Articles | Volume 23, issue 20
https://doi.org/10.5194/bg-23-7577-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-23-7577-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Liquid polymer enhances methanogenesis and restructures prokaryotic communities in freshwater sediments
Alexander Feckler
CORRESPONDING AUTHOR
iES Landau, Institute for Environmental Sciences, Department of Natural and Environmental Sciences, RPTU University Kaiserslautern-Landau, Landau, Germany
Eric Bollinger
iES Landau, Institute for Environmental Sciences, Department of Natural and Environmental Sciences, RPTU University Kaiserslautern-Landau, Landau, Germany
Alexandra Unik
iES Landau, Institute for Environmental Sciences, Department of Natural and Environmental Sciences, RPTU University Kaiserslautern-Landau, Landau, Germany
Peter Mueller
iES Landau, Institute for Environmental Sciences, Department of Natural and Environmental Sciences, RPTU University Kaiserslautern-Landau, Landau, Germany
Sabine Filker
Department of Ecology, RPTU University Kaiserslautern-Landau, Kaiserslautern, Germany
Christian Plicht
iES Landau, Institute for Environmental Sciences, Department of Natural and Environmental Sciences, RPTU University Kaiserslautern-Landau, Landau, Germany
Zacharias Steinmetz
iES Landau, Institute for Environmental Sciences, Department of Natural and Environmental Sciences, RPTU University Kaiserslautern-Landau, Landau, Germany
Mirco Bundschuh
iES Landau, Institute for Environmental Sciences, Department of Natural and Environmental Sciences, RPTU University Kaiserslautern-Landau, Landau, Germany
Related authors
No articles found.
Catrina Gore, Peter Mueller, Mark Chapman, Franck Dal Molin, Gerhard Schmiedl, Yvonne Milker, Lisa Benson, and Stefanie Nolte
EGUsphere, https://doi.org/10.5194/egusphere-2026-1559, https://doi.org/10.5194/egusphere-2026-1559, 2026
Short summary
Short summary
Various chemical and physical analyses were performed with mud samples from four restored UK saltmarshes to determine the origin of the organic matter (OM) present. The plants growing on the marsh could have supplied the OM or it could have been washed in by the tides from other ecosystems. In the restored marshes much of the OM came from the latter source and by making comparisons with existing data from natural saltmarshes, it was determined that this was not true of these sites.
Zacharias Steinmetz, Paul Löffler, Silvia Eichhöfer, Jan David, Katherine Muñoz, and Gabriele E. Schaumann
SOIL, 8, 31–47, https://doi.org/10.5194/soil-8-31-2022, https://doi.org/10.5194/soil-8-31-2022, 2022
Short summary
Short summary
To scrutinize the contribution of agricultural plastic covers to plastic pollution, we quantified soil-associated plastic debris (≤ 2 mm) in and around agricultural fields covered with different plastics. PP fleeces and 50 µm thick PE films did not emit significant amounts of plastic debris into soil during their 4-month use. However, thinner and perforated PE foils (40 µm) were associated with elevated PE contents of up to 35 mg kg−1. Their long-term use may thus favor plastic accumulation.
Cited articles
Aitchison, J.: The statistical analysis of compositional data, J. R. Stat. Soc. B, 44, 139–177, https://doi.org/10.1111/j.2517-6161.1982.tb01195.x, 1982.
Al-Harbi, L. M., Kosa, S. A., Baloch, M. K., Bhatti, Q. A., and El-Mossalamy, E.-S. E.-B. H.: Adsorption of polyvinylpyrrolidone over the silica surface: as affected by pretreatment of adsorbent and molar mass of polymer adsorbate, Int. J. Polym. Sci., 2016, 2417292, https://doi.org/10.1155/2016/2417292, 2016.
Anderson, M. J.: Permutation tests for univariate or multivariate analysis of variance and regression, Can. J. Fish. Aquat. Sci., 58, 626–639, https://doi.org/10.1139/f01-004, 2001.
Antić, V. V., Antić, M. P., Kronimus, A., Oing, K., and Schwarzbauer, J.: Quantitative determination of poly(vinylpyrrolidone) by continuous-flow off-line pyrolysis-GC/MS, J. Anal. Appl. Pyrolysis., 90, 93–99, https://doi.org/10.1016/j.jaap.2010.10.011, 2011.
Arp, H. P. H., Kühnel, D., Rummel, C., MacLeod, M., Potthoff, A., Reichelt, S., Rojo-Nieto, E., Schmitt-Jansen, M., Sonnenberg, J., Toorman, E., and Jahnke, A.: Weathering plastics as a planetary boundary threat: exposure, fate, and hazards, Environ. Sci. Technol., 55, 7246–7255, https://doi.org/10.1021/acs.est.1c01512, 2021.
