Articles | Volume 13, issue 6
https://doi.org/10.5194/bg-13-1821-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/bg-13-1821-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
Application of the 15N gas-flux method for measuring in situ N2 and N2O fluxes due to denitrification in natural and semi-natural terrestrial ecosystems and comparison with the acetylene inhibition technique
Fotis Sgouridis
CORRESPONDING AUTHOR
School of Physical and Geographical Sciences, Keele University,
Staffordshire, UK
now at: School of Geographical Sciences, University of Bristol, UK
Andrew Stott
NERC Life Sciences Mass Spectrometry Facility, Centre for
Ecology & Hydrology, Lancaster Environment Centre, Lancaster, UK
Sami Ullah
School of Physical and Geographical Sciences, Keele University,
Staffordshire, UK
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Cited
36 citations as recorded by crossref.
- Determining N2O and N2 fluxes in relation to winter wheat and sugar beet growth and development using the improved 15N gas flux method on the field scale J. Eckei et al. 10.1007/s00374-024-01806-z
- In situ 15N labeling reveals high soil N2 emission during anaerobic soil disinfestation period in a greenhouse vegetable production system X. Li et al. 10.1007/s11104-024-07014-w
- Looking back to look ahead: a vision for soil denitrification research M. Almaraz et al. 10.1002/ecy.2917
- Effects of nitrate and water content on acetylene inhibition technique bias when analysing soil denitrification rates under an aerobic atmosphere H. Yuan et al. 10.1016/j.geoderma.2018.07.039
- Measuring denitrification and the N2O:(N2O + N2) emission ratio from terrestrial soils J. Friedl et al. 10.1016/j.cosust.2020.08.006
- A global synthesis of soil denitrification: Driving factors and mitigation strategies B. Pan et al. 10.1016/j.agee.2021.107850
- Global analysis of agricultural soil denitrification in response to fertilizer nitrogen J. Wang et al. 10.1016/j.scitotenv.2017.10.229
- Microbial processes responsible for soil N2O production in a tropical rainforest, illustrated using an in situ 15N labeling approach W. Zhou et al. 10.1016/j.catena.2021.105214
- Improvement of the 15N gas flux method for in situ measurement of soil denitrification and its product stoichiometry R. Well et al. 10.1002/rcm.8363
- Characteristics of N2 and N2O Fluxes from a Cultivated Black Soil: A Case Study through In Situ Measurement Using the 15N Gas Flux Method Z. Pan et al. 10.3390/agriculture12101664
- Biological nitrogen fixation in peatlands: Comparison between acetylene reduction assay and 15N2 assimilation methods E. Saiz et al. 10.1016/j.soilbio.2019.01.011
- Rain and wind affect chamber measurements M. Maier et al. 10.1016/j.agrformet.2019.107754
- Towards enhanced sensitivity of the 15N gas flux method for quantifying denitrification in soil G. Micucci et al. 10.1016/j.soilbio.2024.109421
- Evaluation of denitrification and decomposition from three biogeochemical models using laboratory measurements of N<sub>2</sub>, N<sub>2</sub>O and CO<sub>2</sub> B. Grosz et al. 10.5194/bg-18-5681-2021
- Mobile continuous-flow isotope-ratio mass spectrometer system for automated measurements of N2 and N2O fluxes in fertilized cropping systems D. Warner et al. 10.1038/s41598-019-47451-7
- Soil Greenhouse Gas Fluxes, Environmental Controls, and the Partitioning of N2O Sources in UK Natural and Seminatural Land Use Types F. Sgouridis & S. Ullah 10.1002/2017JG003783
- Chronic atmospheric reactive N deposition has breached the N sink capacity of a northern ombrotrophic peatbog increasing the gaseous and fluvial N losses F. Sgouridis et al. 10.1016/j.scitotenv.2021.147552
- Biodegradation of biodiesel and toluene under nitrate-reducing conditions and the impact on bacterial community structure H. Ribeiro et al. 10.1007/s11368-018-2079-z
- Isotopomeric Peak Assignment for N2O in Cross-Labeling Experiments by Fiber-Enhanced Raman Multigas Spectroscopy A. Blohm et al. 10.1021/acs.analchem.3c04236
- Underestimation of denitrification rates from field application of the <sup>15</sup>N gas flux method and its correction by gas diffusion modelling R. Well et al. 10.5194/bg-16-2233-2019
- Application methods of tracers for N2O source determination lead to inhomogeneous distribution in field plots J. Berendt et al. 10.1002/ansa.202000100
- Spatial Variations of Soil N2 and N2O Emissions from a Temperate Forest: Quantified by the In Situ 15N Labeling Method D. Xi et al. 10.3390/f13091347
