Articles | Volume 21, issue 21
https://doi.org/10.5194/bg-21-4723-2024
https://doi.org/10.5194/bg-21-4723-2024
Research article
 | 
01 Nov 2024
Research article |  | 01 Nov 2024

Carbon degradation and mobilisation potentials of thawing permafrost peatlands in northern Norway inferred from laboratory incubations

Sigrid Trier Kjær, Sebastian Westermann, Nora Nedkvitne, and Peter Dörsch
Abstract

Permafrost soils are undergoing rapid thawing due to climate change and global warming. Permafrost peatlands are especially vulnerable, as they are located near the southern margin of the permafrost domain in the zones of discontinuous and sporadic permafrost. They store large quantities of carbon (C) which, upon thawing, may be decomposed and released as carbon dioxide (CO2), methane (CH4) and dissolved organic carbon (DOC). This study characterises patterns of potential C degradation and mobilisation within an area with sporadic permafrost by evaluating C degradation in three permafrost peatland ecosystems in Finnmark, Norway, under laboratory conditions. Active-layer, transition zone and permafrost samples from distinct cores were thawed under controlled conditions and incubated for up to 350 d under initially oxic or anoxic conditions while measuring CO2, CH4 and DOC production. Carbon degradation varied among the three peat plateaus but showed a similar trend over depth, with the largest CO2 production rates in the upper active layer and the top of the permafrost. Despite marked differences in peat chemistry between the layers, post-thaw CO2 production of permafrost peat throughout the first 350 d reached 67 %–125 % of that observed in samples from the top of the active layer. De novo CH4 production occurred after prolonged anoxic incubation in samples from the transition zone and permafrost, but it was not found in active-layer samples. CH4 production was highest in incubations from thermokarst peat sampled next to decaying peat plateaus. DOC production by active-layer samples throughout 350 d incubation exceeded gaseous C loss by up to 23-fold under anoxic conditions, whereas production by permafrost peat was small. Taken together, the results of our study suggest that permafrost peat in thawing Norwegian peat plateaus degrades at rates similar to those of active-layer peat, while the highest CH4 production can be expected after the inundation of thawed permafrost material in thermokarst ponds.

1 Introduction

In the Northern Hemisphere, around 15 % of the terrestrial surface is underlain by permafrost, of which 8 %–12 % is permafrost-affected peatland, covering 1.7×106 km2 (Obu et al., 2019; Hugelius et al., 2020). About one-third of the carbon (C) stored in permafrost-affected soils is contained in peatlands (Lindgren et al., 2018), amounting to  185 Pg C (Hugelius et al., 2020). In northern Scandinavia, peatlands have acted as a long-term C sink in the Holocene (Panneer Selvam et al., 2017). Rapid global warming, particularly in Arctic regions, is expected to cause permafrost thawing and destabilise carbon, leading to a net release of C into the atmosphere in the form of carbon dioxide (CO2) and methane (CH4) (Ramage et al., 2024; Wang et al., 2022) or to water courses in the form of dissolved organic carbon (DOC). Both CO2 and CH4 are greenhouse gases that contribute to global warming, and permafrost thawing may thus amplify global warming (Knoblauch et al., 2018).

Peatlands in northern Norway are located in the sporadic permafrost zone, forming peat plateaus and palsas, i.e. peat uplands and mounds with a frozen core lifted above the water table through formation of segregation ice (Alewell et al., 2011). Peat plateaus in northern Norway decreased in lateral extent by 33 %–71 % from 1950 to 2010, with the largest change recorded in the last decade (Borge et al., 2017). In permafrost peatlands, thawing often occurs abruptly by thermal erosion due to excess ice melt (Martin et al., 2021), exposing thawing permafrost peat to anoxia when inundated in thermokarst ponds. Environmental factors controlling the degradation of organic matter from thawing peat plateaus are still poorly understood. Thermokarst ponds are the natural succession to peat plateaus after thawing and are thus crucial for understanding future permafrost degradation and climate feedback. Thermokarst ponds accumulate new peat over time, eventually forming non-permafrost peatlands (Clymo and Hayward, 1982). It is unclear whether the climate-change-driven transformation from peat plateaus to thermokarst and non-permafrost peatlands results in an overall increase in C storage or whether the system is turned into a net C source (Turetsky et al., 2007; Treat et al., 2015, 2021). Understanding the mechanisms and extent of C degradation in thawing peat permafrost is therefore crucial for predicting future Arctic C balances.

In this study, we characterised the post-thaw degradation kinetics of peat from three Norwegian permafrost peatlands. The peat plateaus were selected to represent an area with sporadic permafrost differing with respect to (1) peat and permafrost age and (2) climatic conditions. We incubated samples from vertical peat profiles, ranging from the surface through the active layer to the mineral layer below the permafrost peat, and evaluated the relative C degradation potentials of permafrost peat differing with respect to age and decomposition history. We used both short- and long-term incubations at moderate temperature (10 °C): short-term incubations to explore the immediate metabolic response with and without oxygen (O2), as little is known about the resuscitation kinetics of microbial decomposers after controlled thawing of permafrost peat, and long-term incubation to evaluate differences in the degradability of active-layer and permafrost peat, which has been shown to differ greatly with O2 availability and temperature (Kirkwood et al., 2021; Treat et al., 2014; Waldrop et al., 2021; Panneer Selvam et al., 2017). Permafrost peat differs distinctly with respect to O2 availability between the active layer and the permafrost, with the former being drier and more exposed to O2. However, O2 availability in the active layer varies greatly during the year, as it is frozen in winter and thaws over the summer (Åkerman and Johansson, 2008). The collapse of peat plateaus and sequential formation of thermokarst ponds also change the O2 availability and thus the degradation potentials (Hodgkins et al., 2014). To compare freshly thawed permafrost peat with thermokarst peat thawed in situ, we incubated additional samples from corresponding depth profiles in neighbouring thermokarst ponds. As depth-resolved measurements are extremely rare for permafrost peat, the goal was to explore whether depth patterns are site-specific or can be generalised across permafrost peatlands in northern Norway. The main objective of the study was to quantify the C degradation potentials of permafrost peat and its partitioning into CO2, CH4 and DOC. To study the influence of O2 availability on C partitioning, peat samples were incubated both initially oxically and throughout anoxically for up to 350 d. Additionally, to explore the role of microbial growth in C degradation and mobilisation, a parallel set of samples were incubated as stirred soil slurries for 96 d, thus eliminating diffusional constraints on substrate availability. To compare CO2 and CH4 production potentials along a thaw gradient, additional incubations were carried out with recently thawed peat material obtained from shallow thermokarst depressions adjacent to two of the peat plateaus.

2 Materials and methods

2.1 Site description

Three peat plateaus in the sporadic permafrost zone of Finnmark, northern Norway, were selected along a climatic gradient spanning from the Lakselv coastal site with a maritime climate to the more continental sites of Iškoras and Áidejávri (Table 1). Iškoras and Áidejávri are situated on Finnmarksvidda, a 22 000 km2 plateau with elevations of 300 to 500 m a.s.l. (metres above sea level). At Iškoras, the peat development started around 9200 cal yr BP (Kjellman et al., 2018), whereas the peatland age at Áidejávri is unknown. However, the general timing of peat development is likely similar to that at the Iškoras site. The peatland at the Lakselv coastal site developed around 6150 cal yr BP, after the site had emerged from the adjacent fjord due to postglacial land heave (Kjellman et al., 2018). Permafrost formation for Iškoras started during the Little Ice Age, with radiocarbon dates from a single core suggesting permafrost formation at around 800 cal yr BP, whereas formation occurred later for Lakselv, at around 150 cal yr BP (Kjellman et al., 2018). The peatlands at Lakselv are situated under the marine limit, and the total-column-integrated C content is smaller than at Iškoras (Kjellman et al., 2018). The dominant vegetation on the peat plateaus has been characterised as dwarf shrubs, mosses, lichen and cloudberry herbs at Iškoras and Lakselv (Kjellman et al., 2018; Martin et al., 2019). Thermokarst and surrounding wet fen areas are dominated by sedges, cotton grass and Sphagnum sp. (Kjellman et al., 2018; Martin et al., 2019). Áidejávri has similar vegetation; however, this site has not been investigated and described in detail. The soils at the three peat plateaus are characterised as Histosols (IUSS Working Group WRB 2014). Typical bulk densities of peat plateaus in the region range from 0.08 to 0.28 g cm−3 in the active-layer and permafrost peat, with low values below the active layer due to high excess ice contents and low values in the top of the active layer due to high porosity in the surface peat (Kjellman et al., 2018). This is also reflected in the gravimetric water contents in our thawed samples provided in Table S11.

