Articles | Volume 18, issue 15
https://doi.org/10.5194/bg-18-4629-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-18-4629-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
12 Aug 2021
Research article |
| 12 Aug 2021
| 12 Aug 2021
Comparing modified substrate-induced respiration with selective inhibition (SIRIN) and N2O isotope approaches to estimate fungal contribution to denitrification in three arable soils under anoxic conditions
Department of Soil System Science, Helmholtz Centre for Environmental Research – UFZ, Theodor-Lieser Str. 4, 06120 Halle, Germany
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Department of Crop Sciences, Institute of Grassland Science, University of Göttingen, von-Siebold-Str. 8, 37075 Göttingen, Germany
Traute-Heidi Anderson
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Heinz Flessa
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Anette Goeske
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Dominika Lewicka-Szczebak
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Institute of Geological Sciences, University of Wrocław, pl. M. Borna 9, 50-204 Wrocław, Poland
Nicole Wrage-Mönnig
Grassland and Fodder Sciences, Agricultural and Environmental Faculty, University of Rostock, Justus-Liebig-Weg 6, 18059 Rostock, Germany
Reinhard Well
Thünen Institute of Climate-Smart Agriculture, Bundesallee 65, 38116 Braunschweig, Germany
Related authors
Steffen Schlüter, Tim Roussety, Lena Rohe, Vusal Guliyev, Evgenia Blagodatskaya, and Thomas Reitz
SOIL, 8, 253–267, https://doi.org/10.5194/soil-8-253-2022, https://doi.org/10.5194/soil-8-253-2022, 2022
Short summary
Short summary
We combined microstructure analysis via X-ray CT with carbon mineralization analysis via respirometry of intact soil cores from different land uses. We found that the amount of particulate organic matter (POM) exerted a dominant control on carbon mineralization in well-aerated topsoils, whereas soil moisture and macroporosity did not play role. This is because carbon mineralization mainly occurs in microbial hotspots around degrading POM, where it is decoupled from conditions of the bulk soil.
Lena Rohe, Bernd Apelt, Hans-Jörg Vogel, Reinhard Well, Gi-Mick Wu, and Steffen Schlüter
Biogeosciences, 18, 1185–1201, https://doi.org/10.5194/bg-18-1185-2021, https://doi.org/10.5194/bg-18-1185-2021, 2021
Short summary
Short summary
Total denitrification, i.e. N2O and (N2O + N2) fluxes, of repacked soil cores were analysed for different combinations of soils and water contents. Prediction accuracy of (N2O + N2) fluxes was highest with combined proxies for oxygen demand (CO2 flux) and oxygen supply (anaerobic soil volume fraction). Knowledge of denitrification completeness (product ratio) improved N2O predictions. Substitutions with cheaper proxies (soil organic matter, empirical diffusivity) reduced prediction accuracy.
Eliza Jean Harris, Philipp Fischer, Maciej P. Lewicki, Dominika Lewicka-Szczebak, Stephen J. Harris, and Fernando Perez-Cruz
EGUsphere, https://doi.org/10.5194/egusphere-2023-2836, https://doi.org/10.5194/egusphere-2023-2836, 2023
Short summary
Short summary
Greenhouse gases are produced and consumed via a number of pathways. Quantifying these pathways helps reduce the climate and environmental footprint of anthropogenic activities. The contribution of different pathways can be estimated from the isotopic composition, which acts as a "fingerprint" for these pathways. Interpreting this data is challenging. We have developed the TimeFRAME model to simplify interpretation, and estimate the contribution of different pathways and their uncertainty.
Steffen Schlüter, Tim Roussety, Lena Rohe, Vusal Guliyev, Evgenia Blagodatskaya, and Thomas Reitz
SOIL, 8, 253–267, https://doi.org/10.5194/soil-8-253-2022, https://doi.org/10.5194/soil-8-253-2022, 2022
Short summary
Short summary
We combined microstructure analysis via X-ray CT with carbon mineralization analysis via respirometry of intact soil cores from different land uses. We found that the amount of particulate organic matter (POM) exerted a dominant control on carbon mineralization in well-aerated topsoils, whereas soil moisture and macroporosity did not play role. This is because carbon mineralization mainly occurs in microbial hotspots around degrading POM, where it is decoupled from conditions of the bulk soil.
Balázs Grosz, Reinhard Well, Rene Dechow, Jan Reent Köster, Mohammad Ibrahim Khalil, Simone Merl, Andreas Rode, Bianca Ziehmer, Amanda Matson, and Hongxing He
Biogeosciences, 18, 5681–5697, https://doi.org/10.5194/bg-18-5681-2021, https://doi.org/10.5194/bg-18-5681-2021, 2021
Short summary
Short summary
To assure quality predictions biogeochemical models must be current. We use data measured using novel incubation methods to test the denitrification sub-modules of three models. We aim to identify limitations in the denitrification modeling to inform next steps for development. Several areas are identified, most urgently improved denitrification control parameters and further testing with high-temporal-resolution datasets. Addressing these would significantly improve denitrification modeling.
Lena Rohe, Bernd Apelt, Hans-Jörg Vogel, Reinhard Well, Gi-Mick Wu, and Steffen Schlüter
Biogeosciences, 18, 1185–1201, https://doi.org/10.5194/bg-18-1185-2021, https://doi.org/10.5194/bg-18-1185-2021, 2021
Short summary
Short summary
Total denitrification, i.e. N2O and (N2O + N2) fluxes, of repacked soil cores were analysed for different combinations of soils and water contents. Prediction accuracy of (N2O + N2) fluxes was highest with combined proxies for oxygen demand (CO2 flux) and oxygen supply (anaerobic soil volume fraction). Knowledge of denitrification completeness (product ratio) improved N2O predictions. Substitutions with cheaper proxies (soil organic matter, empirical diffusivity) reduced prediction accuracy.
Dominika Lewicka-Szczebak, Maciej Piotr Lewicki, and Reinhard Well
Biogeosciences, 17, 5513–5537, https://doi.org/10.5194/bg-17-5513-2020, https://doi.org/10.5194/bg-17-5513-2020, 2020
Short summary
Short summary
We present the first validation of N2O isotopic approaches for estimating N2O source pathways and N2O reduction. These approaches are widely used for tracing soil nitrogen cycling, but the results of these estimations are very uncertain. Here we report the results from parallel treatments allowing for precise validation of these approaches, and we propose the best strategies for results interpretation, including the new idea of an isotope model integrating three isotopic signatures of N2O.
Dominika Lewicka-Szczebak and Reinhard Well
SOIL, 6, 145–152, https://doi.org/10.5194/soil-6-145-2020, https://doi.org/10.5194/soil-6-145-2020, 2020
Short summary
Short summary
This study aimed at comparison of various experimental strategies for incubating soil samples to determine the N2 flux. Such experiments require addition of isotope tracer, i.e. nitrogen fertilizer enriched in heavy nitrogen isotopes (15N). Here we compared the impact of soil homogenization and mixing with the tracer and tracer injection to the intact soil cores. The results are well comparable: both techniques would provide similar conclusions on the magnitude of N2 flux.
Pauline Sophie Rummel, Birgit Pfeiffer, Johanna Pausch, Reinhard Well, Dominik Schneider, and Klaus Dittert
Biogeosciences, 17, 1181–1198, https://doi.org/10.5194/bg-17-1181-2020, https://doi.org/10.5194/bg-17-1181-2020, 2020
Short summary
Short summary
Chemical composition of plant litter controls C availability for biological N transformation processes in soil. In this study, we showed that easily degradable maize shoots stimulated microbial respiration and mineralization leading to high N2O formation in litter-associated hot spots. A higher share of slowly degradable C compounds and lower concentrations of water-soluble N restricted N2O emissions from maize roots. Bacterial community structure reflected degradability of maize litter.
Reinhard Well, Martin Maier, Dominika Lewicka-Szczebak, Jan-Reent Köster, and Nicolas Ruoss
Biogeosciences, 16, 2233–2246, https://doi.org/10.5194/bg-16-2233-2019, https://doi.org/10.5194/bg-16-2233-2019, 2019
Short summary
Short summary
Denitrification is a key process in the soil nitrogen cycle but poorly investigated due to methodical limitations. The 15N gas flux method is currently the only approach allowing field measurement of denitrification but was subject to bias due to unaccounted fluxes of 15N-labelled gaseous denitrification products to the subsoil. We used field flux experiments and diffusion–reaction modelling to estimate this source of error and developed an approach to correct denitrification rates.
Elizabeth Verhoeven, Matti Barthel, Longfei Yu, Luisella Celi, Daniel Said-Pullicino, Steven Sleutel, Dominika Lewicka-Szczebak, Johan Six, and Charlotte Decock
Biogeosciences, 16, 383–408, https://doi.org/10.5194/bg-16-383-2019, https://doi.org/10.5194/bg-16-383-2019, 2019
Short summary
Short summary
This study utilized state-of-the-art measurements of nitrogen isotopes to evaluate nitrogen cycling and to assess the biological sources of the potent greenhouse gas, N2O, in response to water-saving practices in rice systems. Water-saving practices did emit more N2O, and high N2O production had a lower 15N isotope signature. Modeling and visual interpretation indicate that these emissions mostly came from denitrification or nitrifier denitrification, controlled upstream by nitrification rates.
Laura Maritza Cardenas, Roland Bol, Dominika Lewicka-Szczebak, Andrew Stuart Gregory, Graham Peter Matthews, William Richard Whalley, Thomas Henry Misselbrook, David Scholefield, and Reinhard Well
Biogeosciences, 14, 4691–4710, https://doi.org/10.5194/bg-14-4691-2017, https://doi.org/10.5194/bg-14-4691-2017, 2017
Short summary
Short summary
A laboratory incubation was carried out at different soil moisture levels to measure emissions of nitrogen gases and the isotopomers (position of 15N) of nitrous oxide. Flux variability was larger in drier conditions, probably due to nutrient distribution heterogeneity created from soil cracks and consequently nutrient hot spots. Denitrification was the main source of fluxes at higher moisture, but nitrification could have occurred under drier conditions (although moisture was still high).
