathering and aquatic photosynthesis Z. Liu et al. https://doi.org/10.1016/j.apgeochem.2016.12.014
- Nutritional support of inland aquatic food webs by aged carbon and organic matter A. Bellamy & J. Bauer https://doi.org/10.1002/lol2.10044
- Strong correspondence between nitrogen isotope composition of foliage and chlorin across a rainfall gradient: implications for paleo-reconstruction of the nitrogen cycle S. Goulden et al. https://doi.org/10.5194/bg-16-3869-2019
- Incorporation of local dissolved organic carbon into floodplain aquatic ecosystems N. Saintilan et al. https://doi.org/10.1007/s10452-021-09860-7
- Combined use of radiocarbon and stable carbon isotopes for the source mixing model in a stream food web N. Ishikawa et al. https://doi.org/10.1002/lno.11541
- Preference for fish in a Neolithic hunter-gatherer community of the upper Tigris, elucidated by amino acid δ15N analysis Y. Itahashi et al. https://doi.org/10.1016/j.jas.2017.05.001
- Working standards and analytical framework for CSIA: isotopic composition (δ13C, δ15N, and δ34S) of 21 amino acids and 7 nucleobases N. Ogawa et al. https://doi.org/10.1186/s40645-025-00782-8
- Terrestrial‐aquatic linkage in stream food webs along a forest chronosequence: multi‐isotopic evidence N. Ishikawa et al. https://doi.org/10.1890/15-1133.1
- Biogeochemical tools for characterizing organic carbon in inland aquatic ecosystems S. McCallister et al. https://doi.org/10.1002/lol2.10097
- Bomb-radiocarbon signal suggests that soil carbon contributes to chlorophyll a in archival oak leaves N. Ishikawa et al. https://doi.org/10.5194/bg-23-3855-2026
- Complementary information from fatty acid and nutrient stoichiometry data improve stream food web analyses M. Torres-Ruiz & J. Wehr https://doi.org/10.1007/s10750-019-04126-8
29 citations as recorded by crossref.
- Dating Lake Sediments Using Compound‐Specific 14C Analysis of C16 Fatty Acid: A Case Study From the Mount Fuji Volcanic Region, Japan S. Yamamoto et al. https://doi.org/10.1029/2020GC009544
- Organic matter sources and flows in tundra wetland food webs S. Plesh et al. https://doi.org/10.1371/journal.pone.0286368
- Eruptive history of Mt. Fuji over the past 8000 years based on integrated records of lacustrine and terrestrial tephra sequences and radiocarbon dating S. Yamamoto et al. https://doi.org/10.1016/j.qsa.2023.100091
- Periphyton C and N stable isotopes detect agricultural stressors in low-order streams S. Whorley & J. Wehr https://doi.org/10.1086/719187
- Hydrologically driven apparent photosynthetic aging effect in karst aquatic ecosystems and its implications for aquatic carbon cycling D. Li et al. https://doi.org/10.1016/j.jhydrol.2026.135753
- Origin of Deep Methane from Active Faults along the Itoigawa–Shizuoka Tectonic Line between the Eurasian and North American Plates: 13C/12C and 14C/12C Methane Profiles from a Pull-Apart Basin at Lake Suwa A. Urai et al. https://doi.org/10.1021/acsearthspacechem.1c00392
- Forest cover controls the nitrogen and carbon stable isotopes of rivers F. Machado-Silva et al. https://doi.org/10.1016/j.scitotenv.2021.152784
- Influence of land use and lithology on sources and ages of nutritional resources for stream macroinvertebrates: a multi-isotopic approach A. Bellamy et al. https://doi.org/10.1007/s00027-017-0542-3
- Origin of Carbon and Essential Fatty Acids in Higher Trophic Level Fish in Headwater Stream Food Webs M. Fujibayashi et al. https://doi.org/10.3390/biom9090487
- Assessing the applicability of sequential eye lens stable isotope analysis for reconstructing dietary histories in amphibians A. Shiga et al. https://doi.org/10.1002/lom3.10689
- Chromatographic isolation for performing enantiomer-specific radiocarbon analysis (ESRA): first method development for individual L- and D-amino acids T. Blattmann et al. https://doi.org/10.1186/s40645-025-00764-w
