Articles | Volume 14, issue 10
Biogeosciences, 14, 2697–2714, 2017
https://doi.org/10.5194/bg-14-2697-2017
Biogeosciences, 14, 2697–2714, 2017
https://doi.org/10.5194/bg-14-2697-2017
Research article
31 May 2017
Research article | 31 May 2017

Functional diversity of microbial communities in pristine aquifers inferred by PLFA- and sequencing-based approaches

Valérie F. Schwab et al.

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Short summary
We used phospholipid fatty acids (PLFAs) to link specific microbial markers to the spatio-temporal changes of groundwater physico-chemistry. PLFA-based functional groups were directly supported by DNA/RNA results. O2 resulted in increased eukaryotic biomass and abundance of nitrite-oxidizing bacteria but impeded anammox, sulphate-reducing and iron-reducing bacteria. Our study demonstrates the power of PLFA-based approaches to study the nature and activity of microorganisms in pristine aquifers.
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