Articles | Volume 21, issue 2
Research article
26 Jan 2024
Research article |  | 26 Jan 2024

Continental scientific drilling and microbiology: (extremely) low biomass in bedrock of central Sweden

George Westmeijer, Cristina Escudero, Claudia Bergin, Stephanie Turner, Magnus Ståhle, Maliheh Mehrshad, Prune Leroy, Moritz Buck, Pilar López-Hernández, Jens Kallmeyer, Ricardo Amils, Stefan Bertilsson, and Mark Dopson

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Cited articles

Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R., and Stahl, D. A.: Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations, Appl. Environ. Microbiol., 56, 1919–1925,, 1990. 
Bell, E., Lamminmäki, T., Alneberg, J., Andersson, A. F., Qian, C., Xiong, W., Hettich, R. L., Frutschi, M., and Bernier-Latmani, R.: Active sulfur cycling in the terrestrial deep subsurface, ISME J., 14, 1260–1272,, 2020. 
Bell, E., Lamminmäki, T., Alneberg, J., Qian, C., Xiong, W., Hettich, R. L., Frutschi, M., and Bernier-Latmani, R.: Active anaerobic methane oxidation and sulfur disproportionation in the deep terrestrial subsurface, ISME J., 16, 1583–1593,, 2022. 
Short summary
Rock cores down to 2250 m depth, groundwater-bearing fractures, and drilling fluid were sampled for DNA to characterize the subsurface microbial community. In general, microbial biomass was extremely low despite the employment of detection methods widespread in low-biomass environments. The described contamination control measures could support future sampling efforts, and our findings emphasize the use of sequencing extraction controls.
Final-revised paper