Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter
concentrations, but not its composition

Thieme, Lisa; Graeber, Daniel; Kaupenjohann, Martin; Siemens, Jan

doi:10.5194/bg-13-4697-2016

Articles | Volume 13, issue 16

https://doi.org/10.5194/bg-13-4697-2016

© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

https://doi.org/10.5194/bg-13-4697-2016

© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

Articles | Volume 13, issue 16

Research article

|

22 Aug 2016

Research article |

| 22 Aug 2016

Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition

Lisa Thieme, Daniel Graeber, Martin Kaupenjohann, and Jan Siemens

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Mar 2026	27	13	3	43

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Short summary

Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water samples are regularly frozen for sample preservation. To test, if fast-freezing with liquid nitrogen instead of normal freezing at −18 °C can prevent changes in DOM characteristics, we compared fresh and differently frozen terrestrial water samples. We found that fast-freezing with liquid nitrogen can prevent bulk organic matter concentrations but not its spectroscopic properties.