Articles | Volume 18, issue 15
https://doi.org/10.5194/bg-18-4629-2021
https://doi.org/10.5194/bg-18-4629-2021
Research article
 | 
12 Aug 2021
Research article |  | 12 Aug 2021

Comparing modified substrate-induced respiration with selective inhibition (SIRIN) and N2O isotope approaches to estimate fungal contribution to denitrification in three arable soils under anoxic conditions

Lena Rohe, Traute-Heidi Anderson, Heinz Flessa, Anette Goeske, Dominika Lewicka-Szczebak, Nicole Wrage-Mönnig, and Reinhard Well

Related authors

Land use impact on carbon mineralization in well aerated soils is mainly explained by variations of particulate organic matter rather than of soil structure
Steffen Schlüter, Tim Roussety, Lena Rohe, Vusal Guliyev, Evgenia Blagodatskaya, and Thomas Reitz
SOIL, 8, 253–267, https://doi.org/10.5194/soil-8-253-2022,https://doi.org/10.5194/soil-8-253-2022, 2022
Short summary
Denitrification in soil as a function of oxygen availability at the microscale
Lena Rohe, Bernd Apelt, Hans-Jörg Vogel, Reinhard Well, Gi-Mick Wu, and Steffen Schlüter
Biogeosciences, 18, 1185–1201, https://doi.org/10.5194/bg-18-1185-2021,https://doi.org/10.5194/bg-18-1185-2021, 2021
Short summary

Related subject area

Biogeochemistry: Stable Isotopes & Other Tracers
Sea ice and mixed layer depth influence on nitrate depletion and associated isotopic effects in the Drake Passage–Weddell Sea region, Southern Ocean
Aymeric P. M. Servettaz, Yuta Isaji, Chisato Yoshikawa, Yanghee Jang, Boo-Keun Khim, Yeongjun Ryu, Daniel M. Sigman, Nanako O. Ogawa, Francisco J. Jiménez-Espejo, and Naohiko Ohkouchi
Biogeosciences, 22, 2239–2260, https://doi.org/10.5194/bg-22-2239-2025,https://doi.org/10.5194/bg-22-2239-2025, 2025
Short summary
Stable iron isotope signals indicate a “pseudo-abiotic” process driving deep iron release in methanic sediments
Susann Henkel, Bo Liu, Michael Staubwasser, Simone A. Kasemann, Anette Meixner, David A. Aromokeye, Michael W. Friedrich, and Sabine Kasten
Biogeosciences, 22, 1673–1696, https://doi.org/10.5194/bg-22-1673-2025,https://doi.org/10.5194/bg-22-1673-2025, 2025
Short summary
Triple oxygen isotope evidence for the pathway of nitrous oxide production in a forested soil with increased emission on rainy days
Weitian Ding, Urumu Tsunogai, Tianzheng Huang, Takashi Sambuichi, Wenhua Ruan, Masanori Ito, Hao Xu, Yongwon Kim, and Fumiko Nakagawa
EGUsphere, https://doi.org/10.21203/rs.3.rs-4264720/v2,https://doi.org/10.21203/rs.3.rs-4264720/v2, 2025
Short summary
Previous integrated or organic farming affects productivity and ecosystem N balance rather than fertilizer 15N allocation to plants and soil, leaching, or gaseous emissions (NH3, N2O, and N2)
Fawad Khan, Samuel Franco Luesma, Frederik Hartmann, Michael Dannenmann, Rainer Gasche, Clemens Scheer, Andreas Gattinger, Wiebke Niether, Elizabeth Gachibu Wangari, Ricky Mwangada Mwanake, Ralf Kiese, and Benjamin Wolf
EGUsphere, https://doi.org/10.5194/egusphere-2025-292,https://doi.org/10.5194/egusphere-2025-292, 2025
Short summary
How long does carbon stay in a near-pristine central Amazon forest? An empirical estimate with radiocarbon
Ingrid Chanca, Ingeborg Levin, Susan Trumbore, Kita Macario, Jost Lavric, Carlos Alberto Quesada, Alessandro Carioca de Araújo, Cléo Quaresma Dias Júnior, Hella van Asperen, Samuel Hammer, and Carlos A. Sierra
Biogeosciences, 22, 455–472, https://doi.org/10.5194/bg-22-455-2025,https://doi.org/10.5194/bg-22-455-2025, 2025
Short summary

Cited articles

Aerssens, E., Tiedje, J. M., and Averill, B. A.: Isotope labeling studies on the mechanisms of N-bond formation in denitrification, J. Biol. Chem., 261, 9652–9656, 1986. 
Anderson, J. P. E. and Domsch, K. H.: Quantification of bacterial and fungal contributions to soil respiration, Arch Mikrobiol., 93, 113–127, https://doi.org/10.1007/BF00424942, 1973. 
Anderson, J. P. E. and Domsch, K. H.: Measurement of bacterial and fungal contributions to respiration of selected agricultural and forest soil, Can. J. Microbiol., 21, 314–322, https://doi.org/10.1139/m75-045, 1975. 
Anderson, J. P. E. and Domsch, K. H.: Physiological method for quantitative measurement of microbial biomass in soils, Soil Biol. Biochem., 10, 215–221, https://doi.org/10.1016/0038-0717(78)90099-8, 1978. 
Bergsma, T. T., Ostrom, N. E., Emmons, M., and Robertson, G. P.: Measuring simultaneous fluxes from soil of N2O and N2 in the field using the 15N-Gas “nonequilihrium” technique, Environ. Sci. Technol., 35, 4307–4312, https://doi.org/10.1021/es010885u, 2001. 
Download
Short summary
This is the first experimental setup combining a complex set of methods (microbial inhibitors and isotopic approaches) to differentiate between N2O produced by fungi or bacteria during denitrification in three soils. Quantifying the fungal fraction with inhibitors was not successful due to large amounts of uninhibited N2O production. All successful methods suggested a small or missing fungal contribution. Artefacts occurring with microbial inhibition to determine N2O fluxes are discussed.
Share
Altmetrics
Final-revised paper
Preprint