the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
A Bayesian approach to evaluation of soil biogeochemical models
Hua W. Xie
Adriana L. RomeroOlivares
Michele Guindani
Steven D. Allison
To make predictions about the carbon cycling consequences of rising global surface temperatures, Earth system scientists rely on mathematical soil biogeochemical models (SBMs). However, it is not clear which models have better predictive accuracy, and a rigorous quantitative approach for comparing and validating the predictions has yet to be established. In this study, we present a Bayesian approach to SBM comparison that can be incorporated into a statistical model selection framework. We compared the fits of linear and nonlinear SBMs to soil respiration data compiled in a recent metaanalysis of soil warming field experiments. Fit quality was quantified using Bayesian goodnessoffit metrics, including the widely applicable information criterion (WAIC) and leaveoneout cross validation (LOO). We found that the linear model generally outperformed the nonlinear model at fitting the metaanalysis data set. Both WAIC and LOO computed higher overfitting risk and effective numbers of parameters for the nonlinear model compared to the linear model, conditional on the data set. Goodness of fit for both models generally improved when they were initialized with lower and more realistic steadystate soil organic carbon densities. Still, testing whether linear models offer definitively superior predictive performance over nonlinear models on a global scale will require comparisons with additional sitespecific data sets of suitable size and dimensionality. Such comparisons can build upon the approach defined in this study to make more rigorous statistical determinations about model accuracy while leveraging emerging data sets, such as those from longterm ecological research experiments.
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Coupled Earth system models (ESMs) and constituent soil biogeochemical models (SBMs) are used to simulate global soil organic carbon (SOC) dynamics and storage. As global climate changes, some ESM and SBM simulations suggest that substantial SOC losses could occur, resulting in greater soil CO_{2} emissions (Crowther et al., 2016). However, there is vast divergence between model predictions. For instance, one ESM predicts a global SOC loss of 72 Pg C over the 21st century, while another predicts a gain of 253 Pg C (ToddBrown et al., 2014).
Soil biogeochemical models vary greatly in structure (Manzoni and Porporato, 2009) but can be broadly partitioned into two categories: those that implicitly represent soil C dynamics as firstorder linear decay processes and those that explicitly represent microbial control over C dynamics with nonlinear Michaelis–Menten functions (Wieder et al., 2015a). Explicit models typically include more parameters than linear models because multiple microbial parameters are needed for each decay process as opposed to a single rate parameter. The additional parameters allow explicit models to represent microbial mechanisms, but at the expense of greater model complexity.
Rigorous statistical approaches should be applied to investigate how explicit representation of microbial processes affects predictive model performance. ESM and SBM comparisons involving empirical soil C data assimilations have been conducted previously (Allison et al., 2010; Li et al., 2014) but few standardized statistical methods for ESM and SBM benchmarking and comparison have been developed that would allow for rigorous model selection. Prior model comparisons have involved graphical qualitative comparisons or use of basic fit metrics such as the coefficient of determination, R^{2}, to judge fit quality. However, these simple approaches are insufficient for comparing an increasing number of complex models (Jiang et al., 2015; Luo et al., 2016; Wieder et al., 2015a).
R^{2} on its own provides limited information about goodness of fit. In unmodified form, it quantifies the extent to which the variation of just one chosen model outcome – for instance the mean outcome for a range of parameter values – corresponds to the variation in the data set (Gelman et al., 2019). R^{2} does not capture model complexity, overfitting, or parameter uncertainty, which is a reason why R^{2} by itself is not sufficient for model evaluation (Kvålseth, 1985). Without accounting for model complexity and parameter count, focusing on optimizing fit by R^{2} values alone can easily lead to overfitting (Spiess and Neumeyer, 2010).
Encouragingly, a rich toolset to further inform quantitative model evaluation and comparison can be drawn from Bayesian statistics (Hararuk et al., 2014, 2018; Hararuk and Luo, 2014). These tools include information criteria and approximate crossvalidation, goodnessoffit metrics designed for the simultaneous comparison of multiple structurally diverse models. Like R^{2}, information criteria and cross validation are quantitative measures that estimate the fit quality of a model to a given data set. Differing from R^{2}, information criteria and cross validation are relative rather than absolute measures. These metrics evaluate the extent to which the data set supports particular distributions of parameter values and, in turn, the uncertainty of parameter estimates. Consequently, if the distribution of model A outcomes aligns more closely to the data set than the distribution of model B outcomes, we regard model A as being more likely to explain the data compared to model B. Information criteria and crossvalidation metrics also typically include terms penalizing for model complexity and overfitting as part of their computation (Gelman et al., 2014). Hence, information criteria and approximate cross validation are useful tools for model evaluation because they present a comprehensive summary of model fit to time series data and can estimate model predictive accuracy for unmeasured and outofsample data points.
Examples of information criteria popularized by widely used R packages such as lme4 and rjags include the Akaike information criterion (AIC), Bayesian information criterion (BIC), and deviance information criterion (DIC) (Vehtari and Ojanen, 2012). However, these metrics have some limitations. AIC, BIC, and DIC do not use full sampled posterior distributions in their computational processes. AIC and BIC both rely on a pointwise maximum likelihood estimate that cannot be derived from nonuniform Bayesian prior distributions, including normal distributions. AIC and BIC (despite BIC's name) thereby have limited use in Bayesian statistics settings. DIC can accommodate nonuniform priors but is calculated from pointwise simplified posterior means. The compression of full posteriors into pointwise means can prompt DIC to compute an impossible negative effective model parameter count in select situations (Gelman et al., 2014). Consequently, the original forms of AIC, BIC, and DIC are no longer recommended for use in Bayesian model assessment by some statisticians in light of superseding alternatives (Gelman et al., 2014).
