Articles | Volume 18, issue 15
https://doi.org/10.5194/bg-18-4629-2021
https://doi.org/10.5194/bg-18-4629-2021
Research article
 | 
12 Aug 2021
Research article |  | 12 Aug 2021

Comparing modified substrate-induced respiration with selective inhibition (SIRIN) and N2O isotope approaches to estimate fungal contribution to denitrification in three arable soils under anoxic conditions

Lena Rohe, Traute-Heidi Anderson, Heinz Flessa, Anette Goeske, Dominika Lewicka-Szczebak, Nicole Wrage-Mönnig, and Reinhard Well

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Cited articles

Aerssens, E., Tiedje, J. M., and Averill, B. A.: Isotope labeling studies on the mechanisms of N-bond formation in denitrification, J. Biol. Chem., 261, 9652–9656, 1986. 
Anderson, J. P. E. and Domsch, K. H.: Quantification of bacterial and fungal contributions to soil respiration, Arch Mikrobiol., 93, 113–127, https://doi.org/10.1007/BF00424942, 1973. 
Anderson, J. P. E. and Domsch, K. H.: Measurement of bacterial and fungal contributions to respiration of selected agricultural and forest soil, Can. J. Microbiol., 21, 314–322, https://doi.org/10.1139/m75-045, 1975. 
Anderson, J. P. E. and Domsch, K. H.: Physiological method for quantitative measurement of microbial biomass in soils, Soil Biol. Biochem., 10, 215–221, https://doi.org/10.1016/0038-0717(78)90099-8, 1978. 
Bergsma, T. T., Ostrom, N. E., Emmons, M., and Robertson, G. P.: Measuring simultaneous fluxes from soil of N2O and N2 in the field using the 15N-Gas “nonequilihrium” technique, Environ. Sci. Technol., 35, 4307–4312, https://doi.org/10.1021/es010885u, 2001. 
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Short summary
This is the first experimental setup combining a complex set of methods (microbial inhibitors and isotopic approaches) to differentiate between N2O produced by fungi or bacteria during denitrification in three soils. Quantifying the fungal fraction with inhibitors was not successful due to large amounts of uninhibited N2O production. All successful methods suggested a small or missing fungal contribution. Artefacts occurring with microbial inhibition to determine N2O fluxes are discussed.
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