Baek, G., Kim, J., and Lee, C.: A review of the effects of iron compounds on methanogenesis in anaerobic environments, Renew. Sustain. Energy Rev., 113, 109282, https://doi.org/10.1016/j.rser.2019.109282, 2019.
Bhullar, G. S., Edwards, P. J., and Olde Venterink, H.: Variation in the plant-mediated methane transport and its importance for methane emission from intact wetland peat mesocosms, J. Plant Ecol., 6, 298–304, https://doi.org/10.1093/jpe/rts045, 2013.
Blanck, H., Wängberg, S.-Å., and Molander, S.: Pollution-induced community tolerance – a new ecotoxicological tool, Functional Testing of Aquatic Biota for Estimating Hazards of Chemicals, ASTM International, https://doi.org/10.1520/STP26265S, 1988.
Bokulich, N. A., Subramanian, S., Faith, J. J., Gevers, D., Gordon, J. I., Knight, R., Mills, D. A., and Caporaso, J. G.: Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing, Nat. Methods., 10, 57–59, https://doi.org/10.1038/nmeth.2276, 2013.
Bollinger, E., Zubrod, J. P., Lai, F. Y., Ahrens, L., Filker, S., Lorke, A., and Bundschuh, M.: Antibiotics as a silent driver of climate change? A case study investigating methane production in freshwater sediments, Ecotox. Environ. Saf., 228, 113025, https://doi.org/10.1016/j.ecoenv.2021.113025, 2021.
Bollinger, E., Schwilden, P., Lai, F. Y., Schulz, R., Bundschuh, M., and Filker, S.: Higher temperatures exacerbate effects of antibiotics on methanogenesis in freshwater sediment, Commun. Earth Environ., 5, 647, https://doi.org/10.1038/s43247-024-01828-3, 2024.
Bollinger, E., Mayer, J., Lai, F. Y., Schulz, R., Filker, S., and Bundschuh, M.: Adaptation of methanogenic microbial assemblages to antibiotics: The role of resistance genes and taxonomic composition, Environ. Pollut., 383, 126828, https://doi.org/10.1016/j.envpol.2025.126828, 2025.
Brooks, A. L., Wang, S., and Jambeck, J. R.: The Chinese import ban and its impact on global plastic waste trade, Sci. Adv., 4, eaat0131, https://doi.org/10.1126/sciadv.aat0131, 2018.
Brunning, H., Sallach, J. B., Zanchi, V., Price, O., and Boxall, A.: Toward a framework for environmental fate and exposure assessment of polymers, Environ. Toxicol. Chem., 41, 515–540, https://doi.org/10.1002/etc.5272, 2022.
Bürkner, P.-C.: Bayesian item response Modeling in R with brms and Stan, J. Stat. Softw., 100, 1–54, https://doi.org/10.18637/jss.v100.i05, 2021.
Callahan, B. J., McMurdie, P. J., Rosen, M. J., Han, A. W., Johnson, A. J. A., and Holmes, S. P.: DADA2: High-resolution sample inference from Illumina amplicon data, Nat. Methods, 13, 581–583, https://doi.org/10.1038/nmeth.3869, 2016.
Canarini, A., Kaiser, C., Merchant, A., Richter, A., and Wanek, W.: Root exudation of primary metabolites: mechanisms and their roles in plant responses to environmental stimuli, Front. Plant Sci., 10, https://doi.org/10.3389/fpls.2019.00157, 2019.
Canfield, D. E., Kristensen, E., and Thamdrup, B.: Structure and Growth of Microbial Populations, in: Aquatic Geomicrobiology, edited by: Canfield, D. E., Kristensen, E., and Thamdrup, B., Academic Press, vol. 48, 23–64, https://doi.org/10.1016/S0065-2881(05)48002-5, 2005.
Charlesworth, J. C., Beloe, C., Watters, C., and Burns, B. P.: Quorum Sensing in Archaea: Recent Advances and Emerging Directions, in: Biocommunication of Archaea, edited by: Witzany, G., Springer International Publishing, Cham, 119–132, https://doi.org/10.1007/978-3-319-65536-9_8, 2017.
Conrad, R.: Importance of hydrogenotrophic, aceticlastic and methylotrophic methanogenesis for methane production in terrestrial, aquatic and other anoxic environments: A mini review, Pedosphere, 30, 25–39, https://doi.org/10.1016/S1002-0160(18)60052-9, 2020.