Three predictive goodnessoffit metrics address the limitations and stability issues of AIC, BIC, and DIC by incorporating full, nonuniform posterior distributions in their calculations to better account for overfitting and model size (Christensen et al., 2010; Gelman et al., 2014). These metrics include the widely applicable information criterion (WAIC), log pseudomarginal likelihood (LPML), and Paretosmoothed important sampling leaveoneout cross validation (PSISLOO and hereby referred to as LOO). WAIC, LPML, and LOO can estimate the ability of models to fit unobserved measurements outside of the set of measured data samples (Vehtari et al., 2017). Thus, WAIC, LPML, and LOO can be considered as superior barometers for model predictive accuracy compared to AIC, BIC, and DIC.
The overarching goal of this study was to develop a statistically rigorous and mathematically consistent data assimilation framework for SBM comparison that uses predictive Bayesian goodnessoffit metrics. We pursued three specific objectives as part of that goal. First, we compared the behaviors of two different SBMs, a linear microbialimplicit model termed the conventional model (CON) and a nonlinear microbialexplicit model called the Allison–Wallenstein–Bradford model (AWB) (Fig. 1), following data assimilation with soil respiration data sourced from a metaanalysis of soil warming studies (RomeroOlivares et al., 2017). Second, we characterized the parameter spaces of these models using prior probability distributions of parameter values informed by previous studies and expert judgment. Third, we compared specific Bayesian predictive information criteria in WAIC, LPML, and LOO to the coefficient of determination, R^{2}, for quantifying goodness of fit to data. AIC, BIC, and DIC were not analyzed due to their stability limitations, our usage of nonuniform prior distributions, and redundancy with WAIC.
2.1 Model structures
We compared two SBMs, the CON and AWB models (Allison et al., 2010). The models were selected for this study due to their relative equation simplicity, their tractable parameter count, and limited biological data input requirements (Sect. S1 in the Supplement). The CON system models three separate C pools as state variables including SOC, dissolved organic C (DOC), and microbial biomass C (MIC) pools, while AWB includes SOC, DOC, MIC, and extracellular enzyme biomass C (ENZ) pools (Fig. 1). Additionally, these models were chosen because they are Conly models without nitrogen (N) pools. The increased complexity of Naccounting SBMs will require future studies with coupled N data sets (Manzoni and Porporato, 2009).
2.2 Metaanalysis data
The data set for model fitting was compiled from a recent metaanalysis of 27 soil warming studies that measured CO_{2} fluxes (RomeroOlivares et al., 2017). The experiments reported between 1 and 13 years of CO_{2} flux measurements following warming perturbation. The elements of this data set consisted of empirical response ratios calculated by dividing CO_{2} fluxes measured in the warming treatments by timepaired CO_{2} fluxes measured in the control treatments. We calculated an annual mean response ratio for each experiment (if data were available for that year) after warming treatment began. Using these annual means, we calculated one overall mean response ratio for each year along with pooled variances and standard deviations. Pooled data points were assumed to be “collected” at the halfway point of each year. Because the experiments had variable lengths, the sample size for the pooled annual mean declines with increasing time since warming perturbation. The warming perturbation was 3 ^{∘}C on average across all the studies, and this average was used as the magnitude of warming in the model simulations.
Modeloutputted response ratios were calculated by dividing simulated CO_{2} flux following warming perturbation by the CO_{2} flux at prewarming steady state. We fit models to flux response ratios rather than raw flux measurements for several reasons (Wieder et al., 2015b). First, we eliminate the need to convert flux measurements from different experiments into a common unit. Second, response ratios represent a standardized metric for warming response across disparate ecosystem types with varying climate, soil, and vegetation properties. Finally, fitting a mean response ratio overcomes data gaps present in individual experiments.
2.3 Hamiltonian Monte Carlo fitting of differential equation models
CON and AWB ordinary differential equation systems were simulated using the CVODE backward differentiation method (Curtiss and Hirschfelder, 1952) from the SUNDIALS library of equation solvers (Hindmarsh et al., 2005). Differential equation models contain parameters that affect state variables, and model fitting through Markov chain algorithms involves iterating through parameter space one set of parameters at a time. We performed model fitting using a Markov chain algorithm called the Hamiltonian Monte Carlo (HMC), using version 2.18.1 of the RStan interface to the Stan statistical software (Carpenter et al., 2017; Stan Development Team, 2020) and version 3.4.1 of R (R Core Team, 2017). HMC is not a randomwalk algorithm and uses Hamiltonian mechanics to determine exploration steps in parameter space. HMC has been theorized to offer more efficient exploration of highdimensional parameter space than traditional randomwalk Metropolis algorithms (Beskos et al., 2013).
Conditional on the metaanalysis data set, the HMC algorithm computed posterior and posterior predictive distributions, from which Bayesian statistical inferences on likely ranges of parameter values were then made. Posterior distributions are the distributions of more likely model parameter values conditional on the data. Posterior predictive distributions are the distributions of more likely values for unobserved data points from the datagenerating process conditional on the observations. In the case of this study, the experiments constituting the metaanalysis would be the datagenerating process.
For the sake of clarity, it is important to distinguish between the frequentist confidence intervals and Bayesian posterior predictive intervals and distributions we describe in our study. Confidence intervals are calculated from the sample means and standard errors at observed data points and indicate ranges of values that are likely to contain the true data values with repeated sample collections using the same methodology. Posterior predictive intervals and distributions are computed after estimation of the posterior parameter distributions and represent the likely distributions of unobserved data values conditional on observed data values. Bayesian credible intervals, which we will also discuss in this study, are ranges of values that parameters are likely to take with some probability that are conditional on the observed data. Credible areas indicate the probability densities of parameter values across credible intervals.