Sebastian Rainer Fiedler, Jürgen Augustin, Nicole Wrage-Mönnig, Gerald Jurasinski, Bertram Gusovius, and Stephan Glatzel
SOIL, 3, 161–176, https://doi.org/10.5194/soil-3-161-2017, https://doi.org/10.5194/soil-3-161-2017, 2017
Short summary
Short summary
Injection of biogas digestates (BDs) is suspected to increase losses of N2O and thus to counterbalance prevented NH3 emissions. We determined N2O and N2 losses after mixing high concentrations of BD into two soils by an incubation under an artificial helium–oxygen atmosphere. Emissions did not increase with the application rate of BD, probably due to an inhibitory effect of the high NH4+ content in BD on nitrification. However, cumulated gaseous N losses may effectively offset NH3 reductions.
Christian Brümmer, Bjarne Lyshede, Dirk Lempio, Jean-Pierre Delorme, Jeremy J. Rüffer, Roland Fuß, Antje M. Moffat, Miriam Hurkuck, Andreas Ibrom, Per Ambus, Heinz Flessa, and Werner L. Kutsch
Biogeosciences, 14, 1365–1381, https://doi.org/10.5194/bg-14-1365-2017, https://doi.org/10.5194/bg-14-1365-2017, 2017
Short summary
Short summary
We present a novel chamber design for measuring soil–atmosphere N2O fluxes and compare the performance of a commonly applied gas chromatography (GC) setup with laser-based (QCL) concentration detection. While GC was still a useful method for longer-term investigations, we found that closure times of 10 min and sampling every 5 s is sufficient when using a QCL system. Further, extremely low standard errors (< 2 % of flux value) were observed regardless of linear or exponential flux calculation.
Dominika Lewicka-Szczebak, Jürgen Augustin, Anette Giesemann, and Reinhard Well
Biogeosciences, 14, 711–732, https://doi.org/10.5194/bg-14-711-2017, https://doi.org/10.5194/bg-14-711-2017, 2017
Short summary
Short summary
The consumption of the greenhouse gas nitrous oxide (N2O) by its reduction to dinitrogen via microbial denitrification in soil is poorly quantified. This precludes improvements in nitrogen (N) efficiency in agricultural ecosystems and mitigation of N losses to the environment including N2O fluxes. We present a laboratory evaluation for the determination of N2O reduction based on stable isotope values of soil-emitted N2O as a new approach to determine N2O reduction in the field studies.
Dominika Lewicka-Szczebak, Jens Dyckmans, Jan Kaiser, Alina Marca, Jürgen Augustin, and Reinhard Well
Biogeosciences, 13, 1129–1144, https://doi.org/10.5194/bg-13-1129-2016, https://doi.org/10.5194/bg-13-1129-2016, 2016
Short summary
Short summary
Oxygen isotopic signatures of N2O are formed in complex multistep enzymatic reactions and depend on isotopic fractionation during enzymatic reduction of nitrate to N2O and on the oxygen isotope exchange with soil water. We propose a new method for quantification of oxygen isotope exchange, with simultaneous determination of oxygen isotopic signatures, to decipher the mechanism of oxygen isotopic fractionation. We indicate the differences between fractionation mechanisms by various pathways.
W. Eschenbach, R. Well, and W. Walther
Biogeosciences, 12, 2327–2346, https://doi.org/10.5194/bg-12-2327-2015, https://doi.org/10.5194/bg-12-2327-2015, 2015
Related subject area
Biogeochemistry: Stable Isotopes & Other Tracers
Atmospheric O2 and CO2 measurements at a single height provide weak constraint on surface carbon exchange
Technical Note: TimeFRAME – A Bayesian Mixing Model to Unravel Isotopic Data and Quantify Trace Gas Production and Consumption Pathways for Timeseries Data
Climatic controls on leaf wax hydrogen isotope ratios in terrestrial and marine sediments along a hyperarid-to-humid gradient
Fractionation of stable carbon isotopes during microbial propionate consumption in anoxic rice paddy soils
Sources and sinks of carbonyl sulfide inferred from tower and mobile atmospheric observations in the Netherlands
Downpour dynamics: outsized impacts of storm events on unprocessed atmospheric nitrate export in an urban watershed
The hidden role of dissolved organic carbon in the biogeochemical cycle of carbon in modern redox-stratified lakes
Biogeochemical processes captured by carbon isotopes in redox-stratified water columns: a comparative study of four modern stratified lakes along an alkalinity gradient
Partitioning of carbon export in the euphotic zone of the oligotrophic South China Sea
Determination of respiration and photosynthesis fractionation factors for atmospheric dioxygen inferred from a vegetation–soil–atmosphere analogue of the terrestrial biosphere in closed chambers
Permafrost degradation and nitrogen cycling in Arctic rivers: insights from stable nitrogen isotope studies
Neodymium budget in the Mediterranean Sea: evaluating the role of atmospheric dusts using a high-resolution dynamical-biogeochemical model
Nitrate isotope investigations reveal future impacts of climate change on nitrogen inputs and cycling in Arctic fjords: Kongsfjorden and Rijpfjorden (Svalbard)
Mineralization of autochthonous particulate organic carbon is a fast channel of organic matter turnover in Germany's largest drinking water reservoir
Carbon isotopic ratios of modern C3 and C4 vegetation on the Indian peninsula and changes along the plant–soil–river continuum – implications for vegetation reconstructions
Controls on nitrite oxidation in the upper Southern Ocean: insights from winter kinetics experiments in the Indian sector
Tracing the source of nitrate in a forested stream showing elevated concentrations during storm events
Intra-skeletal variability in phosphate oxygen isotope composition reveals regional heterothermies in marine vertebrates
Isotopic differences in soil–plant–atmosphere continuum composition and control factors of different vegetation zones on the northern slope of the Qilian Mountains
An analysis of the variability in δ13C in macroalgae from the Gulf of California: indicative of carbon concentration mechanisms and isotope discrimination during carbon assimilation
Summertime productivity and carbon export potential in the Weddell Sea, with a focus on the waters adjacent to Larsen C Ice Shelf
Particulate biogenic barium tracer of mesopelagic carbon remineralization in the Mediterranean Sea (PEACETIME project)
Hydrogen and carbon isotope fractionation factors of aerobic methane oxidation in deep-sea water
Host-influenced geochemical signature in the parasitic foraminifera Hyrrokkin sarcophaga
How are oxygen budgets influenced by dissolved iron and growth of oxygenic phototrophs in an iron-rich spring system? Initial results from the Espan Spring in Fürth, Germany
Stable isotope ratios in seawater nitrate reflect the influence of Pacific water along the northwest Atlantic margin
High-resolution 14C bomb peak dating and climate response analyses of subseasonal stable isotope signals in wood of the African baobab – a case study from Oman
Geographic variability in freshwater methane hydrogen isotope ratios and its implications for global isotopic source signatures
Seasonality of nitrogen sources, cycling, and loading in a New England river discerned from nitrate isotope ratios
Evaluating the response of δ13C in Haloxylon ammodendron, a dominant C4 species in Asian desert ecosystems, to water and nitrogen addition as well as the availability of its δ13C as an indicator of water use efficiency
Modern silicon dynamics of a small high-latitude subarctic lake
Radium-228-derived ocean mixing and trace element inputs in the South Atlantic
Nitrogen isotopic fractionations during nitric oxide production in an agricultural soil
Silicon uptake and isotope fractionation dynamics by crop species
Barium stable isotopes as a fingerprint of biological cycling in the Amazon River basin
Bottomland hardwood forest growth and stress response to hydroclimatic variation: evidence from dendrochronology and tree ring Δ13C values
N2O isotope approaches for source partitioning of N2O production and estimation of N2O reduction – validation with the 15N gas-flux method in laboratory and field studies
Technical note: Single-shell δ11B analysis of Cibicidoides wuellerstorfi using femtosecond laser ablation MC-ICPMS and secondary ion mass spectrometry
Biogeochemical evidence of anaerobic methane oxidation and anaerobic ammonium oxidation in a stratified lake using stable isotopes
Effects of 238U variability and physical transport on water column 234Th downward fluxes in the coastal upwelling system off Peru
Do degree and rate of silicate weathering depend on plant productivity?
Alpine Holocene tree-ring dataset: age-related trends in the stable isotopes of cellulose show species-specific patterns
Ideas and perspectives: The same carbon behaves like different elements – an insight into position-specific isotope distributions
Seasonal dynamics of the COS and CO2 exchange of a managed temperate grassland
Leaf-scale quantification of the effect of photosynthetic gas exchange on Δ17O of atmospheric CO2
The stable carbon isotope signature of methane produced by saprotrophic fungi
Understanding the effects of early degradation on isotopic tracers: implications for sediment source attribution using compound-specific isotope analysis (CSIA)
Oxygen isotope composition of waters recorded in carbonates in strong clumped and oxygen isotopic disequilibrium
Isotopic evidence for alteration of nitrous oxide emissions and producing pathways' contribution under nitrifying conditions
Trace element composition of size-fractionated suspended particulate matter samples from the Qatari Exclusive Economic Zone of the Arabian Gulf: the role of atmospheric dust
Kim A. P. Faassen, Jordi Vilà-Guerau de Arellano, Raquel González-Armas, Bert G. Heusinkveld, Ivan Mammarella, Wouter Peters, and Ingrid T. Luijkx
EGUsphere, https://doi.org/10.5194/egusphere-2023-2833, https://doi.org/10.5194/egusphere-2023-2833, 2023
Short summary
Short summary
The ratio between atmospheric O2 and CO2 can be used to characterise the carbon balance of the surface. By combining a model and observations from the Hyytiala forest (Finland), we show that using atmospheric O2 and CO2 measurements from a single height provides a weak constraint on the surface CO2 exchange, because large scale processes such as entrainment confound this signal. We therefore recommend to always use multiple heights of O2 and CO2 measurements to study surface CO2 exchange.
Eliza Jean Harris, Philipp Fischer, Maciej P. Lewicki, Dominika Lewicka-Szczebak, Stephen J. Harris, and Fernando Perez-Cruz
EGUsphere, https://doi.org/10.5194/egusphere-2023-2836, https://doi.org/10.5194/egusphere-2023-2836, 2023
Short summary
Short summary
Greenhouse gases are produced and consumed via a number of pathways. Quantifying these pathways helps reduce the climate and environmental footprint of anthropogenic activities. The contribution of different pathways can be estimated from the isotopic composition, which acts as a "fingerprint" for these pathways. Interpreting this data is challenging. We have developed the TimeFRAME model to simplify interpretation, and estimate the contribution of different pathways and their uncertainty.
