Articles | Volume 18, issue 19
https://doi.org/10.5194/bg-18-5351-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-18-5351-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Hydrogen and carbon isotope fractionation factors of aerobic methane oxidation in deep-sea water
Shinsuke Kawagucci
CORRESPONDING AUTHOR
Super-cutting-edge Grand and Advanced Research (SUGAR) Program,
Institute for Extra-cutting-edge Science and Technology Avant-garde Research
(X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC),
Yokosuka 237-0061, Japan
Institute of Geochemistry and Petrology, ETH Zürich,
Zürich 8092, Switzerland
Yohei Matsui
Advanced Science-Technology Research (ASTER) Program, X-star,
JAMSTEC, Yokosuka 237-0061, Japan
Department of Engineering Mechanics and Energy, University of
Tsukuba, Tsukuba 305-0006, Japan
Akiko Makabe
Super-cutting-edge Grand and Advanced Research (SUGAR) Program,
Institute for Extra-cutting-edge Science and Technology Avant-garde Research
(X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC),
Yokosuka 237-0061, Japan
Tatsuhiro Fukuba
Institute for Marine-Earth Exploration and Engineering (MarE3),
JAMSTEC, Yokosuka 237-0061, Japan
Yuji Onishi
Super-cutting-edge Grand and Advanced Research (SUGAR) Program,
Institute for Extra-cutting-edge Science and Technology Avant-garde Research
(X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC),
Yokosuka 237-0061, Japan
The Center for Ecological Research, Kyoto University, Otsu
520-2113, Japan
Takuro Nunoura
Research Institute for Marine Resources Utilization (MRU), JAMSTEC,
Yokosuka 237-0061, Japan
Taichi Yokokawa
CORRESPONDING AUTHOR
Super-cutting-edge Grand and Advanced Research (SUGAR) Program,
Institute for Extra-cutting-edge Science and Technology Avant-garde Research
(X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC),
Yokosuka 237-0061, Japan
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Cited
10 citations as recorded by crossref.
- The stable carbon and hydrogen isotopic composition of microbial fatty acids traces microbial metabolism in soils and peats Y. Zhang et al. https://doi.org/10.1016/j.gca.2023.12.001
- First asteroid gas sample delivered by the Hayabusa2 mission: A treasure box from Ryugu R. Okazaki et al. https://doi.org/10.1126/sciadv.abo7239
- Active Methanotrophs and Their Response to Temperature in Marine Environments: An Experimental Study J. Li et al. https://doi.org/10.3390/jmse9111261
- Deep Subseafloor Biogeochemical Processes and Microbial Populations Potentially Associated with the 2011 Tohoku-oki Earthquake at the Japan Trench Accretionary Wedge (IODP Expedition 343) S. Kawagucci et al. https://doi.org/10.1264/jsme2.ME22108
- Clumped 13C–13C isotopologue signatures of thermogenic ethane K. Taguchi et al. https://doi.org/10.1016/j.gca.2025.02.017
- Clumped isotope measurements reveal aerobic oxidation of methane below the Greenland ice sheet G. Adnew et al. https://doi.org/10.1016/j.gca.2024.11.009
- The evolution of multiply substituted isotopologues of methane during microbial aerobic oxidation J. Li et al. https://doi.org/10.1016/j.gca.2024.06.032
- Reassessing the origin of organic compounds in Mariana forearc serpentinite mud volcanoes: insights from isotopologue and molecular signatures K. Taguchi et al. https://doi.org/10.1186/s40645-026-00806-x
- Tidal and seasonal influence on cold seep activity and methanotroph efficiency in the North Sea T. de Groot et al. https://doi.org/10.1038/s43247-024-01483-8
- Concentrations and isotope ratios of Mo and W in Okinawa Trough hydrothermal fluids: Novel probes for hydrothermal processes in a back-arc basin K. Matsuoka et al. https://doi.org/10.2343/geochemj.GJ25018
10 citations as recorded by crossref.
- The stable carbon and hydrogen isotopic composition of microbial fatty acids traces microbial metabolism in soils and peats Y. Zhang et al. https://doi.org/10.1016/j.gca.2023.12.001
- First asteroid gas sample delivered by the Hayabusa2 mission: A treasure box from Ryugu R. Okazaki et al. https://doi.org/10.1126/sciadv.abo7239
- Active Methanotrophs and Their Response to Temperature in Marine Environments: An Experimental Study J. Li et al. https://doi.org/10.3390/jmse9111261
- Deep Subseafloor Biogeochemical Processes and Microbial Populations Potentially Associated with the 2011 Tohoku-oki Earthquake at the Japan Trench Accretionary Wedge (IODP Expedition 343) S. Kawagucci et al. https://doi.org/10.1264/jsme2.ME22108
- Clumped 13C–13C isotopologue signatures of thermogenic ethane K. Taguchi et al. https://doi.org/10.1016/j.gca.2025.02.017
- Clumped isotope measurements reveal aerobic oxidation of methane below the Greenland ice sheet G. Adnew et al. https://doi.org/10.1016/j.gca.2024.11.009
- The evolution of multiply substituted isotopologues of methane during microbial aerobic oxidation J. Li et al. https://doi.org/10.1016/j.gca.2024.06.032
- Reassessing the origin of organic compounds in Mariana forearc serpentinite mud volcanoes: insights from isotopologue and molecular signatures K. Taguchi et al. https://doi.org/10.1186/s40645-026-00806-x
- Tidal and seasonal influence on cold seep activity and methanotroph efficiency in the North Sea T. de Groot et al. https://doi.org/10.1038/s43247-024-01483-8
- Concentrations and isotope ratios of Mo and W in Okinawa Trough hydrothermal fluids: Novel probes for hydrothermal processes in a back-arc basin K. Matsuoka et al. https://doi.org/10.2343/geochemj.GJ25018
Saved (final revised paper)
Latest update: 18 Sep 2026
Short summary
Hydrogen and carbon isotope ratios of methane as well as the relevant biogeochemical parameters and microbial community compositions in hydrothermal plumes in the Okinawa Trough were observed. We succeeded in simultaneously determining hydrogen and carbon isotope fractionation factors associated with aerobic oxidation of methane in seawater (εH = 49.4 ± 5.0 ‰, εC = 5.2 ± 0.4 ‰) – the former being the first of its kind ever reported.
Hydrogen and carbon isotope ratios of methane as well as the relevant biogeochemical parameters...
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