We ran four chains for 35 000 iterations each for our HMC simulations, with the first 10 000 iterations being discarded as burnin for each chain. Hence, our posterior distributions consisted of 100 000 posterior samples per HMC run. In retrospect, because our credible areas displayed sufficient smoothness (Fig. S2 in the Supplement) and Bayesian diagnostics indicated adequate posterior sampling (Table S5 in the Supplement), we could have reduced simulation time without impairing posterior computation by running shorter chains that consisted of 20 000 to 30 000 iterations. To minimize the presence of divergent energy transitions, which indicate issues with exploring the geometry of the parameter space specified by the prior distributions, we set the adaptation delta to 0.95, the initial step size to 0.1, and maximum tree depth to 12. Those parameters determine how the HMC algorithm proposes new sets of parameters at each step and were set so that the HMC would begin with smaller exploration steps. The algorithm varies the step size from its initial value throughout posterior sampling to maintain a desired acceptance rate; the tuning sensitivity of the step size is governed by the adaptation delta value, with higher values indicating reduced sensitivity.
We further constrained our HMC runs to characterize parameter regimes corresponding to higher biological realism. Normal informative priors were used to initiate the runs, and the prior distribution parameters were chosen based on expert opinion and previous empirical observations (Allison et al., 2010; Li et al., 2014). Prior distributions had noninfinite supports; supports were truncated to prevent the HMC from exploring parameter space that was unrealistic (Table S2).
2.4 Model steadystate initialization
Because we were mainly interested in testing model predictions of soil warming response, the models were initiated at steadystate prior to the introduction of warming perturbation to isolate model warming responses from steadystate attraction. We fixed preperturbation steadystate soil C densities to prevent HMC runs from exploring parameter regimes corresponding to biologically unrealistic C pool densities and mass ratios.
To set prewarming steadystate soil C densities, we first analytically derived steadystate solutions of the ordinary differential equations of the models. Then, with the assistance of Mathematica version 12, we rearranged the equations by moving the steadystate pool sizes to the lefthand side (Sect. S2), such that we could determine the value of parameters dependent on pool sizes while allowing the rest of the parameters to vary for the HMC. Consequently, we could constrain the prewarming pool sizes from reaching unrealistic values in the simulations.
2.5 Sensitivity analysis of C pool ratios
Sensitivity analyses examine how the distributions of model input values influence the distributions of model outputs. In our study, we considered prewarming C pool densities as a model input. We performed a sensitivity analysis to observe how the choice of prewarming C pool densities and C pool ratios would affect the model fits and posterior predictive distribution of C pool ratios.
We compared the model outputs and postwarming response behavior of AWB and CON at equivalent C pool densities and ratios. The ratio of soil microbe biomass C (MIC) density to SOC density has been observed to vary approximately from 0.01 to 0.04 (Anderson and Domsch, 1989; Sparling, 1992), so we used those numbers as guidelines for establishing the ranges of the C pool densities and density ratios explored in our simulations. One portion of the analysis involved running HMC simulations in which we set the prewarming MIC density at 2 mg C g^{−1} soil and then varied the SOC density from 50 to 200 mg C g^{−1} soil in increments of 25, stepping from 0.04 to 0.01 with respect to the MICtoSOC ratio. A second portion of the analysis involved observing the effect of varying prewarming MIC from 1 to 8 mg C g^{−1} soil while holding prewarming SOC at 100 mg C g^{−1} soil.
For some combinations of the prior distributions and prewarming steadystate C pool densities (Table S2), AWB HMC runs wandered into unstable parameter regimes that would prevent the algorithm from reliably running to completion. Consequently, we do not compare simulation results for AWB and CON with prewarming SOC densities below 50 mg C g^{−1} soil. Other combinations of prior distribution and prewarming C pool density choices that were not necessarily biologically realistic allowed stable AWB runs with lower prewarming SOC densities.
2.6 Information criteria and cross validation
In addition to R^{2}, we used the WAIC, LPML, and LOO Bayesian predictive goodnessoffit metrics to evaluate models with the metaanalysis warming response data. LPML is an example of cross validation that is calculated similarly to LOO (Gelfand et al., 1992; Gelfand and Dey, 1994; Ibrahim et al., 2001) but differs from LOO in how the importance ratio sampling portion of its computation is handled. For further explanation regarding importance ratios and their role in evaluating approximate crossvalidation metrics, refer to the description of the LOO algorithm presented in Vehtari and Ojanen (2012). LOO updates LPML by implementing a smoothing process in which the largest importance ratios are fitted with a Pareto distribution and then replaced by expected values from the distribution, which stabilizes the importance ratio sampling.
Algorithmic differences between WAIC and LPML and LOO render them appropriate for different statistical modeling goals and make them complementary metrics. WAIC is suitable for estimating the relative quality of model fits to hypothetical repeated samples collected at existing experimental time points, whereas LOO and LPML are suitable for estimating the quality of fits to hypothetical measurements taken between observed time points (Vehtari et al., 2017).
We used version 2.0.0 of the “loo” package available for R to calculate our WAIC and LOO values (Vehtari et al., 2019). A lower WAIC and LOO and a higher LPML indicate a more likely model for a given data set. LPML can be multiplied by a factor of −2 to occupy a similar scale to LOO.
3.1 Parameter posterior distributions
We obtained distributions of posterior predictive fits to the univariate response ratio data for both AWB and CON across different prewarming MICtoSOC ratios. Posterior samples totaled 100 000 for each simulation. Sampler diagnostics for the HMC runs indicated that the statistical models were valid at all prewarming steadystate values observed (Table S6), that model parameter values converged across the four Markov chains (Fig. S7), and that the posterior parameter space was effectively sampled and explored (Fig. S5) to generate enough independent posterior samples for inference (Fig. S6). The ratios of effective posterior parameter samples to total samples for parameters were generally satisfactory; across observed MICtoSOC ratios, they were all greater than 0.25 and mostly greater than 0.5 (Table S5).
We also tracked divergent transitions, which mark points in chains at which the HMC algorithm was inhibited in its exploration and posterior sampling, potentially due to the parameter space becoming geometrically confined and difficult to navigate. Divergent transitions occurred in the AWB HMC runs (Fig. S9), though the ratios of divergent transitions to sampled iterations were relatively low for all runs. The highest divergent transition ratio observed was 0.0217, corresponding to the simulation initiated with prewarming SOC = 200 mg C g^{−1} soil. There were no divergent transitions in the CON runs.
