Preprints
https://doi.org/10.5194/bg-2021-146
https://doi.org/10.5194/bg-2021-146

  08 Jul 2021

08 Jul 2021

Review status: this discussion paper is a preprint. It has been under review for the journal Biogeosciences (BG). The manuscript was not accepted for further review after discussion.

Manifestations and environmental implications of microbially-induced calcium carbonate precipitation (MICP) by the cyanobacterium Dolichospermum flosaquae

Refat Abdel-Basset1, Elhagag Ahmed Hasssan1, and Hans-Peter Grossart2,3 Refat Abdel-Basset et al.
  • 1Botany and Microbiology Department, Faculty of Science, Assiut University, 71516 Assiut (Egypt)
  • 2Dept. Experimental Limnology, Leibniz Institute for Freshwater Ecology and Inland Fisheries, D-16775 Stechlin, Germany
  • 3Dept. of Biochemistry and Biology, Potsdam University, 14469 Potsdam, Germany

Abstract. The aim of this work is to explore the ability and magnitude of the temperate cyanobacterium Dolichospermum flosaquae in microbially-induced calcium carbonate precipitation (MICP). Environmentally, MICP controls the availability of calcium, carbon and phosphorus in freshwater lakes and simultaneously controls carbon exchange with the atmosphere. Cultures of flosaquae were grown in BG11 medium containing 0, 1, 1.5, 2 and 4 mg Ca2+ L−1, as cardinal concentrations previously reported in freshwater lakes, in addition to a control culture (BG11 containing 13 mg Ca2+ L−1). Growth (cell number, chlorophyll a, and protein content) of D. flosaquae was generally reduced by elevating calcium concentrations of the different salts used (chloride, acetate, or citrate). D. flosaquae exhibited its ability to perform MICP as carbonate alkalinity was sharply increased up to its highest level (six times that of the control) at a citrate concentration of 4 mg Ca2+ L−1. Calcium carbonate was formed at a pre-precipitation stage as the minimum pH necessary for precipitation (8.7) has been scarcely approached under such conditions. In this work, MICP took place mostly empowered by photosynthesis and respiration. Residual calcium exhibited its lowest value at 4 mg Ca2+ citrate L−1, coinciding with the highest alkalinity level. Precipitated calcium was increased with chlorophyll a content, but not with increasing cell numbers.

Refat Abdel-Basset et al.

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on bg-2021-146', O.S. Pokrovsky, 02 Sep 2021
    • AC1: 'Reply on RC1', Refat Mostafa, 27 Sep 2021
  • RC2: 'Comment on bg-2021-146', Anonymous Referee #2, 05 Sep 2021
    • AC2: 'Reply on RC2', Refat Mostafa, 27 Sep 2021

Status: closed

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on bg-2021-146', O.S. Pokrovsky, 02 Sep 2021
    • AC1: 'Reply on RC1', Refat Mostafa, 27 Sep 2021
  • RC2: 'Comment on bg-2021-146', Anonymous Referee #2, 05 Sep 2021
    • AC2: 'Reply on RC2', Refat Mostafa, 27 Sep 2021

Refat Abdel-Basset et al.

Refat Abdel-Basset et al.

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Short summary
The aim of this work is to explore the ability of the cyanobacterium Dolichospermum flosaquae in microbially-induced calcium carbonate precipitation (MICP). Environmentally, MICP controls the availability of calcium, carbon and phosphorus in freshwater lakes and carbon exchange with the atmosphere. Citrate, at 4 mg/L, induced the highest carbonate alkalinity, the highest calcium consumption, the highest urease activity along with the lowest photosynthetic and respiratory oxygen exchange.
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