Table 1Location and characteristics of the three study sites. The mean annual air temperature (MAAT) and mean annual precipitation (MAP) for the period from 1991 to 2020 are from the closest meteorological station (Šihččajávri for Áidejávri, Čoavddatmohkki for Iškoras and Banak for Lakselv) and were obtained from the Klimaservicesenter (2021). Differences in altitude were corrected using a standard lapse rate of 0.65 °C per 100 m. Peat and permafrost formation dates are from Kjellman et al. (2018).

NA: not available.

Download Print Version | Download XLSX

2.2 Field sampling

Intact cores from the permafrost-underlain peat plateaus were collected in September 2020 at the time of maximum active-layer depth. The active layer was sampled using a cutting tool and a small shovel. Each core was divided into three depth layers – denoted as active layer (AL), transition zone (TZ, i.e. the top of the frozen layer, which is likely to thaw occasionally) and permafrost (PF) – as shown in Fig. 1. For two of the three cores, a fourth (mineral) layer was sampled below the peat. All active-layer samples were kept cool until placed in a refrigerator at 3.8 °C (SD = 0.47 °C) in the laboratory. At the time of sampling, the active-layer depth was 0.6 m at Iškoras and Lakselv and 0.5 m at Áidejávri. The frozen TZ and PF cores were sampled using a steel pipe (outside diameter 38 mm, inside diameter 30 mm) that was hammered vertically in  5 cm increments into the frozen peat. A paper towel was pushed through the pipe between each sample, and tools used for handling samples were wiped with disinfectant to minimise cross-contamination between samples. Incremental coring was continued until the mineral soil below the peat was reached. The total thickness of the organic core (active and frozen peat layers) was 1.67 m at Iškoras, 1.04 m at Áidejávri and 0.85 m at Lakselv. Subsamples of the core ( 5 cm in length) were transferred to 50 mL centrifuge tubes, which were immediately sealed by a screw cap and placed into a cooling box that was kept below 0 °C until it was transferred to a 20 °C freezer on the same day. The samples were shipped frozen to the laboratory, where they were kept at 17.8 °C (SD = 0.39 °C). To facilitate comparison between permafrost cores from different sites, the samples were assigned to seven operational layers according to their vertical structure, consisting of (from top to bottom) three samples from the active layer (AL1, AL2 and AL3), one from the transition zone (TZ) and three from the permafrost layer (PF1, PF2 and PF3). The active-layer samples were assigned upon visual inspection. AL1 and AL3 were sampled from the top and bottom of the active layer, respectively, and AL2 was sampled from the middle. AL1 did not contain surface vegetation but was less decomposed than AL2 and AL3. The TZ sample was taken from the top of the frozen core, and the PF1 sample was taken from just below this. PF3 was taken from the bottom of the frozen core, which consisted of mineral soil at Áidejávri and Lakselv. PF2 samples were taken in between PF1 and PF3. The absolute depth of the operational layers differed across the three permafrost cores (Table S1).

https://bg.copernicus.org/articles/21/4723/2024/bg-21-4723-2024-f01

Figure 1Schematic drawing of permafrost and thermokarst core. The solid green line indicates the surface, while the dashed green line indicates growth of new peat. The solid blue line shows the approximate position of the permafrost table, while the dashed blue line is the maximum thaw depth that can occasionally be thawed. The abbreviations used in the figure are as follows: AL – active layer; TZ – transition zone; PF – permafrost; TK – thermokarst; NP – new peat (i.e. accumulated following thaw, only at Áidejávri).

Download

To study the C degradation potential of recently thawed and inundated peat material, thawed peat material from thermokarst depressions adjacent to the peat plateaus was sampled in September 2021 and incubated for 96 d (see below). Using orthophotos from annual drone overflights, the time of thawing was estimated to 2017 or 2018 for Iškoras (Fig. 2; similar to Martin et al., 2021) and to before 2003 (from earliest available orthophoto; Fig. 3; Norgeibilder, 2023) for Áidejávri. Visual inspection of the thermokarst cores allowed one to distinguish the more decomposed former active-layer peat (TK-AL) from the former permafrost peat (TK-PF1 and TK-PF2/3) below (Fig. 1) so that samples corresponding to the layers distinguished in the permafrost cores (Table S2) could be taken. At Iškoras, the former peat plateau surface was submerged under 20 cm of standing water, while a fresh peat layer had formed on top of the submerged peat plateau surface at the Áidejávri site.

https://bg.copernicus.org/articles/21/4723/2024/bg-21-4723-2024-f02

Figure 2Aerial images of the Iškoras sampling site obtained by drone (processing as in Martin et al., 2021). The top panel presents a 2015 orthophoto from a drone survey (scene width 100 m), with red dots showing the locations of peat plateau (PP) and thermokarst (TK) cores sampled in 2020 and 2021, respectively. The bottom four panels present magnified orthophotos of the area defined by the black rectangle in the top panel for September 2015, 2015, 2017 and 2018, respectively; these images show the initially intact peat plateau edge collapsing in a shallow thermokarst pond from where thermokarst samples were taken in 2020.

https://bg.copernicus.org/articles/21/4723/2024/bg-21-4723-2024-f03

Figure 3Aerial images of the Áidejávri sampling site: (a) 2003 aerial image by the Norwegian Mapping Authority (Norgeibilder, 2023); (b) 2018 orthophoto from a drone survey (processing as in Martin et al., 2021); (c) magnification of the black rectangle in panel (b), with red dots showing the locations of peat plateau (PP) and thermokarst (TK) cores. The white arrow in panels (a) and (b) points to the drained thermokarst pond visible in both 2003 and 2018 imagery. The scene width in panels (a) and (b) is 130 m.

2.3 Stable isotope signatures and elemental analysis

Elemental composition was analysed on freeze-dried, homogenised samples using inductively coupled plasma mass spectrometry (ICP-MS; 8800 ICPMS Triple Quad, Agilent Technologies) and inductively coupled plasma optical emission spectroscopy (ICP-OES; 5110 ICPOES, Agilent Technologies). First, 0.20–0.25 g of the homogenised, freeze-dried material was weighed in acid-washed Teflon tubes. Each tube was filled with 2 mL of ultrapure dH2O and 5 mL of ultrapure concentrated HNO3. Samples were incubated overnight to ensure moisturisation, before decomposition in an ultraclave (ultraCLAVE, Milestone). The decomposed sample was then added to a fresh 50 mL centrifugation vial, amended with 20 mL of ultrapure dH2O and 1 mL of concentrated HCl before filling up the tube to 50 mL with ultrapure dH2O. The tubes were shaken 10 times to ensure mixing. Traceability and accuracy of the elemental analysis were ensured by including standards of known elemental composition (NCS ZC73013, NCS DC73349, Peach Leaves-1547, Pine Needles-1575 and River Sediment-LGC6187).

Contents of C and N and their natural 13C and 15N abundances were analysed using a flash combustion elemental analyser (FlashEA 1112 HT, Thermo Fisher Scientific) coupled through a ConFlow3 interface to an isotope ratio mass spectrometer (DeltaplusXP, Thermo Finnigan). Isotope values were calibrated against certified reference materials (IAEA-N1 and IAEA-CO8) and are expressed in delta (δ) notation relative to Vienna Peedee Belemnite (VPDB) and atmospheric N2, respectively. To compare the C content with the organic matter content, loss on ignition was measured at 550 °C ± 25 with samples dried at 60 °C overnight.