3.2 Model behaviors
The CON curve monotonically decreases in response ratio over time, whereas the AWB curve displays changes in slope sign (Fig. 2). The difference in curve shape (Fig. 3a, b) is in line with CON's linear status and AWB's nonlinear formulation with more parameters (Allison et al., 2010). By 50 years after warming, mean fit curves for AWB and CON return to 1.0 after their initial increase (Fig. 3c, d), consistent with prior observations and expectations at steady state (van Gestel et al., 2018; RomeroOlivares et al., 2017).
From a cursory visual evaluation, neither of the models clearly outperforms the other across all prewarming steady states. The 95 % confidence interval of the first data point at t=0.5 years does not include the AWB SOC100 posterior predictive mean as it does for the CON SOC100 mean (Fig. 2), which most likely impaired AWB's quantitative goodnessoffit metrics. However, the 95 % response ratio posterior predictive interval suggests that AWB is able to replicate the response ratio increase in the data from 1.5 to 3.5 years following the warming perturbation, which CON does not. The shape of the AWB posterior predictive interval also fits the data points and confidence intervals occurring 8 years or more after the perturbation more closely than that of CON (Fig. 3a, b).
For both AWB and CON, increasing the prewarming SOC to higher densities from SOC = 50 to 200 mg C g^{−1} soil (hereby labeled from SOC50 to SOC200) while holding prewarming MIC at 2 mg C g^{−1} soil, DOC at 0.2 mg C g^{−1} soil, and ENZ at 0.1 mg C g^{−1} soil corresponded to lower initial mean response ratios in the first year at the t=0.5 year time point, which certainly inhibited the quantitative goodness of fit (Fig. 3a, b). For CON, increasing prewarming SOC also reduced the magnitude of the mean fit slope. For AWB, increasing prewarming SOC had no clear effect on the curve slope, but the model needed more time to achieve peak mean response ratio from a lower start, with the peak being reached at t=1.5 years in the SOC50 case and t=3.5 years in the SOC200 case (Fig. 3b). At higher prewarming SOC, CON's reduced slope magnitude and AWB's lagging response ratio peak caused both models to exhibit slower returns to the steadystate response ratio of 1.0 (Fig. 3c, d). On their trajectories back to steady state, the mean SOC200 CON curve substantially overshoots the data means after t=7.5 years (Fig. 3a), whereas the SOC200 AWB curve exceeds the data means at a more moderate extent through the t=8.5, 9.5, 10.5, and 11.5 year time points (Fig. 3b).
Changing the prewarming MICtoSOC steadystate pool size ratio by increasing prewarming MIC from 1 to 8 mg C g^{−1} soil (hereby labeled from MIC1 to MIC8) while holding prewarming SOC at 100 mg C g^{−1} soil had marginal to moderate qualitative effects on the mean response ratio curves for CON and AWB. The CON MIC1 and MIC8 curves are visually indistinguishable (Fig. S1a, b), while the AWB MIC1 and MIC8 curves differ with the MIC8 curve displaying more gradual changes in slope and lower slope magnitudes (Fig. S1c, d).
3.3 Sensitivity analysis of parameter distributions to prewarming C pool densities and density ratios
In addition to response ratio fits, we observed the influence of prewarming MICtoSOC ratios on model SOC stock response ratios in AWB and CON simulations following warming. Similar to the model flux response ratios, SOC response ratios were calculated by dividing evolved postwarming SOC densities by prewarming densities. The SOC response ratios at 12.5 years for CON and AWB increased as prewarming SOC was raised (and, hence, the MICtoSOC ratio decreased) with other prewarming C densities held constant, indicating reduced proportional SOC loss when SOC stocks were initiated at higher prewarming densities (Fig. S3a). For CON, SOC loss decreased from 27.1 % at SOC50 to 9.2 % at SOC200. In a similar trend for AWB, SOC loss decreased from 17.2 % at SOC50 to 8.1 % at SOC200. In contrast, raising prewarming MIC densities (and, hence, increasing the MICtoSOC ratio) with other prewarming C densities held constant did not produce a shared trend for CON and AWB (Fig. S3b). CON SOC loss decreased from 18.8 % at MIC1 to 17.4 % at MIC8, while AWB SOC loss increased from 11.3 % at MIC1 to 16.3 % at MIC8.
Truncation of prior supports, or distribution domains, generally did not prevent posterior densities from retaining normal distribution shapes. Deformation away from Gaussian shapes for the densities of Ea_{S} from CON was observed at SOC50 and SOC75. For AWB, deformation was observed for the densities of Ea_{V}, Ea_{K}, and ${E}_{{\mathrm{C}}_{\mathrm{ref}}}$. All CON and AWB parameter posterior densities were otherwise observed to be Gaussian from SOC100 to SOC200. Example posterior densities and means for select model parameters at prewarming SOC100 are presented in Figs. 4 and S2. Parameter posterior means corresponding to other prewarming C pool densities and ratios are presented in Table S3.
3.4 Sensitivity analysis of quantitative fit metrics to prewarming C pool densities and density ratios
For both CON and AWB, LOO, WAIC, LPML, and R^{2} all worsened as prewarming steadystate SOC density was increased from SOC50 to the less biologically realistic SOC200 (Fig. 5). CON's LOO and WAIC values increased respectively from −15.704 and −15.818 at SOC50 to −6.891 and −6.966 at SOC200, while AWB's LOO and WAIC values increased respectively from −11.028 and −11.379 at SOC50 to −5.97 and −6.579 at SOC200 (Table S4a, b). Compared to AWB's metrics, CON's goodnessoffit metrics deteriorated at a faster rate with the increase in prewarming SOC. Nonetheless, CON outperformed AWB in LOO, WAIC, and LPML across all observed prewarming SOC densities. The Bayesian metrics accounted for AWB's larger model size and increased propensity for overfitting as demonstrated by the consistently higher effective parameter counts associated with AWB (Fig. S8a, b).
