Articles | Volume 13, issue 16
https://doi.org/10.5194/bg-13-4697-2016
https://doi.org/10.5194/bg-13-4697-2016
Research article
 | 
22 Aug 2016
Research article |  | 22 Aug 2016

Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition

Lisa Thieme, Daniel Graeber, Martin Kaupenjohann, and Jan Siemens

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AR: Author's response | RR: Referee report | ED: Editor decision
ED: Publish subject to minor revisions (Editor review) (06 Jul 2016) by Tina Treude
AR by L. Thieme on behalf of the Authors (19 Jul 2016)  Author's response    Manuscript
ED: Publish as is (20 Jul 2016) by Tina Treude
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Short summary
Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water samples are regularly frozen for sample preservation. To test, if fast-freezing with liquid nitrogen instead of normal freezing at −18 °C can prevent changes in DOM characteristics, we compared fresh and differently frozen terrestrial water samples. We found that fast-freezing with liquid nitrogen can prevent bulk organic matter concentrations but not its spectroscopic properties.
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