Nestor Gaviria-Lugo, Charlotte Läuchli, Hella Wittmann, Anne Bernhardt, Patrick Frings, Mahyar Mohtadi, Oliver Rach, and Dirk Sachse
Biogeosciences, 20, 4433–4453, https://doi.org/10.5194/bg-20-4433-2023, https://doi.org/10.5194/bg-20-4433-2023, 2023
Short summary
Short summary
We analyzed how leaf wax hydrogen isotopes in continental and marine sediments respond to climate along one of the strongest aridity gradients in the world, from hyperarid to humid, along Chile. We found that under extreme aridity, the relationship between hydrogen isotopes in waxes and climate is non-linear, suggesting that we should be careful when reconstructing past hydrological changes using leaf wax hydrogen isotopes so as to avoid overestimating how much the climate has changed.
Ralf Conrad and Peter Claus
Biogeosciences, 20, 3625–3635, https://doi.org/10.5194/bg-20-3625-2023, https://doi.org/10.5194/bg-20-3625-2023, 2023
Short summary
Short summary
Knowledge of carbon isotope fractionation is important for the assessment of the pathways involved in the degradation of organic matter. Propionate is an important intermediate. In the presence of sulfate, it was degraded by Syntrophobacter species via acetate to CO2. In the absence of sulfate, it was mainly consumed by Smithella and methanogenic archaeal species via butyrate and acetate to CH4. However, stable carbon isotope fractionation during the degradation process was quite small.
Alessandro Zanchetta, Linda M. J. Kooijmans, Steven van Heuven, Andrea Scifo, Hubertus A. Scheeren, Ivan Mammarella, Ute Karstens, Jin Ma, Maarten Krol, and Huilin Chen
Biogeosciences, 20, 3539–3553, https://doi.org/10.5194/bg-20-3539-2023, https://doi.org/10.5194/bg-20-3539-2023, 2023
Short summary
Short summary
Carbonyl sulfide (COS) has been suggested as a tool to estimate carbon dioxide (CO2) uptake by plants during photosynthesis. However, understanding its sources and sinks is critical to preventing biases in this estimate. Combining observations and models, this study proves that regional sources occasionally influence the measurements at the 60 m tall Lutjewad tower (1 m a.s.l.; 53°24′ N, 6°21′ E) in the Netherlands. Moreover, it estimates nighttime COS fluxes to be −3.0 ± 2.6 pmol m−2 s−1.
Joel T. Bostic, David M. Nelson, and Keith N. Eshleman
Biogeosciences, 20, 2485–2498, https://doi.org/10.5194/bg-20-2485-2023, https://doi.org/10.5194/bg-20-2485-2023, 2023
Short summary
Short summary
Land-use changes can affect water quality. We used tracers of pollution sources and water flow paths to show that an urban watershed exports variable sources during storm events relative to a less developed watershed. Our results imply that changing precipitation patterns combined with increasing urbanization may alter sources of pollution in the future.
Robin Havas, Christophe Thomazo, Miguel Iniesto, Didier Jézéquel, David Moreira, Rosaluz Tavera, Jeanne Caumartin, Elodie Muller, Purificación López-García, and Karim Benzerara
Biogeosciences, 20, 2405–2424, https://doi.org/10.5194/bg-20-2405-2023, https://doi.org/10.5194/bg-20-2405-2023, 2023
Short summary
Short summary
Dissolved organic carbon (DOC) is a reservoir of prime importance in the C cycle of both continental and marine systems. It has also been suggested to influence the past Earth climate but is still poorly characterized in ancient-Earth-like environments. In this paper we show how DOC analyses from modern redox-stratified lakes can evidence specific metabolic reactions and environmental factors and how these can help us to interpret the C cycle of specific periods in the Earth's past.
Robin Havas, Christophe Thomazo, Miguel Iniesto, Didier Jézéquel, David Moreira, Rosaluz Tavera, Jeanne Caumartin, Elodie Muller, Purificación López-García, and Karim Benzerara
Biogeosciences, 20, 2347–2367, https://doi.org/10.5194/bg-20-2347-2023, https://doi.org/10.5194/bg-20-2347-2023, 2023
Short summary
Short summary
We describe the C cycle of four modern stratified water bodies from Mexico, a necessary step to better understand the C cycle of primitive-Earth-like environments, which were dominated by these kinds of conditions. We highlight the importance of local external factors on the C cycle of these systems. Notably, they influence the sensitivity of the carbonate record to environmental changes. We also show the strong C-cycle variability among these lakes and their organic C sediment record.
Yifan Ma, Kuanbo Zhou, Weifang Chen, Junhui Chen, Jin-Yu Terence Yang, and Minhan Dai
Biogeosciences, 20, 2013–2030, https://doi.org/10.5194/bg-20-2013-2023, https://doi.org/10.5194/bg-20-2013-2023, 2023
Short summary
Short summary
We distinguished particulate organic carbon (POC) export fluxes out of the nutrient-depleted layer (NDL) and the euphotic zone. The amount of POC export flux at the NDL base suggests that the NDL could be a hotspot of particle export. The substantial POC export flux at the NDL base challenges traditional concepts that the NDL was limited in terms of POC export. The dominant nutrient source for POC export fluxes should be subsurface nutrients, which was determined by 15N isotopic mass balance.
Clémence Paul, Clément Piel, Joana Sauze, Nicolas Pasquier, Frédéric Prié, Sébastien Devidal, Roxanne Jacob, Arnaud Dapoigny, Olivier Jossoud, Alexandru Milcu, and Amaëlle Landais
Biogeosciences, 20, 1047–1062, https://doi.org/10.5194/bg-20-1047-2023, https://doi.org/10.5194/bg-20-1047-2023, 2023
Short summary
Short summary
To improve the interpretation of the δ18Oatm and Δ17O of O2 in air bubbles in ice cores, we need to better quantify the oxygen fractionation coefficients associated with biological processes. We performed a simplified analogue of the terrestrial biosphere in a closed chamber. We found a respiration fractionation in agreement with the previous estimates at the microorganism scale, and a terrestrial photosynthetic fractionation was found. This has an impact on the estimation of the Dole effect.
Adam Francis, Raja S. Ganeshram, Robyn E. Tuerena, Robert G. M. Spencer, Robert M. Holmes, Jennifer A. Rogers, and Claire Mahaffey
Biogeosciences, 20, 365–382, https://doi.org/10.5194/bg-20-365-2023, https://doi.org/10.5194/bg-20-365-2023, 2023
Short summary
Short summary
Climate change is causing extensive permafrost degradation and nutrient releases into rivers with great ecological impacts on the Arctic Ocean. We focused on nitrogen (N) release from this degradation and associated cycling using N isotopes, an understudied area. Many N species are released at degradation sites with exchanges between species. N inputs from permafrost degradation and seasonal river N trends were identified using isotopes, helping to predict climate change impacts.
Mohamed Ayache, Jean-Claude Dutay, Kazuyo Tachikawa, Thomas Arsouze, and Catherine Jeandel
Biogeosciences, 20, 205–227, https://doi.org/10.5194/bg-20-205-2023, https://doi.org/10.5194/bg-20-205-2023, 2023
Short summary
Short summary
The neodymium (Nd) is one of the most useful tracers to fingerprint water mass provenance. However, the use of Nd is hampered by the lack of adequate quantification of the external sources. Here, we present the first simulation of dissolved Nd concentration and Nd isotopic composition in the Mediterranean Sea using a high-resolution model. We aim to better understand how the various external sources affect the Nd cycle and particularly assess how it is impacted by atmospheric inputs.
Marta Santos-Garcia, Raja S. Ganeshram, Robyn E. Tuerena, Margot C. F. Debyser, Katrine Husum, Philipp Assmy, and Haakon Hop
Biogeosciences, 19, 5973–6002, https://doi.org/10.5194/bg-19-5973-2022, https://doi.org/10.5194/bg-19-5973-2022, 2022
Short summary
Short summary
Terrestrial sources of nitrate are important contributors to the nutrient pool in the fjords of Kongsfjorden and Rijpfjorden in Svalbard during the summer, and they sustain most of the fjord primary productivity. Ongoing tidewater glacier retreat is postulated to favour light limitation and less dynamic circulation in fjords. This is suggested to encourage the export of nutrients to the middle and outer part of the fjord system, which may enhance primary production within and in offshore areas.
Marlene Dordoni, Michael Seewald, Karsten Rinke, Kurt Friese, Robert van Geldern, Jakob Schmidmeier, and Johannes A. C. Barth
Biogeosciences, 19, 5343–5355, https://doi.org/10.5194/bg-19-5343-2022, https://doi.org/10.5194/bg-19-5343-2022, 2022
Short summary
Short summary
Organic matter (OM) turnover into dissolved inorganic carbon (DIC) was investigated by means of carbon isotope mass balances in Germany's largest water reservoir. This includes a metalimnetic oxygen minimum (MOM). Autochthonous particulate organic carbon (POC) was the main contributor to DIC, with rates that were highest for the MOM. Generally low turnover rates outline the environmental fragility of this water body in the case that OM loads increase due to storm events or land use changes.
Frédérique M. S. A. Kirkels, Hugo J. de Boer, Paulina Concha Hernández, Chris R. T. Martes, Marcel T. J. van der Meer, Sayak Basu, Muhammed O. Usman, and Francien Peterse
Biogeosciences, 19, 4107–4127, https://doi.org/10.5194/bg-19-4107-2022, https://doi.org/10.5194/bg-19-4107-2022, 2022
Short summary
Short summary
The distinct carbon isotopic values of C3 and C4 plants are widely used to reconstruct past hydroclimate, where more C3 plants reflect wetter and C4 plants drier conditions. Here we examine the impact of regional hydroclimatic conditions on plant isotopic values in the Godavari River basin, India. We find that it is crucial to identify regional plant isotopic values and consider drought stress, which introduces a bias in C3 / C4 plant estimates and associated hydroclimate reconstructions.
Mhlangabezi Mdutyana, Tanya Marshall, Xin Sun, Jessica M. Burger, Sandy J. Thomalla, Bess B. Ward, and Sarah E. Fawcett
Biogeosciences, 19, 3425–3444, https://doi.org/10.5194/bg-19-3425-2022, https://doi.org/10.5194/bg-19-3425-2022, 2022
Short summary
Short summary
Nitrite-oxidizing bacteria in the winter Southern Ocean show a high affinity for nitrite but require a minimum (i.e., "threshold") concentration before they increase their rates of nitrite oxidation significantly. The classic Michaelis–Menten model thus cannot be used to derive the kinetic parameters, so a modified equation was employed that also yields the threshold nitrite concentration. Dissolved iron availability may play an important role in limiting nitrite oxidation.