- Carbon Uptake in Surface Water Food Webs Fed by Palaeogroundwater D. Mazumder et al. https://doi.org/10.1029/2018JG004925
- Radiocarbon in ecology: Insights and perspectives from aquatic and terrestrial studies T. Larsen et al. https://doi.org/10.1111/2041-210X.12851
- High-resolution radiocarbon, cosmogenic radionuclides and Uranium series dating for paleoclimate and paleoenvironment studies Y. Yokoyama https://doi.org/10.4116/jaqua.58.265
- Multiple controls on carbon dynamics in mixed karst and non-karst mountainous rivers, Southwest China, revealed by carbon isotopes (δ13C and Δ14C) S. Chen et al. https://doi.org/10.1016/j.scitotenv.2021.148347
- Discharge determines production of, decomposition of and quality changes in dissolved organic carbon in pre-dams of drinking water reservoirs K. Morling et al. https://doi.org/10.1016/j.scitotenv.2016.10.192
- Chlorophyll nitrogen isotope values track shifts between cyanobacteria and eukaryotic algae in a natural phytoplankton community in Lake Erie J. Kharbush et al. https://doi.org/10.1016/j.orggeochem.2018.12.006
- Permafrost Hydrology Drives the Assimilation of Old Carbon by Stream Food Webs in the Arctic J. O’Donnell et al. https://doi.org/10.1007/s10021-019-00413-6
- “Old” carbon entering the South China Sea from the carbonate-rich Pearl River Basin: Coupled action of carbonate weathering and aquatic photosynthesis Z. Liu et al. https://doi.org/10.1016/j.apgeochem.2016.12.014
- Nutritional support of inland aquatic food webs by aged carbon and organic matter A. Bellamy & J. Bauer https://doi.org/10.1002/lol2.10044
- Strong correspondence between nitrogen isotope composition of foliage and chlorin across a rainfall gradient: implications for paleo-reconstruction of the nitrogen cycle S. Goulden et al. https://doi.org/10.5194/bg-16-3869-2019
- Incorporation of local dissolved organic carbon into floodplain aquatic ecosystems N. Saintilan et al. https://doi.org/10.1007/s10452-021-09860-7
- Combined use of radiocarbon and stable carbon isotopes for the source mixing model in a stream food web N. Ishikawa et al. https://doi.org/10.1002/lno.11541
- Preference for fish in a Neolithic hunter-gatherer community of the upper Tigris, elucidated by amino acid δ15N analysis Y. Itahashi et al. https://doi.org/10.1016/j.jas.2017.05.001
- Working standards and analytical framework for CSIA: isotopic composition (δ13C, δ15N, and δ34S) of 21 amino acids and 7 nucleobases N. Ogawa et al. https://doi.org/10.1186/s40645-025-00782-8
- Terrestrial‐aquatic linkage in stream food webs along a forest chronosequence: multi‐isotopic evidence N. Ishikawa et al. https://doi.org/10.1890/15-1133.1
- Biogeochemical tools for characterizing organic carbon in inland aquatic ecosystems S. McCallister et al. https://doi.org/10.1002/lol2.10097
- Bomb-radiocarbon signal suggests that soil carbon contributes to chlorophyll a in archival oak leaves N. Ishikawa et al. https://doi.org/10.5194/bg-23-3855-2026
- Complementary information from fatty acid and nutrient stoichiometry data improve stream food web analyses M. Torres-Ruiz & J. Wehr https://doi.org/10.1007/s10750-019-04126-8
Saved (final revised paper)
Latest update: 06 Aug 2026
Short summary
We determined the isotopic composition of chlorophyll a in periphytic algae attached to a streambed substrate (periphyton). Although the bulk isotopic composition of periphyton is recognised as a surrogate for the photosynthetic algal community, our results indicate that the bulk periphyton matrix at the study site consists of 89 to 95% algal carbon and 5 to 11% terrestrial organic carbon. We show that the chlorophyll a isotopic composition is a useful tracer for the aquatic food web studies.
We determined the isotopic composition of chlorophyll a in periphytic algae attached to a...
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