Articles | Volume 13, issue 16
Biogeosciences, 13, 4697–4705, 2016
Biogeosciences, 13, 4697–4705, 2016

Research article 22 Aug 2016

Research article | 22 Aug 2016

Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition

Lisa Thieme1,2, Daniel Graeber3, Martin Kaupenjohann1, and Jan Siemens2 Lisa Thieme et al.
  • 1Chair of Soil Science, Department of Ecology, Technical University of Berlin, Berlin, Germany
  • 2Chair of Soil Resources, Institute of Soil Science and Soil Conservation, iFZ Research Centre for Biosystems, Land Use and Nutrition, Justus-Liebig University Giessen, Giessen, Germany
  • 3Department of Bioscience, Catchment Science and Environmental Management, Aarhus University, Silkeborg, Denmark

Abstract. Freezing can affect concentrations and spectroscopic properties of dissolved organic matter (DOM) in water samples. Nevertheless, water samples are regularly frozen for sample preservation. In this study we tested the effect of different freezing methods (standard freezing at −18 °C and fast-freezing with liquid nitrogen) on DOM concentrations measured as organic carbon (DOC) concentrations and on spectroscopic properties of DOM from different terrestrial ecosystems (forest and grassland). Fresh and differently frozen throughfall, stemflow, litter leachate and soil solution samples were analyzed for DOC concentrations, UV-vis absorption and fluorescence excitation–emission matrices combined with parallel factor analysis (PARAFAC). Fast-freezing with liquid nitrogen prevented a significant decrease of DOC concentrations observed after freezing at −18 °C. Nonetheless, the share of PARAFAC components 1 (EXmax < 250 nm (340 nm), EXmax: 480 nm) and 2 (EXmax: 335 nm, EXmax: 408 nm) to total fluorescence and the humification index (HIX) decreased after both freezing treatments, while the shares of component 3 (EXmax: < 250 nm (305 nm), EXmax: 438 nm) as well as SUVA254 increased. The contribution of PARAFAC component 4 (EXmax: 280 nm, EXmax: 328 nm) to total fluorescence was not affected by freezing. We recommend fast-freezing with liquid nitrogen for preservation of bulk DOC concentrations of samples from terrestrial sources, whereas immediate measuring is preferable to preserve spectroscopic properties of DOM.

Short summary
Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water samples are regularly frozen for sample preservation. To test, if fast-freezing with liquid nitrogen instead of normal freezing at −18 °C can prevent changes in DOM characteristics, we compared fresh and differently frozen terrestrial water samples. We found that fast-freezing with liquid nitrogen can prevent bulk organic matter concentrations but not its spectroscopic properties.
Final-revised paper