Weitian Ding, Urumu Tsunogai, Fumiko Nakagawa, Takashi Sambuichi, Hiroyuki Sase, Masayuki Morohashi, and Hiroki Yotsuyanagi
Biogeosciences, 19, 3247–3261, https://doi.org/10.5194/bg-19-3247-2022, https://doi.org/10.5194/bg-19-3247-2022, 2022
Short summary
Short summary
Excessive leaching of nitrate from forested catchments during storm events degrades water quality and causes eutrophication in downstream areas. Thus, tracing the source of nitrate increase during storm events in forested streams is important for sustainable forest management. Based on the isotopic compositions of stream nitrate, including Δ17O, this study clarifies that the source of stream nitrate increase during storm events was soil nitrate in the riparian zone.
Nicolas Séon, Romain Amiot, Guillaume Suan, Christophe Lécuyer, François Fourel, Fabien Demaret, Arnauld Vinçon-Laugier, Sylvain Charbonnier, and Peggy Vincent
Biogeosciences, 19, 2671–2681, https://doi.org/10.5194/bg-19-2671-2022, https://doi.org/10.5194/bg-19-2671-2022, 2022
Short summary
Short summary
We analysed the oxygen isotope composition of bones and teeth of four marine species possessing regional heterothermies. We observed a consistent link between oxygen isotope composition and temperature heterogeneities recorded by classical methods. This opens up new perspectives on the determination of the thermoregulatory strategies of extant marine vertebrates where conventional methods are difficult to apply, but also allows us to investigate thermophysiologies of extinct vertebrates.
Yuwei Liu, Guofeng Zhu, Zhuanxia Zhang, Zhigang Sun, Leilei Yong, Liyuan Sang, Lei Wang, and Kailiang Zhao
Biogeosciences, 19, 877–889, https://doi.org/10.5194/bg-19-877-2022, https://doi.org/10.5194/bg-19-877-2022, 2022
Short summary
Short summary
We took the water cycle process of soil–plant–atmospheric precipitation as the research objective. In the water cycle of soil–plant–atmospheric precipitation, precipitation plays the main controlling role. The main source of replenishment for alpine meadow plants is precipitation and alpine meltwater; the main source of replenishment for forest plants is soil water; and the plants in the arid foothills mainly use groundwater.
Roberto Velázquez-Ochoa, María Julia Ochoa-Izaguirre, and Martín Federico Soto-Jiménez
Biogeosciences, 19, 1–27, https://doi.org/10.5194/bg-19-1-2022, https://doi.org/10.5194/bg-19-1-2022, 2022
Short summary
Short summary
Our research is the first approximation to understand the δ13C macroalgal variability in one of the most diverse marine ecosystems in the world, the Gulf of California. The life-form is the principal cause of δ13C macroalgal variability, mainly taxonomy. However, changes in habitat characteristics and environmental conditions also influence the δ13C macroalgal variability. The δ13C macroalgae is indicative of carbon concentration mechanisms and isotope discrimination during carbon assimilation.
Raquel F. Flynn, Thomas G. Bornman, Jessica M. Burger, Shantelle Smith, Kurt A. M. Spence, and Sarah E. Fawcett
Biogeosciences, 18, 6031–6059, https://doi.org/10.5194/bg-18-6031-2021, https://doi.org/10.5194/bg-18-6031-2021, 2021
Short summary
Short summary
Biological activity in the shallow Weddell Sea affects the biogeochemistry of recently formed deep waters. To investigate the drivers of carbon and nutrient export, we measured rates of primary production and nitrogen uptake, characterized the phytoplankton community, and estimated nutrient depletion ratios across the under-sampled western Weddell Sea in mid-summer. Carbon export was highest at the ice shelves and was determined by a combination of physical, chemical, and biological factors.
Stéphanie H. M. Jacquet, Christian Tamburini, Marc Garel, Aurélie Dufour, France Van Vambeke, Frédéric A. C. Le Moigne, Nagib Bhairy, and Sophie Guasco
Biogeosciences, 18, 5891–5902, https://doi.org/10.5194/bg-18-5891-2021, https://doi.org/10.5194/bg-18-5891-2021, 2021
Short summary
Short summary
We compared carbon remineralization rates (MRs) in the western and central Mediterranean Sea in late spring during the PEACETIME cruise, as assessed using the barium tracer. We reported higher and deeper (up to 1000 m depth) MRs in the western basin, potentially sustained by an additional particle export event driven by deep convection. The central basin is the site of a mosaic of blooming and non-blooming water masses and showed lower MRs that were restricted to the upper mesopelagic layer.
Shinsuke Kawagucci, Yohei Matsui, Akiko Makabe, Tatsuhiro Fukuba, Yuji Onishi, Takuro Nunoura, and Taichi Yokokawa
Biogeosciences, 18, 5351–5362, https://doi.org/10.5194/bg-18-5351-2021, https://doi.org/10.5194/bg-18-5351-2021, 2021
Short summary
Short summary
Hydrogen and carbon isotope ratios of methane as well as the relevant biogeochemical parameters and microbial community compositions in hydrothermal plumes in the Okinawa Trough were observed. We succeeded in simultaneously determining hydrogen and carbon isotope fractionation factors associated with aerobic oxidation of methane in seawater (εH = 49.4 ± 5.0 ‰, εC = 5.2 ± 0.4 ‰) – the former being the first of its kind ever reported.
Nicolai Schleinkofer, David Evans, Max Wisshak, Janina Vanessa Büscher, Jens Fiebig, André Freiwald, Sven Härter, Horst R. Marschall, Silke Voigt, and Jacek Raddatz
Biogeosciences, 18, 4733–4753, https://doi.org/10.5194/bg-18-4733-2021, https://doi.org/10.5194/bg-18-4733-2021, 2021
Short summary
Short summary
We have measured the chemical composition of the carbonate shells of the parasitic foraminifera Hyrrokkin sarcophaga in order to test if it is influenced by the host organism (bivalve or coral). We find that both the chemical and isotopic composition is influenced by the host organism. For example strontium is enriched in foraminifera that grew on corals, whose skeleton is built from aragonite, which is naturally enriched in strontium compared to the bivalves' calcite shell.
Inga Köhler, Raul E. Martinez, David Piatka, Achim J. Herrmann, Arianna Gallo, Michelle M. Gehringer, and Johannes A. C. Barth
Biogeosciences, 18, 4535–4548, https://doi.org/10.5194/bg-18-4535-2021, https://doi.org/10.5194/bg-18-4535-2021, 2021
Short summary
Short summary
We investigated how high Fe(II) levels influence the O2 budget of a circum-neutral Fe(II)-rich spring and if a combined study of dissolved O (DO) and its isotopic composition can help assess this effect. We showed that dissolved Fe(II) can exert strong effects on the δ18ODO even though a constant supply of atmospheric O2 occurs. In the presence of photosynthesis, direct effects of Fe oxidation become masked. Critical Fe(II) concentrations indirectly control the DO by enhancing photosynthesis.
Owen A. Sherwood, Samuel H. Davin, Nadine Lehmann, Carolyn Buchwald, Evan N. Edinger, Moritz F. Lehmann, and Markus Kienast
Biogeosciences, 18, 4491–4510, https://doi.org/10.5194/bg-18-4491-2021, https://doi.org/10.5194/bg-18-4491-2021, 2021
Short summary
Short summary
Pacific water flowing eastward through the Canadian Arctic plays an important role in redistributing nutrients to the northwest Atlantic Ocean. Using samples collected from northern Baffin Bay to the southern Labrador Shelf, we show that stable isotopic ratios in seawater nitrate reflect the fraction of Pacific to Atlantic water. These results provide a new framework for interpreting patterns of nitrogen isotopic variability recorded in modern and archival organic materials in the region.
Franziska Slotta, Lukas Wacker, Frank Riedel, Karl-Uwe Heußner, Kai Hartmann, and Gerhard Helle
Biogeosciences, 18, 3539–3564, https://doi.org/10.5194/bg-18-3539-2021, https://doi.org/10.5194/bg-18-3539-2021, 2021
Short summary
Short summary
The African baobab is a challenging climate and environmental archive for its semi-arid habitat due to dating uncertainties and parenchyma-rich wood anatomy. Annually resolved F14C data of tree-ring cellulose (1941–2005) from a tree in Oman show the annual character of the baobab’s growth rings but were up to 8.8 % lower than expected for 1964–1967. Subseasonal δ13C and δ18O patterns reveal years with low average monsoon rain as well as heavy rainfall events from pre-monsoonal cyclones.
Peter M. J. Douglas, Emerald Stratigopoulos, Sanga Park, and Dawson Phan
Biogeosciences, 18, 3505–3527, https://doi.org/10.5194/bg-18-3505-2021, https://doi.org/10.5194/bg-18-3505-2021, 2021
Short summary
Short summary
Hydrogen isotopes could be a useful tool to help resolve the geographic distribution of methane emissions from freshwater environments. We analyzed an expanded global dataset of freshwater methane hydrogen isotope ratios and found significant geographic variation linked to water isotopic composition. This geographic variability could be used to resolve changing methane fluxes from freshwater environments and provide more accurate estimates of the relative balance of global methane sources.
Veronica R. Rollinson, Julie Granger, Sydney C. Clark, Mackenzie L. Blanusa, Claudia P. Koerting, Jamie M. P. Vaudrey, Lija A. Treibergs, Holly C. Westbrook, Catherine M. Matassa, Meredith G. Hastings, and Craig R. Tobias
Biogeosciences, 18, 3421–3444, https://doi.org/10.5194/bg-18-3421-2021, https://doi.org/10.5194/bg-18-3421-2021, 2021
Short summary
Short summary
We measured nutrients and the naturally occurring nitrogen (N) and oxygen (O) stable isotope ratios of nitrate discharged from a New England river over an annual cycle, to monitor N loading and identify dominant sources from the watershed. We uncovered a seasonality to loading and sources of N from the watershed. Seasonality in the nitrate isotope ratios also informed on N cycling, conforming to theoretical expectations of riverine nutrient cycling.
