Functional diversity of microbial communities in pristine aquifers inferred by PLFA- and sequencing-based approaches

Schwab, Valérie F.; Herrmann, Martina; Roth, Vanessa-Nina; Gleixner, Gerd; Lehmann, Robert; Pohnert, Georg; Trumbore, Susan; Küsel, Kirsten; Totsche, Kai U.

doi:https://doi.org/10.5194/bg-14-2697-2017

Articles | Volume 14, issue 10

https://doi.org/10.5194/bg-14-2697-2017

© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.

https://doi.org/10.5194/bg-14-2697-2017

© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.

Articles | Volume 14, issue 10

Research article

|

31 May 2017

Research article |

| 31 May 2017

Functional diversity of microbial communities in pristine aquifers inferred by PLFA- and sequencing-based approaches

Valérie F. Schwab, Martina Herrmann, Vanessa-Nina Roth, Gerd Gleixner, Robert Lehmann, Georg Pohnert, Susan Trumbore, Kirsten Küsel, and Kai U. Totsche

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Preprint (discussion started on 03 Nov 2016)
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Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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RC1: 'Review of Schwab et al. Functional diversity of microbial communities in pristine aquifers inferred by PLFA- and sequencing –based approaches', Anonymous Referee #1, 14 Dec 2016
- AC1: 'Answer anonymous Referee # 1', Valerie F. Schwab, 29 Jan 2017
RC2: 'Review of paper titled: Functional diversity of microbial communities in pristine aquifers inferred by PLFA and sequencing based approaches by Schwab, V et al.', Anonymous Referee #2, 12 Jan 2017
- AC2: 'Answer anonymous Referee # 2', Valerie F. Schwab, 29 Jan 2017

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

ED: Publish subject to minor revisions (Editor review) (13 Feb 2017) by Marcel van der Meer

AR by Valerie F. Schwab on behalf of the Authors (26 Feb 2017) Author's response Manuscript

ED: Publish subject to minor revisions (Editor review) (13 Mar 2017) by Marcel van der Meer

AR by Valerie F. Schwab on behalf of the Authors (23 Mar 2017) Author's response Manuscript

ED: Publish as is (04 Apr 2017) by Marcel van der Meer

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Short summary

We used phospholipid fatty acids (PLFAs) to link specific microbial markers to the spatio-temporal changes of groundwater physico-chemistry. PLFA-based functional groups were directly supported by DNA/RNA results. O₂ resulted in increased eukaryotic biomass and abundance of nitrite-oxidizing bacteria but impeded anammox, sulphate-reducing and iron-reducing bacteria. Our study demonstrates the power of PLFA-based approaches to study the nature and activity of microorganisms in pristine aquifers.