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Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water samples are regularly frozen for sample preservation. To test, if fast-freezing with liquid nitrogen instead of normal freezing at −18 °C can prevent changes in DOM characteristics, we compared fresh and differently frozen terrestrial water samples. We found that fast-freezing with liquid nitrogen can prevent bulk organic matter concentrations but not its spectroscopic properties.
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Articles | Volume 13, issue 16
Biogeosciences, 13, 4697–4705, 2016
https://doi.org/10.5194/bg-13-4697-2016
Biogeosciences, 13, 4697–4705, 2016
https://doi.org/10.5194/bg-13-4697-2016

Research article 22 Aug 2016

Research article | 22 Aug 2016

Fast-freezing with liquid nitrogen preserves bulk dissolved organic matter concentrations, but not its composition

Lisa Thieme et al.

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Cited articles

Baker, A., Elliott, S., and Lead, J. R.: Effects of filtration and pH perturbation on freshwater organic matter fluorescence, Chemosphere, 67, 2035–2043, 2007.
Belzile, C., Gibson, J. A. E., and Vincent, W. F.: Colored dissolved organic matter and dissolved organic carbon exclusion from lake ice: Implications for irradiance transmission and carbon cycling, Limnol. Oceanogr., 47, 1283–1293, 2002.
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Short summary
Freezing can affect dissolved organic matter properties and concentrations. Nevertheless, water samples are regularly frozen for sample preservation. To test, if fast-freezing with liquid nitrogen instead of normal freezing at −18 °C can prevent changes in DOM characteristics, we compared fresh and differently frozen terrestrial water samples. We found that fast-freezing with liquid nitrogen can prevent bulk organic matter concentrations but not its spectroscopic properties.
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