Articles | Volume 14, issue 17
Biogeosciences, 14, 3979–3994, 2017
Biogeosciences, 14, 3979–3994, 2017

Research article 14 Sep 2017

Research article | 14 Sep 2017

Interplay of community dynamics, temperature, and productivity on the hydrogen isotope signatures of lipid biomarkers

S. Nemiah Ladd1,2, Nathalie Dubois2,3, and Carsten J. Schubert1,4 S. Nemiah Ladd et al.
  • 1Department of Surface Waters – Research and Management, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 6047 Kastanienbaum, Switzerland
  • 2Department of Earth Sciences, ETH Zürich, 8092 Zürich, Switzerland
  • 3Department of Surface Waters – Research and Management, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland
  • 4Institute of Biogeochemistry and Pollutant Dynamics, ETH Zürich, 8092 Zürich, Switzerland

Abstract. The hydrogen isotopic composition (δ2H) of lipid biomarkers has diverse applications in the fields of paleoclimatology, biogeochemistry, and microbial community dynamics. Large changes in hydrogen isotope fractionation have been observed among microbes with differing core metabolisms, while environmental factors including temperature and nutrient availability can affect isotope fractionation by photoautotrophs. Much effort has gone into studying these effects under laboratory conditions with single species cultures. Moving beyond controlled environments and quantifying the natural extent of these changes in freshwater lacustrine settings and identifying their causes is essential for robust application of δ2H values of common short-chain fatty acids as a proxy of net community metabolism and of phytoplankton-specific biomarkers as a paleohydrologic proxy.

This work targets the effect of community dynamics, temperature, and productivity on 2H∕1H fractionation in lipid biomarkers through a comparative time series in two central Swiss lakes: eutrophic Lake Greifen and oligotrophic Lake Lucerne. Particulate organic matter was collected from surface waters at six time points throughout the spring and summer of 2015, and δ2H values of short-chain fatty acids, as well as chlorophyll-derived phytol and the diatom biomarker brassicasterol, were measured. We paired these measurements with in situ incubations conducted with NaH13CO3, which were used to calculate the production rates of individual lipids in lake surface water. As algal productivity increased from April to June, net discrimination against 2H in Lake Greifen increased by as much as 148 ‰ for individual fatty acids. During the same time period in Lake Lucerne, net discrimination against 2H increased by as much as 58 ‰ for individual fatty acids. A large portion of this signal is likely due to a greater proportion of heterotrophically derived fatty acids in the winter and early spring, which are displaced by more 2H-depleted fatty acids as phytoplankton productivity increases. Smaller increases in 2H discrimination for phytol and brassicasterol suggest that a portion of the signal is due to changes in net photoautotrophic 2H fractionation, which may be caused by increasing temperatures, a shift from maintenance to high growth, or changes in the community assemblage. The fractionation factors for brassicasterol were significantly different between the two lakes, suggesting that its hydrogen isotope composition may be more sensitive to nutrient regime than is the case for fatty acids or phytol.

Short summary
Hydrogen isotopes of lipids provide valuable information about microbial activity, climate, and environmental stress. We show that heavy hydrogen in fatty acids declines from spring to summer in a nutrient-rich and a nutrient-poor lake and that the effect is nearly 3 times as big in the former. This effect is likely a combination of increased biomass from algae, warmer temperatures, and higher algal growth rates.
Final-revised paper