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Volume 14, issue 10
Biogeosciences, 14, 2697–2714, 2017
https://doi.org/10.5194/bg-14-2697-2017
© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.
Biogeosciences, 14, 2697–2714, 2017
https://doi.org/10.5194/bg-14-2697-2017
© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.

Research article 31 May 2017

Research article | 31 May 2017

Functional diversity of microbial communities in pristine aquifers inferred by PLFA- and sequencing-based approaches

Valérie F. Schwab et al.

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AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
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AR: Author's response | RR: Referee report | ED: Editor decision
ED: Publish subject to minor revisions (Editor review) (13 Feb 2017) by Marcel van der Meer
AR by Valerie F. Schwab on behalf of the Authors (26 Feb 2017)  Author's response    Manuscript
ED: Publish subject to minor revisions (Editor review) (13 Mar 2017) by Marcel van der Meer
AR by Valerie F. Schwab on behalf of the Authors (23 Mar 2017)  Author's response    Manuscript
ED: Publish as is (04 Apr 2017) by Marcel van der Meer
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Short summary
We used phospholipid fatty acids (PLFAs) to link specific microbial markers to the spatio-temporal changes of groundwater physico-chemistry. PLFA-based functional groups were directly supported by DNA/RNA results. O2 resulted in increased eukaryotic biomass and abundance of nitrite-oxidizing bacteria but impeded anammox, sulphate-reducing and iron-reducing bacteria. Our study demonstrates the power of PLFA-based approaches to study the nature and activity of microorganisms in pristine aquifers.
We used phospholipid fatty acids (PLFAs) to link specific microbial markers to the...
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