2.4 Incubation set-up

Incubations were set up in batches, studying one complete core of each site at a time without technical replication. Each permafrost core was divided into seven layers (Fig. 1 and Table S1) and the thermokarst core was divided into three or four layers (Table S2) from which four samples each were prepared. Each sample was divided into four portions which were placed in 120 mL serum bottles and capped with crimp-sealed butyl septa. Frozen core samples (from the TZ and PF layers) were divided lengthwise while frozen to minimise gas release. To remove the gases released during sample preparation and to minimise exposure to O2 during thawing, the bottles were placed on ice and washed with helium 6.0 (He) using an automated manifold, alternately evacuating and filling the headspace seven times. Helium overpressure was removed before placing the bottles into a temperature-controlled cabinet at 3.8 °C for overnight thawing. Headspace gas concentrations were determined after  20 h of thawing.

After controlled thawing in He and measuring gas release, the four replicate bottles for each sample were assigned to different treatments: two bottles were kept as “loose peat” at natural moisture content (Table S11), while the peat in the other two bottles was dispersed in 52 mL of ultra-distilled water (3.8 °C) by magnetic stirring, creating “peat slurries”.

The slurries were stirred for 1 h to fully disperse the peat, whereafter the peat material was allowed to settle, and 2 mL of supernatant was sampled with a syringe to measure pH and DOC concentration. pH was measured using a HACH H170 pH meter, before centrifuging the aliquot at 10 000 g for 10 min and filtering it through a 0.45 µm filter (sterile syringe filter with polyethersulfone membrane, VWR International) for analysing water-extractable DOC by a total organic carbon analyser (TOC-V, Shimadzu). Hereafter, one bottle of each set (loose and slurry) was washed with He, as described above, to create anaerobic conditions, while the other two were washed with a He / O2 (80/20) mixture to create initially aerobic conditions. A major goal of this study was to explore the depth dependency of peat mineralisation rates. Given the constraints of the laboratory set-up (e.g. number of bottles which could be incubated simultaneously) and the limited sample volume available per depth, we did not combine the samples of bulk layers (e.g. the entire active layer) and perform technical replicates (as in e.g. Kirkwood et al., 2021; Treat et al., 2014). Instead, we retained the high depth resolution and performed only a single incubation per depth and treatment. While this can lead to higher uncertainty in individual incubation results, we generally base our findings on a number of incubated samples (e.g. full vertical depth profiles), thus strongly moderating the influence of single biased measurements.

2.5 Incubation experiments

All bottles, both the loose and the stirred peat suspensions, were incubated in a temperature-controlled water bath at 10 °C using an incubator with automated gas analysis (Molstad et al., 2007, 2016). The incubator consists of a temperature-controlled water bath with submersible stirring boards holding up to 44 serum bottles (120 mL) and is placed under the robotic arm of an autosampler (GC-Pal, CTC) which repeatedly pierces the septum of the bottles with a hypodermic needle and pumps  1 mL via a peristaltic pump (Gilson 222XL) to a multi-column, multi-detector gas chromatograph (GC; Agilent 7890A) equipped with an automatic sample admission system. Upon injection, the peristaltic pump is reversed, and sample gas not injected onto the columns is pumped back to the bottle along with He, maintaining the pressure in the bottles at  1 atm. The GC has two columns, a PoraPLOT Q column to separate CH4, CO2 and N2O from bulk air and a Molesieve column to separate O2+ Ar from N2, and three detectors (TCD, FID and ECD) for simultaneously determining O2, CO2, N2, CH4 and N2O concentrations. Dry bottles with standard mixtures of known concentrations (AGA, Norway) were included in the measurement sequence for calibration and for evaluating the dilution resulting from back-pumping He after each sampling. He-filled bottles were included to evaluate the leakage of O2 into the measurement system. After converting peak areas to parts per million by volume (ppmv), moles of CO2 and CH4 accumulated or O2 consumed were calculated considering the dissolution in peat water (Wilhelm et al., 1977; Appelo and Postma, 1993), dilution by He back-pumping and leakage of O2 during sample admission. For more details, the reader is referred to Molstad et al. (2007).

Headspace gas concentrations were monitored every 4.5 h for 413 to 450 h ( 17 to 19 d) for permafrost cores and 180 h ( 7 d) for thermokarst cores to investigate post-thaw gas kinetics at high resolution. Thereafter, the bottles were transferred to a temperature-controlled cabinet adjusted to 9.7 °C (SD = 0.04), where the incubations were continued without stirring. The slurries were shaken at least once a week, after which headspace samples were retrieved manually for offline gas chromatography. After about 1 month, the measurement frequency was decreased to biweekly until 96 d of incubation, upon which the incubation of slurries and thermokarst samples was discontinued. Loosely packed permafrost core samples continued incubation at 10 °C until 350 d after thawing, and headspace concentrations were sporadically measured during this time (after  10.5 months and  1 year).

To evaluate microbial growth from initial high-resolution gas kinetics, we fitted selected periods of exponential product accumulation to a growth model (Eq. 1) following Stenström et al. (1998, 2001):

(1) p = p 0 + r μ ( e μ t - 1 ) + K t ,

where p is the product concentration, p0 is the product concentration at time 0, r is the total respiration rate, μ is the specific growth rate, t is the time and K is a constant respiration rate for non-growing microorganisms. Before fitting the curves, we truncated CO2 kinetics from the first 17 to 19 d of incubation, excluding the transitional phase of decreasing accumulation rates towards the stationary phase. SigmaPlot 14.0 was used to fit Eq. (1) to the selected data, and significant (p<0.05) specific growth rates (μ) are reported. A Student t test with two-tailed distribution and two-sample unequal variance was used to evaluate differences in geochemical peat characteristics among the sites using Microsoft Excel for Microsoft 365.

3 Results

3.1 Peat characteristics

The peat plateau at Iškoras featured the highest C content among the three studied permafrost cores, with little variation throughout the peat profile (491–552 mg C g dw−1, where “dw” denotes dry weight; Fig. 4). Permafrost cores from Áidejávri and Lakselv had similar C contents in the active layer (439–551 mg C g dw−1) but held less C in the permafrost. Organic matter (OM) content mirrored C content, except for the deep permafrost (PF3) at Lakselv, which indicated a high concentration of inorganic C in this layer (Fig. 4). Other layers showed no indication of inorganic C, and C contents are henceforth referred to as organic C (OC). The permafrost core from Iškoras had the highest C/N ratios irrespective of depth, with the highest ratio in the top active layer (AL1) gradually decreasing with depth. Áidejávri and Lakselv also featured the highest C/N ratios in AL1, below which they decreased more strongly throughout the active layer, while being stable throughout the permafrost zone. DOC extracted 20 h after thawing was highest in samples from the transition zone and from the top of the permafrost zone, with values at Iškoras clearly exceeding those at Áidejávri and Lakselv (p<0.019). pH increased with depth in all three cores and was significantly lower (p<0.009) at Iškoras compared with the other two cores. Lakselv was most depleted in 13C, whereas Iškoras and Áidejávri showed more variable δ13C values with depth. As for δ13C, the δ15N values were highest in the top layer (AL1) and decreased throughout the active layer, decreasing the most at Iškoras and the least at Lakselv. Áidejávri and Lakselv featured the highest iron (Fe) contents, while Iškoras had almost no Fe throughout the profile. Phosphorus (P) content was quite even throughout the profiles at all sites, while Lakselv had a higher P content than the other two sites. Sulfur (S) content increased in the TZ and PF layers at Iškoras and Áidejávri, while it was more evenly distributed with depth at Lakselv (Fig. 4).

https://bg.copernicus.org/articles/21/4723/2024/bg-21-4723-2024-f04

Figure 4Depth profiles of geochemical variables in permafrost cores at Iškoras, Áidejávri and Lakselv. Shown are (from left to right) carbon content (mg C g dw−1), organic matter content (mg OM g dw−1), carbon-to-nitrogen ratio (C/N), dissolved organic carbon (mg DOC g dw−1), pH, isotope signatures of carbon (δ13C) and nitrogen (δ15N), iron content (mg Fe g dw−1), phosphorous content (mg P g dw−1), and sulfur content (mg S g dw−1). The deepest layer at Áidejávri and Lakselv was affected by mineral soils. The thaw depths at the coring location were 60 cm (Iškoras and Lakselv) and 50 cm (Áidejávri).

