Articles | Volume 18, issue 15
https://doi.org/10.5194/bg-18-4629-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-18-4629-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
12 Aug 2021
Research article |
| 12 Aug 2021
| 12 Aug 2021
Comparing modified substrate-induced respiration with selective inhibition (SIRIN) and N2O isotope approaches to estimate fungal contribution to denitrification in three arable soils under anoxic conditions
Department of Soil System Science, Helmholtz Centre for Environmental Research – UFZ, Theodor-Lieser Str. 4, 06120 Halle, Germany
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Department of Crop Sciences, Institute of Grassland Science, University of Göttingen, von-Siebold-Str. 8, 37075 Göttingen, Germany
Traute-Heidi Anderson
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Heinz Flessa
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Anette Goeske
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Dominika Lewicka-Szczebak
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Institute of Geological Sciences, University of Wrocław, pl. M. Borna 9, 50-204 Wrocław, Poland
Nicole Wrage-Mönnig
Grassland and Fodder Sciences, Agricultural and Environmental Faculty, University of Rostock, Justus-Liebig-Weg 6, 18059 Rostock, Germany
Reinhard Well
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
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Cited
21 citations as recorded by crossref.
- The influence of soil acidification on N2O emissions derived from fungal and bacterial denitrification using dual isotopocule mapping and acetylene inhibition Q. Zheng et al. 10.1016/j.envpol.2022.119076
- Acidification Offset Warming-Induced Increase in N2O Production in Estuarine and Coastal Sediments X. Li et al. 10.1021/acs.est.3c10691
- Soil clay content determined the temperature response of N2O emissions derived from denitrification L. Jia et al. 10.1016/j.apsoil.2025.106500
- Aggravation of nitrous oxide emissions driven by burrowing crab activities in intertidal marsh soils: Mechanisms and environmental implications Z. An et al. 10.1016/j.soilbio.2022.108732
- Differential N2O-Producing Activity of Soil Fungi Across Agricultural Systems: High in Vegetable Fields and Vineyards, Low in Paddies S. Ma et al. 10.3390/nitrogen6030057
- Warming and redistribution of nitrogen inputs drive an increase in terrestrial nitrous oxide emission factor E. Harris et al. 10.1038/s41467-022-32001-z
- Increased Nitrogen Loading Facilitates Nitrous Oxide Production through Fungal and Chemodenitrification in Estuarine and Coastal Sediments X. Li et al. 10.1021/acs.est.2c06602
- Denitrification in Agricultural Soils – Integrated control and Modelling at various scales (DASIM) K. Kleineidam et al. 10.1007/s00374-025-01894-5
- Technical note: A Bayesian mixing model to unravel isotopic data and quantify trace gas production and consumption pathways for time series data – Time-resolved FRactionation And Mixing Evaluation (TimeFRAME) E. Harris et al. 10.5194/bg-21-3641-2024
- Distribution and Environmental Drivers of Fungal Denitrifiers in Global Soils Y. Bösch et al. 10.1128/spectrum.00061-23
- Combining the 15N Gas Flux Method and N2O Isotopocule Data for the Determination of Soil Microbial N2O Sources G. Micucci et al. 10.1002/rcm.9971
- Simulated warming and low O2 promote N2O and N2 emissions in subtropical montane forest soil X. Yang et al. 10.1007/s11368-022-03234-8
- Effect of agricultural management system (“cash crop”, “livestock” and “climate optimized”) on nitrous oxide and ammonia emissions R. Well et al. 10.1007/s00374-024-01843-8
- Earthworm-controlled CO2, N2O and N2 emissions from a silt loam soil amended with senescent litter of two energy crops (Silphium perfoliatum and Zea mays) L. Wöhl et al. 10.1007/s11104-025-07696-w
- Microbial nitrogen transformations tracked by natural abundance isotope studies and microbiological methods: A review S. Deb et al. 10.1016/j.scitotenv.2024.172073
- Hydrologic Connectivity Regulates Riverine N2O Sources and Dynamics M. Hu et al. 10.1021/acs.est.4c01285
- The distribution of particulate organic matter in the heterogeneous soil matrix - Balancing between aerobic respiration and denitrification M. Lucas et al. 10.1016/j.scitotenv.2024.175383
- Partitioning denitrification pathways in N2O emissions from re-flooded dry paddy soils Y. Tang et al. 10.1007/s10533-024-01164-w
- Glucose Elevates N2O Emissions by Promoting Fungal and Incomplete Denitrification in North China Vegetable Soils Q. Zheng et al. 10.3390/agronomy15092127
- N2O emission dynamics along an intertidal elevation gradient in a subtropical estuary: Importance of N2O consumption D. Gao et al. 10.1016/j.envres.2021.112432
- The 15N-Gas flux method for quantifying denitrification in soil: Current progress and future directions G. Micucci et al. 10.1016/j.soilbio.2023.109108
21 citations as recorded by crossref.
