This study represents a pioneering step in the development of in situ monitoring, reporting, and verification (MRV) frameworks for ocean alkalinity enhancement (OAE), a leading marine carbon dioxide removal strategy. The authors conducted one of the first open-ocean rhodamine tracer release experiments explicitly designed to simulate alkalinity enhancement, demonstrating a scalable and practical approach for tracking the fate of alkalinity additions in dynamic marine environments. By moving beyond theoretical modeling and implementing real world testing, this work establishes a critical methodological foundation for future field trials and offers a replicable strategy for validating carbon removal through OAE.