Download

3.2 Gas kinetics

The initial gas formation throughout the first 20 d revealed clear kinetic differences between sites and depths in permafrost cores, as showcased in Fig. 5 and Table 2. CO2 production kinetics were most dynamic for Iškoras (TZ), showing exponential product accumulation during the initial incubation (0–19 d) of permafrost samples, under both oxic and anoxic conditions (e.g. insert in Fig. 5a and Table 2). A similar pattern was observed for samples from Áidejávri and Lakselv (Fig. 5a and Table 2). By contrast, all AL samples incubated loosely showed linear CO2 accumulation (Table 2).

https://bg.copernicus.org/articles/21/4723/2024/bg-21-4723-2024-f05

Figure 5Kinetics of CO2 and CH4 release from permafrost core samples (loosely packed) from layers with the highest CH4 production at Iškoras (TZ), Áidejávri (PF1) and Lakselv (PF2). Initial data for Lakselv are missing due to instrument failure. (a) CO2 accumulation of initially oxic peat throughout 350 d with a fitted hyperbola (f=ax/(b+x)). (b) CH4 accumulation of anoxically peat throughout 350 d fitted to Eq. (1).

Download

When incubating samples as stirred slurries, two effects were observed: (1) some of the AL samples turned to exponential CO2 accumulation and (2) there was a tendency toward greater apparent microbial growth, as indicated by specific growth rates, μ, in Table 2, except for anoxically incubated samples from Áidejávri and Lakselv. Estimated specific growth rates ranged from 0.07 to 0.007 h−1, corresponding to generation times of 14 to 145 h. CO2 production at all sites and depths showed a tendency to level off over time (Fig. 5a).

Except for Áidejávri, CH4 accumulation during the first 20 d was small and curvilinear, and rates in samples from Lakselv and Áidejávri did not increase before  100 d of incubation. During the first 96 d of the incubation, all Iškoras PF and TZ samples displayed CH4 kinetics as shown in Fig. 5b, with apparent initial CH4 production levelling off over time irrespective of whether the samples were incubated stirred/unstirred or oxically/anoxically (Fig. S1), suggesting that initial CH4 release was not due to methanogenesis. This was further supported by the higher initial CH4 release from Iškoras samples compared with Áidejávri samples during the first 20 h of thawing (Table S6). In contrast, TZ, PF1 and PF2 samples from Áidejávri accumulated CH4 exponentially throughout the first 19 d, indicating methanogenesis (Figs. 5b and S1). After levelling off, CH4 production increased in PF and TZ samples from Áidejávri after about 80 d and continued throughout the remainder of the incubation. Samples from Lakselv featured less CH4 production at the beginning of the incubation; however, production increased greatly towards the end of the incubation, resulting in greater final CH4 accumulation than in samples from Áidejávri or Iškoras (Fig. 5b). N2O kinetics (production and uptake) were observed, but accumulated amounts were minute and are not reported here.

Table 2Specific growth rates (h−1) derived from the exponential part of high-resolution CO2 kinetics during initial incubation (0–19 d). All rates are statistically significant at p<0.05. Averages are calculated for TZ and PF samples ± SD.

Download Print Version | Download XLSX

3.3 Cumulative CO2 production

Expressed as cumulative CO2 production over 96 d, the AL1 layers of all three permafrost cores showed the highest CO2 production values under oxic conditions (Fig. 6). Cumulative CO2 production decreased strongly with depth throughout the active layer, before increasing again in the TZ layer and reaching a secondary maximum in the upper permafrost layer. Maximum CO2 production of PF peat after 96 d accounted for 42, 102 and 60 % of the CO2 production observed in the top of the active layer (AL1) at Iškoras (PF1), Áidejávri (PF1) and Lakselv (PF2), respectively, indicating that oxic post-thaw respiration of permafrost peat can reach values comparable to those of the surface layer with fresh litter input. Across the three sites, PF peat at Lakselv accumulated the least CO2 (Fig. 6). Incubating initially aerobic samples beyond 96 d revealed a gradual decrease in CO2 production in all layers of all sites (Fig. 5a), which coincided with O2 depletion and, in some cases, even O2 exhaustion (Figs. S4, S6 and S8). Notwithstanding, the AL and PF top samples of the initially oxic treatment showed the largest relative increments in cumulative CO2 production from 96 to 350 d (Fig. 6). After 350 d, CO2 accumulation of PF samples from Iškoras (PF1) reached 67 % of the CO2 accumulation in the AL1 sample, Áidejávri (PF1) reached 125 % and Lakselv (PF2) reached 72 %. CO2 production was greatly reduced in the absence of O2, irrespective of depth and incubation time (Fig. 6).

https://bg.copernicus.org/articles/21/4723/2024/bg-21-4723-2024-f06

Figure 6Depth profiles of cumulative CO2 production throughout 96 and 350 d of incubation as loosely packed permafrost core samples for (a) Iškoras, (b) Áidejávri and (c) Lakselv. The depth indicates the average depth of the incubated sample. Stippled lines indicate the thaw depth at sampling and the location of the transition zone (TZ). Anoxic PF2 for Áidejávri could not be measured after 96 d due to leakage.

Download

In general, there was little effect of stirring on the decomposition during the first 96 d (Fig. S3), indicating that peat degradation was minimally controlled by matrix effects or diffusional constraints. However, due to the addition of water and the associated decease in headspace volume, there was less O2 available in slurried samples than in loosely packed peat, which likely shortened the period of oxic respiration (Figs. S4–S9).

CO2 production in thermokarst cores (Iškoras and Áidejávri) was of the same order of magnitude (40 to 241 µmol CO2 g dw−1 96 d−1) as that in the permafrost cores, but it was exceeded by CO2 production by new “peat” at Áidejávri (616 µmol CO2 g dw−1 96 d−1) (Table S3).

3.4 Methane release and production

Significant CH4 production was only observed in TZ and PF samples, despite prolonged anoxic incubation of samples from all depths (Fig. 7). Methane production after 96 d was up to 4 orders of magnitude smaller than CO2 production on a C basis. However, while CO2 production slowed down over time, CH4 production increased (Fig. 5b), indicating that methanogenesis in thawing PF peat had a lag phase before producing CH4 (Knoblauch et al., 2018). Over 350 d, the anoxic CH4-C accumulation at Lakselv (PF2) reached 38.6 % of its CO2-C accumulation, while the corresponding values for Áidejávri (PF1) and Iškoras (TZ) were 9.2 % and 0.4 %, respectively.

https://bg.copernicus.org/articles/21/4723/2024/bg-21-4723-2024-f07

Figure 7Depth profiles of cumulative CH4 production in permafrost cores from Iškoras, Áidejávri and Lakselv incubated anoxically as loosely packed samples for (a) 96 d and (b) 350 d. CH4 was corrected for desorption by subtracting CH4 accumulating in oxic bottles from anoxic CH4 release to obtain biogenic CH4 production (Fig. S2). Anoxic PF2 from Áidejávri could not be measured after 350 d due to leakage. The active-layer depths at the coring locations were 60 cm (Iškoras and Lakselv) and 50 cm (Áidejávri).

Download

As for CO2, suspending and initially stirring the peat slurry had little effect on cumulative CH4 production within the first 96 d (Fig. S2). However, during the initial high-resolution gas measurements, CH4 production in PF1 from Áidejávri, the only layer showing biogenic CH4 production during initial anoxic incubation, was inhibited by stirring. High-resolution CH4 kinetics of the non-stirred sample were markedly sigmoid (Fig. S1), suggesting that stirring may inhibit growth of methanogens.

