Articles | Volume 13, issue 6
https://doi.org/10.5194/bg-13-1821-2016
https://doi.org/10.5194/bg-13-1821-2016
Research article
 | 
29 Mar 2016
Research article |  | 29 Mar 2016

Application of the 15N gas-flux method for measuring in situ N2 and N2O fluxes due to denitrification in natural and semi-natural terrestrial ecosystems and comparison with the acetylene inhibition technique

Fotis Sgouridis, Andrew Stott, and Sami Ullah

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Soil denitrification is considered the most un-constrained process in the global N cycle due to uncertain in situ N2 flux measurements, particularly in natural and semi-natural terrestrial ecosystems. The 15N gas-flux method was adapted by lowering the 15N tracer application rate to 0.04–0.5 kg 15N ha−1. The minimum detectable flux rates were 4 μg N m−2 h−1 and 0.2 ng N m−2 h−1 for the N2 and N2O fluxes respectively. The acetylene inhibition technique underestimated denitrification rates by a factor of 4.
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