Articles | Volume 22, issue 18
https://doi.org/10.5194/bg-22-4679-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-22-4679-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
The incubation history of soil samples strongly affects the occlusion of particulate organic matter
Frederick Büks
CORRESPONDING AUTHOR
Department of Soil Science, Institute of Ecology, Technische Universität Berlin, 10587 Berlin, Germany
Sabine Dumke
Department of Soil Science, Institute of Ecology, Technische Universität Berlin, 10587 Berlin, Germany
Julia König
Department of Soil Science, Institute of Ecology, Technische Universität Berlin, 10587 Berlin, Germany
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Ultrasonication with density fractionation of soils is a commonly used method to separate soil organic matter pools, which is, e.g., important to calculate carbon turnover in landscapes. It is shown that the approach that merges soil and dense solution without mixing has a low recovery rate and causes co-extraction of parts of the retained labile pool along with the intermediate pool. An alternative method with high recovery rates and no cross-contamination was recommended.
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Ultrasonication/density fractionation is a common method used to extract particulate organic matter (POM) and, recently, microplastic (MP) from soil samples. In this study, ultrasonic treatment with mechanical stress increasing from 0 to 500 J mL−1 caused comminution and a reduced recovery rate of soil-derived POMs but no such effects with MP particles. In consequence, the extraction of MP from soils is not affected by particle size and recovery rate artifacts.
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Laboratory experiments that assess microplastic (MP) impact on the terrestrial environment require information on common soil MP concentrations. We reviewed item numbers and mass concentrations recorded in 23 studies, with 223 sampling sites in total with respect to the underlying entry pathways, land uses and vicinities. Common values included amounts of up to 13 000 items kg−1 and 4.5 mg kg−1 dry soil. Based on the collected data, we identified problems in past field studies.
Frederick Büks
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Short summary
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Ultrasonication with density fractionation of soils is a commonly used method to separate soil organic matter pools, which is, e.g., important to calculate carbon turnover in landscapes. It is shown that the approach that merges soil and dense solution without mixing has a low recovery rate and causes co-extraction of parts of the retained labile pool along with the intermediate pool. An alternative method with high recovery rates and no cross-contamination was recommended.
Frederick Büks and Martin Kaupenjohann
SOIL, 8, 373–380, https://doi.org/10.5194/soil-8-373-2022, https://doi.org/10.5194/soil-8-373-2022, 2022
Short summary
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The adverse effect of microplastic (MP) on soil biota and soil structure depends on MP particle size and surface characteristics. Since weathering plays a major role in the genesis of these, it must be considered in both the analysis of environmental MP and the production of artificial MP for laboratory experiments. This work integrates recent findings on adverse effects and the genesis of its surface characteristics and discusses how to reproduce them to obtain closer-to-nature designer MP.
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Biogeosciences, 18, 159–167, https://doi.org/10.5194/bg-18-159-2021, https://doi.org/10.5194/bg-18-159-2021, 2021
Short summary
Short summary
Ultrasonication/density fractionation is a common method used to extract particulate organic matter (POM) and, recently, microplastic (MP) from soil samples. In this study, ultrasonic treatment with mechanical stress increasing from 0 to 500 J mL−1 caused comminution and a reduced recovery rate of soil-derived POMs but no such effects with MP particles. In consequence, the extraction of MP from soils is not affected by particle size and recovery rate artifacts.
Frederick Büks and Martin Kaupenjohann
SOIL, 6, 649–662, https://doi.org/10.5194/soil-6-649-2020, https://doi.org/10.5194/soil-6-649-2020, 2020
Short summary
Short summary
Laboratory experiments that assess microplastic (MP) impact on the terrestrial environment require information on common soil MP concentrations. We reviewed item numbers and mass concentrations recorded in 23 studies, with 223 sampling sites in total with respect to the underlying entry pathways, land uses and vicinities. Common values included amounts of up to 13 000 items kg−1 and 4.5 mg kg−1 dry soil. Based on the collected data, we identified problems in past field studies.
Cited articles
Almajmaie, A., Hardie, M., Acuna, T., and Birch, C.: Evaluation of methods for determining soil aggregate stability, Soil Till. Res., 167, 39–45, https://doi.org/10.1016/j.still.2016.11.003, 2017.
Amézketa, E.: Soil Aggregate Stability: A Review, J. Sustain. Agr., 14, 83–151, https://doi.org/10.1300/j064v14n02_08, 1999.
Angulo, V., Bleichrodt, R. J., Dijksterhuis, J., Erktan, A., Hefting, M. M., Kraak, B., and Kowalchuk, G. A.: Enhancement of soil aggregation and physical properties through fungal amendments under varying moisture conditions, Environ. Microbiol., 26, e16627, https://doi.org/10.1111/1462-2920.16627, 2024.