CH4 production in thermokarst samples differed markedly from that of the corresponding layers in the permafrost cores (Table S4). At Iškoras, CH4 production in thermokarst cores was between 2 and 4 orders of magnitude larger than in corresponding samples from the intact permafrost core after 96 d. Differences between thermokarst and corresponding permafrost layers were somewhat smaller for Áidejávri, although still pronounced (Table S4). At both sites, CH4 production was highest in the top layer of the thermokarst core (3 to 4 orders of magnitude larger than that in AL1 from the permafrost core), while CH4 production in TK-PF1 and TK-PF2/3 at Áidejávri was of the same order of magnitude (Table S4). In general, thermokarst samples responded more to O2 and stirring/non-stirring than samples from the intact permafrost core, which makes it difficult to interpret differences between depths and sites (Figs. S11 and S12). Nevertheless, the potential to produce CH4 increased dramatically in the former active-layer peat when inundated (TK-AL) for both sites (Table S4), illustrating the strongly increased methanogenetic potential of peat plateau AL peat after thermokarst formation.

3.5 Total C mobilisation

Net release of DOC was measured as the difference between the initial and final extractable DOC. It greatly exceeded CO2-C release in loosely packed active-layer samples (Fig. 8). Initially oxically incubated samples (Fig. 8a) had markedly smaller DOC values but higher CO2-C production than anoxically incubated samples (Fig. 8b) and vice versa. pH increased more in anoxic incubations (Table S10) and might have made DOC more easily extractable. When combining net DOC release/uptake and CO2-C production, total C mobilisation was highest in the active layer, irrespective of initial O2 status (Fig. 8). AL2 from Áidejávri showed exceptionally high C mobilisation compared with Iškoras and Lakselv. TZ and PF samples showed a tendency toward net DOC consumption, which was most pronounced in samples from Iškoras and matched high apparent specific growth rates there (Table 2).

https://bg.copernicus.org/articles/21/4723/2024/bg-21-4723-2024-f08

Figure 8Stacked CO2-C production and net DOC release/uptake throughout  350 d of incubation at 10 °C. Data shown are from loosely packed permafrost core samples (a) incubated initially oxically and (b) anoxically. * No CO2 data available due to leakage.

Download

4 Discussion

The cumulative CO2 and CH4 production of three permafrost cores showed a distinct depth pattern (Figs. 6 and 7). The highest CO2 production was found in the top of the active layer, in the transition zone and in the top of the permafrost (Fig. 6). This suggests that thawed permafrost peat from Norwegian peat plateaus has a considerable potential for CO2 production, comparable to that of active layers. By contrast, CH4 production under anoxic conditions was almost exclusively found in samples from thawed permafrost layers (Fig. 7). So far, only a few studies have investigated decomposition in decaying peat plateaus by comparing post-thaw CO2 production in thawed permafrost peat with that of overlaying active layers (Treat et al., 2014; Waldrop et al., 2021; Kirkwood et al., 2021; Harris et al., 2023).

Although caution is warranted when comparing decomposition rates across different incubation studies, Kirkwood et al. (2021) found, similarly to our study, the highest CO2 release in the top of the active layer for Canadian peat plateaus (see the supplementary material of Kirkwood et al., 2021) and, for some sites, a second maximum in the permafrost peat. To compare the decomposition dynamics of the active-layer and permafrost peat in our study with those of Kirkwood et al. (2021), we calculated average rates for the active layer and permafrost (Table S7). The comparison reveals a similar trend in both studies, with the active layer having higher degradation potentials under anoxic conditions than PF layers. The rates of anoxic CO2 accumulation in active-layer and permafrost peat reported by Kirkwood et al. (2021) were similar to the rates that we found for Lakselv after adjustment for temperature and length of incubation (Table S7). Both Iškoras and Áidejávri featured higher CO2 production rates in the active-layer and permafrost peat (Table S7) than the averages reported by Kirkwood et al. (2021). This could indicate a higher C degradability at our sites in northern Norway compared with the Canadian sites, but this should be corroborated by more detailed studies including technical replicates of the incubated samples.

By contrast, Treat et al. (2014) and Waldrop et al. (2021) did not observe differences in C degradation between different depths in Alaskan peat plateaus. However, these studies differed from our study with respect to peat stratigraphy. Furthermore, the degradation potentials were higher than those found in the present study (Tables S8 and S9). Harris et al. (2023) found that C degradation in Canadian peat plateaus was highest in the top of the active layer and that deeper layers had low CO2 production throughout. These variations could be due to differences in C quality among the different sites or due to differences in sample treatment.

In general, incubation conditions differ across published studies, and information about peat formation and quality is often lacking, making it difficult to compare C degradation rates. Our study demonstrates the variability in the potential degradation rates within the same geographical region, suggesting that more studies are needed to estimate potential climate feedback of permafrost peat thaw at regional scales. A standardised incubation protocol would improve the comparability among studies and help to validate and improve numerical models of C dynamics in permafrost peatlands (e.g. Treat et al., 2021), which are critical tools to quantify present and future climate change impacts on these sensitive ecosystems.

4.1 Constraints and variability in C decomposition

Oxygen availability appeared to be the most important factor for C decomposition. CO2 accumulation after 350 d in anoxic incubations reached only 7 % to 61 % of that in initially oxic incubations (Table S12). This is in agreement with other studies which found that O2 availability is critical for CO2 production after permafrost thaw (Estop-Aragones et al., 2018; Schädel et al., 2016). The incubation study by Waldrop et al. (2021) using Arctic Alaskan peat found that CO2 accumulation after 6 months at 5 °C under anoxic conditions accounted for 26 % of that measured under oxic conditions. Moreover, initially oxic conditions did not appear to increase subsequent anoxic C degradation.

Incubating the samples as stirred slurries versus loosely placed peat affected initial gas kinetics. We observed exponential CO2 accumulation within the first 20 d of incubation in some of the bottles, indicating microbial growth (Fig. 5a and Table 2). In loosely packed samples, this phenomenon was only observed in permafrost samples but not in active-layer samples. As expected, active-layer samples showed exponential CO2 accumulation only when stirred, likely because stirring increases substrate availability. Together, this might suggest that microbial growth is a constitute part of the post-thaw resuscitation response in permafrost peat. Fitting the accumulation curves to a mixed growth model produced realistic specific growth rates (Eq. 1). It has to be noted, however, that the estimated specific growth rates after thawing had no repercussions for long-term decomposition; plotting cumulative CO2 production after 350 d over µ did not reveal any significant relationship. The largest amount of decomposition was observed with initial µ values  0.03 (data not shown).

Constantly stirred slurries were included as a treatment to explore the effect of relieving diffusional constraints for O2 and substrates during aerobic and anaerobic metabolism. As expected, constant stirring of slurries increased CO2 production and O2 consumption in the first phase of the incubation. However, the addition of water to the slurries decreased the headspace volume; this meant that the absolute amount of O2 present in the oxic slurries was smaller than in the oxic incubations with loose peat, which biases the comparison between loosely paced and slurried oxic incubation (Figs. S4–S9). In general, differences in cumulative CO2 production between loosely packed samples and slurries were small (Fig. S3).

