The Peruvian upwelling system is one of the most productive ecosystems in the ocean. Continuous upwelling of nutrient-rich water generates high primary production, supporting an efficient food web and leading to high fishery yields. An extensive oxygen minimum zone underlying the productive surface waters gives rise to high losses of fixed nitrogen via denitrification and anaerobic ammonium oxidation. This results in nutrient ratios of upwelled waters deviating strongly from Redfield stoichiometry, with a high N deficit and excess P. How this unusual nutrient stoichiometry affects plankton community production and succession and how this feeds back on biogeochemical cycling are still not well understood. To address these questions, an in situ mesocosm experiment was conducted in the coastal upwelling system off Peru in the framework of the Collaborative Research Center 754 "Climate-Biogeochemistry Interactions in the Tropical Ocean". The field experiment took place from January to April 2017 during a coastal El Niño, a rare event of El Niño conditions confined to Peruvian coastal waters. Key objectives of this mesocosm study, which involved 57 researchers from 10 nations, were to unravel the interplay of pelagic ecology and biogeochemistry under the unique environmental conditions of the Peruvian upwelling system. This special issue in Biogeosciences is intended as a collective outlet for publications resulting from this study, covering the broad range of research topics from seawater chemistry to plankton ecology and pelagic biogeochemistry.