Varying prewarming steadystate MIC from MIC1 to MIC8 modestly impaired goodness of fit across the various metrics (Fig. S4). CON's LOO and WAIC values increased respectively from −11.963 and −12.035 at MIC1 to −11.731 and −11.802 at MIC8, while AWB's LOO and WAIC values increased respectively from −8.63 and −9.302 at MIC1 to −8.181 and −8.711 at MIC8 (Table S4c, d). CON did not deteriorate in goodness of fit at a faster rate than AWB with respect to increasing prewarming MIC. Increasing prewarming MIC has the opposite effect on the MICtoSOC ratio compared to increasing prewarming SOC, but both changes worsened goodness of fit across all metrics, indicating that changes to the prewarming MICtoSOC ratio did not produce consistent trends.
Our study develops a quantitative, datadriven framework for model comparison that could be applied across different research questions, ecosystems, and scales. We demonstrated the novel deployment of WAIC and LOO, two more recently developed Bayesian goodnessoffit metrics that estimate model predictive accuracy, to evaluate SBMs using data from longitudinal soil warming experiments. WAIC and LOO improve upon older and more frequently used metrics, such as AIC and DIC, by accounting for model complexity and overfitting of data in a more comprehensive, stable, and accurate fashion. The quantitative agreement between WAIC, LOO, and LPML reinforces the reliability and validity of information criteria and crossvalidation metrics to complement use of frequentist R^{2}.
We constrained the fitting of AWB and CON to biologically reasonable parameter space by fixing prewarming steadystate C pool densities and establishing prior distributions informed by expert judgment (Table S2). We observed that, despite the qualitative difference in the shapes of their mean posterior predictive fit curves, CON and AWB could both potentially account for the soil warming response in the metaanalysis data set. For both models, posterior predictive fit distributions overlapped with the confidence intervals of the data points (Fig. 2). However, with respect to the Bayesian goodnessoffit metrics, CON quantitatively outperformed AWB across all prewarming SOC and MIC densities observed (Figs. 5 and S4) because the Bayesian metrics adjusted for AWB's larger model size and consistently higher effective parameter count (Fig. S8). For both models, lower prewarming SOC densities corresponded to better warming response fits (Fig. 5).
4.1 Model responses to warming over time
After fitting, the response ratio curves of CON and AWB both trended toward the prewarming steadystate response ratio of 1.0 following the soil warming perturbation (Fig. 3). The settling of the curves to the prewarming model steady states aligns with previous literature which demonstrated that the magnitude of CO_{2} flux tends to fall after reaching a postwarming maximum (Crowther et al., 2016; RomeroOlivares et al., 2017). In the metaanalysis data set, this peak is reached immediately at the first data point at t=0.5 years (Fig. 2). CON matched this data pattern in all of our observed simulations in outputting maximum response ratios at the first time point after warming (Figs. S3a, c and S1a, b). AWB was unable to output maximum response ratios at the first time point (Fig. 3b, d) and was therefore penalized in quantitative goodness of fit. Examining AWB's system of equations (Sect. S1b), we surmise that one reason for the later peak was due to the slower growth of MIC in the biologically truncated parameter space that AWB was limited to. MIC is a driving force for the increase in the CO_{2} flux as a numerator term in the AWB flux equation (Sect. S1b, Eq. S11). Unlike MIC biomass in CON (Sect. S1a, Eq. S3), MIC biomass growth in AWB has two loss terms in its differential equation (Sect. S1b, Eq. S8).
This is not to say that CON was clearly superior from a qualitative standpoint. CON's mean posterior predictive curves were not able to match a subsequent local data maximum in the metaanalysis data set at t=3.5 years, a trend which AWB's curves were able to replicate. The mean CON curves also substantially overshoot the data at later time points following t=7.5 years (Figs. 2a and 3a and c, as well as Fig. S1a and b) because of the inability of firstorder linear models such as CON to display oscillatory dynamics (Hale and LaSalle, 1963).
In contrast, AWB displays damped oscillations in its response ratios following warming due to its nonlinear dynamics (Figs. 2 and 3). AWB was able to match the points after t=7.5 years more closely than CON. The presence of respiration oscillations has been observed in longterm warming experiments, such as the one taking place at Harvard Forest (Melillo et al., 2017). It is possible AWB would be quantitatively rewarded in goodnessoffit metrics over CON for its ability to replicate biologically realistic oscillations in larger, sitespecific data sets such as those from Harvard Forest.
4.2 Sensitivity analyses of C pool densities and density ratios
We performed a goodnessoffit sensitivity analysis to check whether the response ratio trends stayed consistent, biologically realistic, and interpretable across a range of prewarming, steadystate soil C densities and pooltopool density ratios. For instance, we imposed constraints to reflect that MICtoSOC density ratios range between 0.01 and 0.04 across various soil types (Anderson and Domsch, 1989; Sparling, 1992). CON and AWB response ratio curves exhibited realistic values and qualitatively consistent shapes across all prewarming SOC and MIC steadystate densities, even at less realistic SOC densities above 100 mg C g^{−1} soil (Fig. 3). There was enough uncertainty in the data that the 95 % posterior predictive intervals for the model output always overlapped with the 95 % confidence intervals of each fitted data point (Fig. 2). In most cases, the posterior mean response ratio curve also fell within the 95 % data confidence interval.
We were unable to initiate our prewarming SOC steadystate density below SOC50 with the priors and MICtoSOC ratios used for AWB. Under SOC50, AWB HMC runs would not reliably run to conclusion and would terminate due to ODE instabilities. Even at SOC50, we saw a reduction in independent and effective samples for certain parameters, namely Ea_{K} and ${E}_{{\mathrm{C}}_{\mathrm{ref}}}$ (Table S5). We did not drop under SOC50 for CON, as we sought to compare AWB and CON at similar MICtoSOC ranges. Our experience underscores the challenge of choosing realistic steadystate soil C densities, density ratios, and prior distributions to obtain valid model comparisons limited to biologically realistic regimes.