Annabi, M., Houot, S., Francou, C., Poitrenaud, M., and Bissonnais, Y. L.: Soil Aggregate Stability Improvement with Urban Composts of Different Maturities, Soil Sci. Soc. Am. J., 71, 413, https://doi.org/10.2136/sssaj2006.0161, 2007.
Bavel, C. V.: Mean weight-diameter of soil aggregates as a statistical index of aggregation, Proceedings, Soil Sci. Soc. Am., 14, 20–23, https://doi.org/10.2136/sssaj1950.036159950014000C0005x, 1950.
Birch, H. F.: The effect of soil drying on humus decomposition and nitrogen availability, Plant Soil, 10, 9–31, https://doi.org/10.1007/BF01343734, 1958.
Bossuyt, H., Denef, K., Six, J., Frey, S., Merckx, R., and Paustian, K.: Influence of microbial populations and residue quality on aggregate stability, Appl. Soil Ecol., 16, 195–208, https://doi.org/10.1016/s0929-1393(00)00116-5, 2001.
Bouajila, A. and Gallali, T.: Land use effect on soil and particulate organic carbon, and aggregate stability in some soils in Tunisia, Afr. J. Agr. Res., 5, 764–774, 2010.
Bronick, C. J. and Lal, R.: Soil structure and management: a review, Geoderma, 124, 3–22, https://doi.org/10.1016/j.geoderma.2004.03.005, 2005.
Bryant, J. C., Bendixen, T. W., and Slater, C. S.: Measurement of the water-stability of soils, Soil Sci., 65, 341–346, 1948.
Büks, F. and Kaupenjohann, M.: Enzymatic biofilm digestion in soil aggregates facilitates the release of particulate organic matter by sonication, SOIL, 2, 499–509, https://doi.org/10.5194/soil-2-499-2016, 2016.
Büks, F., Kayser, G., Zieger, A., Lang, F., and Kaupenjohann, M.: Particles under stress: ultrasonication causes size and recovery rate artifacts with soil-derived POM but not with microplastics, Biogeosciences, 18, 159–167, https://doi.org/10.5194/bg-18-159-2021, 2021.
Büks, F.: Technical note: The recovery rate of free particulate organic matter from soil samples is strongly affected by the method of density fractionation, Biogeosciences, 20, 1529–1535, https://doi.org/10.5194/bg-20-1529-2023, 2023.
Cavael, U., Tost., P., Diehl, K., Büks, F., and Lentzsch, P.: Correlations of Soil Fungi, Soil Structure and Tree Vigour on an Apple Orchard with Replant Soil, Soil Syst., 4, 70, https://doi.org/10.3390/soilsystems4040070, 2020.
Cerli, C., Celi, L., Kalbitz, K., Guggenberger, G., and Kaiser, K.: Separation of light and heavy organic matter fractions in soil – Testing for proper density cut-off and dispersion level, Geoderma, 170, 403–416, https://doi.org/10.1016/j.geoderma.2011.10.009, 2012.
Chepil, W. and Bisal, F.: A Rotary Sieve Method for Determining the Size Distribution of Soil Clods, Soil Sci., 56, 95–100, https://doi.org/10.1097/00010694-194308000-00002, 1943.
Chowdhury, N., Marschner, P., and Burns, R.: Response of microbial activity and community structure to decreasing soil osmotic and matric potential, Plant Soil, 344, 241–254, https://doi.org/10.1007/s11104-011-0743-9, 2011.
Christensen, O.: An Index of Friability of Soils, Soil Sci., 29, 119–136, 1930.
Denef, K., Six, J., Paustian, K., and Merckx, R.: Importance of macroaggregate dynamics in controlling soil carbon stabilization: short-term effects of physical disturbance induced by dry–wet cycles, Soil Biol. Biochem., 33, 2145–2153, https://doi.org/10.1016/S0038-0717(01)00153-5, 2001.
DIN 19683-16:2015-12: Soil quality - Physical laboratory tests - Part 16: Determination of aggregate stability using the method of wet sieving, https://doi.org/10.31030/2360384, 2015.
Drenovsky, R. E., Vo, D., Graham, K. J., and Scow, K. M.: Soil water content and organic carbon availability are major determinants of soil microbial community composition, Microb. Ecol., 48, 424–430, https://doi.org/10.1007/s00248-003-1063-2, 2004.
Edwards, A. and Bremner, J.: Dispersion of Soil Particles by Sonic Vibration, J. Soil Sci., 18, 47–63, https://doi.org/10.1111/j.1365-2389.1967.tb01487.x, 1967a.
