Articles | Volume 11, issue 20
Biogeosciences, 11, 5847–5863, 2014

Special issue: KEOPS2: Kerguelen Ocean and Plateau Study 2

Biogeosciences, 11, 5847–5863, 2014

Research article 21 Oct 2014

Research article | 21 Oct 2014

Protist community composition during early phytoplankton blooms in the naturally iron-fertilized Kerguelen area (Southern Ocean)

C. Georges, S. Monchy, S. Genitsaris, and U. Christaki C. Georges et al.
  • INSU-CNRS, UMR8187 LOG, Laboratoire d'Océanologie et de Géosciences, Université du Littoral Côte d'Opale, ULCO, 32 avenue Foch, 62930 Wimereux, France

Abstract. Microbial eukaryotic community composition was examined by 18S rRNA gene tag pyrosequencing, during the early phase of spring phytoplankton blooms induced by natural iron fertilization, off Kerguelen Island in the Southern Ocean (KEOPS2 cruise). A total of 999 operational taxonomical units (OTUs), affiliated to 30 known high-level taxonomic groups, were retrieved from 16 samples collected in the upper 300 m water column. The alveolata group was the most abundant in terms of sequence number and diversity (696 OTUs). The majority of alveolata sequences were affiliated to Dinophyceae and to two major groups of marine alveolates (MALV-I and MALV-II). In the upper 180 m, only 13% of the OTUs were shared between of the fertilized stations and the reference site characterized by high-nutrient low-chlorophyll (HNLC) waters. Fungi and Cercozoa were present in iron-fertilized waters, but almost absent in the HNLC samples, while Haptophyta and Chlorophyta characterized the HNLC sample. Finally, the 300 m depth samples of all stations were differentiated by the presence of MALV-II and Radiolaria. Multivariate analysis, examining the level of similarity between different samples, showed that protistan assemblages differed significantly between the HNLC and iron-fertilized stations, but also between the diverse iron-fertilized blooms.

Final-revised paper