Edgar, R. C.: SINTAX: a simple non-Bayesian taxonomy classifier for 16S and ITS sequences, bioRxiv, 074161, https://doi.org/10.1101/074161, 2016.
Feckler, A., Bollinger, E., Unik, A., Mueller, P., Filker, S., Plicht, C., Steinmetz, Z., and Bundschuh, M.: Liquid polymer enhances methanogenesis and restructures prokaryotic communities in freshwater sediments (Version v1), Zenodo [data set and code], https://doi.org/10.5281/zenodo.18859767, 2026.
Filzmoser, P., Maronna, R., and Werner, M.: Outlier identification in high dimensions, Comput. Stat. Data Anal., 52, 1694–1711, https://doi.org/10.1016/j.csda.2007.05.018, 2008.
Geyer, R., Jambeck, J. R., and Law, K. L.: Production, use, and fate of all plastics ever made, Sci. Adv., 3, e1700782, https://doi.org/10.1126/sciadv.1700782, 2017.
Gloor, G. B., Macklaim, J. M., Pawlowsky-Glahn, V., and Egozcue, J. J.: Microbiome datasets are compositional: and this is not optional, Front. Microbiol., 8, https://doi.org/10.3389/fmicb.2017.02224, 2017.
Graves, C., Hinchclidde, T., Johnson, N., Noakes, D., and Williams, S.: Polymers in liquid formulations: a landscape view of the global PLFs market, Royal Society of Chemistry, 2021.
Groh, K. J., Arp, H. P. H., MacLeod, M., and Wang, Z.: Assessing and managing environmental hazards of polymers: historical development, science advances and policy options, Environ. Sci.: Processes Impacts, 25, 10–25, https://doi.org/10.1039/D2EM00386D, 2023.
Guezennec, A. G., Michel, C., Bru, K., Touze, S., Desroche, N., Mnif, I., and Motelica-Heino, M.: Transfer and degradation of polyacrylamide-based flocculants in hydrosystems: a review, Environ. Sci. Pollut. Res., 22, 6390–6406, https://doi.org/10.1007/s11356-014-3556-6, 2015.
Hartmann, N. B., Hüffer, T., Thompson, R. C., Hassellöv, M., Verschoor, A., Daugaard, A. E., Rist, S., Karlsson, T., Brennholt, N., Cole, M., Herrling, M. P., Hess, M. C., Ivleva, N. P., Lusher, A. L., and Wagner, M.: Are we speaking the same language? Recommendations for a definition and categorization framework for plastic debris, Environ. Sci. Technol., 53, 1039–1047, https://doi.org/10.1021/acs.est.8b05297, 2019.
He, Y., Li, Y., Yang, X., Liu, Y., Guo, H., Wang, Y., Zhu, T., Tong, Y., Ni, B.-J., and Liu, Y.: Biodegradable microplastics aggravate greenhouse gas emissions from urban lake sediments more severely than conventional microplastics, Water Res., 266, 122334, https://doi.org/10.1016/j.watres.2024.122334, 2024.
IPCC: Climate Change 2021 – the Physical Science Basis: Working Group I Contribution to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change, 1st Edn., Cambridge University Press, ISBN 9781009157896, 2021.
Julinová, M., Kupec, J., Houser, J., Slavík, R., Marušincová, H., Červeňáková, L., and Klívar, S.: Removal of polyvinylpyrrolidone from wastewater using different methods, Water Environ. Res., 84, 2123–2132, https://doi.org/10.2175/106143012X13373575830999, 2012.
Kuzyakov, Y. and Razavi, B. S.: Rhizosphere size and shape: temporal dynamics and spatial stationarity, Soil Biol. Biochem., 135, 343–360, https://doi.org/10.1016/j.soilbio.2019.05.011, 2019.
Lacroix, E. M., Aeppli, M., Boye, K., Brodie, E., Fendorf, S., Keiluweit, M., Naughton, H. R., Noël, V., and Sihi, D.: Consider the anoxic microsite: acknowledging and appreciating spatiotemporal redox heterogeneity in soils and sediments, ACS Earth Space Chem., 7, 1592–1609, https://doi.org/10.1021/acsearthspacechem.3c00032, 2023.
La Reau, A. J. and Suen, G.: The Ruminococci: key symbionts of the gut ecosystem, J Microbiol., 56, 199–208, https://doi.org/10.1007/s12275-018-8024-4, 2018.
Lide, D. R.: CRC Handbook of Chemistry and Physics, CRC Press, ISBN 0849304857, 2004.
Liu, M., Yan, X., Liu, H., and Yu, W.: An investigation of the interaction between polyvinylpyrrolidone and metal cations, React. Funct. Polym., 44, 55–64, https://doi.org/10.1016/S1381-5148(99)00077-2, 2000.
