Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter
concentrations, but not its composition

Thieme, Lisa; Graeber, Daniel; Kaupenjohann, Martin; Siemens, Jan

doi:10.5194/bg-13-4697-2016

Articles | Volume 13, issue 16

https://doi.org/10.5194/bg-13-4697-2016

© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

https://doi.org/10.5194/bg-13-4697-2016

© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

Articles | Volume 13, issue 16

Research article

|

22 Aug 2016

Research article |

| 22 Aug 2016

Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition

Lisa Thieme, Daniel Graeber, Martin Kaupenjohann, and Jan Siemens

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Mar 2020	6	20	11	37
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Cumulative views and downloads (calculated since 22 Mar 2016)

Month	HTML	PDF	XML	Total
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May 2016	12	10	0	22
Jun 2016	5	2	0	7
Jul 2016	5	9	0	14
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Nov 2016	7	6	1	14
Dec 2016	6	2	0	8
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Feb 2017	14	12	0	26
Mar 2017	20	26	2	48
Apr 2017	27	30	0	57
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Jul 2017	13	31	1	45
Aug 2017	6	29	2	37
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Jun 2018	23	35	4	62
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Apr 2019	15	40	23	78
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Mar 2025	11	9	0	20
Apr 2025	17	16	0	33
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Jun 2025	41	22	1	64
Jul 2025	15	14	4	33
Aug 2025	38	14	1	53
Sep 2025	32	16	2	50
Oct 2025	31	31	3	65
Nov 2025	33	11	0	44

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Short summary

Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water samples are regularly frozen for sample preservation. To test, if fast-freezing with liquid nitrogen instead of normal freezing at −18 °C can prevent changes in DOM characteristics, we compared fresh and differently frozen terrestrial water samples. We found that fast-freezing with liquid nitrogen can prevent bulk organic matter concentrations but not its spectroscopic properties.