- Rapid Conversion of Added Nitrate to Nitrous Oxide and Dinitrogen in Northern Forest Soil M. Kulkarni et al. 10.1080/01490451.2016.1238981
- Effects of long-term increased N deposition on tropical montane forest soil N2 and N2O emissions W. Tang et al. 10.1016/j.soilbio.2018.08.027
- Quantifying in situ N2 fluxes from an intensively managed calcareous soil using the 15N gas-flux method Y. LIU et al. 10.1016/j.jia.2022.07.016
- Stimulation of soil gross nitrogen transformations and nitrous oxide emission under Free air CO2 enrichment in a mature temperate oak forest at BIFoR-FACE F. Sgouridis et al. 10.1016/j.soilbio.2023.109072
- Integrated isotope and microbiome analysis indicates dominance of denitrification in N2O production after rewetting of drained fen peat M. Masta et al. 10.1007/s10533-022-00971-3
- Tracing nitrogen transformations during spring development of winter wheat induced by 15N labeled cattle slurry applied with different techniques C. Buchen-Tschiskale et al. 10.1016/j.scitotenv.2023.162061
- Combining N2:Ar and Bayesian Methods to Quantify Underestimation and Uncertainty of Sediment Denitrification Determined by the Acetylene Inhibition Method D. She et al. 10.1007/s11270-019-4100-6
- LDPE and biodegradable PLA-PBAT plastics differentially affect plant-soil nitrogen partitioning and dynamics in a Hordeum vulgare mesocosm M. Reay et al. 10.1016/j.jhazmat.2023.130825
- Denitrification Rate and Its Potential to Predict Biogenic N2O Field Emissions in a Mediterranean Maize-Cropped Soil in Southern Italy A. Forte & A. Fierro 10.3390/land8060097
- Application of isotopic labels optimised for large scale plot experiments A. Tenspolde et al. 10.1007/s00374-023-01730-8
- Modeling Denitrification: Can We Report What We Don't Know? B. Grosz et al. 10.1029/2023AV000990
- The 15N-Gas flux method for quantifying denitrification in soil: Current progress and future directions G. Micucci et al. 10.1016/j.soilbio.2023.109108
- Seasonal variations in N2 and N2O emissions from a wheat–maize cropping system T. Chen et al. 10.1007/s00374-019-01373-8
- Relative Magnitude and Controls of in Situ N2 and N2O Fluxes due to Denitrification in Natural and Seminatural Terrestrial Ecosystems Using 15N Tracers F. Sgouridis & S. Ullah 10.1021/acs.est.5b03513
34 citations as recorded by crossref.
- Determining N2O and N2 fluxes in relation to winter wheat and sugar beet growth and development using the improved 15N gas flux method on the field scale J. Eckei et al. 10.1007/s00374-024-01806-z
- In situ 15N labeling reveals high soil N2 emission during anaerobic soil disinfestation period in a greenhouse vegetable production system X. Li et al. 10.1007/s11104-024-07014-w
- Looking back to look ahead: a vision for soil denitrification research M. Almaraz et al. 10.1002/ecy.2917
- Effects of nitrate and water content on acetylene inhibition technique bias when analysing soil denitrification rates under an aerobic atmosphere H. Yuan et al. 10.1016/j.geoderma.2018.07.039
- Measuring denitrification and the N2O:(N2O + N2) emission ratio from terrestrial soils J. Friedl et al. 10.1016/j.cosust.2020.08.006
- A global synthesis of soil denitrification: Driving factors and mitigation strategies B. Pan et al. 10.1016/j.agee.2021.107850
- Global analysis of agricultural soil denitrification in response to fertilizer nitrogen J. Wang et al. 10.1016/j.scitotenv.2017.10.229
- Microbial processes responsible for soil N2O production in a tropical rainforest, illustrated using an in situ 15N labeling approach W. Zhou et al. 10.1016/j.catena.2021.105214
- Improvement of the 15N gas flux method for in situ measurement of soil denitrification and its product stoichiometry R. Well et al. 10.1002/rcm.8363
- Characteristics of N2 and N2O Fluxes from a Cultivated Black Soil: A Case Study through In Situ Measurement Using the 15N Gas Flux Method Z. Pan et al. 10.3390/agriculture12101664
- Biological nitrogen fixation in peatlands: Comparison between acetylene reduction assay and 15N2 assimilation methods E. Saiz et al. 10.1016/j.soilbio.2019.01.011
- Rain and wind affect chamber measurements M. Maier et al. 10.1016/j.agrformet.2019.107754
- Towards enhanced sensitivity of the 15N gas flux method for quantifying denitrification in soil G. Micucci et al. 10.1016/j.soilbio.2024.109421
- Evaluation of denitrification and decomposition from three biogeochemical models using laboratory measurements of N<sub>2</sub>, N<sub>2</sub>O and CO<sub>2</sub> B. Grosz et al. 10.5194/bg-18-5681-2021
