Mechanisms of dissolved and labile particulate iron supply to shelf waters and phytoplankton blooms off South Georgia, Southern Ocean
- 1Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, SO14 3ZH Southampton, UK
- 2GEOMAR Helmholtz Centre for Ocean Research, Wischhofstr. 1–3, 24148 Kiel, Germany
- 3British Antarctic Survey, CB3 0ET Cambridge, UK
- 4School of Geography, Earth and Environmental Sciences, University of Plymouth, PL4 8AA Plymouth, UK
- 5Department of Earth Sciences, University of Oxford, OX1 3AN Oxford, UK
- 6Institut de Ciència i Tecnologia Ambientals & Departament de Física, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
- 7Plymouth Marine Laboratory, Prospect Place, The Hoe, PL1 3DH Plymouth, UK
Correspondence: Christian Schlosser (firstname.lastname@example.org)
The island of South Georgia is situated in the iron (Fe)-depleted Antarctic Circumpolar Current of the Southern Ocean. Iron emanating from its shelf system fuels large phytoplankton blooms downstream of the island, but the actual supply mechanisms are unclear. To address this, we present an inventory of Fe, manganese (Mn), and aluminium (Al) in shelf sediments, pore waters, and the water column in the vicinity of South Georgia, alongside data on zooplankton-mediated Fe cycling processes, and provide estimates of the relative dissolved Fe (DFe) fluxes from these sources. Seafloor sediments, modified by authigenic Fe precipitation, were the main particulate Fe source to shelf bottom waters as indicated by the similar Fe ∕ Mn and Fe ∕ Al ratios for shelf sediments and suspended particles in the water column. Less than 1 % of the total particulate Fe pool was leachable surface-adsorbed (labile) Fe and therefore potentially available to organisms. Pore waters formed the primary DFe source to shelf bottom waters, supplying 0.1–44 µmol DFe m−2 d−1. However, we estimate that only 0.41±0.26 µmol DFe m−2 d−1 was transferred to the surface mixed layer by vertical diffusive and advective mixing. Other trace metal sources to surface waters included glacial flour released by melting glaciers and via zooplankton egestion and excretion processes. On average 6.5±8.2 µmol m−2 d−1 of labile particulate Fe was supplied to the surface mixed layer via faecal pellets formed by Antarctic krill (Euphausia superba), with a further 1.1±2.2 µmol DFe m−2 d−1 released directly by the krill. The faecal pellets released by krill included seafloor-derived lithogenic and authigenic material and settled algal debris, in addition to freshly ingested suspended phytoplankton cells.
The Fe requirement of the phytoplankton blooms ∼ 1250 km downstream of South Georgia was estimated as 0.33±0.11 µmol m−2 d−1, with the DFe supply by horizontal/vertical mixing, deep winter mixing, and aeolian dust estimated as ∼0.12 µmol m−2 d−1. We hypothesize that a substantial contribution of DFe was provided through recycling of biogenically stored Fe following luxury Fe uptake by phytoplankton on the Fe-rich shelf. This process would allow Fe to be retained in the surface mixed layer of waters downstream of South Georgia through continuous recycling and biological uptake, supplying the large downstream phytoplankton blooms.
The Southern Ocean is the largest “high-nitrate, low-chlorophyll” (HNLC) region of the global ocean (Buesseler et al., 2004), as a consequence of low iron (Fe) supply and consequently reduced phytoplankton growth (Buesseler et al., 2004; Tsuda et al., 2009). Iron can be supplied to surface waters of the Southern Ocean by atmospheric dust inputs (Cassar et al., 2007; Gao et al., 2001), horizontal/vertical advective and diffusive mixing processes (de Jong et al., 2012), resuspension from shelf sediments (Kalnejais et al., 2010; Marsay et al., 2014), melting of icebergs and glaciers (Raiswell et al., 2008), and hydrothermal inputs (German et al., 2016). Despite the overall HNLC status of the Southern Ocean, regions in the wake of islands feature large seasonal phytoplankton blooms; the Fe sources to these blooms are however poorly constrained (de Jong et al., 2012; Planquette et al., 2007; Pollard et al., 2009).
Downstream of the island of South Georgia intense, long-lasting phytoplankton blooms have been observed which extend hundreds of kilometres and require an enhanced Fe supply. The blooms peak in austral summer (Borrione et al., 2014), stretch over an area of ∼750 000 km2 (Atkinson et al., 2001; Korb et al., 2004), and are responsible for the largest dissolved inorganic carbon deficit reported within the Antarctic Circumpolar Current (ACC) (Jones et al., 2012, 2015). As a consequence of the Fe fertilization, the waters in the vicinity of South Georgia support extensive phytoplankton blooms and a large biomass of zooplankton, fish, seabirds, and marine mammals, some of which are exploited commercially (Atkinson et al., 2001; Murphy et al., 2007).
South Georgia forms part of the volcanically active Scotia Arc in the Atlantic sector of the Southern Ocean and is surrounded by a broad 30–100 km wide shelf with an average (albeit highly variable) depth of ca. 200 m (Fig. 1). The island is situated between the Antarctic Polar Front (PF) and the Southern ACC Front (SACCF), within the general northeast flow of the ACC (Meredith et al., 2005; Whitehouse et al., 2008). The ACC surface waters are enriched in nitrate, phosphate, and silicic acid, but strongly depleted in most trace elements, notably Fe and manganese (Mn) (Browning et al., 2014). The large seasonal phytoplankton blooms downstream of South Georgia are thought to be supplied with Fe from the island during the passage of ACC waters (Borrione et al., 2014; Nielsdóttir et al., 2012).
In this study we provide the first comprehensive data set of dissolved and (labile) particulate Fe, Mn, and aluminium (Al) in sediments, pore waters, and the water column overlaying the shelf and shelf edge regions of South Georgia. We also include published data on the role of Antarctic krill in new Fe supply and recycling in this region (Schmidt et al., 2011, 2016). We discuss differences between the various analysed trace metal fractions and the supply routes of dissolved and (labile) particulate Fe, such as sedimentary pore water efflux, supply of sediment-derived particulate Fe to the surface mixed layer, efflux of Fe from glacial melting, and supply of Fe by faecal pellets of Antarctic krill. Furthermore, we discuss the productivity of the bloom region to the north of South Georgia in relation to the estimated Fe supply rates.