Zixun Chen, Xuejun Liu, Xiaoqing Cui, Yaowen Han, Guoan Wang, and Jiazhu Li
Biogeosciences, 18, 2859–2870, https://doi.org/10.5194/bg-18-2859-2021, https://doi.org/10.5194/bg-18-2859-2021, 2021
Short summary
Short summary
δ13C in plants is a sensitive long-term indicator of physiological acclimatization. The present study suggests that precipitation change and increasing atmospheric N deposition have little impact on δ13C of H. ammodendron, a dominant plant in central Asian deserts, but affect its gas exchange. In addition, this study shows that δ13C of H. ammodendron could not indicate its water use efficiency (WUE), suggesting that whether δ13C of C4 plants indicates WUE is species-specific.
Petra Zahajská, Carolina Olid, Johanna Stadmark, Sherilyn C. Fritz, Sophie Opfergelt, and Daniel J. Conley
Biogeosciences, 18, 2325–2345, https://doi.org/10.5194/bg-18-2325-2021, https://doi.org/10.5194/bg-18-2325-2021, 2021
Short summary
Short summary
The drivers of high accumulation of single-cell siliceous algae (diatoms) in a high-latitude lake have not been fully characterized before. We studied silicon cycling of the lake through water, radon, silicon, and stable silicon isotope balances. Results showed that groundwater brings 3 times more water and dissolved silica than the stream inlet. We demonstrate that groundwater discharge and low sediment deposition have driven the high diatom accumulation in the studied lake in the past century.
Yu-Te Hsieh, Walter Geibert, E. Malcolm S. Woodward, Neil J. Wyatt, Maeve C. Lohan, Eric P. Achterberg, and Gideon M. Henderson
Biogeosciences, 18, 1645–1671, https://doi.org/10.5194/bg-18-1645-2021, https://doi.org/10.5194/bg-18-1645-2021, 2021
Short summary
Short summary
The South Atlantic near 40° S is one of the high-productivity and most dynamic nutrient regions in the oceans, but the sources and fluxes of trace elements (TEs) to this region remain unclear. This study investigates seawater Ra-228 and provides important constraints on ocean mixing and dissolved TE fluxes to this region. Vertical mixing is a more important source than aeolian or shelf inputs in this region, but particulate or winter deep-mixing inputs may be required to balance the TE budgets.
Zhongjie Yu and Emily M. Elliott
Biogeosciences, 18, 805–829, https://doi.org/10.5194/bg-18-805-2021, https://doi.org/10.5194/bg-18-805-2021, 2021
Short summary
Short summary
In this study, we demonstrated distinct nitrogen isotope effects for nitric oxide (NO) production from major microbial and chemical NO sources in an agricultural soil. These results highlight characteristic bond-forming and breaking mechanisms associated with microbial and chemical NO production and implicate that simultaneous isotopic analyses of NO and nitrous oxide (N2O) can lead to unprecedented insights into the sources and processes controlling NO and N2O emissions from agricultural soils.
Daniel A. Frick, Rainer Remus, Michael Sommer, Jürgen Augustin, Danuta Kaczorek, and Friedhelm von Blanckenburg
Biogeosciences, 17, 6475–6490, https://doi.org/10.5194/bg-17-6475-2020, https://doi.org/10.5194/bg-17-6475-2020, 2020
Short summary
Short summary
Silicon is taken up by some plants to increase structural stability and to develop stress resistance and is rejected by others. To explore the underlying mechanisms, we used the stable isotopes of silicon that shift in their relative abundance depending on the biochemical transformation involved. On species with a rejective (tomato, mustard) and active (wheat) uptake mechanism, grown in hydroculture, we found that the transport of silicic acid is controlled by the precipitation of biogenic opal.
Quentin Charbonnier, Julien Bouchez, Jérôme Gaillardet, and Éric Gayer
Biogeosciences, 17, 5989–6015, https://doi.org/10.5194/bg-17-5989-2020, https://doi.org/10.5194/bg-17-5989-2020, 2020
Short summary
Short summary
The abundance and isotope composition of the trace metal barium (Ba) allows us to track and quantify nutrient cycling throughout the Amazon Basin. In particular, we show that the Ba biological fingerprint evolves from that of a strong net nutrient uptake in the mountainous area of the Andes towards efficient nutrient recycling on the plains of the Lower Amazon. Our study highlights the fact that the geochemical signature of rock-derived nutrients transported by the Amazon is scarred by life.
Ajinkya G. Deshpande, Thomas W. Boutton, Ayumi Hyodo, Charles W. Lafon, and Georgianne W. Moore
Biogeosciences, 17, 5639–5653, https://doi.org/10.5194/bg-17-5639-2020, https://doi.org/10.5194/bg-17-5639-2020, 2020
Short summary
Short summary
Wetland forests in the southern USA are threatened by changing climate and human-induced pressures. We used tree ring widths and C isotopes as indicators of forest growth and physiological stress, respectively, and compared these to past climate data. We observed that vegetation growing in the drier patches is susceptible to stress, while vegetation growth and physiology in wetter patches is less sensitive to unfavorable environmental conditions, highlighting the importance of optimal wetness.
Dominika Lewicka-Szczebak, Maciej Piotr Lewicki, and Reinhard Well
Biogeosciences, 17, 5513–5537, https://doi.org/10.5194/bg-17-5513-2020, https://doi.org/10.5194/bg-17-5513-2020, 2020
Short summary
Short summary
We present the first validation of N2O isotopic approaches for estimating N2O source pathways and N2O reduction. These approaches are widely used for tracing soil nitrogen cycling, but the results of these estimations are very uncertain. Here we report the results from parallel treatments allowing for precise validation of these approaches, and we propose the best strategies for results interpretation, including the new idea of an isotope model integrating three isotopic signatures of N2O.
Markus Raitzsch, Claire Rollion-Bard, Ingo Horn, Grit Steinhoefel, Albert Benthien, Klaus-Uwe Richter, Matthieu Buisson, Pascale Louvat, and Jelle Bijma
Biogeosciences, 17, 5365–5375, https://doi.org/10.5194/bg-17-5365-2020, https://doi.org/10.5194/bg-17-5365-2020, 2020
Short summary
Short summary
The isotopic composition of boron in carbonate shells of marine unicellular organisms is a popular tool to estimate seawater pH. Usually, many shells need to be dissolved and measured for boron isotopes, but the information on their spatial distribution is lost. Here, we investigate two techniques that allow for measuring boron isotopes within single shells and show that they yield robust mean values but provide additional information on the heterogeneity within and between single shells.
Florian Einsiedl, Anja Wunderlich, Mathieu Sebilo, Ömer K. Coskun, William D. Orsi, and Bernhard Mayer
Biogeosciences, 17, 5149–5161, https://doi.org/10.5194/bg-17-5149-2020, https://doi.org/10.5194/bg-17-5149-2020, 2020
Short summary
Short summary
Nitrate pollution of freshwaters and methane emissions into the atmosphere are crucial factors in deteriorating the quality of drinking water and in contributing to global climate change. Here, we report vertical concentration and stable isotope profiles of CH4, NO3-, NO2-, and NH4+ in the water column of Fohnsee (southern Bavaria, Germany) that may indicate linkages between nitrate-dependent anaerobic methane oxidation and the anaerobic oxidation of ammonium.
Ruifang C. Xie, Frédéric A. C. Le Moigne, Insa Rapp, Jan Lüdke, Beat Gasser, Marcus Dengler, Volker Liebetrau, and Eric P. Achterberg
Biogeosciences, 17, 4919–4936, https://doi.org/10.5194/bg-17-4919-2020, https://doi.org/10.5194/bg-17-4919-2020, 2020
Short summary
Short summary
Thorium-234 (234Th) is widely used to study carbon fluxes from the surface ocean to depth. But few studies stress the relevance of oceanic advection and diffusion on the downward 234Th fluxes in nearshore environments. Our study in offshore Peru showed strong temporal variations in both the importance of physical processes on 234Th flux estimates and the oceanic residence time of 234Th, whereas salinity-derived seawater 238U activities accounted for up to 40 % errors in 234Th flux estimates.
Ralf A. Oeser and Friedhelm von Blanckenburg
Biogeosciences, 17, 4883–4917, https://doi.org/10.5194/bg-17-4883-2020, https://doi.org/10.5194/bg-17-4883-2020, 2020
Short summary
Short summary
We present a novel strategy to decipher the relative impact of biogenic and abiotic drivers of weathering. We parameterized the nutrient fluxes in four ecosystems along a climate and vegetation gradient situated on the Chilean Coastal Cordillera. We investigated how nutrient demand by plants drives weathering. We found that the increase in biomass nutrient demand is accommodated by faster nutrient recycling rather than an increase in the weathering–release rates.
Tito Arosio, Malin M. Ziehmer, Kurt Nicolussi, Christian Schlüchter, and Markus Leuenberger
Biogeosciences, 17, 4871–4882, https://doi.org/10.5194/bg-17-4871-2020, https://doi.org/10.5194/bg-17-4871-2020, 2020
Short summary
Short summary
Stable isotopes in tree-ring cellulose are tools for climatic reconstructions, but interpretation is challenging due to nonclimate trends. We analyzed the tree-age trends in tree-ring isotopes of deciduous larch and evergreen cembran pine. Samples covering the whole Holocene were collected at the tree line in the Alps. For cambial ages over 100 years, we prove the absence of age trends in δD, δ18O, and δ13C for both species. For lower cambial ages, trends differ for each isotope and species.
Yuyang He, Xiaobin Cao, and Huiming Bao
Biogeosciences, 17, 4785–4795, https://doi.org/10.5194/bg-17-4785-2020, https://doi.org/10.5194/bg-17-4785-2020, 2020
Short summary
Short summary
Different carbon sites in a large organic molecule have different isotope compositions. Different carbon sites may not have the chance to exchange isotopes at all. The lack of appreciation of this notion might be blamed for an unsettled debate on the thermodynamic state of an organism. Here we demonstrate using minerals, N2O, and acetic acid that the dearth of exchange among different carbon sites renders them as independent as if they were different elements in organic molecules.
Felix M. Spielmann, Albin Hammerle, Florian Kitz, Katharina Gerdel, and Georg Wohlfahrt
Biogeosciences, 17, 4281–4295, https://doi.org/10.5194/bg-17-4281-2020, https://doi.org/10.5194/bg-17-4281-2020, 2020
Short summary
Short summary
Carbonyl sulfide (COS) can be used as a proxy for plant photosynthesis on an ecosystem scale. However, the relationships between COS and CO2 fluxes and their dependence on daily to seasonal changes in environmental drivers are still poorly understood. We examined COS and CO2 ecosystem fluxes above an agriculturally used mountain grassland for 6 months. Harvesting of the grassland disturbed the otherwise stable COS-to-CO2 uptake ratio. We even found the canopy to release COS during those times.
