Articles | Volume 14, issue 9
Biogeosciences, 14, 2359–2370, 2017
Biogeosciences, 14, 2359–2370, 2017

Research article 10 May 2017

Research article | 10 May 2017

Nitrogen input 15N signatures are reflected in plant 15N natural abundances in subtropical forests in China

Geshere Abdisa Gurmesa1,2,3, Xiankai Lu1, Per Gundersen2, Yunting Fang4, Qinggong Mao1, Chen Hao5, and Jiangming Mo1 Geshere Abdisa Gurmesa et al.
  • 1Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
  • 2Department of Geosciences and Natural Resource Management, University of Copenhagen, Copenhagen, Denmark
  • 3Sino-Danish Center for Education and Research, Aarhus, Denmark
  • 4CAS Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China
  • 5Huanjiang Observation and Research Station for Karst Ecosystem, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Abstract. Natural abundance of 15N (δ15N) in plants and soils can provide time-integrated information related to nitrogen (N) cycling within ecosystems, but it has not been well tested in warm and humid subtropical forests. In this study, we used ecosystem δ15N to assess effects of increased N deposition on N cycling in an old-growth broad-leaved forest and a secondary pine forest in a high-N-deposition area in southern China. We measured δ15N of inorganic N in input and output fluxes under ambient N deposition, and we measured N concentration (%N) and δ15N of major ecosystem compartments under ambient deposition and after decadal N addition at 50 kg N ha−1yr−1, which has a δ15N of −0.7 ‰. Our results showed that the total inorganic N in deposition was 15N-depleted (−10 ‰) mainly due to high input of strongly 15N-depleted NH4+-N. Plant leaves in both forests were also 15N-depleted (−4 to −6 ‰). The broad-leaved forest had higher plant and soil %N and was more 15N-enriched in most ecosystem compartments relative to the pine forest. Nitrogen addition did not significantly affect %N in the broad-leaved forest, indicating that the ecosystem pools are already N-rich. However, %N was marginally increased in pine leaves and significantly increased in understory vegetation in the pine forest. Soil δ15N was not changed significantly by the N addition in either forest. However, the N addition significantly increased the δ15N of plants toward the 15N signature of the added N, indicating incorporation of added N into plants. Thus, plant δ15N was more sensitive to ecosystem N input manipulation than %N in these subtropical forests. We interpret the depleted δ15N of plants as an imprint from the high and 15N-depleted N deposition that may dominate the effects of fractionation that are observed in most warm and humid forests. Fractionation during the steps of N cycling could explain the difference between negative δ15N in plants and positive δ15N in soils, and the increase in soil δ15N with depths. Nevertheless, interpretation of ecosystem δ15N from high-N-deposition regions needs to include data on the deposition 15N signal.

Short summary
We measured the abundance of a nitrogen (N) isotope (15N) in two subtropical forests in China to study the cycling of input N (deposition and addition). Plant leaves in both forests were 15N-depleted relative to the atmospheric standard, likely as an imprint from 15N-depleted deposition. Plant 15N changed into 15N of the added N, indicating incorporation of N into plants. Thus, interpretations of ecosystem 15N from high-N-deposition regions need to include data on the 15N deposition signature.
Final-revised paper