2.1 Cruises and sampling
Samples were collected during three research cruises to South Georgia in 2011 (JR247, JC055) and 2013 (JR274). While cruises JR247 and JR274 aimed to examine the pelagic shelf ecosystem by collection of predominantly water samples (and zooplankton during JR247) on the northern shelf, JC055 explored solely the composition of sediments on the South Georgia shelf. Cruise JR247 took place in January 2011 on RRS James Clark Ross, and 14 sites on the northern shelf and shelf edge of South Georgia were visited (stations 1–21; Fig. 1). Suspended particles were collected on acid-cleaned polycarbonate filters (1 µm pore size; Whatman) using in situ stand-alone pumping systems (SAPSs; Challenger Oceanic) attached to a Kevlar wire and deployed at 20, 50, and 150 m depth (Fig. 1, red dots). The filters were rinsed with deionized water (Milli-Q; Millipore), stored at −20∘C, and shipped frozen to the National Oceanography Centre Southampton (NOCS).
Subsurface seawater samples were collected by trace metal clean samplers (Ocean Test Equipment (OTE)) at 9 of the 14 SAPS locations (Fig. 1; black stars). Seawater samples were filtered using cartridge filter (0.2 µm Sartobran P 300, Sartorius) into acid-cleaned 125 mL low-density polyethylene (LDPE) bottles (Nalgene). Unfiltered samples were collected in 125 mL LDPE bottles for analysis of total dissolvable (TD) trace metals. Surface waters from the South Georgia shelf were collected using a tow fish deployed alongside the ship at 3–4 m depth. Samples were filtered in line using a cartridge filter (0.2 µm Sartobran P 300, Sartorius) and dispensed in acid-washed 125 mL LDPE bottles. Unfiltered surface seawater samples were also collected and dispensed in acid-washed 125 mL LDPE bottles. All seawater samples were acidified on board with ultra-clean HNO3 (15 M UpA grade, Romil) to pH 1.7 (22 µmol ). For a more detailed description of all sample-handling procedures, please see Sect. S1 in the Supplement.
In January and February 2013, RRS James Clark Ross cruise JR274 revisited South Georgia and collected surface seawater samples covering the shelf, shelf edge, and open-ocean areas around the island. Dissolved and TD trace elements in surface seawater samples were collected using the tow fish and treated similarly to samples from JR247. For a more detailed description of all sample-handling procedures, please see Sect. S1.
During the RRS James Cook cruise JC055 in February 2011, a megacorer (Bowers and Connelly type) was used to collect surface sediment and pore water samples on the southern side of South Georgia (there was no opportunity to sample the northern side of the island). Cores representing the intact sediment–water interface were retrieved from three sites on the southern shelf, at water depths of ca. 250 m (S1–S3) (Fig. 1, blue hexagons). Pore waters were separated by centrifugation under N2 atmosphere and filtered using cellulose nitrate syringe filters (0.2 µm pore size, Whatman; Homoky et al., 2012). Conjugate sediments were freeze-dried on board and stored at room temperature. A more detailed description of sediment and pore water sample-handling procedures is provided in Sect. S2.
Krill faecal pellets were obtained during on-board krill incubations performed during JR247. Incubations were performed in darkness in the laminar flow cabinet at ambient surface layer temperature. The krill were incubated in filtered seawater from the tow fish for up to ∼3 h immediately after capture, so that pellets obtained derived from material ingested in situ. These incubations and their results are described in more detail in Schmidt et al. (2016).
2.2 Trace metal analysis in suspended particles and krill faecal pellets
The labile trace metal fraction of suspended particles (SAPS) and krill faecal pellets was remobilized using a 25 % acetic acid solution (glacial SpA, Romil) following Planquette et al. (2011). The labile trace metal fraction is hereafter referred to as the leachable trace metal fraction (L). The remaining particles were digested on a hot plate, applying a mixture of aqua regia and hydrogen fluoride (Planquette et al., 2011). This fraction will be referred to as the refractory fraction (R). The particulate trace metal fraction (P) is the sum of leachable (L) and refractory (R) fractions. All samples were analysed by collision cell inductively coupled plasma–mass spectrometry (ICP-MS) (Thermo Fisher Scientific, XSeriesII). For a more detailed description of measured certified reference material see Sect. S1.
2.3 Trace metal analysis of seawater
The filtered and unfiltered seawater samples were stored for a period of 12 months prior to analysis. Concentrations of dissolved and total dissolvable Fe, Mn, and Al in seawater were determined by offline pre-concentration and isotope dilution/standard addition ICP-MS (Thermo Fisher Scientific Element2 XR) according to Rapp et al. (2017). For a more detailed description of the method and measured reference materials see Sect. S1.
2.4 Trace metal analysis of pore waters and sediments
Sub-samples of the bulk homogenized sediments were fully dissolved following an aqua regia and combined hydrofluoric–perchloric acid digestion method following Homoky et al. (2011). The acid digests and pore waters were analysed by ICP–optical emission spectrometry (OES) (Perkin Elmer Optima 4300DV). For a more detailed description of the method and measured reference materials see Sect. S2.
3.1 Supply routes of suspended particulate Fe, Mn, and Al
To avoid confusion, we will now define the terms biogenic, lithogenic, and authigenic particles, as they will be used frequently in the following sections. Biogenic particles refer to suspended organic particles, living and dead, such as phytoplankton cells. Lithogenic particles comprise mineral fragments, such as glacial flour and sediment particles. Authigenic particle include surface-scavenged trace metals and secondary minerals, such as amorphous FeO(OH) (e.g. goethite), that are formed in seawater and because of their age are insoluble to weak acid leaches (e.g. 25 % acetic acid solution).
