Articles | Volume 18, issue 15
Biogeosciences, 18, 4629–4650, 2021
https://doi.org/10.5194/bg-18-4629-2021
Biogeosciences, 18, 4629–4650, 2021
https://doi.org/10.5194/bg-18-4629-2021
Research article
12 Aug 2021
Research article | 12 Aug 2021

Comparing modified substrate-induced respiration with selective inhibition (SIRIN) and N2O isotope approaches to estimate fungal contribution to denitrification in three arable soils under anoxic conditions

Lena Rohe et al.

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Latest update: 08 Aug 2022
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Short summary
This is the first experimental setup combining a complex set of methods (microbial inhibitors and isotopic approaches) to differentiate between N2O produced by fungi or bacteria during denitrification in three soils. Quantifying the fungal fraction with inhibitors was not successful due to large amounts of uninhibited N2O production. All successful methods suggested a small or missing fungal contribution. Artefacts occurring with microbial inhibition to determine N2O fluxes are discussed.
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