Effects of sea animal colonization on the coupling between dynamics and activity of soil ammonia-oxidizing bacteria and archaea in maritime Antarctica

Wang, Qing; Zhu, Renbin; Zheng, Yanling; Bao, Tao; Hou, Lijun

doi:https://doi.org/10.5194/bg-16-4113-2019

Articles | Volume 16, issue 20

https://doi.org/10.5194/bg-16-4113-2019

© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.

https://doi.org/10.5194/bg-16-4113-2019

© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 16, issue 20

Research article

|

28 Oct 2019

Research article |

| 28 Oct 2019

Effects of sea animal colonization on the coupling between dynamics and activity of soil ammonia-oxidizing bacteria and archaea in maritime Antarctica

Qing Wang, Renbin Zhu, Yanling Zheng, Tao Bao, and Lijun Hou

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Final revised paper (published on 28 Oct 2019)
Supplement to the final revised paper
Preprint (discussion started on 01 Apr 2019)
Supplement to the preprint

Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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- Supplement

RC1: 'Effects of Sea Animal Colonization on the Coupling between Dynamics and Activity of Soil Ammonia-oxidizing Bacteria and Archaea in Maritime Antarctica', Anonymous Referee #1, 19 Apr 2019
- AC1: 'Reply to review #1', Renbin Zhu, 04 Jul 2019
  - AC2: 'revised manuscript & Supplimentary Material', Renbin Zhu, 04 Jul 2019
RC2: 'Comments on manuscript bg-2019-114', Anonymous Referee #2, 07 Jun 2019
- AC3: 'Response to Referee #2', Renbin Zhu, 04 Jul 2019
  - AC4: 'Revised manuscript & Supplimentary Material', Renbin Zhu, 04 Jul 2019

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

ED: Reconsider after major revisions (22 Jul 2019) by Denise Akob

AR by Renbin Zhu on behalf of the Authors (05 Aug 2019) Author's response Manuscript

ED: Referee Nomination & Report Request started (12 Aug 2019) by Denise Akob

RR by Anonymous Referee #2 (13 Aug 2019)

Suggestions for revision or reasons for rejection

I am happy with all the changes the authors made in the revised version of the manuscript. Below are just some language corrections and suggestions which will further improve the manuscript:

Line 11: should be “animals” instead of “animal”
Line 19: it should be “increased” instead of “increases”
Line 25: I suggest to change to “suggested that AOB play a more important role in nitrification in animal colony soils”
Line 58: delete “the
Line 85: change to “potential activity”
Line 96: delete “an”
Lines 244-255: I suggest to change to “AOB amoA gene abundances were significantly higher than AOA amoA gene abundances”
Line 246: here as well: “amoA gene abundances”
Line 251: better: “Maximum AOA amoA gene abundances”
Line 259: better: “Unlike AOA amoA gene abundances, AOB amoA genes showed..
Line 260: delete “the”
Line 288: I suggest: “The higher abundance of AOB compared to AOA..”
Lines 304-305: better: “AOB amoA gene diversity was generally higher compared to AOA”
Line 306: better: “AOA amoA gene diversity was higher in PL and MS than in BS, whereas AOB amoA gene diversity was higher in…”
Line 311: phylogenetic analysis was correct
Lines 313 and 315: better: “Cluster I contained 11 OTUs…”
Lines 325-326: change back to previous sentence
Line 330: change “had” to “contained”
Line 331: change to “clusters II and III contained …”
Line 334: change “had” to “contained”
Line 335: delete “of”
Line 393: change to “their abundances were very similar to each other”
Line 403: better: “key factors in determining a predominance of AOB over AOA”
Line 411: better: “In addition to ammonium, phosphorus can typically be found in..”
Line 416: add “genes”after amoA
Line 451: better: “higher AOB abundances and..
Line 497: change to: “AOB amoA gene diversity was higher than that of AOA…”
Line 498: “genes” instead of “gene”
Line 520: change to “potential activity”
Lines 524-525, line 530: delete “the” before AOA/AOB copy numbers
Line 528: change to: “AOB amoA gene diversity was higher…”

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RR by Anonymous Referee #1 (03 Sep 2019)

ED: Publish subject to technical corrections (17 Sep 2019) by Denise Akob

AR by Renbin Zhu on behalf of the Authors (20 Sep 2019) Manuscript

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Short summary

We investigated abundance, potential activity, and diversity of soil ammonia-oxidizing archaea (AOA) and bacteria (AOB) in five Antarctic tundra patches, including penguin colony, seal colony, and tundra marsh. We have found (1) sea animal colonization increased AOB population size.; (2) AOB contributed to ammonia oxidation rates more than AOA in sea animal colonies; (3) community structures of AOB and AOA were closely related to soil biogeochemical processes associated with animal activities.