The information criteria and crossvalidation fit metrics generally indicated higher relative probability and predictive performance at lower prewarming SOC values for AWB and CON (Fig. 5). The fit results suggest that SOC density of the soil at the sites included in the metaanalysis was likely closer to the lower end of the SOC density ranges examined in our sensitivity analysis. A less pronounced trend toward better fits was observed as prewarming MIC density was decreased while prewarming SOC density was held constant (Fig. S4). No clear relationship was observed between MICtoSOC ratio and goodness of fit in the AWB and CON models.
The worsening IC and CV results at higher SOC densities support the notion that prewarming steadystate SOC densities should not be initialized over SOC100 in AWB and CON when fitting to this metaanalysis data set. Prewarming SOC density was not observed to exceed 50 mg SOC g^{−1} soil at sites included in the metaanalysis, reaching a maximum of 45 mg SOC g^{−1} soil for the top 20 cm in one study with alpine wetland soil (Zhang et al., 2014). The majority of the CO_{2} respired by soil microbes is sourced from surface soil (Fang and Moncrieff, 2005), and it is well documented that SOC densities increase toward the soil surface (Jobbágy and Jackson, 2000). ^{14}C measurements of CO_{2} fluxes suggest that SOC densities representing the source of most heterotrophic respiration range between 40 and 80 mg SOC g^{−1} soil (Trumbore, 2000), so the effective SOC densities associated with soil respiration at some metaanalysis sites may have been in this range.
Overall, the Bayesian metrics from the goodnessoffit sensitivity analysis suggest that CON is superior to AWB at explaining the metaanalysis data set when accounting for model parsimony, particularly when the models are initiated in more realistic ranges of prewarming SOC densities under SOC100. However, we caution against using these results to conclude that CON is a comprehensively superior predictive model over AWB without comparisons involving other longitudinal soil warming data sets. And other data aside, we observe that AWB has a useful advantage over CON conditional on the metaanalysis data set alone: AWB was more tolerant of changes in prewarming conditions, displaying less IC and CV than CON as prewarming SOC is increased (Fig. 5a–c). AWB's compensatory ability stemming from its larger model size could be more quantitatively rewarding in goodnessoffit sensitivity analyses conducted on data assimilations with larger data sets.
For an additional check on the biological realism and plausibility of our simulations, we conducted a sensitivity analysis examining changes in model SOC stocks following warming. The response ratios of postwarming SOC stocks after 12.5 years, evaluated as the ratio of postwarming to prewarming SOC densities, was computed from observed CON and AWB simulations at the posterior parameter means. SOC losses indicated by the response ratios ranged from 8.13 % to 27.1 % across both models (Fig. S3). These results aligned with a recent comprehensive metaanalysis of 143 soil warming studies (Fig. S10). The largest loss of 27.1 %, occurring in CON at SOC50, is sizable, but the metaanalysis included seven studies measuring losses greater than 20 %, with the maximum loss observed at 54.4 % (van Gestel et al., 2018).
Raising prewarming SOC reduced SOC loss after 12.5 years of warming for both models (Fig. S3a). For CON, SOC loss decreased from 27.1 % at SOC50 to 9.2 % at SOC200. For AWB, SOC loss decreased from 17.2 % at SOC50 to 8.13 % at SOC200. Varying prewarming MIC affected the SOC response ratio more substantially for AWB than CON (Fig. S3b). For AWB, SOC loss increased from 11.4 % at MIC1 to 16.3 % at MIC8, while SOC loss decreased from 18.8 % at MIC1 to 17.4 % at MIC8 for CON. The larger effect of increasing MIC on the SOC response ratio in AWB is likely due to MIC influence on SOCtoDOC turnover, which is not a feedback accounted for in the equations of the CON model (Sect. S1a).
The posterior means for the Arrhenius activation energy parameters Ea of CON and AWB returned by the HMC simulations across the observed prewarming C densities (Table S3) differed somewhat from the parameter values used in Allison et al. (2010) and Li et al. (2014), which were in turn tuned based on activation energies estimated in a prior empirical analysis of enzymecatalyzed soil organic matter decomposition processes (TrasarCepeda et al., 2007). In Allison et al. (2010), CON parameters Ea_{S}, Ea_{D}, and Ea_{M} were respectively set at 47, 40, and 40 kJ mol^{−1}, and AWB parameters Ea_{V} and Ea_{VU} were both set at 47 kJ mol^{−1}. The AWB Michaelis–Menten K_{M} terms were not parameterized to have Arrhenius temperature dependence in Allison et al. (2010). In Li et al. (2014), CON parameters Ea_{S}, Ea_{D}, and Ea_{M} were set at 47, 47, and 20 kJ mol^{−1}, and AWB parameters Ea_{V}, Ea_{VU}, Ea_{K}, and Ea_{KU} were set at 47, 47, 30, and 30 kJ mol^{−1}. These values were in line with the activation energies calculated in TrasarCepeda et al. (2007), which ranged from 17.0 to 57.7 kJ mol^{−1}, with the energies corresponding to the decomposition of plant litter and protected organic matter being on the higher end and the energies corresponding to microbial biomass degradation being on the lower.
Our HMC simulations arrived at higher Ea values, with the posterior means of Ea_{S}, Ea_{D}, and Ea_{M} respectively ranging from 51.3 to 77.6, 50.1 to 50.3, and 51.8 to 52.6 kJ mol^{−1} in the prewarming SOCvaried simulations for CON, and the posterior means of Ea_{V}, Ea_{VU}, Ea_{K}, and Ea_{KU} respectively ranging from 58.5 to 74.8, 50.2 to 51.1, 25.8 to 42.4, and 49.0 to 49.8 kJ mol^{−1} for AWB. However, these values are still within the ranges of organic matter decomposition activation energies, which have been empirically estimated to exceed 100 kJ mol^{−1} at their highest in the A horizons of temperate soils (Steinweg et al., 2013), suggesting that the Ea posterior means, aided by prior truncation, effectively remained within biologically realistic space across all observed prewarming C densities. The presence of higher Ea_{S} posterior means also agreed with the empirical trends of higher activation energies for the degradation of SOCrelated organic compounds and lower activation energies for the degradation of material associated with microorganisms.
