Articles | Volume 18, issue 15
https://doi.org/10.5194/bg-18-4629-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-18-4629-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
12 Aug 2021
Research article |
| 12 Aug 2021
| 12 Aug 2021
Comparing modified substrate-induced respiration with selective inhibition (SIRIN) and N2O isotope approaches to estimate fungal contribution to denitrification in three arable soils under anoxic conditions
Department of Soil System Science, Helmholtz Centre for Environmental Research – UFZ, Theodor-Lieser Str. 4, 06120 Halle, Germany
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Department of Crop Sciences, Institute of Grassland Science, University of Göttingen, von-Siebold-Str. 8, 37075 Göttingen, Germany
Traute-Heidi Anderson
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Heinz Flessa
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Anette Goeske
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Dominika Lewicka-Szczebak
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Institute of Geological Sciences, University of Wrocław, pl. M. Borna 9, 50-204 Wrocław, Poland
Nicole Wrage-Mönnig
Grassland and Fodder Sciences, Agricultural and Environmental Faculty, University of Rostock, Justus-Liebig-Weg 6, 18059 Rostock, Germany
Reinhard Well
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
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Short summary
This is the first experimental setup combining a complex set of methods (microbial inhibitors and isotopic approaches) to differentiate between N2O produced by fungi or bacteria during denitrification in three soils. Quantifying the fungal fraction with inhibitors was not successful due to large amounts of uninhibited N2O production. All successful methods suggested a small or missing fungal contribution. Artefacts occurring with microbial inhibition to determine N2O fluxes are discussed.
This is the first experimental setup combining a complex set of methods (microbial inhibitors...
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