Getachew Agmuas Adnew, Thijs L. Pons, Gerbrand Koren, Wouter Peters, and Thomas Röckmann
Biogeosciences, 17, 3903–3922, https://doi.org/10.5194/bg-17-3903-2020, https://doi.org/10.5194/bg-17-3903-2020, 2020
Short summary
Short summary
We measured the effect of photosynthesis, the largest flux in the carbon cycle, on the triple oxygen isotope composition of atmospheric CO2 at the leaf level during gas exchange using three plant species. The main factors that limit the impact of land vegetation on the triple oxygen isotope composition of atmospheric CO2 are identified, characterized and discussed. The effect of photosynthesis on the isotopic composition of CO2 is commonly quantified as discrimination (ΔA).
Moritz Schroll, Frank Keppler, Markus Greule, Christian Eckhardt, Holger Zorn, and Katharina Lenhart
Biogeosciences, 17, 3891–3901, https://doi.org/10.5194/bg-17-3891-2020, https://doi.org/10.5194/bg-17-3891-2020, 2020
Short summary
Short summary
Fungi have recently been identified to produce the greenhouse gas methane. Here, we investigated the stable carbon isotope values of methane produced by saprotrophic fungi. Our results show that stable isotope values of methane from fungi are dependent on the fungal species and the metabolized substrate. They cover a broad range and overlap with stable carbon isotope values of methane reported for methanogenic archaea, the thermogenic degradation of organic matter, and other eukaryotes.
Pranav Hirave, Guido L. B. Wiesenberg, Axel Birkholz, and Christine Alewell
Biogeosciences, 17, 2169–2180, https://doi.org/10.5194/bg-17-2169-2020, https://doi.org/10.5194/bg-17-2169-2020, 2020
Short summary
Short summary
Sediment input into water bodies is a prominent threat to freshwater ecosystems. We tested the stability of tracers employed in freshwater sediment tracing based on compound-specific isotope analysis during early degradation in soil. While bulk δ13C values showed no stability, δ13C values of plant-derived fatty acids and n-alkanes were stably transferred to the soil without soil particle size dependency after an early degradation in organic horizons, thus indicating their suitability as tracers.
Caroline Thaler, Amandine Katz, Magali Bonifacie, Bénédicte Ménez, and Magali Ader
Biogeosciences, 17, 1731–1744, https://doi.org/10.5194/bg-17-1731-2020, https://doi.org/10.5194/bg-17-1731-2020, 2020
Short summary
Short summary
Paleoenvironment reconstructions, retrieved from δ18O and Δ47 values measured in carbonate, are compromised when crystallization occurs in isotopic disequilibrium. We show that some paleoenvironmental information can still be retrieved from these paired disequilibrium Δ47 and δ18O values. The possibility of retrieving information on paleowaters, sediments' interstitial waters, or organisms' body water at the carbonate precipitation loci will help understand past Earth and life evolution.
Guillaume Humbert, Mathieu Sébilo, Justine Fiat, Longqi Lang, Ahlem Filali, Véronique Vaury, Mathieu Spérandio, and Anniet M. Laverman
Biogeosciences, 17, 979–993, https://doi.org/10.5194/bg-17-979-2020, https://doi.org/10.5194/bg-17-979-2020, 2020
Short summary
Short summary
Mitigating emissions of the greenhouse gas N2O requires understanding of the relative contribution of its producing processes in response to environmental variables. We show, using isotopic analysis, that N2O emissions from a nitrifying system were sensitive to oxygenation, temperature and NH4+ concentrations with nitrite reduction being the main N2O source. Temperature appears to be the main control on N2O production, due to its dissimilar effects on ammonium and nitrite oxidizing activities.
Oguz Yigiterhan, Ebrahim Mohd Al-Ansari, Alex Nelson, Mohamed Alaa Abdel-Moati, Jesse Turner, Hamood Abdulla Alsaadi, Barbara Paul, Ibrahim Abdullatif Al-Maslamani, Mehsin Abdulla Al-Ansi Al-Yafei, and James W. Murray
Biogeosciences, 17, 381–404, https://doi.org/10.5194/bg-17-381-2020, https://doi.org/10.5194/bg-17-381-2020, 2020
Short summary
Short summary
We analyze net-tow samples of plankton and associated particulate matter from the Exclusive Economic Zone, Qatar, Arabian Gulf, using net tows with mesh sizes of 50 and 200 μm to examine the composition of plankton populations. We also focus on the role and composition of the atmospheric dust, representative of terrigenous material, deposited in the Gulf. We concluded that Al, Fe, Cr, Co, Mn, Ni, Pb, and Li are of dust origin and As, Cd, Cu, Mo, Zn, and Ca are of anthropogenic/biogenic origin.
Cited articles
Aerssens, E., Tiedje, J. M., and Averill, B. A.:
Isotope labeling studies on the mechanisms of N-bond formation in denitrification,
J. Biol. Chem.,
261, 9652–9656, 1986.
Anderson, J. P. E. and Domsch, K. H.:
Quantification of bacterial and fungal contributions to soil respiration,
Arch Mikrobiol.,
93, 113–127, https://doi.org/10.1007/BF00424942, 1973.
Anderson, J. P. E. and Domsch, K. H.:
Measurement of bacterial and fungal contributions to respiration of selected agricultural and forest soil,
Can. J. Microbiol.,
21, 314–322, https://doi.org/10.1139/m75-045, 1975.
Anderson, J. P. E. and Domsch, K. H.:
Physiological method for quantitative measurement of microbial biomass in soils,
Soil Biol. Biochem.,
10, 215–221, https://doi.org/10.1016/0038-0717(78)90099-8, 1978.
Bergsma, T. T., Ostrom, N. E., Emmons, M., and Robertson, G. P.:
Measuring simultaneous fluxes from soil of N2O and N2 in the field using the 15N-Gas “nonequilihrium” technique,
Environ. Sci. Technol.,
35, 4307–4312, https://doi.org/10.1021/es010885u, 2001.
Blagodatskaya, E. V. and Anderson, T.-H.:
Interactive effects of pH and substrate quality on the fungal-to-bacterial ratio and qCO2 of microbial communities in forest soils,
Soil Biol. Biochem.,
30, 1269–1274, https://doi.org/10.1016/S0038-0717(98)00050-9, 1998.
Blagodatskaya, E., Dannenmann, M., Gasche, R., and Butterbach-Bahl, K.:
Microclimate and forest management alter fungal-to-bacterial ratio and N2O-emission during rewetting in the forest floor and mineral soil of mountainous beech forests,
Biogeochemistry,
97, 55–70, https://doi.org/10.1007/s10533-009-9310-3, 2010.
Bollag, J. M. and Tung, G.:
Nitrous oxide release by soil fungi,
Soil Biol. Biochem.,
4, 271–276, https://doi.org/10.1016/0038-0717(72)90021-1, 1972.
Bollmann, A. and Conrad, R.:
Enhancement by acetylene of the decomposition of nitric oxide in soil,
Soil Biol. Biochem.,
29, 1057–1066, 1997a.
Bollmann, A. and Conrad, R.:
Acetylene blockage technique leads to underestimation of denitrification rates in oxic soils due to scavenging of intermediate nitric oxide,
Soil Biol. Biochem.,
29, 1067–1077, 1997b.
Braker, G. and Conrad, R.:
Diversity, structure, and size of N2O-producing microbial communities in soils–what matters for their functioning?,
Adv. Appl. Microbiol., 33–70, 2011.
Brand, W. A.:
Precon: A fully automated interface for the pre-GC concentration of trace gases in air for isotopic analysis,
Isot. Environ. Healt. S.,
31, 277–284, https://doi.org/10.1080/10256019508036271, 1995.
Bremner, J.:
Sources of nitrous oxide in soils,
Nutr. Cycl. Agroecosys.,
49, 7–16, https://doi.org/10.1023/A:1009798022569, 1997.
Buchen, C., Lewicka-Szczebak, D., Flessa, H., and Well, R.:
Estimating N2O processes during grassland renewal and grassland conversion to maize cropping using N2O isotopocules,
Rapid Commun. Mass Sp.,
32, 1053–1067, https://doi.org/10.1002/rcm.8132, 2018.
Casciotti, K. L., Sigman, D. M., Hastings, M. G., Böhlke, J. K., and Hilkert, A.:
Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method,
Anal. Chem.,
74, 4905–4912, https://doi.org/10.1021/ac020113w, 2002.
Chen, H., Mothapo, N. V., and Shi, W.:
The significant contribution of fungi to soil N2O production across diverse ecosystems,
Appl. Soil Ecol.,
73, 70–77, https://doi.org/10.1016/j.apsoil.2013.08.011, 2014.
Chen, H. H., Mothapo, N. V., and Shi, W.:
Soil Moisture and pH Control Relative Contributions of Fungi and Bacteria to N2O Production,
Microb. Ecol.,
69, 180–191, https://doi.org/10.1007/s00248-014-0488-0, 2015.
Clough, T. J., Jarvis, S. C., Dixon, E. R., Stevens, R. J., Laughlin, R. J., and Hatch, D. J.:
Carbon induced subsoil denitrification of 15N-labelled nitrate in 1 m deep soil columns,
Soil Biol. Biochem.,
31, 31–41, https://doi.org/10.1016/S0038-0717(98)00097-2, 1998.
Crenshaw, C. L., Lauber, C., Sinsabaugh, R. L., and Stavely, L. K.:
Fungal control of nitrous oxide production in semiarid grassland,
Biogeochemistry,
87, 17–27, https://doi.org/10.1007/s10533-007-9165-4, 2008.
Crutzen, P. J.:
The influence of nitrogen oxides on the atmospheric ozone content,
Q. J. Roy. Meteor. Soc.,
96, 320–325, https://doi.org/10.1002/qj.49709640815, 1970.
Decock, C. and Six, J.:
How reliable is the intramolecular distribution of 15N in N2O to source partition N2O emitted from soil?,
Soil Biol. Biochem.,
65, 114–127, https://doi.org/10.1016/j.soilbio.2013.05.012, 2013.
Firestone, M. K. and Davidson, E. A.: Microbiological Basis of NO and N2O Production and Consumption in Soils, in: Exchange of Trace Gases between Terrestrial Ecosystems and the Atmosphere, edited by: Andreae, M. O. and Schimel, D. S., John Willey and Sons, New York, 7–21, 1989.