We found it less useful to compare the posterior means of other fitted parameters including the C pool transfer coefficients, C use efficiency E_{C}, and V_{max} to empirical estimates for biological benchmarking purposes. Unitless parameters like transfer coefficients and E_{C} defy straightforward interpretation, measurement, and estimation from experiments (Bradford and Crowther, 2013). Very different values can be found based on whether substratespecific or substratenonspecific assumptions and methods are used (Geyer et al., 2019; Hagerty et al., 2018). V_{max} parameters are not unitless but display even higher variance than the bounded C transfer and efficiency coefficients. The V_{max} parameter corresponding to a specific enzyme can vary over orders of magnitude when the sensitivity of the enzyme to an interval of temperatures is considered (Nottingham et al., 2016). The process of consolidating experimental substratespecific and substratenonspecific measurements into a single number to correspond to a model V_{max} value introduces further complications and uncertainty, rendering comparisons of potentially drastically different V_{max} values less informative regarding model biological realism.
4.3 HMC parameter space exploration
Truncating prior and posterior parameter distributions proved useful for establishing biological constraints and only modestly deformed posterior densities for AWB and CON. From SOC100 to SOC200, CON and AWB posterior densities showed little or no deformation from typical normal distribution shapes. Moderate posterior density deformation was observed for some parameters in both models at SOC50 and SOC75, namely Ea_{S} for CON and ${E}_{{\mathrm{C}}_{\mathrm{ref}}}$ for AWB (Fig. S11). Even so, most of the other parameter posterior densities still remained undeformed at those SOC values. Thus, prior truncation generally did not prevent posterior means from falling within biologically realistic intervals, suggesting that priors were appropriately informed and chosen.
A small frequency of divergent transitions was detected in the AWB HMC simulations. Divergent transitions can be thought of as algorithm trajectory errors arising during the HMC's exploration of a convoluted region of parameter space; a more thorough description of the theory, computation, and implications of divergent transitions can be found in literature focusing on the Hamiltonian Monte Carlo algorithm (Betancourt, 2016, 2017). The number of divergent transitions generally increased as the prewarming MICtoSOC steadystate ratio was reduced (Fig. S9). Prior truncation and the fixing of select parameters to constrain the prewarming steadystate mass values for biological realism could have played a combined role in generating the Markov chain divergences by hindering the smooth exploration of parameter space. We were unable to eliminate divergent transitions by adjusting HMC parameter proposal step size, suggesting that other methods, such as modification of the HMC algorithm itself or introduction of auxiliary parameters to AWB that reduce correlation between existing model parameters, may be more applicable in reducing divergent transitions in our case (Betancourt and Girolami, 2015). Additionally, the interaction between the ranges of values used for the prior distributions and the limited number of observations in the data set could have contributed to the shaping of geometric inefficiencies (Betancourt, 2017).
It is possible that the instability that prevented consistent solving and HMC exploration of AWB under SOC50 could be traced to the forward Michaelis–Menten formulation of decomposition and uptake kinetics used in the present version of the AWB model (Sect. S1, Eqs. S7, S8). We initialized the system with a small DOC density lower than that of MIC at 0.1 mg C g^{−1} soil. Since DOC was in the denominator of these decomposition and uptake expressions, those expressions could become larger than tolerable for the system in certain parameter regimes.
Some suggestions for the reparameterization of AWB to improve model stability have been proposed that could reduce or even eliminate divergent transitions by facilitating a smoother and steadier parameter space conducive for HMC exploration. One intermediate possibility would be to modify AWB to use reverse Michaelis–Menten kinetics, which would replace the DOC term in the denominators of the decomposition and uptake expressions with the larger MIC term. The use of reverse instead of Michaelis–Menten dynamics has been used to stabilize and constrain other SBMs (Sulman et al., 2014; Wieder et al., 2015b). A more extensive reformulation involves the replacement of Michaelis–Menten expressions with equilibrium chemistry approximation (ECA) kinetics, which would increase the number of denominator terms in decomposition expressions for further stability. ECA equations have been shown to be more consistent in behavior and robust to parameter regime variation than their Michaelis–Menten counterparts, and they thus have been encouraged as a wholesale replacement for Michaelis–Menten formulations (Tang, 2015; Wang and Allison, 2019). These reparameterizations should be implemented and examined in future work that involves sampling and computation of AWB posteriors.
4.4 Outlook and conclusions
Recent SBM comparisons have been unable to demonstrate the superiority of one model over another because the uncertainty boundaries of the data were not sufficient for distinguishing model outcomes (Sulman et al., 2018; Wieder et al., 2014, 2015b, 2018). Similar to these previous studies, our results indicate that more data is needed to constrain and differentiate between model posterior predictive distributions. Conditional on the metaanalysis data set, CON demonstrates superior quantitative goodness of fit over AWB, but we are not confident that the relative model parsimony of CON and other linear firstorder models makes them universally more suitable for predictive use.
Consequently, future SBM comparisons would benefit from additional data collection efforts sourced from longterm ecological research experiments to globally verify the strengths and limitations of linear versus nonlinear SBMs, including CON and AWB, in Earth system modeling. The limited number of longitudinal soil warming studies presents a challenge for facilitating sitespecific model comparisons. We addressed this issue by using metaanalysis data to aggregate warming responses across sites, but this approach does not provide sitespecific parameters. Additional data from ongoing and future field warming studies in the vein of the Harvard Forest and Tropical Responses to Altered Climate experiments that demonstrate more varied flux dynamics over time than the metaanalysis data set will be of critical importance for model testing (Melillo et al., 2017; Wood et al., 2019). Model parameters could also be better constrained through the use of multivariate data sets, for example microbial biomass dynamics in addition to soil respiration.