3.1.1 Characterization of (the two) particulate trace metal fractions
Two different particulate fractions were obtained from samples collected during JR247: a particulate fraction from suspended particles collected using 1 µm pore size SAPS filters (P) and a leachable particulate fraction from unfiltered acidified seawater samples (LPUn) collected at the same depth. LPUn was calculated following Eq. (1):
Because of the different sampling approaches (SAPS vs. OTE water samplers), filter sizes (> 1 µm for SAPS vs. > 0.2 µm for dissolved seawater), and digestion procedures (aqua regia + HF for SAPS particles vs. water sample storage at pH 1.7 [22 µmol ]), concentrations of LPUn and P differed, but they showed similar distribution patterns in the water column (Figs. 2 and S1 in the Supplement, Tables 1 and 2). The concentrations of Fe, Mn, and Al in the LPUn fraction (LPUnFe, LPUnMn, LPUnAl) were usually slightly lower than the particulate fraction from suspended particles (PFe, PMn, PAl). The LPUn of unfiltered seawater samples corresponded to % of the PFe, 83±11 % of the PAl, and nearly 100±10 % of the PMn fractions obtained by SAPS. The average LPUn trace metal ratios (LPUnFe ∕ LPUnMn (1σ) and LPUnFe ∕ LPUnAl (n=69)) were about half of the elemental ratios of suspended particles obtained by SAPS (PFe ∕ PMn and PFe ∕ PAl 2 (n=42) (Fig. 3; Tables 1, 2 and 3)).
The lower concentrations of Fe and Al and the reduced elemental ratios in the LPUn compared to the P fractions suggest that an unknown fraction of particulate Fe and Al in seawater was not leached during the acidification procedure at pH 1.7 over 12 months. However, since P and LPUn displayed similar trends with depth (Figs. 2 and S1), LPUn was used in Sect. 3.1.3 and 3.3 as an indicator for the abundance of particulate trace metals at locations where particulate samples could not be retrieved by SAPS, e.g. in surface waters collected by the tow fish and depths greater than 150 m.
3.1.2 Suspended particulate trace metals in the water column
Concentrations of PFe, PMn, and PAl in the water column ranged from 0.87 to 267, 0.01 to 3.85, and 0.60 to 195 nmol L−1, respectively (Fig. 2, Table 2). Concentrations of LPUnFe, LPUnMn, and LPUnAl ranged from 1 to 118, 0.01 to 100, and 1 to 141 nmol L−1, respectively (Fig. 2, Table 1). Below the isopycnal density layer 27.05 kg m−3, located at ∼50–70 m depth, P and LPUn increased with depth and showed a maximum near the seafloor of e.g. 207 nmol L−1 for PFe and 112 nmol L−1 for LPUnFe (#17, Table 2). Most sites on the shelf (bottom depth ≤ 260 m; #9/10, #13, #14, #17, and #21) showed seafloor maxima, in agreement with other shelf studies. For example, Milne et al. (2017) reported concentrations of up to 140 nmol L−1 for PFe and 800 nmol L−1 for PAl in bottom waters on the west African shelf, and Chase et al. (2005) showed bottom water maxima of up to 400 nmol L−1 for LPUnFe off the Oregon coast.
Strong linear relationships between elements were observed for suspended particles (SAPS) obtained from above and below the 27.05 kg m−3 isopycnal, with elemental ratios of PFe ∕ PMn , PFe ∕ PAl , and PMn ∕ PAl (n=42) (Fig. 3, Tables 2 and 3). These elemental ratios were higher than those reported for the earth's crust (Fe ∕ Mn Fe ∕ Al =0.2, Mn ∕ Al =0.0035; Wedepohl, 1995) and sediment samples collected to the south of the island (mean sediment surface layer of S1, S2, S3; SFe ∕ SMn , SFe ∕ SAl , SMn ∕ SAl (Fig. 4, Tables 3 and 4)), suggesting that the suspended particles were more enriched in Fe than crustal and lithogenic particles (Table 3).
The Fe ∕ Mn ratios among different phytoplankton species show strong variations but are typically much lower (Fe ∕ Mn ∼1.7; Ho et al., 2003), with also lower Fe concentrations than lithogenic (sediment) particles (cellular Fe concentration of phytoplankton ∼0.7 mmol kg−1, Ho et al., 2003; upper crust ∼550 mmol kg−1, Wedepohl, 1995)). A prevalence of biogenic particles in the suspended particle pool would be expected to result in reduced PFe ∕ PMn ratios in our SAPS samples to values less than 51.5, as observed in the sediments.
It is most likely that authigenic Fe precipitation (e.g. dissolved Fe (DFe) was scavenged onto sediment particles) increased the Fe-to-Al (and Fe-to-Mn) ratio of suspended particles (PFe ∕ PAl =1.25; PFe ∕ PMn =68.0) compared to sediment particles (SFe ∕ SAl =0.34; SFe ∕ SMn =51.5). At seawater pH (∼ pH 8), dissolved Fe(III) is rapidly hydrolysed to soluble Fe(III)(OH)3 (< 0.02 µm), which readily accumulates as nanometre-sized colloids (0.02–0.2 µm) (Liu and Millero, 2002) and particles (> 0.2 µm) (own observation). It has also been shown that both soluble and colloidal Fe are attracted by charged surfaces (organic and inorganic particle surfaces), a process that removes DFe and simultaneously increases the amount of particulate Fe in seawater over time (Schlosser et al., 2011).
A range of mechanisms deliver suspended particles to the surface waters. These transport mechanisms will be discussed in the following section.
3.1.3 Glacial outflow and zooplankton feeding activity
While most stations on the shelf showed bottom water maxima of particulate metals, at three sampling sites located on the shelf (#18) and shelf edge (#15/16 and #19/20) the particulate trace metal concentrations featured maxima in the top 100 m of the water column (Figs. 2 and 5). At site #19/20, ca. 100 km away from the coast with a water depth of 1741 m, the PFe concentration at 20 m depth was 97 nmol L−1, similar to LPUnFe (Fig. 5). The elemental ratio PFe ∕ PAl of these samples (e.g. 1.01 for site #19/20, 20 m depth) was close to the average ratio (PFe ∕ PAl =1.25), indicating that lithogenic and authigenic Fe dominated the suspended particulate pool in these surface waters.
