Isotopic fractionation of carbon during uptake by phytoplankton across the South Atlantic subtropical convergence
The stable isotopic composition of particulate organic carbon (δ13CPOC) in the surface waters of the global ocean can vary with the aqueous CO2 concentration ([CO2(aq)]) and affects the trophic transfer of carbon isotopes in the marine food web. Other factors such as cell size, growth rate and carbon concentrating mechanisms decouple this observed correlation. Here, the variability in δ13CPOC is investigated in surface waters across the south subtropical convergence (SSTC) in the Atlantic Ocean, to determine carbon isotope fractionation (εp) by phytoplankton and the contrasting mechanisms of carbon uptake in the subantarctic and subtropical water masses. Our results indicate that cell size is the primary determinant of δ13CPOC across the Atlantic SSTC in summer. Combining cell size estimates with CO2 concentrations, we can accurately estimate εp within the varying surface water masses in this region. We further utilize these results to investigate future changes in εp with increased anthropogenic carbon availability. Our results suggest that smaller cells, which are prevalent in the subtropical ocean, will respond less to increased [CO2(aq)] than the larger cells found south of the SSTC and in the wider Southern Ocean. In the subantarctic water masses, isotopic fractionation during carbon uptake will likely increase, both with increasing CO2 availability to the cell, but also if increased stratification leads to decreases in average community cell size. Coupled with decreasing δ13C of [CO2(aq)] due to anthropogenic CO2 emissions, this change in isotopic fractionation and lowering of δ13CPOC may propagate through the marine food web, with implications for the use of δ13CPOC as a tracer of dietary sources in the marine environment.
The marine environment is undergoing rapid changes as atmospheric carbon dioxide increases, with the greatest change occurring in the upper ocean (Gruber et al., 1999; Sabine and Tanhua, 2010). Anthropogenic carbon inputs and the increase of greenhouse gases in the atmosphere are causing ocean warming (Cheng et al., 2019), changes to upper ocean stratification (Bopp et al., 2001; Capotondi et al., 2012), and altered distributions of nutrients and carbon (Khatiwala et al., 2013; Quay et al., 2003; Gruber et al., 2019). Marine phytoplankton are diverse, and are already responding to ocean warming, including changes to productivity (Behrenfeld et al., 2006; Arrigo and van Dijken, 2015), the length of growing season (Henson et al., 2018) and phytoplankton cell size (Finkel et al., 2010). Alterations to phytoplankton diversity and/or productivity will likely have knock-on effects on marine food web dynamics. Investigating such changes in remote marine environments requires tracers that can pinpoint shifts in dietary sources. The δ13C of organic carbon in marine plants and animals can provide information on carbon sources to the base of the food web (Peterson and Fry, 1987). Improved understanding of 13C systematics will lead to reliable use of this proxy in future predictions.
During photosynthesis, marine phytoplankton take up aqueous CO2 ([CO2(aq)]) and convert it into organic carbon. In this process the lighter isotope (12C) is preferentially consumed, leaving the residual aqueous pool increasingly enriched in the heavier isotope. The stable carbon isotopic composition of marine phytoplankton is determined by the uptake fractionation (εp), which is influenced by ambient environmental conditions and phytoplankton cell physiology. Therefore, the δ13C of marine plankton can indicate the controlling mechanisms behind carbon uptake which led to its use as a potential proxy for reconstructing the surface water [CO2(aq)] of past climates (Freeman and Hayes, 1992; Jasper et al., 1994).
The δ13C of particulate organic carbon (δ13CPOC) varies over relatively large oceanic areas and has been found to inversely correlate with [CO2(aq)] (the principal carbon source) in surface waters (Rau et al., 1991; Sackett et al., 1965). High [CO2(aq)] can lead to greater discrimination against 13C during phytoplankton uptake. The low-temperature waters of the Southern Ocean and their high [CO2(aq)] lead to negative δ13C excursions in marine plankton in this region (Sackett et al., 1964). Although this relationship holds true to first order over global datasets (Rau et al., 1989), in many marine environments the local variability in δ13CPOC can be attributed to other mechanisms.
Phytoplankton growth rate, cell size and cell geometry are also important controls on δ13CPOC in surface waters (Bidigare et al., 1997; Francois et al., 1993; Popp et al., 1998; Laws et al., 1995; Villinski et al., 2000). These ecophysiological factors decouple the observed relationship between δ13CPOC and [CO2(aq)], limiting the reliability of δ13CPOC as a palaeoproxy. This is particularly true in areas where [CO2(aq)] is lower or less variable, as other factors have been found to be more important for determining the degree of isotopic fractionation (Henley et al., 2012; Lourey et al., 2004; Popp et al., 1998). In field studies, smaller-sized phytoplankton have been measured with lower δ13CPOC compared with larger cells such as diatoms, particularly in fast growing blooms (Hansman and Sessions, 2016; Rau et al., 1990). These findings indicate that the factors determining δ13CPOC may vary as one transitions contrasting marine environments.
The carbon fixation pathway can vary amongst phytoplankton species through the assimilation of bicarbonate via active transport as opposed to diffusive CO2 uptake. In general, more negative excursions in δ13CPOC are associated with diffusive entry of CO2, whereas carbon concentrating mechanisms (CCMs) or diffusive limitation of carbon supply lead to more positive δ13CPOC (Raven et al., 2008). When [CO2(aq)] falls below a critical level, the active transport of CO2 into the cell can enrich δ13CPOC, but this has been found to be proportional to carbon demand or growth rate (Popp et al., 1998). CCMs occur in most cyanobacteria, increasing CO2 at the site of rubisco activity (Raven et al., 2008).
All of the processes that alter the uptake fractionation of [CO2(aq)] will ultimately determine the isotopic variability in carbon at the base of the marine food web. Thus, the factors that are sensitive to ongoing climate change need to be better understood in order to accurately use δ13CPOC as a tracer of dietary sources and in food web studies.
In this study we investigate the mechanisms for isotopic fractionation in δ13CPOC resulting from carbon uptake and biological production in the upper ocean. We report data from a full transect across the south subtropical convergence (SSTC) in the Atlantic Basin, which captures a region of productive open ocean. The cruise sampled both subantarctic and subtropical regimes with contrasting limiting nutrient environments and community structure (Browning et al., 2014). The [CO2(aq)] and δ13CDIC (stable isotopes of carbon in dissolved inorganic carbon) parameters, along with chlorophyll a and other diagnostic phytoplankton pigments are used collectively to disentangle the processes that fractionate δ13CPOC as a response to the algal uptake of [CO2(aq)] across this region. We find the community cell size, as estimated using the phytoplankton pigment composition, to be the primary determinant of δ13CPOC across the SSTC, with smaller cell sizes increasing the carbon availability for fixation. The results from the field study are used to understand/infer how δ13CPOC in this region may change into the future with ongoing climate change.
2.1 Carbon concentrations and isotopic measurements
Samples were collected onboard the RRS James Cook between December 2011 and February 2012 (JC068), as part of the GEOTRACES A10 transect of the South Atlantic. An east to west transect was conducted with upper ocean sampling at each station. Standard CTD measurements and water sampling were performed using a stainless steel rosette equipped with a full sensor array and 24×20 L OTE bottles. Salinity, temperature and depth were measured using a CTD system (Seabird 911+), and salinity was calibrated on-board with discrete samples using an Autosal 8400B salinometer (Guildline).
Measurements of total CO2 (TCO2) and total alkalinity (TA) were carried out at sea within 24 h of collection. Samples were warmed in a water bath at 25 ∘C for 1 h before analysis. A set volume of the sample was acidified by addition of excess 10 % phosphoric acid, which converts all inorganic C species to CO2. This is carried into the coulometric cell by an inert carrier gas (CO2-free N2 that is first passed through a magnesium perchlorate and Ascarite II scrubber), and a coulometric titration determines the amount of CO2, which is equal to TCO2. Small increments of 0.1 M hydrochloric acid are added to a separate subsample and the amount added to reach the carbonic acid equivalence point is equal to the TA (Humphreys, 2015). Regular measurements of both TCO2 and TA were made from batch 114 certified reference material (CRM) from Andrew G. Dickson (Scripps Institution of Oceanography; Dickson et al., 2003) and used to calibrate the results. To obtain the final results in micromoles per kilogram ( µmol kg−1), a correction for density (ρ) due to salinity variation was then applied using salinity measured from Niskin bottle samples (Zeebe et al., 2001). Duplicate samples were taken from the same Niskin bottle and analysed consecutively.