- Mobile continuous-flow isotope-ratio mass spectrometer system for automated measurements of N2 and N2O fluxes in fertilized cropping systems D. Warner et al. 10.1038/s41598-019-47451-7
- Soil Greenhouse Gas Fluxes, Environmental Controls, and the Partitioning of N2O Sources in UK Natural and Seminatural Land Use Types F. Sgouridis & S. Ullah 10.1002/2017JG003783
- Chronic atmospheric reactive N deposition has breached the N sink capacity of a northern ombrotrophic peatbog increasing the gaseous and fluvial N losses F. Sgouridis et al. 10.1016/j.scitotenv.2021.147552
- Biodegradation of biodiesel and toluene under nitrate-reducing conditions and the impact on bacterial community structure H. Ribeiro et al. 10.1007/s11368-018-2079-z
- Isotopomeric Peak Assignment for N2O in Cross-Labeling Experiments by Fiber-Enhanced Raman Multigas Spectroscopy A. Blohm et al. 10.1021/acs.analchem.3c04236
- Underestimation of denitrification rates from field application of the <sup>15</sup>N gas flux method and its correction by gas diffusion modelling R. Well et al. 10.5194/bg-16-2233-2019
- Application methods of tracers for N2O source determination lead to inhomogeneous distribution in field plots J. Berendt et al. 10.1002/ansa.202000100
- Spatial Variations of Soil N2 and N2O Emissions from a Temperate Forest: Quantified by the In Situ 15N Labeling Method D. Xi et al. 10.3390/f13091347
- Rapid Conversion of Added Nitrate to Nitrous Oxide and Dinitrogen in Northern Forest Soil M. Kulkarni et al. 10.1080/01490451.2016.1238981
- Effects of long-term increased N deposition on tropical montane forest soil N2 and N2O emissions W. Tang et al. 10.1016/j.soilbio.2018.08.027
- Quantifying in situ N2 fluxes from an intensively managed calcareous soil using the 15N gas-flux method Y. LIU et al. 10.1016/j.jia.2022.07.016
- Stimulation of soil gross nitrogen transformations and nitrous oxide emission under Free air CO2 enrichment in a mature temperate oak forest at BIFoR-FACE F. Sgouridis et al. 10.1016/j.soilbio.2023.109072
- Integrated isotope and microbiome analysis indicates dominance of denitrification in N2O production after rewetting of drained fen peat M. Masta et al. 10.1007/s10533-022-00971-3
- Tracing nitrogen transformations during spring development of winter wheat induced by 15N labeled cattle slurry applied with different techniques C. Buchen-Tschiskale et al. 10.1016/j.scitotenv.2023.162061
- Combining N2:Ar and Bayesian Methods to Quantify Underestimation and Uncertainty of Sediment Denitrification Determined by the Acetylene Inhibition Method D. She et al. 10.1007/s11270-019-4100-6
- LDPE and biodegradable PLA-PBAT plastics differentially affect plant-soil nitrogen partitioning and dynamics in a Hordeum vulgare mesocosm M. Reay et al. 10.1016/j.jhazmat.2023.130825
- Denitrification Rate and Its Potential to Predict Biogenic N2O Field Emissions in a Mediterranean Maize-Cropped Soil in Southern Italy A. Forte & A. Fierro 10.3390/land8060097
- Application of isotopic labels optimised for large scale plot experiments A. Tenspolde et al. 10.1007/s00374-023-01730-8
- Modeling Denitrification: Can We Report What We Don't Know? B. Grosz et al. 10.1029/2023AV000990
- The 15N-Gas flux method for quantifying denitrification in soil: Current progress and future directions G. Micucci et al. 10.1016/j.soilbio.2023.109108
2 citations as recorded by crossref.
- Seasonal variations in N2 and N2O emissions from a wheat–maize cropping system T. Chen et al. 10.1007/s00374-019-01373-8
- Relative Magnitude and Controls of in Situ N2 and N2O Fluxes due to Denitrification in Natural and Seminatural Terrestrial Ecosystems Using 15N Tracers F. Sgouridis & S. Ullah 10.1021/acs.est.5b03513
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Latest update: 22 Nov 2024
Short summary
Soil denitrification is considered the most un-constrained process in the global N cycle due to uncertain in situ N2 flux measurements, particularly in natural and semi-natural terrestrial ecosystems. The 15N gas-flux method was adapted by lowering the 15N tracer application rate to 0.04–0.5 kg 15N ha−1. The minimum detectable flux rates were 4 μg N m−2 h−1 and 0.2 ng N m−2 h−1 for the N2 and N2O fluxes respectively. The acetylene inhibition technique underestimated denitrification rates by a factor of 4.
Soil denitrification is considered the most un-constrained process in the global N cycle due to...
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