Frame, C. H. and Casciotti, K. L.: Biogeochemical controls and isotopic signatures of nitrous oxide production by a marine ammonia-oxidizing bacterium, Biogeosciences, 7, 2695–2709, https://doi.org/10.5194/bg-7-2695-2010, 2010.
Garber, E. A. and Hollocher, T. C.:
15N, 18O tracer studies on the activation of nitrite by denitrifying bacteria. Nitrite/water-oxygen exchange and nitrosation reactions as indicators of electrophilic catalysis,
J. Biol. Chem.,
257, 8091–8097, https://doi.org/10.1007/BF00399539, 1982.
Groffman, P. M., Altabet, M. A., Bohlke, J. K., Butterbach-Bahl, K., David, M. B., Firestone, M. K., Giblin, A. E., Kana, T. M., Nielsen, L. P., and Voytek, M. A.:
Methods for measuring denitrification: Diverse approaches to a difficult problem,
Ecol. Appl.,
16, 2091–2122, 2006.
Hayatsu, M., Tago, K., and Saito, M.:
Various players in the nitrogen cycle: Diversity and functions of the microorganisms involved in nitrification and denitrification,
Soil Sci. Plant Nutr.,
54, 33–45, https://doi.org/10.1111/j.1747-0765.2007.00195.x, 2008.
Higgins, S. A., Schadt, C. W., Matheny, P. B., and Löffler, F. E.:
Phylogenomics reveal the dynamic evolution of fungal nitric oxide reductases and their relationship to secondary metabolism,
Genome Biol. Evol.,
10, 2474–2489, https://doi.org/10.1093/gbe/evy187, 2018.
IPCC:
Climate Change 2013: The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change,
Cambridge, UK and New York, NY, USA, 1535 pp., 2013.
Jinuntuya-Nortman, M., Sutka, R. L., Ostrom, P. H., Gandhi, H., and Ostrom, N. E.:
Isotopologue fractionation during microbial reduction of N2O within soil mesocosms as a function of water-filled pore space,
Soil Biol. Biochem.,
40, 2273–2280, https://doi.org/10.1016/j.soilbio.2008.05.016, 2008.
Keuschnig, C., Gorfer, M., Li, G., Mania, D., Frostegård, Å., Bakken, L., and Larose, C.:
NO and N2O transformations of diverse fungi in hypoxia: evidence for anaerobic respiration only in Fusarium strains,
Environ. Microbiol.,
22, 2182–2195, https://doi.org/10.1111/1462-2920.14980, 2020.
Knowles, R.:
Denitrification,
Microbiol. Rev.,
46, 43–70, 1982.
Kool, D. M., Wrage, N., Oenema, O., Dolfing, J., and Van Groenigen, J. W.:
Oxygen exchange between (de)nitrification intermediates and H2O and its implications for source determination of
Kool, D. M., Wrage, N., Oenema, O., Harris, D., and Van Groenigen, J. W.:
The 18O signature of biogenic nitrous oxide is determined by O exchange with water,
Rapid Commun. Mass Sp.,
23, 104–108, https://doi.org/10.1002/rcm.3859, 2009.
Koranda, M., Kaiser, C., Fuchslueger, L., Kitzler, B., Sessitsch, A., Zechmeister-Boltenstern, S., and Richter, A.:
Fungal and bacterial utilization of organic substrates depends on substrate complexity and N availability,
FEMS Microbiol. Ecol.,
87, 142–152, https://doi.org/10.1111/1574-6941.12214, 2014.
Köster, J. R., Well, R., Dittert, K., Giesemann, A., Lewicka-Szczebak, D., Muehling, K.-H., Herrmann, A., Lammel, J., and Senbayram, M.:
Soil denitrification potential and its influence on N2O reduction and N2O isotopomer ratios,
Rapid Commun. Mass Sp.,
27, 2363–2373, https://doi.org/10.1002/rcm.6699, 2013a.
Köster, J. R., Well, R., Tuzson, B., Bol, R., Dittert, K., Giesemann, A., Emmenegger, L., Manninen, A., Cárdenas, L., and Mohn, J.:
Novel laser spectroscopic technique for continuous analysis of N2O isotopomers – application and intercomparison with isotope ratio mass spectrometry,
Rapid Commun. Mass Sp.,
27, 216–222, https://doi.org/10.1002/rcm.6434, 2013b.
Ladan, S. and Jacinthe, P.-A.:
Evaluation of antibacterial and antifungal compounds for selective inhibition of denitrification in soils,
Environ. Sci.-Proc. Imp.,
18, 1519–1529, https://doi.org/10.1039/C6EM00456C, 2016.
Laughlin, R. J. and Stevens, R. J.:
Evidence for fungal dominance of denitrification and codenitrification in a grassland soil,
Soil Sci. Soc. Am. J.,
66, 1540–1548, https://doi.org/10.2136/sssaj2002.1540, 2002.
Lewicka-Szczebak, D., Well, R., Giesemann, A., Rohe, L., and Wolf, U.:
An enhanced technique for automated determination of 15N signatures of N2, (N2+N2O) and N2O in gas samples,
Rapid Commun. Mass Sp.,
27, 1548–1558, https://doi.org/10.1002/rcm.6605, 2013.
Lewicka-Szczebak, D., Well, R., Köster, J. R., Fuß, R., Senbayram, M., Dittert, K., and Flessa, H.:
Experimental determinations of isotopic fractionation factors associated with N2O production and reduction during denitrification in soils,
Geochim. Cosmochim. Ac.,
134, 55–73, https://doi.org/10.1016/j.gca.2014.03.010, 2014.
Lewicka-Szczebak, D., Well, R., Bol, R., Gregory, A. S., Matthews, G. P., Misselbrook, T., Whalley, W. R., and Cardenas, L. M.:
Isotope fractionation factors controlling isotopocule signatures of soil-emitted N2O produced by denitrification processes of various rates,
Rapid Commun. Mass Sp.,
29, 269–282, https://doi.org/10.1002/rcm.7102, 2015.
Lewicka-Szczebak, D., Dyckmans, J., Kaiser, J., Marca, A., Augustin, J., and Well, R.: Oxygen isotope fractionation during N2O production by soil denitrification, Biogeosciences, 13, 1129–1144, https://doi.org/10.5194/bg-13-1129-2016, 2016.
Lewicka-Szczebak, D., Augustin, J., Giesemann, A., and Well, R.:
Quantifying N2O reduction to N2 based on N2O isotopocules – validation with independent methods (helium incubation and 15N gas flux method), Biogeosciences, 14, 711–732, https://doi.org/10.5194/bg-14-711-2017, 2017.
Lewicka-Szczebak, D., Lewicki, M. P., and Well, R.: N2O isotope approaches for source partitioning of N2O production and estimation of N2O reduction – validation with the 15N gas-flux method in laboratory and field studies, Biogeosciences, 17, 5513–5537, https://doi.org/10.5194/bg-17-5513-2020, 2020.
Long, A., Heitman, J., Tobias, C., Philips, R., and Song, B.:
Co-occurring anammox, denitrification, and codenitrification in agricultural soils, Applied and
Environ. Microbiol.,
79, 168–176, https://doi.org/10.1128/aem.02520-12, 2013.
Maeda, K., Spor, A., Edel-Hermann, V., Heraud, C., Breuil, M.-C., Bizouard, F., Toyoda, S., Yoshida, N., Steinberg, C., and Philippot, L.:
N2O production, a widespread trait in fungi,
Sci. Rep.-UK,
5, 9697, https://doi.org/10.1038/srep09697, 2015.
McLain, J. E. T. and Martens, D. A.:
N2O production by heterotrophic N transformations in a semiarid soil,
Appl. Soil Ecol.,
32, 253–263, 2006.
Müller, C. and Clough, T. J.:
Advances in understanding nitrogen flows and transformations: gaps and research pathways,
J. Agr. Sci.,
152, S34-S44, https://doi.org/10.1017/s0021859613000610, 2014.
Nadeem, S., Dorsch, P., and Bakken, L. R.:
Autoxidation and acetylene-accelerated oxidation of NO in a 2-phase system: Implications for the expression of denitrification in ex situ experiments,
Soil Biol. Biochem.,
57, 606–614, https://doi.org/10.1016/j.soilbio.2012.10.007, 2013.
Ostrom, N. and Ostrom, P.:
The Isotopomers of Nitrous Oxide: Analytical Considerations and Application to Resolution of Microbial Production Pathways,
in: Handbook of Environmental Isotope Geochemistry,
edited by: Baskaran, M.,
Advances in Isotope Geochemistry,
Springer Berlin Heidelberg, 453–476, 2011.
Ostrom, N. E. and Ostrom, P. H.:
Mining the isotopic complexity of nitrous oxide: a review of challenges and opportunities,
Biogeochemistry,
132, 359–372, https://doi.org/10.1007/s10533-017-0301-5, 2017.
Ostrom, N. E., Pitt, A., Sutka, R., Ostrom, P. H., Grandy, A. S., Huizinga, K. M., and Robertson, G. P.:
Isotopologue effects during N2O reduction in soils and in pure cultures of denitrifiers,
J. Geophys. Res.-Biogeo.,
112, G02005, https://doi.org/10.1029/2006jg000287, 2007.
Ostrom, N. E., Sutka, R., Ostrom, P. H., Grandy, A. S., Huizinga, K. M., Gandhi, H., von Fischer, J. C., and Robertson, G. P.:
Isotopologue data reveal bacterial denitrification as the primary source of N2O during a high flux event following cultivation of a native temperate grassland,
Soil Biol. Biochem.,
42, 499–506, https://doi.org/10.1016/j.soilbio.2009.12.003, 2010.
Pan, Y. S., Wu, Y. C., Li, X. Z., Zeng, J., and Lin, X. G.:
Continuing Impacts of Selective Inhibition on Bacterial and Fungal Communities in an Agricultural Soil,
Microb. Ecol.,
78, 927–935, https://doi.org/10.1007/s00248-019-01364-0, 2019.
Philippot, L., Hallin, S., and Schloter, M.: Ecology of denitrifying prokaryotes in agricultural soil, Adv. Agron., 96, 249–305, 2007.
Popp, B. N., Westley, M. B., Toyoda, S., Miwa, T., Dore, J. E., Yoshida, N., Rust, T. M., Sansone, F. J., Russ, M. E., Ostrom, N. E., and Ostrom, P. H.:
Nitrogen and oxygen isotopomeric constraints on the origins and sea-to-air flux of N2O in the oligotrophic subtropical North Pacific gyre,
Global Biogeochem. Cy.,
16, 1064, https://doi.org/10.1029/2001gb001806, 2002.