[CO2(aq)] was calculated from measured TA and DIC using CO2SYS v1.1 (Lewis and Wallace, 1998; van Heuven et al., 2011). Equilibrium constants were evaluated following Mehrbach et al. (1973) for carbonic acid and Dickson (1990) for bisulfate, and the boron : chlorinity ratio of Lee et al. (2010) was used.
Samples for the measurement of the stable isotopes of carbon in dissolved inorganic carbon (δ13CDIC) were collected from the stainless steel rosette. Samples were taken into 250 mL glass bottles with ground glass stoppers. Water was drained directly into the sample bottle using silicone tubing to the bottom of the bottle to eliminate bubble formation. The bottle and cap were rinsed once with water from the rosette bottle before overflowing the sample bottle by at least one bottle volume before withdrawing the silicone tube, carefully avoiding bubble formation. Next, the stopper was placed in the bottle and then removed so that 2.5 mL of sample could be removed to allow for thermal expansion, and 50 µL of 100 % HgCl2 was added to halt any biological activity. The stoppers and the inside of the neck of the bottles were dried before the stopper, coated with vacuum grease, was replaced and secured with a foam insert and plastic cover. The samples were then shaken to disperse the HgCl2 and stored at 4 ∘C until analysis. Samples were measured using a Thermo MAT253 stable isotope mass spectrometer. was determined from δ13CDIC and absolute temperature (Tk), using (Rau et al., 1996).
Particulate samples were collected onto ashed, pre-weighed GF/F microfibre filters (0.7 µm pore size, 25 mm diameter). Two to four litres of water was collected from the biological rosette in the surface 400 m depending on the chlorophyll levels detected by the CTD fluorometer. The samples were pressure-filtered simultaneously using an eight-way manifold system. Once the total volume for each depth was filtered, the filters were extracted from the filter holder, placed in labelled aluminium foil and dried at 50∘ for ∼12 h. Once dried, filters were folded and stored in plastic sample bags at −20 ∘C. To remove carbonates prior to analysis, filters were wetted with Milli-Q water, fumed with 70 % HCl for 48 h in a desiccator, dried at 50 ∘C and then folded into tin capsules. The filters were analysed using a Carlo Erba NA 2500 elemental analyser in-line with a VG PRISM III isotope ratio mass spectrometer for elemental POC/PN as well as δ13CPOC and δ15NPN. All δ13CPOC data presented in this study are in the delta per mil notation vs. V-PDB (‰VPDB).
2.2 Cell size calculations
Phytoplankton pigments were analysed by high-performance liquid chromatography (HPLC) analysis. Between 500 and 2000 mL of seawater was filtered through 25 mm GF/F filters. The filters were placed in 2 mL cryovials and flash frozen in liquid nitrogen. Next, filters were transferred to a −80 ∘C freezer for longer-term storage. Pigment extracts were analysed with a reverse-phase HPLC column using a Thermo Finnigan HPLC instrument at the National Oceanography Centre Southampton (Gibb et al., 2000). Phytoplankton pigments were extracted in 3 to 5 mL of 90 % acetone by ultrasonication and centrifugation. Extracts were loaded into a chilled autosampler prior to injection into the HPLC system. Pigments were detected by absorbance at 440 nm and identified by diagnostic retention times. The resulting pigment assemblage was used to estimate the fractional contribution of the three size classes (micro-, nano- and picophytoplankton) to the total chlorophyll a pigment concentration (Bricaud et al., 2004; Uitz et al., 2008).
3.1 Oceanographic setting
The SSTC is characterized by the convergence of contrasting biogeochemical regimes. In the colder Subantarctic Surface Waters (SASW), located south of the SSTC, concentrations of macronutrients are elevated and primary production is primarily limited by iron availability (Browning et al., 2014). The subtropical waters to the north of the SSTC are associated with the South Atlantic subtropical gyre and are principally macronutrient limited, or possibly macronutrient–iron co-limited (Browning et al., 2014, 2017). The three subtropical water masses, the Agulhas Current (AC), the South Atlantic Central Water (SACW) and the Brazil Current (BC), can be readily identified by warmer temperatures and higher salinities; the influence of the Malvinas Current (MC) separates the core of the SACW and BC (Fig. 1).
Higher [CO2(aq)] is associated with the lower temperatures of the SASW. Across the zonal transect, higher is associated with lower [CO2(aq)] and warmer temperatures of the subtropical water masses (Fig. 1). is highest on the western boundary in the BC and in the Rio de la Plata outflow. δ13CPOC across 40∘ S ranges from −25 ‰ to −20 ‰ indicating a predominantly marine source (e.g. Rau et al., 1989).
Satellite images of surface chlorophyll concentrations across this region indicate elevated standing stocks of phytoplankton in comparison with the South Atlantic gyre and subantarctic waters further south (Browning et al., 2014). Chlorophyll concentrations peak between austral spring and summer, and the south subtropical convergence (SSTC) moves south as a result of the expansion of the Agulhas and Brazil currents. Depth profiles showed that the subantarctic waters have elevated and uniform chlorophyll concentrations (0.2–0.9 mg m−3). Conversely, in the subtropical waters, a deep chlorophyll maximum is formed, with low surface concentrations of chlorophyll (<0.2 mg m−3) and macronutrients (Tuerena et al., 2015).
3.2 δ13CPOC variability
If δ13CPOC is principally determined by changing ambient [CO2(aq)] and is not influenced by cell physiology, such as growth rates and cell size, the δ13CPOC can often be predicted by sea surface temperature variability (Rau et al., 1989). In this study, δ13CPOC is compared to a model from Rau et al. (1996), which predicts the carbon isotope fractionation (εp) and δ13CPOC where photosynthesis is strictly based on the passive diffusion of CO2 into marine phytoplankton cells.
Modelled δ13CPOC was calculated using the diffusion model of Rau et al. (1996), where
Here εf represents intracellular enzymatic isotope fractionation (‰), εd represents diffusive isotope fractionation of CO2(aq) in seawater (‰), Qs represents CO2 uptake rate per unit cell surface area (mol C m−2 s−1), [CO2(aq)] refers to ambient CO2(aq) concentration (mol m−3), r is the cell radius (m), DT is the temperature dependent diffusion rate of CO2 (m2 s−1), rk is the reacto-diffusive length (m) and P represents the cell wall permeability to CO2 (m s−1). Qs was determined from
where γc is the carbon content per cell (mol C), and μi is the cell growth rate (s−1).
Using this model we tested how δ13CPOC and εp vary as a function of [CO2(aq)], temperature, growth rate and cell size. For each of these parameters we used the base values of Rau et al. (1996) unless specified otherwise.
εp for measured and modelled δ13C was calculated as follows:
To understand under the conditions under which the ambient [CO2(aq)] plays a dominant role in the determination of δ13CPOC in surface waters across the frontal region, the relationships between δ13CPOC, and CO2(aq) were compared to modelled estimates for passive diffusion (Fig. 2). SASW samples fall close to the modelled estimates, and subtropical samples decouple from the modelled trend to a much higher degree. In Fig. 2a, [CO2(aq)] and in the SASW have a significant negative correlation (, n=12, p=0.003), and correspond to the model trends, with lower concentrations resulting in higher δ13CDIC and . There are no significant correlations between δ13CPOC and [CO2(aq)] or in the subtropical or subantarctic water masses (Fig. 2, p>0.05). These data suggest that although [CO2(aq)] may play a role in determining the δ13CPOC in the SASW, other factors cause deviation away from a significant correlation, with the relationship increasingly decoupled in subtropical waters.
To investigate the spatial variability across the SSTC, [CO2(aq)], and were plotted against longitude and compared to model estimates (Rau et al., 1996; Supplement), where we used the model constants for cell size (10 µm) and reconstructing [CO2(aq)] from temperature variability across the transect (Fig. 3a, b). Temperature can predict the spatial variability in [CO2(aq)], and , but ambient [CO2(aq)] is lower and is higher than model estimates, resulting from biological production and the isotopic disequilibrium between the ocean and atmosphere (Gruber et al., 1999). δ13CPOC is predicted using ambient temperature as well as measurements of [CO2(aq)] and (Fig. 3c). There is no correlation between measured and modelled δ13CPOC across the transect, suggesting that there are other controlling factors which determine the δ13CPOC variability.