Our approach can be expanded to compare the predictive accuracies of linear microbialimplicit models to those of recently developed nonlinear microbialexplicit SBMs that are much larger than AWB, such as CORPSE (Sulman et al., 2014) and MIMICS (Wieder et al., 2014). Such comparisons will help broadly determine if inclusion of more detailed microbial dynamics in models offers predictive advantages that can overcome the overfitting burdens associated with an increase in parameter count. With the appropriate data sets, our approach can also be applied to consider the predictive performance of SBMs that describe the cycling of nitrogen (N), phosphorus (P), and other limiting nutrients in addition to C dynamics. Models that represent N and P mineralization have yet to see extensive headtohead statistical benchmarking against Conly models with respect to predictive use (Manzoni and Porporato, 2009). With models growing ever larger in size and specificity, there is a need to verify whether detailed representation of microbial processes and the cycling of limiting nutrients are worth the increase in variable, parameter, and equation counts. After all, “the tendency of more recent models towards more sophisticated (and generally more mathematically complex) approaches is not always paralleled by improved model performance or ability to interpret observed patterns” (Manzoni and Porporato, 2009).
The data assimilation and posterior sampling of complex models in future work comes with computing performance challenges. Markov chain Monte Carlo algorithms are effective for exploring multidimensional parameter space but are limited by temporal and computational expense, particularly when it comes to fitting nonlinear differential equation models (Calderhead et al., 2009; Nemeth and Fearnhead, 2019). Time per Markov chain iteration drastically increases with number of parameters and data points. In fact, the present speed limitations of the family of HMC algorithms make it necessary to use a hybrid approach utilizing Monte Carlo and deep learning algorithms for parameter estimation at a global scale; Monte Carlo fitting is used to constrain parameter estimates at a sitebased scale before those estimates are tuned globally by deep learning using spatial information derived from satellite maps (Tao et al., 2020). However, Monte Carlo algorithms are still the optimal methods for posterior computation (Duan et al., 2018) and are necessary for Bayesian model comparisons conditional on sitebased data. Consequently, recent Monte Carlo algorithm innovations and developments that offer theoretical speed improvements by trading thorough posterior sampling for numerical efficiency have been encouraging and are ripe to be tested in future SBM comparisons involving more complex models and larger data sets. These developments include stochastic gradient Monte Carlo sampling methods, a class of techniques in which a posterior is approximated by fitting to a small subset of data at each iteration rather than estimated through exhaustive sampling (Ma et al., 2015), and Gaussian process acceleration, in which a smooth distribution of likely solutions for a differential equation system is specified and sampled in place of explicitly solving for the state variables during every Markov chain iteration (Calderhead et al., 2009; Dondelinger et al., 2013; Wang and Barber, 2014).
Alongside advances in Monte Carlo algorithms, additional developments in Bayesian crossvalidation and information criteria measures are also available for practical trialing in soil biogeochemical data assimilation. Gelman et al. (2019) have proposed a stable Bayesian counterpart of frequentist R^{2} defined as “the variance of the predicted values divided by the variance of predicted values plus the expected variance of the errors” that allows for more intuitive and direct comparison to R^{2} (Gelman et al., 2019). A Bayesian R^{2} distribution provides a signal about the absolute rather than relative goodness of fit of an associated posterior predictive distribution to the data. Bürkner et al. (2019) have proposed a leavefutureout (LFO) crossvalidation metric which is formulated to estimate relative model predictive accuracy for hypothetical time series data occurring after existing experiment observations. LFO and LOO are computed similarly, and LOO can also be used for time series data, as we demonstrated in this study. However, the algorithmic differences between LFO and LOO make them better suited for different goals. LOO does not inform about the quality of model fits for hypothetical samples collected after final reported measurements and is more appropriate for estimating outofsample model predictive accuracy for hypothetical data samples taken between the interval of observed measurement times (Vehtari et al., 2017).
The development of our formalized, statistically rigorous approach for model comparison and evaluation is a critical step toward the goal of projecting global SOC levels and soil emissions throughout the 21st century. Our initial results indicate promise in continued refinement and expansion of our approach to evaluate the predictive performance of linear and nonlinear SBMs. The future integration of updated Markov chain algorithms and Bayesian predictive accuracy metrics into our framework will expand the ability to efficiently and thoroughly compare differential equation models, even if they vary widely in structure and complexity.
The R scripts, Stan code, and respiration data set used for HMC model fitting along with the original soil respiration metaanalysis data set (RomeroOlivares et al., 2017) are available from the directory located at https://doi.org/10.17605/OSF.IO/7MEY8 (Xie et al., 2020).
The supplement related to this article is available online at: https://doi.org/10.5194/bg1740432020supplement.
SDA and HWX designed the study with assistance from MG. HWX and ALRO performed the data cleaning and analysis. HWX wrote the necessary code for the study with assistance from SDA. SDA and HWX prepared the paper with suggestions from MG.
The authors declare that they have no conflict of interest.
We would like to thank Stan development team members Aki Vehtari (Aalto University), Michael Betancourt (Symplectomorphic, LLC), Bob Carpenter (Flatiron Institute), Ben Bales (Columbia University), Charles Margossian (Columbia University), and Sebastian Weber (Novartis) for their patient help with Stan code implementation and troubleshooting. We would also like to thank both anonymous reviewers for their valuable and constructive comments, which not only aided in the revision of the manuscript but also provided valuable insights to guide future work.
This research has been supported by the National Science Foundation (grant no. DEB1900885), the U.S. Department of Energy Office of Science BERTES program under grant DESC0014374, and the National Institutes of Health T32 Training Program (National Institute of Biomedical Imaging and Bioengineering, grant no. EB009418).
This paper was edited by Trevor Keenan and reviewed by two anonymous referees.
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