R Core Team: R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing, Vienna, available at: https://www.R-project.org/ (last access: 11 May 2021), 2013.
Reischke, S., Rousk, J., and Bååth, E.:
The effects of glucose loading rates on bacterial and fungal growth in soil,
Soil Biol. Biochem.,
70, 88–95, https://doi.org/10.1016/j.soilbio.2013.12.011, 2014.
Rohe, L., Anderson, T.-H., Braker, G., Flessa, H., Giesemann, A., Lewicka-Szczebak, D., Wrage-Mönnig, N., and Well, R.:
Dual isotope and isotopomer signatures of nitrous oxide from fungal denitrification – a pure culture study,
Rapid Commun. Mass Sp.,
28, 1893–1903, https://doi.org/10.1002/rcm.6975, 2014a.
Rohe, L., Anderson, T.-H., Braker, G., Flessa, H., Giesemann, A., Wrage-Mönnig, N., and Well, R.:
Fungal oxygen exchange between denitrification intermediates and water,
Rapid Commun. Mass Sp.,
28, 377–384, https://doi.org/10.1002/rcm.6790, 2014b.
Rohe, L., Well, R., and Lewicka-Szczebak, D.:
Use of oxygen isotopes to differentiate between nitrous oxide produced by fungi or bacteria during denitrification,
Rapid Commun. Mass Sp.,
31, 1297–1312, https://doi.org/10.1002/rcm.7909, 2017.
Ruzicka, S., Edgerton, D., Norman, M., and Hill, T.:
The utility of ergosterol as a bioindicator of fungi in temperate soils,
Soil Biol. Biochem.,
32, 989–1005, https://doi.org/10.1016/S0038-0717(00)00009-2, 2000.
Senbayram, M., Well, R., Bol, R., Chadwick, D. R., Jones, D. L., and Wu, D.:
Interaction of straw amendment and soil
Senbayram, M., Well, R., Shan, J., Bol, R., Burkart, S., Jones, D. L., and Wu, D.:
Rhizosphere processes in nitrate-rich barley soil tripled both N2O and N2 losses due to enhanced bacterial and fungal denitrification,
Plant Soil,
448, 509–522, https://doi.org/10.1007/s11104-020-04457-9, 2020.
Seo, D. C. and DeLaune, R. D.:
Fungal and bacterial mediated denitrification in wetlands: Influence of sediment redox condition,
Water Res.,
44, 2441–2450, https://doi.org/10.1016/j.watres.2010.01.006, 2010.
Shoun, H., Kim, D.-H., Uchiyama, H., and Sugiyama, J.:
Denitrification by fungi,
FEMS Microbiol. Lett.,
94, 277–281, 1992.
Shoun, H., Fushinobu, S., Jiang, L., Kim, S. W., and Wakagi, T.: Fungal denitrification and nitric oxide reductase cytochrome P450nor, Philos. Trans. R. Soc. Lond. B. Biol. Sci., 367, 1186–1194, https://doi.org/10.1098/rstb.2011.0335, 2012.
Snider, D. M., Schiff, S. L., and Spoelstra, J.:
Spott, O., Russow, R., Apelt, B., and Stange, C. F.:
A 15N-aided artificial atmosphere gas flow technique for online determination of soil N2 release using the zeolite Köstrolith SX6®,
Rapid Commun. Mass Sp.,
20, 3267–3274, https://doi.org/10.1002/rcm.2722, 2006.
Spott, O., Russow, R., and Stange, C. F.:
Formation of hybrid N2O and hybrid N2 due to codenitrification: First review of a barely considered process of microbially mediated N-nitrosation,
Soil Biol. Biochem.,
43, 1995–2011, https://doi.org/10.1016/j.soilbio.2011.06.014, 2011.
Stumpp, C., Klaus, J., and Stichler, W.:
Analysis of long-term stable isotopic composition in German precipitation,
J. Hydrol.,
517, 351–361, https://doi.org/10.1016/j.jhydrol.2014.05.034, 2014.
Sutka, R. L., Ostrom, N. E., Ostrom, P. H., Breznak, J. A., Gandhi, H., Pitt, A. J., and Li, F.:
Distinguishing nitrous oxide production from nitrification and denitrification on the basis of isotopomer abundances,
Appl. Environ. Microb.,
72, 638–644, https://doi.org/10.1128/aem.72.1.638-644.2006, 2006.
Sutka, R. L., Adams, G. C., Ostrom, N. E., and Ostrom, P. H.:
Isotopologue fractionation during N2O production by fungal denitrification,
Rapid Commun. Mass Sp.,
22, 3989–3996, https://doi.org/10.1002/rcm.3820, 2008.
Tanimoto, T., Hatano, K., Kim, D. H., Uchiyama, H., and Shoun, H.:
Co-denitrification by the denitrifying system of fungus Fusarium oxysporum,
FEMS Microbiol. Lett.,
93, 177–180, 1992.
Toyoda, S. and Yoshida, N.:
Determination of nitrogen isotopomers of nitrous oxide on a modified isotope ratio mass spectrometer,
Anal. Chem.,
71, 4711–4718, https://doi.org/10.1021/ac9904563, 1999.
Toyoda, S., Mutobe, H., Yamagishi, H., Yoshida, N., and Tanji, Y.:
Fractionation of N2O isotopomers during production by denitrifier,
Soil Biol. Biochem.,
37, 1535–1545, https://doi.org/10.1016/j.soilbio.2005.01.009, 2005.
Toyoda, S., Yano, M., Nishimura, S.-i., Akiyama, H., Hayakawa, A., Koba, K., Sudo, S., Yagi, K., Makabe, A., Tobari, Y., Ogawa, N. O., Ohkouchi, N., Yamada, K., and Yoshida, N.:
Characterization and production and consumption processes of N2O emitted from temperate agricultural soils determined via isotopomer ratio analysis,
Global Biogeochem. Cy.,
25, GB2008, https://doi.org/10.1029/2009gb003769, 2011.
USEPA:
Manuel: Nitrogen Control,
Office of Water, Washington, D.C., EPA/625/R-93 /010, 1993.
Well, R. and Flessa, H.:
Isotopologue signatures of N2O produced by denitrification in soils,
J. Geophys. Res.-Biogeo.,
114, G02020, https://doi.org/10.1029/2008jg000804, 2009.
Well, R., Becker, K.-W., Meyer, B., Langel, R., and Reineking, A.:
Continuous flow equilibration for mass spectrometric analysis of dinitrogen emissions,
Soil Sci. Soc. Am. J.,
62, 906–910, https://doi.org/10.2136/sssaj1998.03615995006200040008x, 1998.
Well, R., Kurganova, I., de Gerenyu, V. L., and Flessa, H.:
Isotopomer signatures of soil-emitted N2O under different moisture conditions – A microcosm study with arable loess soil,
Soil Biol. Biochem.,
38, 2923–2933, https://doi.org/10.1016/j.soilbio.2006.05.003, 2006.
Well, R., Burkart, S., Giesemann, A., Grosz, B., Köster, J. R., and Lewicka-Szczebak, D.:
Improvement of the 15N gas flux method for in situ measurement of soil denitrification and its product stoichiometry,
Rapid Commun. Mass Sp.,
33, 437–448, https://doi.org/10.1002/rcm.8363, 2019.
Wrage, N., Velthof, G. L., Laanbroek, H. J., and Oenema, O.:
Nitrous oxide production in grassland soils: assessing the contribution of nitrifier denitrification,
Soil Biol. Biochem.,
36, 229–236, https://doi.org/10.1016/j.soilbio.2003.09.009, 2004a.
Wrage, N., Velthof, G. L., Oenema, O., and Laanbroek, H. J.:
Acetylene and oxygen as inhibitors of nitrous oxide production in Nitrosomonas europaea and Nitrosospira briensis: a cautionary tale,
FEMS Microbiol. Ecol.,
47, 13–18, https://doi.org/10.1016/s0168-6496(03)00220-4, 2004b.
Wrage-Mönnig, N., Horn, M. A., Well, R., Müller, C., Velthof, G., and Oenema, O.:
The role of nitrifier denitrification in the production of nitrous oxide revisited,
Soil Biol. Biochem.,
123, A3–A16, https://doi.org/10.1016/j.soilbio.2018.03.020, 2018.
Wu, D., Well, R., Cárdenas, L. M., Fuß, R., Lewicka-Szczebak, D., Köster, J. R., Brüggemann, N., and Bol, R.:
Quantifying N2O reduction to N2 during denitrification in soils via isotopic mapping approach: Model evaluation and uncertainty analysis,
Environ. Res.,
179, 108806, https://doi.org/10.1016/j.envres.2019.108806, 2019.
Yoshinari, T. and Knowles, R.:
Acetylene inhibition of nitrous-oxide reduction by denitrifying bacteria,
Biochem. Bioph. Res. Co.,
69, 705–710, https://doi.org/10.1016/0006-291x(76)90932-3, 1976.
Yu, L., Harris, E., Lewicka-Szczebak, D., Barthel, M., Blomberg, M. R. A., Harris, S. J., Johnson, M. S., Lehmann, M. F., Liisberg, J., Müller, C., Ostrom, N. E., Six, J., Toyoda, S., Yoshida, N., and Mohn, J.:
What can we learn from N2O isotope data? - Analytics, processes and modelling,
Rapid Commun. Mass Sp.,
e8858, https://doi.org/10.1002/rcm.8858, 2020.
Zou, Y., Hirono, Y., Yanai, Y., Hattori, S., Toyoda, S., and Yoshida, N.:
Isotopomer analysis of nitrous oxide accumulated in soil cultivated with tea (Camellia sinensis) in Shizuoka, central Japan,
Soil Biol. Biochem.,
77, 276–291, https://doi.org/10.1016/j.soilbio.2014.06.016, 2014.
Short summary
This is the first experimental setup combining a complex set of methods (microbial inhibitors and isotopic approaches) to differentiate between N2O produced by fungi or bacteria during denitrification in three soils. Quantifying the fungal fraction with inhibitors was not successful due to large amounts of uninhibited N2O production. All successful methods suggested a small or missing fungal contribution. Artefacts occurring with microbial inhibition to determine N2O fluxes are discussed.
This is the first experimental setup combining a complex set of methods (microbial inhibitors...
Altmetrics
Final-revised paper
Preprint