To test whether cell size (and thus the cellular surface area to volume ratio) plays an important role in determining δ13CPOC, we estimated the change in phytoplankton size classes across the transect. Using phytoplankton pigment data we calculated the relative proportion of pico-, nano- and microphytoplankton size fractions to total chlorophyll a biomass (Bricaud et al., 2004; Uitz et al., 2008). Picophytoplankton were more abundant in the subtropical environments than in the SASW, contributing between 30 % and 40 % of the pigment biomass at the core of these water masses (Fig. 4). In contrast, nano- and microphytoplankton were more dominant in the SASW and close to the Rio de la Plata outflow.
An estimate of the approximate average community cell size was calculated by defining a specific cell size for each of the three defined size classes (picophytoplankton were defined as 1 µm, nanophytoplankton were defined as 5 µm and microphytoplankton were defined as 50 µm; Bricaud et al., 2004). The central size values for each class were divided by two to approximate the average community cell radius (picophytoplankton were 0.5 µm, nanophytoplankton were 2.5 µm and microphytoplankton were 25 µm). This method only provides a rough indicator of the community cell radius, as size class is represented by one unique size for each algal group; however, this enables one single parameter to be used to characterize the size structure of the algal population, which is important for the purposes of this study.
Estimated average cell radii were generally smaller at the core of the subtropical water masses compared with the SASW (Fig. 5; depth range <40 m, subtropical, >20 ∘C, 6.5 µm ±0.8, n=17; subantarctic, <18 ∘C, 10.4 µm ±2.3, n=31). Increasing the average cell size (and thus decreasing SA : V) has the potential to reduce carbon isotope fractionation during uptake by passive diffusion and, thus, increase δ13CPOC by reducing the ability of the cell to discriminate between the two isotopes. This has been found in modelled, experimental and environmental studies (Popp et al., 1998; Pancost et al., 1997; Rau et al., 1990, 1996).
When δ13CPOC is modelled using temperature, SASW measurements fall between model estimates for a cell radius of 10–15 µm (Fig. 6a). Conversely, the subtropical samples have higher proportions of picoplankton (<2 µm), and decrease to lower δ13CPOC than those predicted using temperature alone, demonstrating that cell size is likely a controlling factor in δ13CPOC determination. The average community cell radius in open ocean samples was compared to δ13CPOC (Fig. 6b), and a significant positive correlation was observed (r=0.74, n=30, p<0.001).
The samples with a larger estimated community cell size on both the east and western margins were not included in this correlation analysis as they show a significant offset from this relationship (Fig. 6b). These samples have a larger estimated cell size compared with measured δ13CPOC and suggest that there is a possible terrestrial influence, either with the supply of allochthonous material, the presence of grazers and/or significant shifts in the species assemblage to a higher abundance of microplankton (Browning et al., 2014). The significant positive correlation between cell size and δ13CPOC for open ocean waters suggests that cell size is the primary factor influencing δ13CPOC in the surface waters across the SSTC. We further test the relationship between δ13CPOC and cell size by predicting changes in δ13CPOC using temperature and cell size measurements (black crosses in Fig. 6b). We find good agreement between modelled and measured data points, demonstrating the importance of cell size in estimating δ13CPOC.
South of the SSTC, the phytoplankton community is dominated by haptophytes (Browning et al., 2014). A lower species diversity south of the front may explain the closer alignment between [CO2(aq)] and δ13CPOC, as other factors are less significant in influencing εp. Recent work has highlighted the interspecies differences in carbon uptake fractionation and their influence on bulk δ13CPOC (Hansman and Sessions, 2016). Our results suggest a changing community cell size deviates δ13CPOC from expected trends with [CO2(aq)]. In this open ocean environment, using estimates of cell size in addition to [CO2(aq)], we can predict variability in δ13CPOC.
4.1 Carbon uptake fractionation across the 40∘ S transect
The biological fractionation of carbon isotopes during uptake by phytoplankton can be estimated using (Freeman and Hayes, 1992). This fractionation comprises both the CO2 fixation during photosynthesis, which utilizes the enzyme rubisco ( ‰ to −31 ‰), and is also determined by the factors which limit the external supply of CO2 to the enzyme. Therefore, the more CO2-limited the cell, the less the isotopic fractionation of CO2 fixation will be expressed. These limiting factors include ambient [CO2(aq)] (Baird et al., 2001), growth rates (Laws et al., 1995; Popp et al., 1998), cell size or geometry (Popp et al., 1998), light availability and day length (Laws et al., 1995; Burkhardt et al., 1999), utilization of HCO in replacement of CO2 (Sharkey et al., 1985) and species variability (Falkowski, 1991).
Empirical estimates of εp range between 10 ‰ and 18 ‰, with the highest fractionation observed in the Southern Ocean where [CO2(aq)] is highest, increasing to over 20 µM in surface waters (Young et al., 2013). Over the Atlantic SSTC we measure an εp range of 12 ‰–17 ‰. In the subtropical water masses north of the SSTC, the average εp is 1 ‰ higher than in the SASW despite lower [CO2(aq)] (Fig. 7). Our data contrast the global observed variability (of high εp in high [CO2(aq)] regions such as the Southern Ocean) but are comparable to results from previous work in frontal regions where higher εp has been observed in lower [CO2(aq)] subtropical water masses (Bentaleb et al., 1998; Francois et al., 1993).
We predict the variability in εp using temperature, [CO2(aq)], and changes in community cell size across the region. If [CO2(aq)] was the controlling mechanism behind εp, increases in [CO2(aq)] would result in increased εp. There is no significant trend between modelled εp (temperature, [CO2(aq)]) and measured εp (Fig. 7a). In contrast, when cell size is included, there is a significant positive correlation (Fig. 7c; r=0.72, p=0, df=18). These results indicate that there is an inverse trend between modelled εp and [CO2(aq)] in this region: εp increases with decreasing [CO2(aq)], which can be best attributed to the variability in the gross size structure of the phytoplankton assemblage across the SSTC (Fig. 7).
If the flow of [CO2(aq)] into and out of a cell is determined by gas diffusion, then the flow is proportional to the cell surface area. A decrease in cell radius leads to an increase in cell surface area to volume ratio (SA : V), increasing the amount of [CO2(aq)] diffusing across the cell membrane relative to the total carbon within the cell, and allowing greater fractionation and higher εp. Thus, εp has been found to be negatively correlated with phytoplankton cell size, with larger cells such as diatoms showing less isotopic fractionation compared with smaller phytoplankton (Popp et al., 1998; Hansman and Sessions, 2016). These cell size trends are observed across our open ocean transect, with the largest cell sizes having lower εp and higher δ13CPOC.
The influence of cell size on the expression of εp is likely to have a greater effect with increasing growth rate (Rau et al., 1996; Popp et al., 1998). A higher growth rate, such as in spring/summer blooms increases the range of εp expressed across cell sizes. For instance in fast growing blooms, larger cell sizes may have higher relative δ13CPOC and lower εp than smaller cell sizes, compared with low growth periods (e.g. Fry and Wainwright, 1991). The SSTC is a dynamic nutrient environment (Ito et al., 2005), with the convergence of N-limited subtropical waters (Eppley et al., 1979), with the iron-limited Antarctic Circumpolar Current (ACC) waters (Boyd et al., 2000; Browning et al., 2014). The convergence of contrasting regimes potentially increases nutrient availability to phytoplankton whilst also contributing to the thermal stability of the upper water column (Longhurst, 1998), thus having the potential to elevate growth rates. Therefore, the expression of cell size on εp is intuitive in this environment. An increase in εp with decreasing cell size has been noted in previous work (Goericke and Fry, 1994) and may be the primary driver for community εp in the SSTC during spring and summer, when growth rates are high.
