Articles | Volume 12, issue 11
Biogeosciences, 12, 3499–3512, 2015
Biogeosciences, 12, 3499–3512, 2015

Research article 09 Jun 2015

Research article | 09 Jun 2015

Disruption of metal ion homeostasis in soils is associated with nitrogen deposition-induced species loss in an Inner Mongolia steppe

Q.-Y. Tian1,*, N.-N. Liu1,2,*, W.-M. Bai1, L.-H. Li1, and W.-H. Zhang1,3 Q.-Y. Tian et al.
  • 1State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • 2University of Chinese Academy of Sciences, Beijing 100049, China
  • 3Research Network of Global Change Biology, Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China
  • *These authors contributed equally to this work.

Abstract. Enhanced deposition of atmospheric nitrogen (N) resulting from anthropogenic activities has negative impacts on plant diversity in ecosystems. Several mechanisms have been proposed to explain the species loss. Ion toxicity due to N deposition-induced soil acidification has been suggested to be responsible for species loss in acidic grasslands, while few studies have evaluated the role of soil-mediated homeostasis of ions in species loss under elevated N deposition in grasslands with neutral or alkaline soils. To determine whether soil-mediated processes are involved in changes in biodiversity induced by N deposition, the effects of 9-year N addition on soil properties, aboveground biomass (AGB) and species richness were investigated in an Inner Mongolia steppe. Low to moderate N addition rate (2, 4, 8 g N m−2 yr−1) significantly enhanced AGB of graminoids, while high N addition rate (≥ 16 g N m−2 yr−1) reduced AGB of forbs, leading to an overall increase in AGB of the community under low to moderate N addition rates. Forb richness was significantly reduced by N addition at rates greater than 8 g N m−2 yr−1, while no effect of N addition on graminoid richness was observed, resulting in decline in total species richness. N addition reduced soil pH, depleted base cations (Ca2+, Mg2+ and K+) and mobilized Mn2+, Fe3+, Cu2+ and Al3+ ions in soils. Soil inorganic-N concentration was negatively correlated with forb richness and biomass, explaining 23.59% variation of forb biomass. The concentrations of base cations (Ca2+ and Mg2+) and metal ions (Mn2+, Cu2+ and, Fe3+) showed positively and negatively linear correlation with forb richness, respectively. Changes in the metal ion concentrations accounted for 42.77% variation of forb richness, while reduction of base cations was not associated with the reduction in forb richness. These results reveal that patterns of plant biodiversity in the temperate steppe of Inner Mongolia are primarily driven by increases in metal ion availability, particularly enhanced release of soil Mn2+.

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Short summary
We demonstrate that N addition reduced species richness, acidified soil and disturbed nutrient homeostasis in soil in an Inner Mongolia steppe. We further reveal that an increase in inorganic-N concentration, depletion of base cations (Ca2+ and Mg2+) and mobilization of Mn2+ and Cu2+ in soils were involved in reduction in species richness in the temperate steppe of northern China, highlighting the involvement of nutrient mobilization in decline in species richness of alkaline grasslands.
Final-revised paper