Articles | Volume 13, issue 16
https://doi.org/10.5194/bg-13-4697-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/bg-13-4697-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
Research article
| 
22 Aug 2016
Research article |
| 22 Aug 2016
Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition
Lisa Thieme
CORRESPONDING AUTHOR
Chair of Soil Science, Department of Ecology, Technical University
of Berlin, Berlin, Germany
Chair of Soil Resources, Institute of Soil Science and Soil
Conservation, iFZ Research Centre for Biosystems, Land Use and Nutrition,
Justus-Liebig University Giessen, Giessen, Germany
Daniel Graeber
Department of Bioscience, Catchment Science and Environmental
Management, Aarhus University, Silkeborg, Denmark
Martin Kaupenjohann
Chair of Soil Science, Department of Ecology, Technical University
of Berlin, Berlin, Germany
Jan Siemens
Chair of Soil Resources, Institute of Soil Science and Soil
Conservation, iFZ Research Centre for Biosystems, Land Use and Nutrition,
Justus-Liebig University Giessen, Giessen, Germany
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Cited
18 citations as recorded by crossref.
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- Assessing inputs of aquaculture-derived nutrients to streams using dissolved organic matter fluorescence K. Ryan et al. 10.1016/j.scitotenv.2021.150785
- The optical characteristics and sources of chromophoric dissolved organic matter (CDOM) in seasonal snow of northwestern China Y. Zhou et al. 10.5194/tc-13-157-2019
- La pandemia de COVID-19 como oportunidad para poner de relieve la migración de los trabajadores agrícolas a través de la cobertura mediática M. Șerban & A. Croitoru 10.3989/estgeogr.2022117.117
- Assessment of sample freezing as a preservation technique for analysing the molecular composition of dissolved organic matter in aquatic systems J. Fonvielle et al. 10.1039/D3RA01349A
- High abundance of protein-like fluorescence in the Amerasian Basin of Arctic Ocean: Potential implication of a fall phytoplankton bloom M. Chen et al. 10.1016/j.scitotenv.2017.04.233
- Vacuum freeze-drying of sediment cores: an optimised method for preserving archaeostratigraphic archives R. Enevold et al. 10.15184/aqy.2019.98
- Establishing a terrestrial proxy based on fluorescent dissolved organic matter from sediment pore waters in the East China Sea M. Li et al. 10.1016/j.watres.2020.116005
- Factors controlling the geographical distribution of fluorescent dissolved organic matter in the surface waters of the Pacific Ocean Y. Yamashita et al. 10.1002/lno.10570
- Characterization and bioavailability of rainwater dissolved organic matter at the southeast coast of China using absorption spectroscopy and fluorescence EEM-PARAFAC L. Yang et al. 10.1016/j.ecss.2018.11.002
- Numerical Analysis of a Liquid Nitrogen (LN2) Engine for Efficient Energy Conversion S. Rizvi et al. 10.1021/acsomega.1c00582
- Chromophoric and fluorescent dissolved organic matter in and above the oxygen minimum zone off Peru A. Loginova et al. 10.1002/2016JC011906
- Dissolved organic matter characteristics of deciduous and coniferous forests with variable management: different at the source, aligned in the soil L. Thieme et al. 10.5194/bg-16-1411-2019
- Soil water solutes reduce the critical micelle concentration of quaternary ammonium compounds I. Mulder et al. 10.1007/s11356-020-10188-2
- Development of a universal microinfiltrometer to estimate extent and persistence of soil water repellency as a function of capillary pressure and interface chemical composition N. Sepehrnia et al. 10.2478/johh-2020-0035
- Unequal impacts of unidirectional freeze-thaw on different soil layers and aggregate sizes: Evidenced by microbial communities and CO2 emissions Y. Hu et al. 10.1016/j.catena.2024.108057
- Land-based dissolved organic nitrogen dynamics and bioavailability in Jiaozhou Bay, China K. Li et al. 10.1016/j.ecss.2019.02.045
17 citations as recorded by crossref.
- Spectral Characterization of Dissolved Organic Matter in Seawater and Sediment Pore Water from the Arctic Fjords (West Svalbard) in Summer M. Chen et al. 10.3390/w13020202
- Rapid reduction of Cr(VI) with plant leaves: Implications for ex-situ phytoremediation of chromium-polluted waters in cold region Y. Fu et al. 10.1016/j.jclepro.2023.136086
- Assessing inputs of aquaculture-derived nutrients to streams using dissolved organic matter fluorescence K. Ryan et al. 10.1016/j.scitotenv.2021.150785
- The optical characteristics and sources of chromophoric dissolved organic matter (CDOM) in seasonal snow of northwestern China Y. Zhou et al. 10.5194/tc-13-157-2019
- La pandemia de COVID-19 como oportunidad para poner de relieve la migración de los trabajadores agrícolas a través de la cobertura mediática M. Șerban & A. Croitoru 10.3989/estgeogr.2022117.117
- Assessment of sample freezing as a preservation technique for analysing the molecular composition of dissolved organic matter in aquatic systems J. Fonvielle et al. 10.1039/D3RA01349A
- High abundance of protein-like fluorescence in the Amerasian Basin of Arctic Ocean: Potential implication of a fall phytoplankton bloom M. Chen et al. 10.1016/j.scitotenv.2017.04.233
- Vacuum freeze-drying of sediment cores: an optimised method for preserving archaeostratigraphic archives R. Enevold et al. 10.15184/aqy.2019.98
- Establishing a terrestrial proxy based on fluorescent dissolved organic matter from sediment pore waters in the East China Sea M. Li et al. 10.1016/j.watres.2020.116005
- Factors controlling the geographical distribution of fluorescent dissolved organic matter in the surface waters of the Pacific Ocean Y. Yamashita et al. 10.1002/lno.10570
- Characterization and bioavailability of rainwater dissolved organic matter at the southeast coast of China using absorption spectroscopy and fluorescence EEM-PARAFAC L. Yang et al. 10.1016/j.ecss.2018.11.002
- Numerical Analysis of a Liquid Nitrogen (LN2) Engine for Efficient Energy Conversion S. Rizvi et al. 10.1021/acsomega.1c00582
- Chromophoric and fluorescent dissolved organic matter in and above the oxygen minimum zone off Peru A. Loginova et al. 10.1002/2016JC011906
- Dissolved organic matter characteristics of deciduous and coniferous forests with variable management: different at the source, aligned in the soil L. Thieme et al. 10.5194/bg-16-1411-2019
- Soil water solutes reduce the critical micelle concentration of quaternary ammonium compounds I. Mulder et al. 10.1007/s11356-020-10188-2
- Development of a universal microinfiltrometer to estimate extent and persistence of soil water repellency as a function of capillary pressure and interface chemical composition N. Sepehrnia et al. 10.2478/johh-2020-0035
- Unequal impacts of unidirectional freeze-thaw on different soil layers and aggregate sizes: Evidenced by microbial communities and CO2 emissions Y. Hu et al. 10.1016/j.catena.2024.108057
1 citations as recorded by crossref.
Saved (preprint)
Latest update: 09 Oct 2025
Short summary
Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water samples are regularly frozen for sample preservation. To test, if fast-freezing with liquid nitrogen instead of normal freezing at −18 °C can prevent changes in DOM characteristics, we compared fresh and differently frozen terrestrial water samples. We found that fast-freezing with liquid nitrogen can prevent bulk organic matter concentrations but not its spectroscopic properties.
Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water...
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