4.2 Regional and global factors influencing uptake fractionation
The changing [CO2(aq)] is the principal determinant of δ13CPOC across the global ocean (Sackett et al., 1965; Rau et al., 1989; Goericke and Fry, 1994). A modelling study found that the inter-hemispheric differences in δ13CPOC could be explained by the inter-hemispheric asymmetry in [CO2(aq)] (Hofmann et al., 2000). Poleward of ∼50∘ S, [CO2(aq)] ranges between 15 and 25 µM, δ13CPOC between −30 ‰ and −24 ‰, and εp is greatest of anywhere in the global ocean (Fig. 8). The fractionation during carbon fixation (rubisco) is highly expressed on εp and other factors are less influential.
In the low-latitude ocean, previous studies have shown that this trend becomes decoupled: [CO2(aq)] decreases, growth rates are more variable and community structure and seasonal dynamics decouple the observed correlation of fractionation with temperature (e.g. Francois et al., 1993; Bentaleb et al., 1998). Previous studies of εp at the SSTC found decoupling between δ13CPOC and [CO2(aq)], attributed to changing physical processes across the frontal region (Francois et al., 1993; Bentaleb et al., 1998). The variable water mass movements decouple trends, and it has been suggested that δ13CPOC variability in water masses can result from the local phytoplankton assemblage (Fontugne and Duplessy, 1978). Strong seasonal variations in δ13CPOC can also result from changes in biological parameters such as cell radius, cell membrane permeability and growth rate (Francois et al., 1993; Goericke and Fry, 1994; Jasper et al., 1994; Laws et al., 1995; Popp et al., 1998). Phytoplankton assemblage-derived changes in εp have been observed in other changing environments, such as the seasonal sea ice zone (Dehairs et al., 1997; Popp et al., 1999) and in major frontal regions (Dehairs et al., 1997; Popp et al., 1999; this study).
The results from our field study demonstrate that the phytoplankton assemblage has a key role in determining εp and δ13CPOC from their cell size and physiology, likely linked to the high growth rates in this frontal region. We test whether cell size variability presents a control over εp across meridional transects (Fig. 8). We find no relatable trend on a global scale, and latitudinal trends demonstrate an increase in εp with an increase in [CO2(aq)]. However, increased cell size reduces the expression of a high εp (as shown by the higher δ13CPOC and lower εp in Fig. 8c and d), which is particularly evident between 30 and 60∘ S.
Thus, regions where frequent physical changes stimulate variable and diverse phytoplankton assemblages may be more likely to have a decoupled relationship between εp and [CO2(aq)]. We suggest that the high growth rates across this region play an important role in driving this change – we sampled across the SSTC in summer (high light levels), which may further promote the importance of cell size in determining δ13CPOC.
4.3 Changes to uptake fractionation and δ13CPOC in response to climate change
Ambient [CO2(aq)] is increasing in the global ocean. A recent study found that εp has increased significantly since the 1960s in the subtropical Atlantic, whereas no notable change has been detected in polar regions (Young et al., 2013). Our results suggest that a change in the community cell size would impact εp, with a decrease in cell radius leading to increased εp. Thus, a changing community structure with the onset of climate change may further impact the εp and δ13CPOC, and have implications for our understanding of carbon isotope variability at the base of the food web.
Observational studies show rapid warming in the world's oceans in response to climate change, and that most of the ocean heat uptake is stored in the upper 75 m (Cheng et al., 2019). Predicted ocean warming trends are variable in different regions, with a greater rate of increase predicted in the polar regions (IPCC, 2013). Warming at the ocean surface promotes thermal stratification, which, in the Southern Ocean may decrease light limitation, whereas in the subtropics it is likely to promote further nutrient limitation (Sarmiento et al., 2004). Thus, climate change will promote varying responses from phytoplankton communities and their physiology across the global ocean (Rousseaux and Gregg, 2015).
In the oligotrophic gyres, ocean–atmosphere global climate models project increased stratification and decreases in net primary productivity with the onset of climate change (Boyd and Doney, 2002; Capotondi et al., 2012; Le Quere et al., 2003). Nutrients are already the limiting factor for net primary productivity and small cells are readily adapted to these oligotrophic environments, where recycled nutrients such as ammonium are the main nutrients available to phytoplankton (Fawcett et al., 2011). Many studies observe a shift to phytoplankton communities dominated by picoplankton as the water column becomes stratified and increasingly nutrient depleted (Atkinson et al., 2003; Bouman et al., 2003; Latasa and Bidigare, 1998; Lindell and Post, 1995; Irwin and Oliver, 2009). These observations suggest that the average community cell size may decrease further with ongoing climate change.
There may be large-scale shifts in community structure, including the physiological dependencies of phytoplankton on light and nutrients and their ecological diversity (Bouman et al., 2005; Behrenfeld et al., 2005; Siegel et al., 2005). A decrease in cell size may lead to a faster pace of metabolism (Brown et al., 2004). However, recent work suggests that CO2 fixation and respiration rates are unlikely to increase under nutrient limiting conditions (Maranon et al., 2018). Therefore, subtropical regions may simultaneously experience warming, decreases in nutrient supply, increases in CO2 availability, decreases in cell size and changes to community structure.
At higher latitudes, models predict increases in net primary production with improved light availability in the mixed layer and an extended growing season (Bopp et al., 2001; Sarmiento et al., 2004). Warming and reduction in sea ice is likely to initiate an earlier onset of bloom with a predicted 5–10 d shift per decade (Henson et al., 2018). Therefore, in the subantarctic ocean we may expect decreased light limitation, higher growth rates and decreases in community cell size. The results from this study demonstrate the different physiology of phytoplankton across the SSTC and the expression of carbon uptake on δ13C fixed into the phytoplankton cell. We find that δ13CPOC can be predicted using variability in cell size and [CO2(aq)]. Furthermore, the subtropical and subantarctic uptake fractionation may respond differently with changing ambient [CO2(aq)] and temperature with predicted future climate warming scenarios.
We use the results from this study to predict how the isotopic fractionation during carbon uptake may alter with increased [CO2(aq)] as a response to climate change. To do so, we alter the model inputs to increase atmospheric CO2 from 400 to 500 ppm, thereby increasing [CO2(aq)] in the surface ocean, which we calculate using the solubility coefficients of CO2 in seawater (Fig. 9, Weiss, 1974). We test variability from an average cell radius of 5, 10 and 20 µm and investigate the changes over the temperature range of the ocean. Although a smaller cell size (radius 5 µm) fractionates δ13C to a greater degree than a larger cell, there is only a 1 ‰ increase in εp with a 100 ppm increase in atmospheric CO2 concentrations (Fig. 9a). Therefore, changing the CO2 concentration alone may not have a large effect on δ13CPOC in subtropical environments. Instead, a trend to smaller average cell size would have a much greater impact on the δ13CPOC which is observed and predicted in the oligotrophic gyres.
In the subantarctic waters, although predicted εp is lower in the larger cell sizes expected south of the SSTC, an increase in ambient CO2 concentrations would have a much larger effect on εp. There is an observed 3 ‰ increase in εp in larger phytoplankton (radius 20 µm) with a 100 ppm increase in atmospheric CO2 concentration. The changing conditions in the subantarctic ocean with the onset of climate change are also likely to promote the success of smaller-sized phytoplankton, as the light conditions improve and the upper ocean becomes increasingly stratified in summer months (Bopp et al., 2001). A decrease in community cell size could also increase εp and produce lower δ13CPOC. These results indicate that the subantarctic ocean, which has a relatively larger cell size in comparison with the subtropical ocean and is predicted to become increasingly stratified, may experience a greater change in the δ13CPOC produced during photosynthesis over the upcoming decades.