The surface water maxima of trace metals could have two supply routes: (1) lateral transport of waters containing lithogenic and authigenic particles from shallow island shelf sediments, and (2) transport of glacial particles following melt processes. The reduced salinities (∼33.3 PSU) recorded in surface waters in Cumberland Bay and ∼50 km offshore of South Georgia (∼33.8) (Figs. 6c and S2) provide an indication of glacial outflow, melting of icebergs, and run-off of meltwater streams. Enhanced LPUnFe concentrations of 2.2 µmol L−1 in low-salinity surface waters inside Cumberland Bay are indicative of a meltwater source (LPUn concentration used as only water samples from the tow fish available). The LPUnFe concentration decreased strongly with increasing distance from the coast and exhibited an abrupt reduction to 1–5 nmol Fe L−1 at the shelf edge ∼100 km offshore. A similar distribution pattern was observed for LPUnMn (Fig. 6d) and LPUnAl (not shown), for cruises JR247 and JR274. Glacial melt has been reported as an important source of particulate material in the vicinity of the Antarctic Peninsula (de Jong et al., 2012). For example, Gerringa et al. (2012) documented elevated total dissolvable Fe concentration of up to 106 nmol L−1 near the Pine Island Glacier in the Amundsen Sea, and Raiswell et al. (2008) estimated that per year 1.6 Gmol of nanoparticulate Fe, associated with lithogenic particles, is delivered to the Southern Ocean by melting ice.
Locally elevated particulate metal concentrations in surface waters may also be related to production of faecal pellets by swarms of Antarctic krill (Schmidt et al., 2016). High abundances of Antarctic krill were estimated from acoustic backscattering observations (Fielding et al., 2014), and large numbers of faecal pellets were observed on the SAPS filters during cruise JR247. The stomach content of Antarctic krill contained up to 90 % sediment particles by volume, an observation that was attributed to filter feeding by these organisms on phytoplankton and seabed detritus, with incidental ingestion of deep-ocean sediments (Schmidt et al., 2011) and glacial flour (Schmidt et al., 2016). Krill thus take up lithogenic (sediment) particles and transfer these into the surface ocean through the egestion of faecal pellets (Schmidt et al., 2016). The trace metal contents of krill faecal pellets collected during on-board incubation experiments during JR247 ranged from 0.88 to 67.14 µg Fe mg−1 dry weight (n=27) (Table 5) (Schmidt et al. 2016). The molar ratios of the faecal pellets (PFe ∕ PAl and PMn ∕ PAl ) were similar to those for sediment particles (SFe ∕ SAl and SMn ∕ SAl ; Tables 1, 2, 3, and 4), indicating that Fe in krill faecal pellets was predominately associated with lithogenic (sediments) and/or glacial flour particles, as also reported by Schmidt et al. (2016). In contrast, the molar ratio of faecal pellets (PFe ∕ PMn ) was higher than that of sediments (SFe ∕ SMn ) but just slightly higher than that of suspended SAPS particles (PFe ∕ PAl ). The observed variability in the PFe ∕ PAl and PFe ∕ PMn ratio in the various particle pools is therefore a consequence of different amounts of lithogenic and authigenic particles.
3.2 Supply routes of dissolved Fe, Mn, and Al
Concentrations of DFe, DMn, and DAl in the water column showed strong variations and ranged from ∼0.1 to 25.9, 0.3 to 19.6, and 0.1 to 18.4 nmol L−1, respectively (Figs. 2, 5 and 7), with highest values in the surface waters in Cumberland Bay and lowest beyond the shelf break (Fig. 6). Dissolved Fe concentrations from this study are in agreement with reported DFe near the Antarctic Peninsula (0.6–14.6 nmol L−1; de Jong et al., 2012) and Crozet Islands (0.1–2.5 nmol L−1; Planquette et al., 2007). Sources and sinks of dissolved trace metals, and their distribution in the water column are discussed in the following sections.
3.2.1 Supply from sediment pore waters
Elevated pore water concentrations of Fe and Mn (FePW and MnPW) were observed in sediments from the southern shelf sites at water depths of around 250 m and ranged from 0.5 to 110 µmol L−1 for Fe and 0.1 to 2 µmol L−1 for Mn (Fig. 7 and Table S2 in the Supplement). The down-core distributions of FePW and MnPW were consistent with microbial dissimilatory Mn and Fe reduction during organic matter oxidation (Canfield and Thamdrup, 2009), and thus concentrations were elevated at defined depth horizons controlled by their redox potential (Eh) (Bonneville et al., 2009; Raiswell and Canfield, 2012). The FePW and MnPW concentrations near the sediment–seawater interface were used to calculate fluxes of Fe and Mn to bottom waters following diffusion of reduced Fe and Mn species across an oxygenated layer in surface sediments. These calculations were performed following Boudreau and Scott (1978) and Homoky et al. (2012), and are described in detailed in the Sect. S3 and Table S1. We are aware that our calculated fluxes represent minimum estimates of pore water efflux, which under natural conditions is supplemented by advection due to bioirrigation, bioturbation, and bottom water currents (Homoky et al., 2016). In addition, sediment cores were collected on the southern shelf, while seawater and particulate samples were collected on the northern shelf side. The benthic Fe fluxes for the southern shelf may be lower than those on the northern shelf, as an elevated primary productivity and enhanced particle export on the northern side will result in enhanced bacterial respiration, which reduces Eh and promotes the dissolution of Fe oxides with subsequent release of Fe into bottom waters.
Notwithstanding the above issues, we calculated substantial benthic fluxes from sediment pore waters to bottom waters on the southern shelf for FePW of < 0.1 to 44.4 µmol m−2 d−1 and MnPW of 0.6 to 4.1 µmol m−2 d−1. The upper flux values for Fe are comparable to those reported for dysoxic and river-dominated continental margins (3.5–55 µmol m−2 d−1; Homoky et al., 2012), seasonal maxima of temperate and oxic shelf seas (23–31 µmol m−2 d−1; Klar et al., 2017), and shelf sediments off the Antarctic Peninsula (1.3–15.5 µmol m−2 d−1; de Jong et al., 2012). The Mn fluxes were relatively low for shelf environments, with for example fluxes of 70–4450 µmol m−2 d−1 reported for Baltic and Black Sea sediments (Pakhomova et al., 2007). The Fe pore water fluxes from the South Georgia shelf sediments, which extend over an area of ca. 40 000 km2, indicate that these may serve as an important year-round source to overlying waters, totalling 4 to 1728 kmol DFe d−1 and 25 to 164 kmol DMn d−1.
