Articles | Volume 12, issue 6
Biogeosciences, 12, 1865–1879, 2015
Biogeosciences, 12, 1865–1879, 2015
Research article
20 Mar 2015
Research article | 20 Mar 2015

Multi-isotope labelling of organic matter by diffusion of 2H/18O-H2O vapour and 13C-CO2 into the leaves and its distribution within the plant

M. S. Studer et al.

Related authors

Carbon transfer, partitioning and residence time in the plant-soil system: a comparison of two 13CO2 labelling techniques
M. S. Studer, R. T. W. Siegwolf, and S. Abiven
Biogeosciences, 11, 1637–1648,,, 2014

Related subject area

Biogeochemistry: Stable Isotopes & Other Tracers
Carbon isotopic ratios of modern C3 and C4 vegetation on the Indian peninsula and changes along the plant–soil–river continuum – implications for vegetation reconstructions
Frédérique M. S. A. Kirkels, Hugo J. de Boer, Paulina Concha Hernández, Chris R. T. Martes, Marcel T. J. van der Meer, Sayak Basu, Muhammed O. Usman, and Francien Peterse
Biogeosciences, 19, 4107–4127,,, 2022
Short summary
Controls on nitrite oxidation in the upper Southern Ocean: insights from winter kinetics experiments in the Indian sector
Mhlangabezi Mdutyana, Tanya Marshall, Xin Sun, Jessica M. Burger, Sandy J. Thomalla, Bess B. Ward, and Sarah E. Fawcett
Biogeosciences, 19, 3425–3444,,, 2022
Short summary
Tracing the source of nitrate in a forested stream showing elevated concentrations during storm events
Weitian Ding, Urumu Tsunogai, Fumiko Nakagawa, Takashi Sambuichi, Hiroyuki Sase, Masayuki Morohashi, and Hiroki Yotsuyanagi
Biogeosciences, 19, 3247–3261,,, 2022
Short summary
Intra-skeletal variability in phosphate oxygen isotope composition reveals regional heterothermies in marine vertebrates
Nicolas Séon, Romain Amiot, Guillaume Suan, Christophe Lécuyer, François Fourel, Fabien Demaret, Arnauld Vinçon-Laugier, Sylvain Charbonnier, and Peggy Vincent
Biogeosciences, 19, 2671–2681,,, 2022
Short summary
Isotopic differences in soil–plant–atmosphere continuum composition and control factors of different vegetation zones on the northern slope of the Qilian Mountains
Yuwei Liu, Guofeng Zhu, Zhuanxia Zhang, Zhigang Sun, Leilei Yong, Liyuan Sang, Lei Wang, and Kailiang Zhao
Biogeosciences, 19, 877–889,,, 2022
Short summary

Cited articles

Augusti, A. and Schleucher, J.: The ins and outs of stable isotopes in plants, New Phytol., 174, 473–475, 2007.
Augusti, A., Betson, T. R., and Schleucher, J.: Hydrogen exchange during cellulose synthesis distinguishes climatic and biochemical isotope fractionations in tree rings, New Phytol., 172, 490–499, 2006.
Badeck, F.-W., Tcherkez, G., Nogués, S., Piel, C., and Ghashghaie, J.: Post-photosynthetic fractionation of stable carbon isotopes between plant organs – a widespread phenomenon, Rapid Commun. Mass Spectrom., 19, 1381–1391, 2005.
Barbour, M. M.: Stable oxygen isotope composition of plant tissue: a review, Funct. Plant Biol., 34, 83–94, 2007.
Bird, J. A. and Torn, M. S.: Fine roots vs. needles: a comparison of 13C and 15N dynamics in a ponderosa pine forest soil, Biogeochemistry, 79, 361–382, 2006.
Short summary
We present a new technique to label organic matter (OM) at its place of formation by the application of 13C, 18O and 2H through the gaseous phase. The label diffused into leaves was incorporated into assimilates and was detected in plant tissues. This technique can be applied in soil sciences, e.g. to trace the decomposition pathways of soil OM inputs, or in plant physiology and palaeoclimatic reconstruction, e.g. to further investigate the origin of the 18O and 2H signal in tree ring cellulose.
Final-revised paper