The sensitivity of δ13CPOC to increases in anthropogenic carbon is determined by the change in [CO2(aq)] and also its isotopic signature. Enhanced diffusion of anthropogenic CO2 between the atmosphere and the ocean's surface increases concentrations of [CO2(aq)] in the ocean (Friedli et al., 1986; Francey et al., 1999). Anthropogenic CO2 is enriched in the lighter 12C isotope, so its invasion into the ocean decreases δ13CDIC in a phenomenon known as the Suess effect (Keeling, 1979), which has been observed across the ocean over the last decade (Quay et al., 2003). The uptake of anthropogenic CO2 by the world's oceans has led to a decrease in δ13CDIC of 0.025 % yr−1 (Gruber et al., 1999). The increase in seawater pCO2 from 400 to 500 µatm shown in Fig. 9 corresponds to DIC increasing by between 30 and 50 µmol kg−1 (with a greater DIC increase at higher temperatures). Assuming a ratio of anthropogenic CO2 invasion to δ13CDIC change (i.e. ΔRC) of about −0.016 ‰ (µmol kg in this region (Heimann and Maier-Reimer, 1996; McNeil et al., 2001), the associated Suess effect could decrease δ13CDIC – and therefore δ13CPOC – by an extra 0.5 ‰ to 0.8 ‰, consistently across all cell sizes (Fig. 9b). This decrease would be in addition to, and independent from, any change due to fractionation, and consistent in magnitude for every cell size.
Seawater warming, which is expected to accompany future increases in [CO2(aq)], independently modulates the marine carbonate system (Humphreys, 2017) and the fractionation model of Rau et al. (1996). In this case, simultaneous warming would oppose the increase in εp (and therefore decrease in δ13CPOC) driven by increasing [CO2(aq)], as shown by the negative line gradients in Fig. 9a (and positive gradients in Fig. 9b). However, this is expected to have a relatively small impact overall, as the following back-of-the-envelope calculation illustrates. Given an equilibrium climate sensitivity (i.e. the equilibrium warming of Earth's near-surface resulting from a doubling of atmospheric pCO2) of 1.5 to 4.5 ∘C (Stocker et al., 2013), an increase in pCO2 from 400 to 500 ppm would drive 0.5 to 1.5 ∘C of global mean warming. For 10 µm cells, the pCO2 change alone would increase εp by ∼1.8 ‰, while this warming alone would decrease εp by only 0.1 to 0.4 ‰, according to the model of Rau et al. (1996).
Stable isotope analysis of organic matter has emerged as the primary means of examining marine food web structure and variability (Middelburg, 2014). Carbon isotope signatures in particulate organic carbon vary substantially from the relative influence of terrestrial and marine carbon, carbon uptake pathways and the influence of carbon concentrating mechanisms (Jasper and Gagosian, 1990; Ganeshram et al., 1999). In contrast to nitrogen isotopes, there is negligible fractionation of carbon isotopes through trophic levels, which allows for the accurate estimation of dietary sources of carbon (Minagawa and Wada, 1984). Therefore, the factors that contribute to variability at the base of the food web need to be well understood in order to accurately comprehend marine food web dynamics (Peterson and Fry, 1987). These findings could also have implications for the distribution of δ13CPOC in the deep ocean via organic matter sinking and burial (e.g. Cavagna et al., 2013).
This study highlights the importance of cell size as a primary determinant of the extent of isotopic fractionation in particulate organic carbon during uptake and the subsequent signature imparted at the base of the food web. Our findings support previous work predicting increases in εp and decreases in δ13CPOC in the future (Young et al., 2013). However, we suggest that increasing εp may predominantly result from shifts in community structure towards smaller-sized phytoplankton. Changes in phytoplankton assemblages are being detected globally (Rousseaux and Gregg, 2015), in addition to possible declines in phytoplankton biomass (Boyce et al., 2010). Our detailed study of the subtropical and subantarctic environments predict greater relative decreases in δ13CPOC in polar regions than in the subtropics in response to changing [CO2(aq)]. If increased stratification proceeds in the subantarctic, this may also lead to decreases in average cell size and, thus, even greater decreases in δ13CPOC.
δ13CPOC measurements from the SSTC in the Atlantic Ocean are compared to model predictions to determine the factors which control δ13C variability and carbon uptake fractionation (εp). Our results contrast global trends in marine waters, where δ13CPOC is lower in high CO2 environments as a result of increased carbon uptake fractionation. Instead we find the δ13CPOC and εp are largely determined by community cell size variability, which we estimate using phytoplankton pigment composition. We measured a greater εp in the subtropical water masses where smaller-sized phytoplankton are more dominant and can fractionate δ13C to a greater degree by the increased CO2 availability to the enzyme rubisco as a result of their enhanced increase surface area to volume ratio. Our results suggest a greater variability in δ13C and εp as a result of community cell size than previously predicted and highlight the need to understand the phytoplankton community structure.
We use our results from the field study to understand how increased CO2 availability in the future will affect the carbon isotope fractionation in phytoplankton. Our findings suggest that larger-celled phytoplankton in the subantarctic may respond more to changes in the carbon concentration. However, shifts in algal assemblages towards smaller phytoplankton will also have a large effect on the community εp expressed. These results suggest that decreasing cell size and increased CO2 availability to phytoplankton will increase εp and decrease δ13CPOC. Our study illustrates that phytoplankton cell size changes in response to warming may alter δ13C at the base of the food chain, and need to be taken into account along with the Suess effect when using δ13C as a food source tracer.
All carbon and isotope data have been submitted to the British Oceanographic Data Centre as part of the UK GEOTRACES programme. The data in this study are also available on request from the corresponding author.
The supplement related to this article is available online at: https://doi.org/10.5194/bg-16-3621-2019-supplement.
RET analysed the δ13C of particulate organic carbon, MPH measured dissolved inorganic carbon and alkalinity, AP analysed the δ13C of dissolved inorganic carbon, and TJB and HB measured phytoplankton pigments. RET and RSG designed and wrote the paper, all authors contributed to the final version of the paper.
The authors declare that they have no conflict of interest.
We thank the crew and scientists of the RRS James Cook (JC068) and Gideon Henderson for coordination of the UK GEOTRACES 40∘ S transect. This work was funded by the UK GEOTRACES National Environment Research Council (NERC) consortium grant (grant no. NE/H008497/1NERC) which included a studentship for Robyn E. Tuerena.
This research has been supported by the NERC (grant no. NE/H008497/1NERC).
This paper was edited by Christoph Heinze and reviewed by two anonymous referees.
Arrigo, K. R. and van Dijken, G. L.: Continued increases in Arctic Ocean primary production, Prog. Oceanogr., 136, 60–70, https://doi.org/10.1016/j.pocean.2015.05.002, 2015.
Atkinson, D., Ciotti, B. J., and Montagnes, D. J. S.: Protists decrease in size linearly with temperature: ca. 2.5 % degrees C-1, P. Roy. Soc. B-Biol. Sci., 270, 2605–2611, https://doi.org/10.1098/rspb.2003.2538, 2003.
Baird, M. E., Emsley, S. M., and Mcglade, J. M., Using a phytoplankton growth model to predict the fractionation of stable carbon isotopes, J. Plankt. Res., 23, 8, 841–848, https://doi.org/10.1093/plankt/23.8.841, 2001.
Behrenfeld, M. J., Boss, E., Siegel, D. A., and Shea, D. M.: Carbon-based ocean productivity and phytoplankton physiology from space, Global Biogeochem. Cy., 19, GB100, https://doi.org/10.1029/2004gb002299, 2005.
Behrenfeld, M. J., O'Malley, R. T., Siegel, D. A., McClain, C. R., Sarmiento, J. L., Feldman, G. C., Milligan, A. J., Falkowski, P. G., Letelier, R. M., and Boss, E. S.: Climate-driven trends in contemporary ocean productivity, Nature 444, 752–755, https://doi.org/10.1038/nature05317, 2006.
Bentaleb, I., Fontugne, M., Descolas-Gros, C., Girardin, C., Mariotti, A., Pierre, C., Brunet, C., and Poisson, A.: Carbon isotopic fractionation by plankton in the Southern Indian Ocean: relationship between delta C-13 of particulate organic carbon and dissolved carbon dioxide, J. Marine Syst., 17, 39–58, https://doi.org/10.1016/s0924-7963(98)00028-1, 1998.
Bidigare, R. R., Fluegge, A., Freeman, K. H., Hanson, K. L., Hayes, J. M., Hollander, D., Jasper, J. P., King, L. L., Laws, E. A., Milder, J., Millero, F. J., Pancost, R., Popp, B. N., Steinberg, P. A., and Wakeham, S. G.: Consistent fractionation of C-13 in nature and in the laboratory: Growth-rate effects in some haptophyte algae, Global Biogeochem. Cy., 11, 279–292, https://doi.org/10.1029/96gb03939, 1997.