The finding that CO2 production of the TZ and PF samples was comparable to that of active-layer samples suggests that C quality does not limit microbial decomposition of permafrost peat after thawing, despite it having been frozen for centuries (Fig. 6). This was true for all three sites, despite marked variations in peat decomposability among the sites. The common depth pattern of degradability could be related to a similar formation history of the three studied peat plateaus (Table 1). The top of the active layer, where the highest rates were measured, includes the root zone with continuous input of fresh plant litter (Fig. 6). This was also reflected in high C/N and δ13C values in this layer at all three sites (Fig. 4). In the lower parts of the active layer, the peat has likely been exposed to aerobic conditions for decades or even centuries, without input of fresh plant litter. It may, therefore, be strongly decomposed and, thus, depleted of labile C, which would explain the lower observed degradation rates for the AL2 and AL3 samples (Fig. 6). On the other hand, the PF layers contain frozen peat produced under wetland (i.e. mostly anaerobic) conditions, which means that it has not been exposed to decomposition yet. Here, more labile C may be available, which could explain the secondary peak in degradation rates observed for the TZ and PF1 samples (Fig. 6). Another explanation of why C degradation varied over depth at Áidejávri and Lakselv could be the increasing content of iron over depth (Fig. 4), as iron can trap organic C and limit mobilisation and degradation (Patzner et al., 2020). However, this cannot explain C degradation at Iškoras, as this site generally has very little iron (Fig. 4). Differences in C decomposition potentials between the sites might also be explained by differences in the formation history or site-specific environmental factors. A study on a Swedish peat plateau found that the reductive dissolution of iron during permafrost thaw can lead to an increase in CH4 emissions (Patzner et al., 2022). This might explain why Lakselv and Áidejávri, which both have high iron contents, showed a faster increase in CH4 production compared with Iškoras (Figs. 4 and 7).

4.2 Total C mobilisation

Initially oxic samples from all sites did not produce CH4 for 350 d even after turning anoxic, indicating that methanogenesis was inhibited beyond the depletion of O2 (Figs. S4, S6 and S8). However, it cannot be ruled out that longer incubation could have resulted in CH4 production (Knoblauch et al., 2018). DOC was consumed to a larger degree in initially oxic samples compared with anoxic PF samples (Fig. 8). This may be partly due to less DOC degradation under anoxic conditions; however, it could also be related to the higher extractability of DOC at a higher pH in anoxic samples (Table S10).

In incubations of thawed cores from a Finnish peat plateau, Panneer Selvam et al. (2017) found that DOC from the active layer had a lower initial degradation potential than DOC from thawed permafrost. This agrees with our finding that CO2 accumulation in permafrost samples was faster than in deeper active-layer samples despite releasing less DOC, suggesting that “old” permafrost DOC was more degradable than “new” DOC in the active layer (Figs. 6 and 8). This trend was especially evident at Áidejávri, where CO2 accumulation in the top permafrost exceeded that of the top active layer, despite releasing more DOC in the active layer. Still, the substantial release of DOC from the active layer is of concern because DOC is easily lost along water paths and can potentially increase the CO2 production downstream (Panneer Selvam et al., 2017; Voigt et al., 2019). The DOC run-off from permafrost-affected areas has been found to both increase C emission from fresh waters and contribute a significant amount of C export to the Arctic Ocean (Vorobyev et al., 2021).

Differences in gas kinetics for both CO2 and CH4 among the three peat plateaus (Fig. 5) could be related to differences in the abundance and taxonomic composition of microbial communities. We tried to work aseptically during field sampling and laboratory sample handling, but cross-contamination (e.g. by the corer) cannot be ruled out. Notwithstanding, our results suggest that permafrost samples harbour competent microbial taxa that proliferate over time, as seen by the exponential product accumulation in some of our incubations. An incubation study with permafrost soil from the Tibetan Plateau found that CO2 release after thawing was positively related to functional gene abundance for C degradation (Chen et al., 2020), without involving changes in the taxonomic composition. In general, high-altitude ecosystems differ from high-latitude ecosystems with respect to having lower OM and ice contents, with consequences for microbial community composition (Wang and Xue, 2021). In the present study, there were clear differences in the kinetics of gaseous product accumulation, for both CO2 and CH4 (Fig. 5 and Table 2). Thawed permafrost peat from Iškoras supported exponential CO2 accumulation, whereas PF peat from Áidejávri supported exponential CH4 accumulation, strongly indicating microbial growth which will eventually result in community change. Detailed molecular studies would be needed to elucidate whether post-thaw community change leads to more degradation of permafrost C overall or to a shift in the CO2/ CH4 ratio under anoxic conditions.

4.3 Thermokarst peat decomposition

Inundation in thermokarst may be the ultimate fate of permafrost peat from thawing peat plateaus. It involves mixing with unfrozen peat, extended anoxia when inundated and buried by sediments, and access to fresh C from autotrophic production in the ponds. Our results showed that the potential CO2 production of permafrost peat thawed in situ in a thermokarst pond (TK-PF1) was lower than of permafrost peat thawed ex situ (PF), although still of the same order of magnitude (Table S3). On the other hand, CH4 production potentials over 96 d were several orders of magnitude higher in thermokarst samples than in undisturbed PF samples (Table S4). This may be due to the proliferation of a highly productive methanogenetic community over time as well as additional nutrient input from surrounding non-PF fens or bogs (in 't Zandt et al., 2020).

The observed increase in CH4 production between 96 and 350 d of incubation for permafrost samples (Fig. 7), especially those from Lakselv and Áidejávri, suggests that the proliferation of a functioning methanogenetic community after permafrost thaw takes time and depends, among others, on the fate of permafrost peat (e.g. inundation) after peat plateau collapse. This could mean that CH4 production measured ex situ greatly underestimates the true CH4 production potential of in situ thawed material, even in longer-term incubations. A similar conclusion can be drawn from the findings of Knoblauch et al. (2013), who incubated permafrost material from Holocene and Late Pleistocene permafrost sediments of the Lena River delta in Siberia and found that CH4 production reached maximum rates after an average of 2.6 years of incubation.

In our study, most CH4 was produced by thermokarst peat from Iškoras (Table S4); this could be related to the fact that permafrost thaw occurred more recently, with labile C still being present. The Áidejávri thermokarst site, on the other hand, has been thawed for a longer time; this might explain why the C is more stable, resulting in less methanogenesis. Another explanation might be differences in environmental factors such as soil moisture, temperature and vegetation composition (Olefeldt et al., 2013).

Similar to our study, Kirkwood et al. (2021) incubated both peat plateau and thermokarst peat anoxically and found higher CH4 emissions in the thermokarst compared with the active-layer and permafrost samples after 225 d (Table S7). High pH was found to be a good predictor of potential CH4 production in the Canadian thermokarst. In our work, the opposite was the case: CH4 production was highest in Iškoras thermokarst with a lower pH compared with in Áidejávri (Tables S4 and S5). This suggests that local differences in peat quality and time since thawing play a role in the CH4 emission potential.

5 Conclusions

This study evaluates the C degradability from the active layer, transition zone and permafrost at three peat plateau sites in northern Norway through ex situ incubations. In addition, samples from thermokarst adjacent to the peat plateaus were investigated at two of the sites. Observed C degradation rates varied among the three sites, while all three sites showed similar degradation patterns over depth, with the highest CO2 production in the top of the active layer and a second maximum in the permafrost layers. High-resolution post-thaw gas kinetics showed marked differences in microbial growth response; however, this did not affect long-term C mineralisation potentials. The main limitation for C degradation was O2 availability. Significant CH4 production was only observed in samples from the transition zone and permafrost layers after prolonged anoxic incubation. CH4 production increased over time, showing that methanogenesis could play an important role in C degradation under prolonged anoxic conditions. This was further supported by thermokarst samples that showed 2–4 orders of magnitude larger CH4 production rates compared with freshly thawed peat plateau samples. DOC released during the incubation of active-layer peat plateau samples exceeded gaseous C release. Our incubation study indicates that the burial of permafrost peat in thermokarst and DOC run-off to downstream ecosystems should be taken into account when estimating C degradation in collapsing peat plateau ecosystems.

Data availability

The data associated with this work are available from Zenodo: https://doi.org/10.5281/zenodo.10696561 (Kjær et al., 2024).

Supplement

The supplement related to this article is available online at: https://doi.org/10.5194/bg-21-4723-2024-supplement.

Author contributions

All authors contributed to the conceptualisation of the study and participated in data collection. STK, NN and PD conducted the experiments and performed data analysis. STK and SW created the figures. STK drafted the initial manuscript, and STK, SW and PD revised and edited the final version.

Competing interests

The contact author has declared that none of the authors has any competing interests.

