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Volume 13, issue 16
Biogeosciences, 13, 4697–4705, 2016
https://doi.org/10.5194/bg-13-4697-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
Biogeosciences, 13, 4697–4705, 2016
https://doi.org/10.5194/bg-13-4697-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

Research article 22 Aug 2016

Research article | 22 Aug 2016

Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition

Lisa Thieme et al.

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Cited articles

Baker, A., Elliott, S., and Lead, J. R.: Effects of filtration and pH perturbation on freshwater organic matter fluorescence, Chemosphere, 67, 2035–2043, 2007.
Belzile, C., Gibson, J. A. E., and Vincent, W. F.: Colored dissolved organic matter and dissolved organic carbon exclusion from lake ice: Implications for irradiance transmission and carbon cycling, Limnol. Oceanogr., 47, 1283–1293, 2002.
Bolan, N. S., Adriano, D. C., Kunhikrishnan, A., James, T., McDowell, R., and Senesi, N.: Dissolved Organic Matter: Biogeochemistry, Dynamics, and Environmental Significance in Soils, Adv. Agron., 110, 1–75, 2011.
Christ, M. and David, M. B.: Fractionation of dissolved organic carbon in soil water: Effects of extraction and storage methods, Commun. Soil Sci. Plant, 25, 3305–3319, 1994.
Conmy, R. N., Coble, P. G., Cannizzaro, J. P., and Heil, C. A.: Influence of extreme storm events on West Florida Shelf CDOM distributions, J. Geophys. Res.-Biogeo., 114, 1–17, https://doi.org/10.1029/2009JG000981, 2009.
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Short summary
Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water samples are regularly frozen for sample preservation. To test, if fast-freezing with liquid nitrogen instead of normal freezing at −18 °C can prevent changes in DOM characteristics, we compared fresh and differently frozen terrestrial water samples. We found that fast-freezing with liquid nitrogen can prevent bulk organic matter concentrations but not its spectroscopic properties.
Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water...
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