Bopp, L., Monfray, P., Aumont, O., Dufresne, J. L., Le Treut, H., Madec, G., Terray, L., and Orr, J. C.: Potential impact of climate change on marine export production, Global Biogeochem. Cy., 15, 81–99, https://doi.org/10.1029/1999gb001256, 2001.
Bouman, H. A., Platt, T., Sathyendranath, S., Li, W. K. W, Stuart, V., Fuentes Yaco, C., Maass, H., Horne, E. P. W., Ulloa, O., Lutz, V., and Kyewalyanga, M.: Temperature as an indicator of the optical properties and gross community structure of marine phytoplankton: implications for remote sensing of ocean colour, Mar. Ecol. Progr. Ser., 258, 19–30, 2003.
Bouman, H. A., Platt, T., Sathyendranath, S., and Stuart, V: Dependence of light-saturated photosynthesis on temperature and community structure, Deep-Sea Res. I, 52, 1284–1299, 2005.
Boyce, D. G., Lewis, M. R., and Worm, B.: Global phytoplankton decline over the past century, Nature, 466, 591–596, https://doi.org/10.1038/nature09268, 2010.
Boyd, P. W. and Doney, S. C.: Modelling regional responses by marine pelagic ecosystems to global climate change, Geophys. Res. Lett., 29, 53-1–53-4, https://doi.org/10.1029/2001gl014130, 2002.
Boyd, P. W., Watson, A. J., Law, C. S., Abraham, E. R., Trull, T., Murdoch, R., Bakker, D. C. E., Bowie, A. R., Buesseler, K. O., Chang, H., Charette, M., Croot, P., Downing, K., Frew, R., Gall, M., Hadfield, M., Hall, J., Harvey, M., Jameson, G., LaRoche, J., Liddicoat, M., Ling, R., Maldonado, M. T., McKay, R. M., Nodder, S., Pickmere, S., Pridmore, R., Rintoul, S., Safi, K., Sutton, P., Strzepek, R., Tanneberger, K., Turner, S., Waite, A., and Zeldis, J.: A mesoscale phytoplankton bloom in the polar Southern Ocean stimulated by iron fertilization, Nature, 407, 695–702, 2000.
Bricaud, A., Claustre, H., Ras, J., and Oubelkheir, K.: Natural variability of phytoplanktonic absorption in oceanic waters: Influence of the size structure of algal populations, 109, C11010, https://doi.org/10.1029/2004JC002419, 2004.
Brown, J. H., Gillooly, J. F., Allen, A. P., Savage, V. M., and West, G. B.: Toward a metabolic theory of ecology, Ecology, 85, 1771–1789, https://doi.org/10.1890/03-9000, 2004.
Browning, T. J., Bouman, H. A., Moore, C. M., Schlosser, C., Tarran, G. A., Woodward, E. M. S., and Henderson, G. M.: Nutrient regimes control phytoplankton ecophysiology in the South Atlantic, Biogeosciences, 11, 463–479, https://doi.org/10.5194/bg-11-463-2014, 2014.
Browning, T. J., Achterberg, E. P., Rapp, I., Engel, A., Bertrand, E. M., Tagliabue, A., and Moore, C. M.: Nutrient co-limitation at the boundary of an oceanic gyre, Nature, 551, 242–246, https://doi.org/10.1038/nature24063, 2017.
Burkhardt, S., Riebesell, U., and Zondervan, I.: Effects of growth rate, CO2 concentration, and cell size on the stable carbon isotope fractionation in marine phytoplankton, Geochim. Cosmochim. Acta, 63, 3729–3741, https://doi.org/10.1016/s0016-7037(99)00217-3, 1999.
Capotondi, A., Alexander, M. A., Bond, N. A., Curchitser, E. N., and Scott, J. D.: Enhanced upper ocean stratification with climate change in the CMIP3 models, J. Geophys. Res. Oceans, 117, C04031, https://doi.org/10.1029/2011JC007409, 2012.
Cavagna, A.-J., Dehairs, F., Bouillon, S., Woule-Ebongué, V., Planchon, F., Delille, B., and Bouloubassi, I.: Water column distribution and carbon isotopic signal of cholesterol, brassicasterol and particulate organic carbon in the Atlantic sector of the Southern Ocean, Biogeosciences, 10, 2787–2801, https://doi.org/10.5194/bg-10-2787-2013, 2013.
Cheng, L., Abraham, J., Hausfather, Z., and Trenberth, K. E.: How fast are the oceans warming?, Science, 363, 128–129, https://doi.org/10.1126/science.aav7619, 2019.
Dehairs, F., Kopczynska, E., Nielsen, P., Lancelot, C., Bakker, D. C. E., Koeve, W., and Goeyens, L.: δ13C of Southern Ocean suspended organic matter during spring and early summer: regional and temporal variability, Deep-Sea Res. Pt. II, 44, 129–142, 1997.
Dickson, A. G.: Standard potential of the reaction: AgCl(s) + 0.5 H2(g) = Ag(s) + HCl(aq), and the standard acidity constant of the ion HSO in synthetic sea water from 273.15 to 318.15 K, J. Chem. Thermodyn., 22, 113–127, https://doi.org/10.1016/0021-9614(90)90074-Z, 1990.
Dickson, A. G., Afghan, J. D., and Anderson, G. C.: Reference materials for oceanic CO2 analysis: a method for the certification of total alkalinity, Mar. Chem., 80, 185–197, https://doi.org/10.1016/S0304-4203(02)00133-0, 2003.
Eppley, R. W. and Peterson, B. J.: Particulate organic-matter flux and planktonic new production in the deep ocean, Nature, 282, 677–680, 1979.
Falkowski, P. G., Ziemann, D., Kolber, Z., and Bienfang, P. K.: Role of eddy pumping in enhancing primary production in the ocean, Nature, 352, 55–58, 1991.
Fawcett, S. E., Lomas, M. W., Casey, J. R., Ward, B. B., and Sigman, D. M.: Assimilation of upwelled nitrate by small eukaryotes in the Sargasso Sea, Nat. Geosci., 4, 717, https://doi.org/10.1038/ngeo1265, 2011.
Finkel, Z. V., Beardall, J., Flynn, K. J., Quigg, A., Rees, T. A. V., and Raven, J. A.: Phytoplankton in a changing world: cell size and elemental stoichiometry, J. Plankt. Res., 32, 119–137, https://doi.org/10.1093/plankt/fbp098, 2010.
Fontugne, M. and Duplessy, J. C.: Carbon isotope ratio of marine plankton related to surface water masses, Earth Planet Sc. Lett., 41, 365–371, https://doi.org/10.1016/0012-821x(78)90191-7, 1978.
Francey, R. J., Allison, C. E., Etheridge, D. M., Trudinger, C. M., Enting, I. G., Leuenberger, M., Langenfelds, R. L., Michel, E., and Steele, L. P.: A 1000-year high precision record of delta C-13 in atmospheric CO2, Tellus B, 51, 170–193, https://doi.org/10.1034/j.1600-0889.1999.t01-1-00005.x, 1999.
Francois, R., Altabet, M. A., Goericke, R., McCorkle, D. C., Brunet, C., and Poisson, A.: Changes in the delta C-13 of surface water particulate organic matter across the subtropical convergence in the SW Indian Ocean, Global Biogeochem. Cy., 7, 627–644, https://doi.org/10.1029/93gb01277, 1993.
Freeman, K. H. and Hayes, J. M.: Fractionation of carbon isotopes by phytoplankton and estimates of ancient CO2 levels, Global Biogeochem. Cy., 6, 185–198, 1992.
Friedli, H., Lotscher, H., Oeschger, H., Siegenthaler, U., and Stauffer, B.: Ice core record of the C-13/C-12 ratio of atmospheric CO2 in the past two centuries, Nature, 324, 237–238, https://doi.org/10.1038/324237a0, 1986.
Fry, B. and Wainright, S. C.: Diatom Sources Of 13, Mar. Ecol. Prog. Ser., 76, 149–157, 1991.
Ganeshram, R. S., Calvert, S. E., Pedersen, T. F., and Cowie, G. L.: Factors controlling the burial of organic carbon in laminated and bioturbated sediments off NW Mexico: Implications for hydrocarbon preservation, Geochim. Cosmochim. Acta, 63, 1723–1734, 1999.
