Articles | Volume 12, issue 3
Biogeosciences, 12, 863–873, 2015
Biogeosciences, 12, 863–873, 2015

Research article 12 Feb 2015

Research article | 12 Feb 2015

New highlights of phytolith structure and occluded carbon location: 3-D X-ray microscopy and NanoSIMS results

A. Alexandre1, I. Basile-Doelsch1, T. Delhaye2, D. Borshneck1, J. C. Mazur1, P. Reyerson3, and G. M. Santos4 A. Alexandre et al.
  • 1Centre Européen de Recherche et d'Enseignement des Géosciences de l'Environnement (UMR 7330), CNRS, Aix-Marseille Université, Europôle méditerranéen de l'Arbois BP 80, 13545 Aix en Provence CEDEX 04, France
  • 2Plateforme NanoSIMS, OSUR, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes CEDEX, France
  • 3Department of Geography, University of Wisconsin-Madison, 550 North Park Street, Madison, WI 53706, USA
  • 4Department of Earth System Science, University of California, Irvine, B321 Croul Hall, Irvine, CA 92697-3100, USA

Abstract. Phytoliths contain occluded organic compounds called phytC. Recently, phytC content, nature, origin, paleoenvironmental meaning and impact in the global C cycle have been the subject of increasing debate. Inconsistencies were fed by the scarcity of in situ characterizations of phytC in phytoliths. Here we reconstructed at high spatial resolution the 3-D structure of harvested grass short cell (GSC) phytoliths using 3-D X-ray microscopy. While this technique has been widely used for 3-D reconstruction of biological systems it has never been applied in high-resolution mode to silica particles. Simultaneously, we investigated the location of phytC using nanoscale secondary ion mass spectrometry (NanoSIMS). Our data evidenced that the silica structure contains micrometric internal cavities. These internal cavities were sometimes observed isolated from the outside. Their opening may be an original feature or may result from a beginning of dissolution of silica during the chemical extraction procedure, mimicking the progressive dissolution process that can happen in natural environments. The phytC that may originally occupy the cavities is thus susceptible to rapid oxidation. It was not detected by the NanoSIMS technique. However, another pool of phytC, continuously distributed in and protected by the silica structure, was observed. Its N/C ratio (0.27) is in agreement with the presence of amino acids. These findings constitute a basis to further characterize the origin, occlusion process, nature and accessibility of phytC, as a prerequisite for assessing its significance in the global C cycle.

Short summary
Phytoliths contain occluded organic compounds called phytC. The nature and location of phytC in biogenic silica structures is poorly understood. Here, we reconstructed the 3-D structure of phytoliths using 3-D Xray microscopy. We further evidenced a pool of phytC, continuously distributed in the silica structure, using nanoscale secondary ion mass spectrometry (NanoSIMS). Our findings allowed the re-evaluation of previous suggestions regarding phytC quantification and environmental meaning.
Final-revised paper