Biogeosciences
Biogeosciences
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Articles | Volume 12, issue 6
Articles | Volume 12, issue 6
https://doi.org/10.5194/bg-12-1865-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/bg-12-1865-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.
Articles | Volume 12, issue 6
Research article
 | 
20 Mar 2015
Research article |  | 20 Mar 2015

Multi-isotope labelling of organic matter by diffusion of 2H/18O-H2O vapour and 13C-CO2 into the leaves and its distribution within the plant

M. S. Studer, R. T. W. Siegwolf, M. Leuenberger, and S. Abiven

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Short summary
We present a new technique to label organic matter (OM) at its place of formation by the application of 13C, 18O and 2H through the gaseous phase. The label diffused into leaves was incorporated into assimilates and was detected in plant tissues. This technique can be applied in soil sciences, e.g. to trace the decomposition pathways of soil OM inputs, or in plant physiology and palaeoclimatic reconstruction, e.g. to further investigate the origin of the 18O and 2H signal in tree ring cellulose.
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