Edwards, A. P. and Bremner, J.: Microaggregates in Soils, J. Soil Sci., 18, 64–73, https://doi.org/10.1111/j.1365-2389.1967.tb01488.x, 1967b.
Emerson, W. W.: A classification of soil aggregates based on their coherence in water, Soil Res., 5, 47–57, https://doi.org/10.1071/SR9670047, 1967.
Fan, X., Pan, H., Ping, Y., Jin, G., and Song, F.: The underlying mechanism of soil aggregate stability by fungi and related multiple factor: a review, Eurasian Soil Sci., 55, 242–250, https://doi.org/10.1134/S1064229322020065, 2022.
Golchin, A., Oades, J. M., Skjemstad, J. O., and Clarke, P.: Study of free and occluded particulate organic matter in soils by solid state 13C CP/MAS NMR spectroscopy and scanning electron microscopy, Soil Res., 32, 285–309, https://doi.org/10.1071/SR9940285, 1994.
Graf-Rosenfellner, M., Cierjacks, A., Kleinschmit, B., and Lang, F.: Soil formation and its implications for stabilization of soil organic matter in the riparian zone, Catena, 139, 9–18, https://doi.org/10.1016/j.catena.2015.11.010, 2016.
Haynes, R. J. and Swift, R. S.: Stability of soil aggregates in relation to organic constituents and soil water content, J. Soil Sci., 41, 73–83, https://doi.org/10.1111/j.1365-2389.1990.tb00046.x, 1990.
Jouquet, P., Chintakunta, S., Bottinelli, N., Subramanian, S., and Caner, L.: The influence of fungus-growing termites on soil macro and micro-aggregates stability varies with soil type, Appl. Soil Ecol., 101, 117–123, https://doi.org/10.1016/j.apsoil.2016.02.001, 2016.
Kaiser, M. and Berhe, A. A.: How does sonication affect the mineral and organic constituents of soil aggregates? – A review, J. Plant. Nutr. Soil Sc., 177, 479–495, https://doi.org/10.1002/jpln.201300339, 2014.
Kaiser, M., Kleber, M., and Berhe, A. A.: How air-drying and rewetting modify soil organic matter characteristics: an assessment to improve data interpretation and inference, Soil Biol. Biochem., 80, 324–340, https://doi.org/10.1016/j.soilbio.2014.10.018, 2015.
Kemper, W. D. and Rosenau, R. C.: Aggregate stability and size distribution, in: Methods of soil analysis: Part 1 Physical and mineralogical methods, 5, 425–442, https://doi.org/10.2136/sssabookser5.1.2ed.c17, 1986.
Kim, N., Zabaloy, M. C., Guan, K., and Villamil, M. B.: Do cover crops benefit soil microbiome? A meta-analysis of current research, Soil Biol. Biochem., 142, 107701, https://doi.org/10.1016/j.soilbio.2019.107701, 2020.
Kühnel, A., Wiesmeier, M., Spörlein, P., Schilling, B., and Kögel-Knabner, I.: Influence of drying vs. freezing of archived soil samples on soil organic matter fractions, J. Plant Nutr. Soil Sci., 182, 772–781, https://doi.org/10.1002/jpln.201800529, 2019.
Lehtinen, T., Lair, G. J., Mentler, A., Gísladóttir, G., Ragnarsdóttir, K. V., and Blum, W. E.: Soil aggregate stability in different soil orders quantified by low dispersive ultrasonic energy levels, Soil Sci. Soc. Am. J., 78, 713–723, https://doi.org/10.2136/sssaj2013.02.0073, 2014.
Liu, J., Hu, F., Xu, C., Wang, Z., Ma, R., Zhao, S., and Liu, G.: Comparison of different methods for assessing effects of soil interparticle forces on aggregate stability, Geoderma, 385, 114834, https://doi.org/10.1016/j.geoderma.2020.114834, 2021.
Liu, J., Yang, Y., Zhou, J., Feng, X., Li, Y., Li, Y., Zi, J., Wang, C., Wang, E., and Jia, Y.: Evaluation of Soil Aggregate Sieving: The Impact of Field Moisture Content on Size Distribution and Stability, Agronomy, 15, 558, https://doi.org/10.3390/agronomy15030558, 2025.
Martínez-Mena, M., Williams, A. G., Ternan, J. L., and Fitzjohn, C.: Role of antecedent soil water content on aggregates stability in a semi-arid environment, Soil Till. Res., 48, 71–80, https://doi.org/10.1016/S0167-1987(98)00131-7, 1998.