Benthic release of trace-metal-enriched pore waters shaped the distributions of dissolved trace metals in bottom waters on the shelf. Concentrations of DFe, DMn, and DAl were enhanced at isopycnals > 27.05 kg m−3 (e.g. DFe up to 7.70 nmol L−1 at site #21, Table 1) compared to surface waters (e.g. DFe as low as 0.30 nmol L−1 at site #13, Table 1; Figs. 2 and 7). Trace-metal-enriched bottom waters were also observed at sites #13, #14, #17, and #18 (Fig. 2). The molar DFe ∕ DMn ratios in oxygenated bottom waters varied between 1.1 and 3.5 and were thus similar to pore waters near the sediment–seawater interface (0–1 cm depth; FePW/Mn; Fig. 7). The similar trace metal ratios suggest that Fe and Mn in enriched pore waters crossed the sediment–bottom water interface and accumulated in shelf bottom waters.
To determine the vertical DFe fluxes from near bottom to surface waters, we employed a method outlined by de Jong et al. (2012) and calculated both the advective and diffusive flux terms, which are not affected by the benthic Fe and Mn fluxes. We included the advective term in our calculations, because it has been shown that internal waves that cross shallow topographies and wind shear stress produce strong turbulence that facilitate Eckman upwelling (vertical advection) on the shelf (Kurapov et al., 2010; Moore, 2000; Wolanski and Delesalle, 1995). Applying literature values from the Southern Ocean for vertical diffusivity ( m2 s−1; Charette et al., 2007) and advective velocity ( m s−1; de Jong et al., 2012), an average vertical DFe flux on the shelf of 0.41±0.26 µmol m−2 d−1 from subsurface waters into the surface mixed layer was estimated (Sect. S4). The surface mixed-layer depth was determined by a density criterion (∼0.03 kg m−3; de Boyer Montégut et al., 2004) and was located at ∼50 m depth. About 38 % of the DFe flux was related to Ekman upwelling (advective term) and 62 % to the diffusive flux. This vertical flux is at the lower end of the calculated benthic flux from this study (FePW < 0.1 to 44.4 µmol m−2 d−1) and agrees with values reported for other Southern Ocean shelf regions near the Antarctic Peninsula (within 20–70 km from the coast: µmol m−2 d−1; de Jong et al., 2012) and the Crozet Islands (only diffusive flux of 0.06 µmol m−2 d−1; Planquette et al., 2007).
3.2.2 DFe supply from the leachable fraction of particles
The analytical protocol for the analysis of SAPS-collected particulate material allows separate estimation of the refractory and leachable fractions of trace elements (R and L, respectively). The R fraction of the suspended matter is considered to include silicates and aged oxide minerals, while the L fraction represents predominantly fresh oxyhydroxides, biogenic material, and loosely bound surface-associated elements which are readily remobilized using leaching procedures (Berger et al., 2008).
Concentrations of LFe, LMn, and LAl in the water column showed strong variations, ranging from a few picomoles to several nanomoles per litre (Table 2). On average, LFe and LAl concentrations at 150 m depth (∼ 1.3 nmol LFe L−1 and ∼0.95 nmol LAl L−1) were significantly higher than at 20 and 50 m: LFe =0.3 nmol L−1 (Student t test: ) and LAl =0.43 nmol L−1 (Student t test: ). The LMn concentrations did not vary strongly and remained near constant throughout the top 150 m (LMn =8.9 pmol L−1; Student t test: ). The average contribution of L to the particulate pool P (R+L) was low: 0.83±1.13 % for Fe, 2.55±1.58 % for Mn, and 2.42±1.32 % for Al (Table 2). A study conducted in the North Pacific near the Columbia River outflow reported considerably higher L fractions (e.g. 6.6±3.0 % of Fe, 78.7±14.0 % of Mn, 6.3±2.0 % of Al; Berger et al., 2008), which was attributed to enhanced biogenic particle levels in the low-salinity waters of the river (Berger et al., 2008). In contrast, results from our study showed that particulate trace metals predominately had a refractory component (R), indicating that Fe, Mn, and Al were mainly incorporated in mineral structures unaffected by a weak acid leach (e.g. aged oxyhydroxides and alumosilicates).
A weak linear relationship between R and L was observed for Fe (R2=0.57, n=41), Mn (R2=0.64, n=41), and Al (R2=0.63, n=41) (Figs. S3 and S4), indicating that the L fraction included mainly lithogenic and authigenic (e.g. scavenged) Fe, Mn, and Al and that not much LFe was incorporated in biogenic particles. The scavenging of dissolved trace metals by charged particle surfaces is established (Homoky et al., 2012; Koschinsky et al., 2003), but how well Fe and other trace metals can be remobilized from marine particle surfaces and which process may modify their availability over time are not yet well constrained (Achterberg et al., 2018; Fitzsimmons and Boyle, 2014; Milne et al., 2017).
For instance, scavenged Fe is reported to exchange with DFe in the water column of the tropical and high-latitude North Atlantic (Achterberg et al., 2018; Fitzsimmons and Boyle, 2014; Milne et al., 2017). In addition, recent work has concluded that zooplankton grazing and the production of faecal pellets remobilize DFe from lithogenic and biogenic particles (Giering et al., 2012; Schmidt et al., 2016). In contrast, freshly produced inorganic Fe(III) oxyhydroxide (FeOOH × nH2O) precipitates in seawater are accessible but are subject to chemical and structural conversions that lead to less leachable Fe with time (Yoshida et al., 2006).
3.2.3 DFe supply from Antarctic krill
Elevated dissolved trace metal concentrations in the top 200 m of the water column coincided with elevated particulate trace metal concentrations at sites #11/12, #15/16, #18, and #19/20 (Figs. 2, 5, and 7). The SAPS filters from these stations contained a high load of krill faecal pellets. To elucidate the relationship between dissolved trace metal concentrations and the local abundance of Antarctic krill and their faecal pellets, krill were caught and incubated on board the vessel as described in Schmidt et al. (2016).
Antarctic krill excretion rates of DFe were variable, relating positively to the extent of recent ingestion of diatoms. However, on average krill released nmol DFe individual−1 d−1 (Schmidt et al., 2016). When a mean krill abundance of 465±588 individuals m−2, estimated from acoustic backscattering measurements (Fielding et al., 2014), was applied, krill excreted 1.1±2.2 µmol DFe m−2 d−1 into the top 300 m of the water column (Schmidt et al., 2016). In addition, krill produced ca. 1.8±1.6 mg of faecal pellets per individual per day, containing ca. 0.30±0.33 µmol Fe mg−1. Particle leaches performed on those faecal pellet samples with 25 % acetic acid showed that on average 2.5±2.1 % (9.3±13.3 nmol Fe mg−1) of the total Fe in these pellets could be remobilized (Table 5), which would equate to a production of 14±24 nmol LFe individual−1 d−1. By multiplying the mean LFe by the ambient krill density used above, we calculate a LFe flux of 6.5±8.2 µmol m−2 d−1 from the faecal pellets to the water column.