Gibb, S. W., Barlow, R. G., Cummings, D. G., Rees, N. W., Trees, C. C., Holligan, P., and Suggett, D.: Surface phytoplankton pig- ment distributions in the Atlantic Ocean: an assessment of basin scale variability between 50 degrees N and 50 degrees S, Prog. Oceanogr., 45, 339–368, https://doi.org/10.1016/S0079-6611(00)00007-0, 2000.
Goericke, R. and Fry, B.: Variations of marine plankton delta C-13 with latitude, temperaure and dissolved CO2 in the world ocean, Global Biogeochem. Cy., 8, 85–90, https://doi.org/10.1029/93gb03272, 1994.
Gruber, N., Keeling, C. D., Bacastow, R. B., Guenther, P. R., Lueker, T. J., Wahlen, M., Meijer, H. A. J., Mook, W. G., and Stocker, T. F., Spatiotemporal patterns of carbon-13 in the global surface oceans and the oceanic Suess effect, Global Biogeochem. Cy., 13, 307–335, https://doi.org/10.1029/1999gb900019, 1999.
Gruber, N., Clement, D., Carter, B. R., Feely, R. A., Heuven, S. van, Hoppema, M., Ishii, M., Key, R. M., Kozyr, A., Lauvset, S. K., Monaco, C. L., Mathis, J. T., Murata, A., Olsen, A., Perez, F. F., Sabine, C. L., Tanhua, T., and Wanninkhof, R.: The oceanic sink for anthropogenic CO2 from 1994 to 2007, Science, 363, 1193–1199, https://doi.org/10.1126/science.aau5153, 2019.
Hansman, R. L. and Sessions, A. L.: Measuring the in situ carbon isotopic composition of distinct marine plankton populations sorted by flow cytometry, Limnol. Oceanogr.-Methods, 14, 87–99, 2016.
Hayes, J. M., Popp, B. N., Takigiku, R., and Johnson, M. W.: An isotopic study of biogeochemical relationships between carbonates and organic carbon in the Greenhorn formation, Geochim. Cosmochim. Acta, 53, 2961–2972, https://doi.org/10.1016/0016-7037(89)90172-5, 1989.
Heimann, M. and Maier-Reimer, E.: On the relations between the oceanic uptake of CO2 and its carbon isotopes, Global Biogeochem. Cy., 10, 89–110, https://doi.org/10.1029/95GB03191, 1996.
Henley, S. F., Annett, A. L., Ganeshram, R. S., Carson, D. S., Weston, K., Crosta, X., Tait, A., Dougans, J., Fallick, A. E., and Clarke, A.: Factors influencing the stable carbon isotopic composition of suspended and sinking organic matter in the coastal Antarctic sea ice environment, Biogeosciences, 9, 1137–1157, https://doi.org/10.5194/bg-9-1137-2012, 2012.
Henson, S. A., Cole, H. S., Hopkins, J., Martin, A. P., and Yool, A.: Detection of climate change-driven trends in phytoplankton phenology, Glob. Change Biol., 24, 101–111, https://doi.org/10.1111/gcb.13886, 2018.
Hofmann, M., Wolf-Gladrow, D. A., Takahashi, T., Sutherland, S. C., Six, K. D., and Maier-Reimer, E.: Stable carbon isotope distribution of particulate organic matter in the ocean: a model study, Mar. Chem., 72, 131–150, https://doi.org/10.1016/s0304-4203(00)00078-5, 2000.
Humphreys, M. P.: Calculating seawater total alkalinity from open-cell titration data using a modified Gran plot technique, in: Measurements and Concepts in Marine Carbonate Chemistry (PhD Thesis, Ocean and Earth Science, University of Southampton, UK), 25–44, 2015.
Humphreys, M. P.: Climate sensitivity and the rate of ocean acidification: future impacts, and implications for experimental design, ICES J. Mar. Sci., 74, 934–940, https://doi.org/10.1093/icesjms/fsw189, 2017.
IPCC: Summary for Policymakers. In: Climate Change 2013: The Physical Science Basis, Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change, edited by: Stocker, T. F., Qin, D., Plattner, G.-K., Tignor, M., Allen, S. K., Boschung, J., Nauels, A., Xia, Y., Bex, V., and Midgley, P. M., Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA, 2013.
Irwin, A. J. and Oliver, M. J.: Are ocean deserts getting larger?, Geophys. Res. Lett., 36, L18609, https://doi.org/10.1029/2009GL039883, 2009.
Ito, T., Parekh, P., Dutkiewicz, S., and Follows, M. J.: The Antarctic Circumpolar Productivity Belt, Geophys. Res. Lett., 32, L13604, https://doi.org/10.1029/2005gl023021, 2005.
Jasper, J. P. and Gagosian, R. B.: The sources and deposition of organic matter in the late quaternary pygmy basin, Gulf of Mexico, Geochim. Cosmochim. Acta, 54, 1117–1132, 1990.
Jasper, J. P., Hayes, J. M., Mix, A. C., and Prahl, F. G.: Photosynthetic fractionation of C-13 and concentrations of dissolved CO2 in the central equatorial Pacific during the last 255,000 years, Paleoceanography, 9, 781–798, https://doi.org/10.1029/94pa02116, 1994.
Keeling, C. D.: The Suess effect: 13Carbon-14Carbon interrelations, Environ. Int., 2, 229–300, https://doi.org/10.1016/0160-4120(79)90005-9, 1979.
Khatiwala, S., Tanhua, T., Mikaloff Fletcher, S., Gerber, M., Doney, S. C., Graven, H. D., Gruber, N., McKinley, G. A., Murata, A., Ríos, A. F., and Sabine, C. L.: Global ocean storage of anthropogenic carbon, Biogeosciences, 10, 2169–2191, https://doi.org/10.5194/bg-10-2169-2013, 2013.
Latasa, M. and Bidigare, R. R.: A comparison of phytoplankton populations of the Arabian Sea during the Spring Intermonsoon and Southwest Monsoon of 1995 as described by HPLC-analyzed pigments, Deep-Sea Res. Pt. I, 45, 2133–2170, 1998.
Laws, E. A., Popp, B. N., Bidigare, R. R., Kennicutt, M. C., and Macko, S. A., Dependence of phytoplankton carbo isotopic composition on growth rate and CO2(aq) – Theoretical considerations and experimental results, Geochim. Cosmochim. Acta, 59, 1131–1138, https://doi.org/10.1016/0016-7037(95)00030-4, 1995.
Lee, K., Kim, T.-W., Byrne, R. H., Millero, F. J., Feely, R. A., and Liu, Y.-M.: The universal ratio of boron to chlorinity for the North Pacific and North Atlantic oceans, Geochim. Cosmochim. Acta, 74, 1801–1811, https://doi.org/10.1016/j.gca.2009.12.027, 2010.
Le Quere, C., Aumont, O., Bopp, L., Bousquet, P., Ciais, P., Francey, R., Heimann, M., Keeling, C. D., Keeling, R. F., Kheshgi, H., Peylin, P., Piper, S. C., Prentice, I. C., and Rayner, P. J.: Two decades of ocean CO2 sink and variability, Tellus B, 55, 649–656, 2003.
Lewis, E. and Wallace, D. W. R.: Program Developed for CO2 System Calculations. ORNL/CDIAC-105, Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, TN, USA, 1998.
Lindell, D. and Post, A. F.: Ultraphytoplankton succession is triggered by deep winter mixing in the Gulf of Aqaba (Eilat), Red Sea, Limnol. Oceanogr., 40, 1130–1141, https://doi.org/10.4319/lo.19188.8.131.520, 1995.
Longhurst, A. R.: Ecological Geography of the Sea, Academic Press, San Diego, 1998.
Lourey, M. J., Trull, T. W., and Tilbrook, B.: Sensitivity of delta C-13 of Southern Ocean suspended and sinking organic matter to temperature, nutrient utilization, and atmospheric CO2, Deep-Sea Res. Pt. I, 51, 281–305, https://doi.org/10.1016/j.dsr.2003.10.002, 2004.
