Articles | Volume 13, issue 14
Biogeosciences, 13, 4065–4080, 2016
Biogeosciences, 13, 4065–4080, 2016

Research article 18 Jul 2016

Research article | 18 Jul 2016

Nitrogen fixation in sediments along a depth transect through the Peruvian oxygen minimum zone

Jessica Gier1, Stefan Sommer1, Carolin R. Löscher1,a, Andrew W. Dale1, Ruth A. Schmitz2, and Tina Treude1,b Jessica Gier et al.
  • 1GEOMAR Helmholtz Centre for Ocean Research Kiel, Germany
  • 2Institute for Microbiology, Christian-Albrechts-University Kiel, Germany
  • apresent address: Nordic Center for Earth Evolution, University of Southern Denmark, 5230 Odense M, Denmark
  • bpresent address: University of California, Los Angeles, Department of Earth, Planetary & Space Sciences and Department of Atmospheric & Oceanic Sciences, CA, USA

Abstract. The potential coupling of nitrogen (N2) fixation and sulfate reduction (SR) was explored in sediments of the Peruvian oxygen minimum zone (OMZ). Sediment samples were retrieved by a multiple corer at six stations along a depth transect (70–1025 m water depth) at 12° S, covering anoxic and hypoxic bottom water conditions. Benthic N2 fixation, determined by the acetylene reduction assay, was detected at all sites, with highest rates between 70 and 253 m and lower rates at greater depth. SR rates decreased with increasing water depth. N2 fixation and SR overlapped in sediments, suggesting a potential coupling of both processes. However, a weak positive correlation of their activity distribution was detected by principle component analysis. A potential link between N2 fixation and sulfate-reducing bacteria was indicated by the molecular analysis of nifH genes. Detected nifH sequences clustered with the sulfate-reducing bacteria Desulfonema limicola at the 253 m station. However, nifH sequences of other stations clustered with uncultured organisms, Gammaproteobacteria, and Firmicutes (Clostridia) rather than with known sulfate reducers. The principle component analysis revealed that benthic N2 fixation in the Peruvian OMZ is controlled by organic matter (positive) and free sulfide (negative). No correlation was found between N2 fixation and ammonium concentrations (even at levels > 2022 µM). N2 fixation rates in the Peruvian OMZ sediments were in the same range as those measured in other organic-rich sediments.

Short summary
Benthic nitrogen fixation and sulfate reduction were investigated in the Peruvian oxygen minimum zone. The data suggest a coupling of both activities to a large extent, but that also sulfide and organic matter availability are controlling the benthic diazotrophy in this area. The molecular analysis confirms the presence of heterotrophic diazotrophs. This work improves our understanding of N cycling in OMZ sediments and the understanding of N sources in the marine environment.
Final-revised paper