Since krill are mobile animals, questions remain over where the major part of the LFe flux occurs and what the fate of this Fe source is. The highest krill abundances have generally (but not exclusively) been recorded in the top 100 m layer (Fielding et al., 2014), and hence a large proportion of this LFe flux from krill is likely to occur in the upper waters. Notwithstanding our current uncertainties over the depths of origin and fate, the LFe flux from krill faecal pellets and the release of DFe were on average an order of magnitude higher than estimated vertical diffusive and advective DFe fluxes, with other grazers, such as copepods and salps, adding to the recycled-flux estimates. This illustrates the importance of zooplankton-mediated Fe cycling, in agreement with previous studies (Hutchins and Bruland, 1994; Sato et al., 2007).
The experimental set-up did not allow us to establish the origin of the Fe released by krill, being both “recycled” Fe from biogenic material and “new” Fe from lithogenic (and authigenic) material. However, Schmidt et al. (2016) concluded that zooplankton gut passage mobilizes lithogenic Fe, and they showed that there are strong spatial patterns in the organic and lithogenic make-up of faecal pellets. This included an exponential decline in the quantity of lithogenic particles in krill stomachs with distance from sources of glacial flour on the northern South Georgia coast. For instance, the lithogenic content at one site on the shelf contributed ∼90 % of stomach content volume, suggesting that a large quantity of the accessible Fe was remobilized from those inorganic particles.
3.3 Offshore transport of trace-metal-enriched water masses
Along a W–E transect (Fig. 1; #14 via # 13 to #11/12), lateral water mass transport carried suspended particles offshore. Because of the small size of the SAPS particulate data set (two data points), we considered the LPUn fraction for this transect (Fig. 1). Indeed, elevated concentrations of the P and LPUn metal fractions were observed in subsurface waters that had been in recent contact with the shelf. These metal-enriched waters, detected at the eastern shelf edge site #11/12 between 200 and 400 m water depth (Figs. 1 and 4), exhibited similar temperature and salinity signatures to shelf bottom waters. Furthermore, the elemental ratios of the LPUn fraction in these waters were similar to the particles in the surface sediments (S1, S2, and S3) and the resuspended particles in the bottom boundary layer (#13 and #14) on the shallow shelf (Fig. 4). A similar distribution was also found for the P fractions, but limited to sites #13 and #14, as SAPS were not deployed below 150 m at the shelf edge site #11/12.
The LPUnFe concentration decreased offshore with distance from the island: from site #14 at 200 m depth (LPUnFe =82.26 nmol L−1; water depth =255 m) to site #13 at 100 m depth (LPUnFe =34.06 nmol L−1; water depth =130 m) to site #11/12 between 200 and 400 m depth (LPUnFe ∼10.18 nmol L−1; water depth =750 m) (Fig. 4 and Table 1). A similar decrease was observed for the SAPS Fe data: from site #14 at 150 m depth (PFe =31.12 nmol L−1) to site #13 at 100 m depth (PFe =10.23 nmol L−1). The decrease of PFe and LPUnFe with increasing distance to the coast is in agreement with previous observations for the western subarctic Pacific (Lam and Bishop, 2008), which reported elevated LFe concentrations in the range of 0.6 to 3.8 nmol L−1 in subsurface waters between 100 and 200 m depth along the Kamchatka shelf and related this observation to offshore water mass transport. However, we assume that particles in the deep particulate Fe maximum are not transported over very large distances, due to their tendency to sink, and thus do not significantly contribute to the offshore Fe supply (Sect. 3.4).
Consistent with the observed P and LPUn distributions, elevated dissolved metal concentrations at depths between 200 and 400 m at site #11/12 indicated that trace-metal-enriched shelf bottom waters were transported offshore (Fig. 7). However, dissolved trace metal concentrations were more variable than P and LPUn, and in the case of DMn they were highest at depths at shelf edge site #11/12. Notwithstanding the above issue, for horizontal flux calculations we used the entire DFe data set for water depths between 100 and 400 m. Average DFe concentrations in this depth range were highly variable and did not follow an exponential or power law function with distance from the coast (Fig. S5), which is necessary to determine scale length and horizontal diffusivity (Kh) (de Jong et al., 2012). As a result, horizontal flux calculations from the data could not be executed.
The distribution of dissolved trace metals in surface waters indicated that there was a limited transfer of DFe beyond the shelf break into the bloom region. Surface samples showed that DFe concentrations were strongly enriched in surface waters on the shelf (0.3–25.9 nmol L−1, Fig. 6b), while DFe concentrations beyond the shelf break decreased abruptly to concentrations < 0.2 nmol L−1 (Fig. 6b). This indicates that DFe was quickly removed from ACC surface waters following passage of the island. However, previous studies in the region suggest DFe transfer beyond the shelf break of South Georgia (Borrione et al., 2014; Nielsdóttir et al., 2012). Nielsdóttir et al. (2012) reported surface waters downstream of the island shelf with up to 2 nmol DFe L−1, with seasonal variations and highest concentrations during austral summer in January/February 2008. Dissolved Fe data from JR247 (2011) and JR274 (2012) were also obtained during the summer season but indicated rapid reduction in concentrations through mixing with DFe-depleted ACC water, biological uptake, and/or particle scavenging (authigenic precipitation).
3.4 Iron budget in the bloom region
Large seasonal phytoplankton blooms downstream of South Georgia recorded by Earth-observing satellites are initiated by Fe supplied by the South Georgia island–shelf system during the passage of ACC waters (Fig. 1) (Borrione et al., 2014; Nielsdóttir et al., 2012). Based on our study, the main DFe sources during this passage of the ACC were benthic release and vertical mixing, release of DFe from krill and krill faecal pellets, and supply of particles from run-off and glacial meltwater. In the following sections we will discuss the strength of each DFe source in the bloom region ca. 1250 km downstream of the island and estimate how much DFe is required to stimulate the elevated primary productivity in that region. Because of the lack of observational data for the region, this part of the study combines literature values from different Southern Ocean studies. This approach contains large uncertainties, which are discussed in detail in Sect. 3.4.6.