Disclaimer

Publisher's note: Copernicus Publications remains neutral with regard to jurisdictional claims made in the text, published maps, institutional affiliations, or any other geographical representation in this paper. While Copernicus Publications makes every effort to include appropriate place names, the final responsibility lies with the authors.

Acknowledgements

We would like to thank the laboratory staff at the Faculty of Environmental Sciences and Natural Resource Management, NMBU, especially Trygve Fredriksen, Pia Frostad and Solfrid Lohne.

Review statement

This paper was edited by Bertrand Guenet and reviewed by two anonymous referees.

References

Åkerman, H. J. and Johansson, M.: Thawing permafrost and thicker active layers in sub-arctic Sweden, Permafrost Periglac., 19, 279–292, https://doi.org/10.1002/ppp.626, 2008. 

Alewell, C., Giesler, R., Klaminder, J., Leifeld, J., and Rollog, M.: Stable carbon isotopes as indicators for environmental change in palsa peats, Biogeosciences, 8, 1769–1778, https://doi.org/10.5194/bg-8-1769-2011, 2011. 

Appelo C. A. J. and Postma, D.: Geochemistry, groundwater and pollution, A. A. Balkema/Rotterdam, 536 pp., ISBN 90 5410 105 9, 1993. 

Borge, A. F., Westermann, S., Solheim, I., and Etzelmüller, B.: Strong degradation of palsas and peat plateaus in northern Norway during the last 60 years, The Cryosphere, 11, 1–16, https://doi.org/10.5194/tc-11-1-2017, 2017. 

Chen, Y., Liu, F., Kang, L., Zhang, D., Kou, D., Mao, C., Qin, S., Zhang, Q., and Yang, Y.: Large-scale evidence for microbial response and associated carbon release after permafrost thaw, Glob. Change Biol., 27, 3218–3229, https://doi.org/10.1111/gcb.15487, 2020. 

Clymo, R. S. and Hayward, P. M.: The Ecology of Sphagnum, edited by: Smith, A. J. E., Bryophyte Ecology, Springer, Dordrecht, https://doi.org/10.1007/978-94-009-5891-3_8, 1982. 

Estop-Aragones, C., Cooper, M. D. A., Fisher, J. P., Thierry, A., Garnett, M. H., Charman, D. J., Murton, J. B., Phoenix, G. K., Treharne, R., Sanderson, N. K., Burn, C. R., Kokelj, S. V., Wolfe, S. A., Lewkowicz, A. G., Williams, M., and Hartley, I. P.: Limited release of previously-frozen C and increased new peat formation after thaw in permafrost peatlands, Soil Biol. Biochem., 118, 115–129, https://doi.org/10.1016/j.soilbio.2017.12.010, 2018.  

Harris, L. I., Olefeldt, D., Pelletier, N., Blodau, C., Knorr, K.-H., Talbot, J., Heffernan, L., and Turetsky, M.: Permafrost thaw causes large carbon loss in boreal peatlands while changes to peat quality are limited, Glob. Change Biol., 29, 5720–5735, https://doi.org/10.1111/gcb.16894, 2023. 

Hodgkins, S. B., Tfaily, M. M., McCalley, C. K., Logan, T. A., Crill, P. M., Saleska, S. R., Rich, V. I., and Chanton, J. P.: Changes in peat chemistry associated with permafrost thaw increase greenhouse gas production, P. Natl. Acad. Sci. USA, 111, 5819–5824, https://doi.org/10.1073/pnas.1314641111, 2014. 

Hugelius, G., Loisel, J., Chadburn, S., Jackson, R. B., Jones, M., MacDonald, G., Marushchak, M., Olefeldt, D., Packalen, M., Siewert, M. B., Treat, C., Turetsky, M., Voigt, C., and Yu, Z.: Large stocks of peatland carbon and nitrogen are vulnerable to permafrost thaw, P. Natl. Acad. Sci. USA, 117, 20438–20446, https://doi.org/10.1073/pnas.1916387117, 2020. 

in 't Zandt, M. H., Liebner, S., and Welte, C. U.: Roles of Thermokarst Lakes in a Warming World, Trend. Microbiol., 28, 769–779, https://doi.org/10.1016/j.tim.2020.04.002, 2020. 

IUSS Working Group WRB: World Reference Base for Soil Resources 2014, update 2015, International Soil Classification System for Naming Soils and Creating Legends for Soil Maps, World Soil Resources Reports 106, FAO, Rome, E-ISBN 978-92-5-108370-3, 2014. 

Kjær, S. T., Dörsch, P., Westermann, S., and Nedkvitne, N.: Permafrost peatlands: gas emission and elemental analysis, Zenodo [data set], https://doi.org/10.5281/zenodo.10696561, 2024. 

Kirkwood, J. A. H., Roy-Léveillée, P., Mykytczuk, N., Packalen, M., McLaughlin, J., Laframboise, A., and Basiliko, N.: Soil Microbial Community Response to Permafrost Degradation in Palsa Fields of the Hudson Bay Lowlands: Implications for Greenhouse Gas Production in a Warming Climate, Global Biogeochem. Cy., 35, e2021GB006954, https://doi.org/10.1029/2021GB006954, 2021. 

Kjellman, S. E., Axelsson, P. E., Etzelmuller, B., Westermann, S., and Sannel, A. B. K.: Holocene development of subarctic permafrost peatlands in Finnmark, northern Norway, Holocene, 28, 1855–1869, https://doi.org/10.1177/0959683618798126, 2018. 

Klimaservicesenter: Norsk, https://seklima.met.no/, last access: 1 May 2021. 

Knoblauch, C., Beer, C., Sosnin, A., Wagner, D., and Pfeiffer, E.-M.: Predicting long-term carbon mineralization and trace gas production from thawing permafrost of Northeast Siberia, Glob. Change Biol., 19, 1160–1172, https://doi.org/10.1111/gcb.12116, 2013. 

Knoblauch, C., Beer, C., Liebner, S., Grigoriev, M. N., and Pfeiffer, E.: Methane production as key to the greenhouse gas budget of thawing permafrost, Nat. Clim. Change, 8, 309–312, https://doi.org/10.1038/s41558-018-0095-z, 2018. 

Lindgren, A., Hugelius, G., and Kuhry, P.: Extensive loss of past permafrost carbon but a net accumulation into present-day soils, Nature, 560, 219-222, https://doi.org/10.1038/s41586-018-0371-0, 2018. 

Martin, L. C. P., Nitzbon, J., Aas, K. S., Etzelmüller, B., Kristiansen, H., and Westermann, S.: Stability conditions of peat plateaus and palsas in northern Norway, J. Geophys. Res.-Earth, 124, 705–719, https://doi.org/10.1029/2018JF004945, 2019. 

Martin, L. C. P., Nitzbon, J., Scheer, J., Aas, K. S., Eiken, T., Langer, M., Filhol, S., Etzelmüller, B., and Westermann, S.: Lateral thermokarst patterns in permafrost peat plateaus in northern Norway, The Cryosphere, 15, 3423–3442, https://doi.org/10.5194/tc-15-3423-2021, 2021. 

Molstad, L., Dörsch, P., and Bakken, L. R.: Robotized incubation system for monitoring gases (O2, NO, N2O N2) in denitrifying cultures, J. Microbiol. Method., 71, 202–211, https://doi.org/10.1016/j.mimet.2007.08.011, 2007. 

Molstad, L., Dörsch, P., and Bakken, L.: Improved robotized incubation system for gas kinetics in batch cultures, ResearchGate, https://doi.org/10.13140/RG.2.2.30688.07680, 2016. 

Norgeibilder: Flybilder, http://norgeibilder.no, last access: 15th August 2023. 

