Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter
concentrations, but not its composition

Thieme, Lisa; Graeber, Daniel; Kaupenjohann, Martin; Siemens, Jan

doi:https://doi.org/10.5194/bg-13-4697-2016

Articles | Volume 13, issue 16

https://doi.org/10.5194/bg-13-4697-2016

© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

https://doi.org/10.5194/bg-13-4697-2016

© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

Articles | Volume 13, issue 16

Research article

|

22 Aug 2016

Research article |

| 22 Aug 2016

Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition

Lisa Thieme, Daniel Graeber, Martin Kaupenjohann, and Jan Siemens

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Final revised paper (published on 22 Aug 2016)
Supplement to the final revised paper
Preprint (discussion started on 22 Mar 2016)
Supplement to the preprint

Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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RC1: 'Comment to Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition', Anonymous Referee #1, 30 Mar 2016
- AC1: 'reply to reviewer #1', L. Thieme, 15 Jun 2016
RC2: 'Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition', Anonymous Referee #2, 14 Apr 2016
- AC2: 'reply to referee 2', L. Thieme, 15 Jun 2016
RC3: 'Thieme freezing DOM review', Anonymous Referee #3, 17 May 2016
- AC3: 'reply to referee 3', L. Thieme, 15 Jun 2016

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

ED: Publish subject to minor revisions (Editor review) (06 Jul 2016) by Tina Treude

AR by L. Thieme on behalf of the Authors (19 Jul 2016) Author's response Manuscript

ED: Publish as is (20 Jul 2016) by Tina Treude

AR by L. Thieme on behalf of the Authors (29 Jul 2016)

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Short summary

Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water samples are regularly frozen for sample preservation. To test, if fast-freezing with liquid nitrogen instead of normal freezing at −18 °C can prevent changes in DOM characteristics, we compared fresh and differently frozen terrestrial water samples. We found that fast-freezing with liquid nitrogen can prevent bulk organic matter concentrations but not its spectroscopic properties.