Biogeosciences
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Articles | Volume 14, issue 10
Articles | Volume 14, issue 10
https://doi.org/10.5194/bg-14-2697-2017
© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/bg-14-2697-2017
© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.
Articles | Volume 14, issue 10
Research article
 | 
31 May 2017
Research article |  | 31 May 2017

Functional diversity of microbial communities in pristine aquifers inferred by PLFA- and sequencing-based approaches

Valérie F. Schwab, Martina Herrmann, Vanessa-Nina Roth, Gerd Gleixner, Robert Lehmann, Georg Pohnert, Susan Trumbore, Kirsten Küsel, and Kai U. Totsche

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Short summary
We used phospholipid fatty acids (PLFAs) to link specific microbial markers to the spatio-temporal changes of groundwater physico-chemistry. PLFA-based functional groups were directly supported by DNA/RNA results. O2 resulted in increased eukaryotic biomass and abundance of nitrite-oxidizing bacteria but impeded anammox, sulphate-reducing and iron-reducing bacteria. Our study demonstrates the power of PLFA-based approaches to study the nature and activity of microorganisms in pristine aquifers.
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