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Articles | Volume 15, issue 5
Articles | Volume 15, issue 5
https://doi.org/10.5194/bg-15-1559-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 3.0 License.
Special issue:
https://doi.org/10.5194/bg-15-1559-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 3.0 License.
Articles | Volume 15, issue 5
Research article
 | 
15 Mar 2018
Research article |  | 15 Mar 2018

Distribution and drivers of symbiotic and free-living diazotrophic cyanobacteria in the western tropical South Pacific

Marcus Stenegren, Andrea Caputo, Carlo Berg, Sophie Bonnet, and Rachel A. Foster

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Short summary
We successfully performed quantitative PCR at sea. The qPCR data were procured within 3 h and used in decisions on further sampling on site. We designed and applied a new primer and probe set for quantifying the UCYN-A1 host and observed discrepancies between host and symbiont, which contradict previous studies. Lastly, we observed a clear vertical separation between a subsurface group (UCYN-A with hosts) and a surface group (remaining diazotrophs), mainly separated by temperature.
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