Maranon, E., Lorenzo, M. P., Cermeno, P., and Mourino-Carballido, B.: Nutrient limitation suppresses the temperature dependence of phytoplankton metabolic rates, Isme Journal, 12, 1836–1845, https://doi.org/10.1038/s41396-018-0105-1, 2018.
McNeil, B. I., Matear, R. J., and Tilbrook, B.: Does carbon 13 track anthropogenic CO2 in the Southern Ocean?, Global Biogeochem. Cy., 15, 597–613, https://doi.org/10.1029/2000GB001352, 2001.
Mehrbach, C., Culberson, C. H., Hawley, J. E., and Pytkowicz, R. M.: Measurement of the Apparent Dissociation Constants of Carbonic Acid in Seawater at Atmospheric Pressure, Limnol. Oceanogr., 18, 897–907, https://doi.org/10.4319/lo.1973.18.6.0897, 1973.
Middelburg, J. J.: Stable isotopes dissect aquatic food webs from the top to the bottom, Biogeosciences, 11, 2357–2371, https://doi.org/10.5194/bg-11-2357-2014, 2014.
Minagawa, M. and Wada, E.: Stepwise Enrichment of N-15 Along Food-Chains – further Evidence and the Relation between DeltaN-15 and Animal Age, Geochim. Cosmochim. Acta, 48, 1135–1140, https://doi.org/10.1016/0016-7037(84)90204-7, 1984.
Pancost, R. D., Freeman, K. H., Wakeham, S. G., and Robertson, C. Y.: Controls on carbon isotope fractionation by diatoms in the Peru upwelling region, Geochim. Cosmochim. Acta, 61, 4983–4991, 1997.
Peterson, B. J. and Fry, B.: Stable isotopes in ecosystem studies, Annu. Rev. Ecol. Syst., 18, 293–320, 1987.
Popp, B. N., Laws, E. A., Bidigare, R. R., Dore, J. E., Hanson, K. L., and Wakeham, S. G.: Effect of phytoplankton cell geometry on carbon isotopic fractionation, Geochim. Cosmochim. Acta, 62, 69–77, https://doi.org/10.1016/s0016-7037(97)00333-5, 1998.
Popp, B. N., Trull, T., Kenig, F., Wakeham, S. G., Rust, T. M., Tilbrook, B., Griffiths, B., Wright, S. W., Marchant, H. J., Bidigare, R. R., and Laws, E. A.: Controls on the carbon isotopic composition of Southern Ocean phytoplankton, Global Biogeochem. Cy., 13, 827–843, https://doi.org/10.1029/1999gb900041, 1999.
Quay, P., Sonnerup, R., Westby, T., Stutsman, J., and McNichol, A.: Changes in the C-13/C-12 of dissolved inorganic carbon in the ocean as a tracer of anthropogenic CO2 uptake, Global Biogeochem. Cy., 17, 4-1–4-20, https://doi.org/10.1029/2001gb001817, 2003.
Rau, G. H., Takahashi, T., and Marais, D. J. D., Latitudinal variations in plankton delta C-13 – implications for CO2 and productivity in past oceans, Nature, 341, 516–518, https://doi.org/10.1038/341516a0, 1989.
Rau, G. H., Teyssie, J. L., Rassoulzadegan, F., and Fowler, S. W.: C-13/C-12 and N-15/N-14 variations among size-fractionated marine particles – implications for their origin and trophic relationships, Mar. Ecol. Prog. Ser., 59, 33–38, 1990.
Rau, G. H., Froelich, P. N., Takahashi, T., and Des Marais, D. J.: Does sedimentary organic d13C record variations in quaternary ocean CO2aq?, Paleoceanography, 6, 335–347, https://doi.org/10.1029/91pa00321, 1991.
Rau, G. H., Riebesell, U., and Wolf-Gladrow, D.: A model of photosynthetic C-13 fractionation by marine phytoplankton based on diffusive molecular CO2 uptake, Mar. Ecol. Prog. Ser., 133, 275–285, https://doi.org/10.3354/meps133275, 1996.
Raven, J. A., Cockell, C. S., and La Rocha, C. L.: The evolution of inorganic carbon concentrating mechanisms in photosynthesis, Philos. T. Roy. Soc. B, 363, 2641–2650, https://doi.org/10.1098/rstb.2008.0020, 2008.
Rousseaux, C. S. and Gregg, W. W.: Recent decadal trends in global phytoplankton composition, Global Biogeochem. Cy., 29, 1674–1688, https://doi.org/10.1002/2015GB005139, 2015.
Sabine, C. L. and Tanhua, T.: Estimation of Anthropogenic CO2 Inventories in the Ocean, Ann. Rev. Mar. Sci., 2, 175–198,, https://doi.org/10.1146/annurev-marine-120308-080947, 2010.
Sackett, W. M.: The depositional history and isotopic organic carbon composition of marine sediments, Mar. Geol., 2, 173–185, 1964.
Sackett, W. M., Eckelmann, W. R., Bender, M. L., and Be, A. W. H.: Temperature dependence of carbon isotope composition in marine plankton and sediments, Science, 148, 235, https://doi.org/10.1126/science.148.3667.235, 1965.
Sarmiento, J. L., Slater, R., Barber, R., Bopp, L., Doney, S. C., Hirst, A. C., Kleypas, J., Matear, R., Mikolajewicz, U., Monfray, P., Soldatov, V., Spall, S. A., and Stouffer, R.: Response of ocean ecosystems to climate warming, Global Biogeochem. Cy., 18, GB3003, https://doi.org/10.1029/2003gb002134, 2004.
Schlitzer, R.: Ocean Data View, available at: https://odv.awi.de/ (last access: 11 July 2019), 2018.
Sharkey, T. D., Berry, J. A., and Raschke, K.: Starch and sucrose synthesis in phaeseolus-vulgaris as affected by light, CO2 and abscisic acid, Plant Physiol. 77, 617–620, 1985.
Siegel, D. A., Maritorena, S., Nelson, N. B., Behrenfeld, M. J., and McClain, C. R.: Colored dissolved organic matter and its influence on the satellite-based characterization of the ocean biosphere, Geophys. Res. Lett., 32, L20605, https://doi.org/10.1029/2005gl024310, 2005.
Tuerena, R. E., Ganeshram, R. S., Geibert, W. Fallick, A. E., Dougans, J., Tait, A., Henley, S. F., and Woodward, E. M. S.: Nutrient cycling in the Atlantic basin: The evolution of nitrate isotope signatures in water masses, Global Biogeochem. Cy., 29, 1830–1844, https://doi.org/10.1002/2015GB005164, 2015.
Uitz, J., Huot, Y., Bruyant, F., Babin, M., and Claustre, H.: Relating phytoplankton photophysiological properties to community structure on large scales, Limnol. Oceanogr., 53, 614–630, https://doi.org/10.4319/lo.2008.53.2.0614, 2008.
van Heuven, S., Pierrot, D., Rae, J. W. B., Lewis, E., and Wallace, D. W. R.: CO2SYS v 1.1, MATLAB program developed for CO2 system calculations. ORNL/CDIAC-105b, Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, U.S. Department of Energy, Oak Ridge, TN, USA, https://doi.org/10.3334/CDIAC/otg.CO2SYS_MATLAB_v1.1, 2011.
Villinski, J. C., Dunbar, R. B., and Mucciarone, D. A.: Carbon 13 Carbon 12 ratios of sedimentary organic matter from the Ross Sea, Antarctica: A record of phytoplankton bloom dynamics, J. Geophys. Res.-Oceans, 105, 14163–14172, https://doi.org/10.1029/1999jc000309, 2000.
Weiss, R. F.: Carbon dioxide in water and seawater: the solubility of a non-ideal gas, Mar. Chem., 2, 203–215, https://doi.org/10.1016/0304-4203(74)90015-2, 1974.
Young, J. N., Bruggeman, J., Rickaby, R. E. M., Erez, J., and Conte, M.: Evidence for changes in carbon isotopic fractionation by phytoplankton between 1960 and 2010, Global Biogeochem. Cy., 27, 505–515, https://doi.org/10.1002/gbc.20045, 2013.
Zeebe, R. E., Sanyal, A., Ortiz, J. D., and Wolf-Gladrow, D. A.: A theoretical study of the kinetics of the boric acid-borate equilibrium in seawater, Mar. Chem., 73, 113–124, 2001.