Articles | Volume 15, issue 5
Research article
15 Mar 2018
Research article |  | 15 Mar 2018

Distribution and drivers of symbiotic and free-living diazotrophic cyanobacteria in the western tropical South Pacific

Marcus Stenegren, Andrea Caputo, Carlo Berg, Sophie Bonnet, and Rachel A. Foster


Interactive discussion

Status: closed
Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
Printer-friendly Version - Printer-friendly version Supplement - Supplement

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision
ED: Reconsider after major revisions (14 Dec 2017) by Gerhard Herndl
AR by Marcus Stenegren on behalf of the Authors (14 Dec 2017)  Author's response    Manuscript
ED: Publish subject to technical corrections (09 Feb 2018) by Gerhard Herndl
Short summary
We successfully performed quantitative PCR at sea. The qPCR data were procured within 3 h and used in decisions on further sampling on site. We designed and applied a new primer and probe set for quantifying the UCYN-A1 host and observed discrepancies between host and symbiont, which contradict previous studies. Lastly, we observed a clear vertical separation between a subsurface group (UCYN-A with hosts) and a surface group (remaining diazotrophs), mainly separated by temperature.
Final-revised paper