3.4.1 Phytoplankton Fe requirements in the phytoplankton bloom region
The surface ocean in the vicinity of South Georgia during the austral summer features strongly elevated biomass production (Gilpin et al., 2002) and represents the largest known CO2 sink in the ACC (12.9 mmol C m−2 d−1; Jones et al., 2012). The Fe requirements of the phytoplankton community in austral summer within the bloom that reaches several hundred kilometres downstream of the island were determined by combining satellite-depth-integrated net primary production data derived from a phytoplankton pigment adsorption (αph)-based model (62±21 mmol C m−2 d−1; Ma et al., 2014) over the period of 2003–2010 with an average intracellular Fe ∕ C ratio obtained from five Southern Ocean diatom species (5.23±2.84 µmol Fe mol−1 C; Strzepek et al., 2011). This approach yielded an approximate Fe requirement of 0.33±0.11 µmol DFe m−2 d−1 for the phytoplankton community (Fig. 8). For a more detailed description of the applied values and calculations see Sect. S4.
3.4.2 Horizontal and vertical mixing
De Jong et al. (2012) reported that horizontal and vertical advective, diffusive (diapycnal), and deep winter mixing downstream (1250–1570 km) of the Antarctic Peninsula (between 51 and 59∘ S) supplied DFe to the surface waters in quantities that exceeded the DFe requirement of primary producers during austral summer (0.13±0.04 µmol DFe m−2 d−1). In their study region, de Jong et al. (2012) determined that µmol DFe m−2 d−1 was supplied by horizontal and vertical fluxes, of which 91 % of the vertical flux was attributed to Ekman upwelling (advective term), and 43 % of the entire DFe flux was supplied by deep winter mixing. Tagliabue et al. (2014) reported similar model estimates for the region that is located south of the PF and characterized by strong Ekman upwelling and winter entrainment.
For the bloom region downstream of South Georgia, model calculations by Tagliabue et al. (2014) indicated that less than 0.0003 µmol DFe m−2 d−1 was supplied by diapycnal mixing and that µmol DFe m−2 d−1 was removed by Ekman downwelling. For the vertical flux component, this yields an overall loss of DFe of µmol DFe m−2 d−1 ()) in the bloom region north of South Georgia (Fig. 8).
Because the sampling in our study was not suitable for calculations of the horizontal flux, we applied the horizontal flux estimates from de Jong et al. (2012) for our own Fe budget. For a region ca. 1250 km downstream of a source, calculations according to de Jong et al. (2012) suggest that ca. 0.11±0.03 µmol DFe m−2 d−1 is supplied to the bloom region by horizontal advection and diffusion (Fig. 8).
3.4.3 Deep winter mixing
The entrainment of new DFe during winter represents an important Fe source to surface waters in the Southern Ocean (de Jong et al., 2012; Tagliabue et al., 2014). Elevated DFe concentrations in subsurface waters support primary production in the austral spring following entrainment by deep winter mixing. Model estimates showed that DFe supplied by winter mixing, together with diapycnal mixing, matches the Fe requirements at most low-productivity sites in the Southern Ocean. However, deep winter mixing at the highly productive sites north of South Georgia supplies only ∼ 0.011 µmol m−2 d−1 (Tagliabue et al., 2014) (Fig. 8). Later in the season primary productivity in surface waters is considered to rely strongly on Fe derived from recycling of biogenic material (Boyd et al., 2015).
3.4.4 Dust deposition
Dissolved Fe supplied by the deposition of aeolian dust is considered to be an important source to the Southern Ocean (Conway et al., 2015; Gabric et al., 2010; Gassó and Stein, 2007). Aeolian flux model estimates, supplied by Borrione et al. (2014) using a regional South Georgia model, suggested that up to 8 µmol Fe m−2 d−1 is delivered to the bloom regions downstream of South Georgia by dry and wet deposition. However, reliable dry- and wet-deposition estimates for the Southern Ocean are limited. Data from the South Atlantic along 40∘ S, ∼1000 km north of South Georgia, showed that rather low levels of DFe (∼0.002 µmol m−2 d−1) are supplied by dry deposition (Chance et al., 2015). In addition, µmol DFe m−2 d−1 are delivered sporadically to the 40∘ S area by wet deposition (Chance et al., 2015). However, even when assuming that similar wet-deposition fluxes occur north of South Georgia, fertilization with DFe is temporally and spatially limited. Furthermore, it is very unlikely that such sporadic events could cause long-lasting and far-extending phytoplankton blooms strictly constrained between the PF and the SACCF.
3.4.5 Luxury Fe uptake on the shelf
Our conservative estimate of DFe supply to the bloom region by vertical/horizontal mixing, deep winter entrainment, and dust deposition (< 0.12 µmol Fe m−2 d−1) covers only ∼30 % of the estimated phytoplankton requirements (∼ 0.33 µmol Fe m−2 d−1) (Fig. 8). We hypothesize that the missing supply of ∼0.21 µmol DFe m−2 d−1 is supplied to the bloom region through the offshore advection of phytoplankton cells that are enriched in labile Fe. It has been demonstrated that Fe-rich biogenic particles can be created by luxury iron uptake of diatoms (Iwade et al., 2006; Marchetti et al., 2009). Using bottle incubation experiments, Iwade et al. (2006) showed that under Fe-replete conditions the coastal diatom Chaetoceros sociale stores more intracellular Fe than needed for the production of essential enzymes and proteins. We therefore hypothesize that phytoplankton cells that grew under excess nutrient supply on the South Georgia shelf stored more Fe than needed for their metabolic processes. Due to subsequent cycles of grazing, lysis or bacterial decomposition, this iron can be remobilized in surface waters and made available for renewed phytoplankton uptake.
High Fe recycling efficiencies, described by the fe ratio (Boyd et al., 2005), are required to maintain the cycle of remineralization and uptake in the euphotic zone. This counteracts the loss of particulate Fe by vertical export. Boyd et al. (2015) reported the highest recycling efficiencies of ∼90 % for subantarctic DFe-depleted waters such as downstream of South Georgia. Further, these workers showed that the degree of recycling is controlled by the abundance of prokaryotes with a high Fe quota, such as cyanobacteria, and particularly by grazing zooplankton. The waters off South Georgia feature among the highest biomasses worldwide of metazoan grazers (Atkinson et al., 2001). These large grazers, chiefly copepods and Antarctic krill, are able to efficiently ingest large diatoms, including species that are known to store luxury iron (Atkinson, 1994; Hamm et al., 2003), thereby disintegrating cell membranes and releasing trace metals.
