Articles | Volume 15, issue 5
Research article
15 Mar 2018
Research article |  | 15 Mar 2018

Distribution and drivers of symbiotic and free-living diazotrophic cyanobacteria in the western tropical South Pacific

Marcus Stenegren, Andrea Caputo, Carlo Berg, Sophie Bonnet, and Rachel A. Foster

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Cited articles

Aminot, A. and Kérouel, R.: Dosage automatique des nutriments dans les eaux marines: méthodes en flux continu, in: Méthodes d'analyse en milieu marin, Ifremer, p. 188, available at: (last access: 1 December 2016), 2007. 
Benavides, M., Bonnet, S., Hernández, N., Martínez-Pérez, A. M., Nieto-Cid, M., Álvarez-Salgado, X. A., Baños, I., Montero, M. F., Mazuecos, I. P., Gasol, J. M., Osterholz, H., Dittmar, T., Berman-Frank, I., and Arístegui, J.: Basin-wide N2 fixation in the deep waters of the Mediterranean Sea, Global Biogeochem. Cy., 30, 952–961,, 2016. 
Bench, S. R., Heller, P., Frank, I., Arciniega, M., Shilova, I. N., and Zehr, J. P.: Whole genome comparison of six Crocosphaera watsonii strains with differing phenotypes, J. Phycol., 49, 786–801,, 2013. 
Berman-Frank, I., Lundgren, P., Chen, Y.-B., Küpper, H., Kolber, Z., Bergman, B., and Falkowski, P.: Segregation of Nitrogen Fixation and Oxygenic Photosynthesis in the Marine Cyanobacterium Trichodesmium, Science, 294, 1534–1537,, 2001. 
Berthelot, H., Moutin, T., L'Helguen, S., Leblanc, K., Hélias, S., Grosso, O., Leblond, N., Charrière, B., and Bonnet, S.: Dinitrogen fixation and dissolved organic nitrogen fueled primary production and particulate export during the VAHINE mesocosm experiment (New Caledonia lagoon), Biogeosciences, 12, 4099–4112,, 2015. 
Short summary
We successfully performed quantitative PCR at sea. The qPCR data were procured within 3 h and used in decisions on further sampling on site. We designed and applied a new primer and probe set for quantifying the UCYN-A1 host and observed discrepancies between host and symbiont, which contradict previous studies. Lastly, we observed a clear vertical separation between a subsurface group (UCYN-A with hosts) and a surface group (remaining diazotrophs), mainly separated by temperature.
Final-revised paper