Articles | Volume 21, issue 2
https://doi.org/10.5194/bg-21-591-2024
https://doi.org/10.5194/bg-21-591-2024
Research article
 | 
26 Jan 2024
Research article |  | 26 Jan 2024

Continental scientific drilling and microbiology: (extremely) low biomass in bedrock of central Sweden

George Westmeijer, Cristina Escudero, Claudia Bergin, Stephanie Turner, Magnus Ståhle, Maliheh Mehrshad, Prune Leroy, Moritz Buck, Pilar López-Hernández, Jens Kallmeyer, Ricardo Amils, Stefan Bertilsson, and Mark Dopson

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Short summary
Rock cores down to 2250 m depth, groundwater-bearing fractures, and drilling fluid were sampled for DNA to characterize the subsurface microbial community. In general, microbial biomass was extremely low despite the employment of detection methods widespread in low-biomass environments. The described contamination control measures could support future sampling efforts, and our findings emphasize the use of sequencing extraction controls.
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