In recent years it has become apparent that the recycling of biogenic particles in the euphotic zone is a critical mechanism that maintains primary production, especially when the dissolved nutrient pools become exhausted (Boyd et al., 2015; Tagliabue et al., 2014). However, uncertainties remain over the degree to which Fe is lost during each cycle of uptake and remineralization. Thus more research is needed, especially fieldwork that encompasses the community structures (bacteria, phytoplankton, zooplankton, and higher predators; Ratnarajah et al., 2017; Wing et al., 2014), the degree of recycling for macro- and micro-nutrients in the euphotic zone, and loss of Fe through vertical export.
An alternative explanation to our suggestion that recycling of luxury-iron-enriched biota contributes to the downstream bloom is that iron is adsorbed directly onto particles that are advected directly offshore. For example freshly precipitated Fe(III) oxyhydroxides (FeOOH × nH2O) may be adsorbed onto biogenic and non-biogenic material. Iron freshly absorbed onto biogenic and non-biogenic material can be released and incorporated by phytoplankton and bacteria. However, the bioavailability of adsorbed and inorganic Fe changes over time. Both Wells et al. (1991) and Chen and Wang (2001) demonstrated that the bioavailability of freshly precipitated FeOOH and Fe adsorbed onto colloids/inorganic particles decreases over time. This is primarily due to the dehydration of the loosely packed structure that is subsequently transferred into amorphous FeOOH in the mineral structure goethite. Because of this we suggest that the majority of Fe from inorganic FeOOH or Fe adsorbed onto particles must be released and utilized in an early stage of the voyage, mainly on the shelf or shortly after the shelf break.
3.4.6 Budget uncertainties
Estimates for Fe budgets are challenging and often contain large uncertainties. This is primarily due to the lack of site- and time-specific flux data. Moreover, the mean annual estimates necessary for reliable supply calculations reach a high level of accuracy only after the same region has been monitored multiple times to cover seasonal and annual anomalies. In the following, we will discuss the uncertainty of the different Fe fluxes in the blooming region north of South Georgia.
We identified three main processes that together account for ∼98 % of the total Fe flux in the blooming region and thus contribute largest uncertainties: the horizontal flux, dry/wet deposition, and winter entrainment. Horizontal flux estimates of this study rely on literature values that were collected offshore of the Antarctic Peninsula. In contrast, South Georgia is an island with a confined shelf region, and thus horizontal DFe fluxes may differ greatly. Furthermore, we showed not only that dry-deposition dust fluxes are generally low but also that the Fe flux can be supplemented strongly by sporadic wet-deposition events ( µmol DFe m−2 d−1) (Chance et al., 2015). Atmospheric fluxes are variable, illustrated by the large standard deviation of the wet-deposition Fe fluxes obtained at 40∘ S. Furthermore, to determine the magnitude of the seasonal DFe winter entrainment reliable estimates of the winter mixing layer depth (WMLD) and the ferrocline are required. Even though the WMLD can be estimated very precisely using Argo float data, the depth of ferrocline in Tagliabue et al. (2014) is based on 140 unique observations distributed over the entire Southern Ocean. Due to this, regional anomalies are not captured. In addition to the DFe fluxes in the blooming region, we also assume that the biological Fe demand estimated for the phytoplankton community contributes a large error. The biological Fe requirements were determined using satellite-derived net primary production data and an average intracellular Fe ∕ C ratio derived from five different diatom species native to the Southern Ocean. Both parameters are not well constrained, and because of the lack of observational data we applied the lowest intracellular Fe ∕ C ratio available in the literature (Strzepek et al., 2011). However, we found that even small changes of both parameters change the estimated Fe availability in the bloom region strongly. Nevertheless, flux estimates even with large uncertainties can help us understand the degree of the nutrient supply vs. consumption by organisms and help to pinpoint the limitation of the estimates made. To ultimately reduce the level of uncertainty and to improve our biogeochemical models, more observational data from the bloom region north of South Georgia are required.
Shelf-sediment-derived Fe and Fe released from Antarctic krill significantly contribute to the DFe distribution in the shelf waters around South Georgia. Nevertheless, DFe enriched in shelf waters are not effectively advected to the phytoplankton bloom region downstream of the island. Together with other Fe supplies – such as aeolian dust, deep winter mixing, and diapycnal mixing – the horizontal advection contributes only ∼30 % to the Fe requirements of a phytoplankton bloom downstream of South Georgia. We therefore hypothesize that the majority of the Fe is derived from remineralization of Fe-enriched phytoplankton cells and biogenic particles that are transported with the water masses into the bloom region.
While we highlight the importance of grazers and the cycling of various particulate Fe phases in the Fe fertilization of the South Georgia bloom, more work is needed to clarify the transport mechanisms of dissolved and particulate Fe.
The entire data set discussed in the paper is provided in the manuscript (Tables 1, 2, 4, and 5) and Supplement (Table S1 and S2).
The supplement related to this article is available online at: https://doi.org/10.5194/bg-15-4973-2018-supplement.
CS, KS, EPA, SF, and AAt designed the experiments for JC247. CS, MDP, and AAt performed the sampling and krill incubation experiments during JC247. CS and MC analysed the trace metal samples at NOCS. EPA sampled the seawater during JC274. Samples from JC274 were analysed by CS and MC. AAq, WBH, and RAM designed the experiments for JR55, and AAq analysed the samples. CS prepared the manuscript with contributions from all co-authors.
The authors declare that they have no conflict of interest.
We would like to thank the officers and crew of RRS James Clark Ross for assistance with the
pelagic sampling and those of RRS James Cook for the benthic coring. In addition, we
thank the two anonymous reviewers for reviewing the manuscript. This work
forms part of the NERC-AFI grant AFI9/07 to Angus Atkinson and Eric P. Achterberg (NE/F01547X/1). Rachel A.
Mills was funded by NERC grants NE/01249X/1 and NE/H004394/1. William B. Homoky was supported by
NERC fellowship NE/K009532/1.
Edited by: Laurent Bopp
Reviewed by: two anonymous referees
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