McDaniel, M. D. and Grandy, A. S.: Soil microbial biomass and function are altered by 12 years of crop rotation, SOIL, 2, 583–599, https://doi.org/10.5194/soil-2-583-2016, 2016.
Meidl, P., Lehmann, A., Bi, M., Breitenreiter, C., Benkrama, J., Li, E., Riedo, J., and Rillig, M. C.: Combined application of up to ten pesticides decreases key soil processes, Environ. Sci. Pollut. Res., 31, 11995–12004, https://doi.org/10.1007/s11356-024-31836-x, 2024.
Mikha, M. M., Rice, C. W., and Milliken, G. A.: Carbon and nitrogen mineralization as affected by drying and wetting cycles, Soil Biol. Biochem., 37, 339–347, https://doi.org/10.1016/j.soilbio.2004.08.003, 2005.
North, P.: Towards an absolute measurement of soil structural stability using ultrasound, J. Soil Sci., 27, 451–459, https://doi.org/10.1111/j.1365-2389.1976.tb02014.x, 1976.
Oztas, T. and Fayetorbay, F.: Effect of freezing and thawing processes on soil aggregate stability, CATENA, 52, 1–8, https://doi.org/10.1016/s0341-8162(02)00177-7, 2003.
Rengasamy, P. and Olsson, K. A.: Sodicity and soil structure, Soil Res., 29, 935–952, https://doi.org/10.1071/SR9910935, 1991.
Rosenberg, N. J.: Response of plants to the physical effects of soil compaction, Adv. Agron., 16, 181–196, https://doi.org/10.1016/S0065-2113(08)60024-3, 1964.
Russell, J.: Report of the subcommittee on soil structure and consistency, Soil Sci. Soc. Am. J., 9, 10–22, https://doi.org/10.2136/sssaj1928.0361599500B920010002x, 1928.
Russell, M. B. and Feng, C. L.: Characterization of the stability of soil aggregates, Soil Sci., 63, 299–304, 1947.
Schroeder, J., Kammann, L., Helfrich, M., Tebbe, C. C., and Poeplau, C.: Impact of common sample pre-treatments on key soil microbial properties, Soil Biol. Biochem., 160, 108321, https://doi.org/10.1016/j.soilbio.2021.108321, 2021.
Tang, J., Mo, Y., Zhang, J., and Zhang, R.: Influence of biological aggregating agents associated with microbial population on soil aggregate stability, Appl. Soil Ecol., 47, 153–159, https://doi.org/10.1016/j.apsoil.2011.01.001, 2011.
Tian, J., Wu, X., Li, J., Guo, M., Zhang, X., and Chen, Q.: Seasonal and Long-Term Variability in Soil Structure and Erodibility under Different Land-Use Patterns in the Mollisols Region of Northeast China, Agronomy, 13, 449, https://doi.org/10.3390/agronomy13020449, 2023.
Tisdall, J. M., Smith, S. E., and Rengasamy, P.: Aggregation of soil by fungal hyphae, Soil Res., 35, 55–60, https://doi.org/10.1071/S96065, 1997.
Veum, K. S., Goyne, K. W., Kremer, R., and Motavalli, P. P.: Relationships among water stable aggregates and organic matter fractions under conservation management, Soil. Sci. Soc. Am. J., 76, 2143–2153, https://doi.org/10.2136/sssaj2012.0089, 2012.
Vrána, M., Kubát, J. F., Kavka, P., and Zumr, D.: A laser diffractometry technique for determining the soil water stable aggregates index, Geoderma, 441, 116756, https://doi.org/10.1016/j.geoderma.2023.116756, 2024.
Witzgall, K., Vidal, A., Schubert, D. I., Höschen, C., Schweizer, S. A., Buegger, F., Pouteau, V., Chenu, C., and Mueller, C. W.: Particulate organic matter as a functional soil component for persistent soil organic carbon. Nat. Commun., 12, 4115, https://doi.org/10.1038/s41467-021-24192-8, 2021.
Yoder, R. E.: A direct method of aggregate analysis of soils and a study of the physical nature of erosion losses, Agron. J., 28, 337–351, https://doi.org/10.2134/agronj1936.00021962002800050001x, 1936.
Short summary
Ultrasonication followed by density fractionation is a frequently used method to determine soil structural stability and the amount of occluded particulate organic matter. Our analyses of three soils (sandy, silty and loamy) showed that air drying and gentle rewetting change SOM (soil organic matter) fractions depending on the subsequent time of re-incubation compared to field-fresh samples. This is important, since, e.g., the measurement of archived soils requires the handling of air-dried samples.
Ultrasonication followed by density fractionation is a frequently used method to determine soil...
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