- The influence of soil acidification on N2O emissions derived from fungal and bacterial denitrification using dual isotopocule mapping and acetylene inhibition Q. Zheng et al. 10.1016/j.envpol.2022.119076
- Acidification Offset Warming-Induced Increase in N2O Production in Estuarine and Coastal Sediments X. Li et al. 10.1021/acs.est.3c10691
- Soil clay content determined the temperature response of N2O emissions derived from denitrification L. Jia et al. 10.1016/j.apsoil.2025.106500
- Aggravation of nitrous oxide emissions driven by burrowing crab activities in intertidal marsh soils: Mechanisms and environmental implications Z. An et al. 10.1016/j.soilbio.2022.108732
- Differential N2O-Producing Activity of Soil Fungi Across Agricultural Systems: High in Vegetable Fields and Vineyards, Low in Paddies S. Ma et al. 10.3390/nitrogen6030057
- Warming and redistribution of nitrogen inputs drive an increase in terrestrial nitrous oxide emission factor E. Harris et al. 10.1038/s41467-022-32001-z
- Increased Nitrogen Loading Facilitates Nitrous Oxide Production through Fungal and Chemodenitrification in Estuarine and Coastal Sediments X. Li et al. 10.1021/acs.est.2c06602
- Denitrification in Agricultural Soils – Integrated control and Modelling at various scales (DASIM) K. Kleineidam et al. 10.1007/s00374-025-01894-5
- Technical note: A Bayesian mixing model to unravel isotopic data and quantify trace gas production and consumption pathways for time series data – Time-resolved FRactionation And Mixing Evaluation (TimeFRAME) E. Harris et al. 10.5194/bg-21-3641-2024
- Distribution and Environmental Drivers of Fungal Denitrifiers in Global Soils Y. Bösch et al. 10.1128/spectrum.00061-23
- Combining the 15N Gas Flux Method and N2O Isotopocule Data for the Determination of Soil Microbial N2O Sources G. Micucci et al. 10.1002/rcm.9971
- Simulated warming and low O2 promote N2O and N2 emissions in subtropical montane forest soil X. Yang et al. 10.1007/s11368-022-03234-8
- Effect of agricultural management system (“cash crop”, “livestock” and “climate optimized”) on nitrous oxide and ammonia emissions R. Well et al. 10.1007/s00374-024-01843-8
- Earthworm-controlled CO2, N2O and N2 emissions from a silt loam soil amended with senescent litter of two energy crops (Silphium perfoliatum and Zea mays) L. Wöhl et al. 10.1007/s11104-025-07696-w
- Microbial nitrogen transformations tracked by natural abundance isotope studies and microbiological methods: A review S. Deb et al. 10.1016/j.scitotenv.2024.172073
- Hydrologic Connectivity Regulates Riverine N2O Sources and Dynamics M. Hu et al. 10.1021/acs.est.4c01285
- The distribution of particulate organic matter in the heterogeneous soil matrix - Balancing between aerobic respiration and denitrification M. Lucas et al. 10.1016/j.scitotenv.2024.175383
- Partitioning denitrification pathways in N2O emissions from re-flooded dry paddy soils Y. Tang et al. 10.1007/s10533-024-01164-w
- Glucose Elevates N2O Emissions by Promoting Fungal and Incomplete Denitrification in North China Vegetable Soils Q. Zheng et al. 10.3390/agronomy15092127
- N2O emission dynamics along an intertidal elevation gradient in a subtropical estuary: Importance of N2O consumption D. Gao et al. 10.1016/j.envres.2021.112432
- The 15N-Gas flux method for quantifying denitrification in soil: Current progress and future directions G. Micucci et al. 10.1016/j.soilbio.2023.109108
Latest update: 30 Oct 2025
Short summary
This is the first experimental setup combining a complex set of methods (microbial inhibitors and isotopic approaches) to differentiate between N2O produced by fungi or bacteria during denitrification in three soils. Quantifying the fungal fraction with inhibitors was not successful due to large amounts of uninhibited N2O production. All successful methods suggested a small or missing fungal contribution. Artefacts occurring with microbial inhibition to determine N2O fluxes are discussed.
This is the first experimental setup combining a complex set of methods (microbial inhibitors...
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