Obu, J., Westermann, S., Bartsch, A., Berdnikov, N., Christiansen, H. H., Dashtseren, A., Delaloye, R., Elberling, B., Etzelmüller, B., Kholodov, A., Khomutov, A., Kääb, A., Leibman, M. O., Lewkowicz, A. G., Panda, S. K., Romanovsky, V., Way, R. G., Westergaard-Nielsen, A., Wu, T., Yamkhin, J., and Zou, D.: Northern Hemisphere permafrost map based on TTOP modelling for 2000–2016 at 1 km2 scale, Earth-Sci. Rev., 193, 299–316, https://doi.org/10.1016/j.earscirev.2019.04.023, 2019. 

Olefeldt, D., Turetsky, M. R., Crill, P. M., and McGuire, A. D.: Environmental and physical controls on northern terrestrial methane emissions across permafrost zones, Glob. Change Biol., 19, 589–603, https://doi.org/10.1111/gcb.12071, 2013. 

Panneer Selvam, B., Lapierre, J. F., Guillemette, F., Voigt, C., Lamprecht, R. E., Biasi, C., Christensen, T. R., Martikainen, P. J., and Berggren, M.: Degradation potentials of dissolved organic carbon (DOC) from thawed permafrost peat, Sci. Rep., 7, 45811, https://doi.org/10.1038/srep45811, 2017. 

Patzner, M. S., Mueller, C. W., Malusova, M., Baur, M., Nikeleit, V., Scholten, T., Hoeschen, C., Byrne, J. M., Borch, T., Kappler, A., and Bryce, C.: Iron mineral dissolution releases iron and associated organic carbon during permafrost thaw, Nat. Commun., 11, 6329, https://doi.org/10.1038/s41467-020-20102-6, 2020. 

Patzner, M. S., Logan, M., McKenna, A. M., Young, R. B., Zhou, Z., Joss, H., Mueller, C. W., Hoeschen, C., Scholten, T., Straub, D., Kleindienst, S., Borch, T., Kappler, A., and Bryce, C.: Microbial iron cycling during palsa hillslope collapse promotes greenhouse gas emissions before complete permafrost thaw, Commun. Earth Environ., 3, 76, https://doi.org/10.1038/s43247-022-00407-8, 2022. 

Ramage, J., Kuhn, M., Virkkala, A.-M., Voigt, C., Marushchak, M. E., Bastos, A., Biasi, C., Canadell, J. G., Ciais, P., López-Blanco, E., Natali, S. M., Olefeldt, D., Potter, S., Poulter, B., Rogers, B. M., Schuur, E. A. G., Treat, C., Turetsky, M. R., Watts, J., and Hugelius, G.: The net GHG balance and budget of the permafrost region (2000–2020) from ecosystem flux upscaling, Global Biogeochem. Cy., 38, e2023GB007953, https://doi.org/10.1029/2023GB007953, 2024. 

Schädel, C., Bader, M. K. F., Schuur, E. A. G., Biasi, C., Bracho, R., Čapek, P., De Baets, S., Diáková, K., Ernakovich, J., Estop-Aragones, C., Graham, D. E., Hartley, I. P., Iversen, C. M., Kane, E., Knoblauch, C., Lupascu, M., Martikainen, P. J., Natali, S. M., Norby, R. J., O'Donnell, J. A., Chowdhury, T. R., Šantrůčková, H., Shaver, G. R., Sloan, V. L., Treat, C. C., Turetsky, M. R., Waldrop, M. P., and Wickland, K. P.: Potential carbon emissions dominated by carbon dioxide from thawed permafrost soils, Nat. Clim. Change, 6, 950–953, https://doi.org/10.1038/nclimate3054, 2016. 

Stenström, J., Stenberg, B., and Johansson, M.: Kinetics of Substrate-Induced Respiration (SIR): Theory, Ambio, 27, 35–39, http://www.jstor.org/stable/4314682 (last access: 29 October 2024), 1998. 

Stenström, J., Svensson, K., and Johansson, M.: Reversible transition between active and dormant microbial states in soil, FEMS Microbiol. Ecol., 36, 93–104, https://doi.org/10.1111/j.1574-6941.2001.tb00829.x, 2001. 

Treat, C. C., Wollheim, W. M., Varner, R. K., Grandy, A. S., Talbot, J., and Frolking, S.: Temperature and peat type control CO2 and CH4 production in Alaskan permafrost peats, Glob. Change Biol., 20, 2674–2686, https://doi.org/10.1111/gcb.12572, 2014. 

Treat, C. C., Natali, S. M., Ernakovich, J., Iversen, C. M., Lupascu, M., McGuire, A. D., Norby, R. J., Roy Chowdhury, T., Richter, A., Šantrůčková, H., Schädel, C., Schuur, E. A. G., Sloan, V. L., Turetsky, M. R., and Waldrop, M. P.: A pan-Arctic synthesis of CH4 and CO2 production from anoxic soil incubations, Glob. Change Biol., 21, 2787–2803, https://doi.org/10.1111/gcb.12875, 2015. 

Treat, C. C., Jones, M. C., Alder, J., Sannel, A. B. K., Camill, P., and Frolking, S.: Predicted vulnerability of carbon in permafrost peatlands with future climate change and permafrost thaw in Western Canada, J. Geophys. Res.-Biogeo., 126, e2020JG005872, https://doi.org/10.1029/2020JG005872, 2021. 

Turetsky, M. R., Wieder, R. K., Vitt, D. H., Evans, R. J., and Scott, K. D.: The disappearance of relict permafrost in boreal north America: Effects on peatland carbon storage and fluxes, Glob. Change Biol., 13, 1922–1934, https://doi.org/10.1111/j.1365-2486.2007.01381.x, 2007.  

Voigt, C., Marushchak, M. E., Mastepanov, M., Lamprecht, R. E., Christensen, T. R., Dorodnikov, M., Jackowicz-Korczyński, M., Lindgren, A., Lohila, A., Nykänen, H., Oinonen, M., Oksanen, T., Palonen, V., Treat, C. C., Martikainen, P. J., and Biasi, C.: Ecosystem carbon response of an Arctic peatland to simulated permafrost thaw, Glob. Change Biol., 25, 1746–1764, https://doi.org/10.1111/gcb.14574, 2019. 

Vorobyev, S. N., Karlsson, J., Kolesnichenko, Y. Y., Korets, M. A., and Pokrovsky, O. S.: Fluvial carbon dioxide emission from the Lena River basin during the spring flood, Biogeosciences, 18, 4919–4936, https://doi.org/10.5194/bg-18-4919-2021, 2021. 

Waldrop, M. P., McFarland, J., Manies, K. L., Leewis, M. C., Blazewicz, S. J., Jones, M. C., Neumann, R. B., Keller, J. K., Cohen, L., Euskirchen, E. S., Edgar, C., Turetsky, M. R., and Cable, W. L.: Carbon Fluxes and Microbial Activities From Boreal Peatlands Experiencing Permafrost Thaw, J. Geophys. Res.-Biogeo., 126, e2020JG005869, https://doi.org/10.1029/2020JG005869, 2021. 

Wang, Y.-R., Hessen, D. O., Samset, B. H., and Stordal, F.: Evaluating global and regional land warming trends in the past decades with both MODIS and ERA5-Land land surface temperature data, Remote Sens. Environ., 280, 113181, https://doi.org/10.1016/j.rse.2022.113181, 2022. 

Wang, Y. and Xue, K.: Linkage between microbial shift and ecosystem functionality, Glob. Change Biol., 27, 3197–3199, https://doi.org/10.1111/gcb.15615, 2021. 

Wilhelm, E., Battino, R., and Wilcock, R. J.: Low-pressure solubility of gases in liquid water, Chem. Rev., 77, 219–262, https://doi.org/10.1021/cr60306a003, 1977. 

Download
Short summary
Permafrost peatlands are thawing due to climate change, releasing large quantities of carbon that degrades upon thawing and is released as CO2, CH4 or dissolved organic carbon (DOC). We incubated thawed Norwegian permafrost peat plateaus and thermokarst pond sediment found next to permafrost for up to 350 d to measure carbon loss. CO2 production was initially the highest, whereas CH4 production increased over time. The largest carbon loss was measured at the top of the peat plateau core as DOC.
Altmetrics
Final-revised paper
Preprint