Liu, X., Li, Y., Tang, J., Wang, D., Guo, S., Liu, Q., and Giesy, J. P.: Nano- and microplastics aided by extracellular polymeric substances facilitate the conjugative transfer of antibiotic resistance genes in bacteria, ACS EST Water, 2, 2528–2537, https://doi.org/10.1021/acsestwater.2c00330, 2022.
Louca, S., Polz, M. F., Mazel, F., Albright, M. B. N., Huber, J. A., O'Connor, M. I., Ackermann, M., Hahn, A. S., Srivastava, D. S., Crowe, S. A., Doebeli, M., and Parfrey, L. W.: Function and functional redundancy in microbial systems, Nat. Ecol. Evol., 2, 936–943, https://doi.org/10.1038/s41559-018-0519-1, 2018.
Luo, Y., Hong, Y., Shen, L., Wu, F., and Lin, X.: Multifunctional role of polyvinylpyrrolidone in pharmaceutical formulations, AAPS PharmSciTech, 22, 34, https://doi.org/10.1208/s12249-020-01909-4, 2021.
Marchandin, H. and Jumas-Bilak, E. R.: The family Veillonellaceae, in: The Prokaryotes, edited by: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K.-H., and Stackebrandt, E., Springer, Berlin, Heidelberg, 433–453, ISBN 978-3-642-30193-3, 2014.
Martin, M.: Cutadapt removes adapter sequences from high-throughput sequencing reads, EMB.net Journal, 17, 10–12, 2011.
McClain, M. E., Boyer, E. W., Dent, C. L., Gergel, S. E., Grimm, N. B., Groffman, P. M., Hart, S. C., Harvey, J. W., Johnston, C. A., Mayorga, E., McDowell, W. H., and Pinay, G.: Biogeochemical hot spots and hot moments at the interface of terrestrial and aquatic ecosystems, Ecosyst., 6, 301–312, 2003.
McDonald, D., Price, M. N., Goodrich, J., Nawrocki, E. P., DeSantis, T. Z., Probst, A., Andersen, G. L., Knight, R., and Hugenholtz, P.: An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea, ISME J., 6, 610–618, https://doi.org/10.1038/ismej.2011.139, 2012.
Megonigal, J. P., Hines, M. E., and Visscher, P. T.: 8.08 – Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes, in: Treatise on Geochemistry, edited by: Holland, H. D. and Turekian, K. K., Pergamon, Oxford, 317–424, https://doi.org/10.1016/B0-08-043751-6/08132-9, 2003.
Middelanis, T., Looschelders, D., Mueller, P., and Knorr, K.-H.: Potential of biochar to mitigate methane production in paddy soils – application of a new incubation and modelling approach, Biogeochemistry, 168, 6, https://doi.org/10.1007/s10533-024-01200-9, 2024.
Mosbæk, F., Kjeldal, H., Mulat, D. G., Albertsen, M., Ward, A. J., Feilberg, A., and Nielsen, J. L.: Identification of syntrophic acetate-oxidizing bacteria in anaerobic digesters by combined protein-based stable isotope probing and metagenomics, ISME J., 10, 2405–2418, https://doi.org/10.1038/ismej.2016.39, 2016.
Mueller, P., Mozdzer, T. J., Langley, J. A., Aoki, L. R., Noyce, G. L., and Megonigal, J. P.: Plant species determine tidal wetland methane response to sea level rise, Nat. Commun., 11, 5154, https://doi.org/10.1038/s41467-020-18763-4, 2020.
National Institute of Standards and Technology: NIST Inorganic Crystal Structure Database, NIST Standard Reference Database Number 3, https://doi.org/10.18434/M32147, 2009.
Oksanen, J., Simpson, G. L., Blanchet, F. G., Kindt, R., Legendre, P., Minchin, P. R., O'Hara, R. B., Solymos, P., Stevens, M. H. H., Szoecs, E., Wagner, H., Barbour, M., Bedward, M., Bolker, B., Borcard, D., Carvalho, G., Chirico, M., De Caeres, M., Durand, S., Antoniazi Evangelista, H. B., FitzJohn, R., Friendly, M., Furneaux, B., Hannigan, G., Hill, M. O., Lahti, L., McGlinn, D., Ouellette, M.-H., Ribeiro Cunha, E., Smith, T., Stier, A., Ter Braak, C. J. F., and Weedon, J.: vegan: Community Ecology Package, R package version 2.6-6.1, https://CRAN.R-project.org/package=vegan (last access: 20 August 2026), 2022.
R Core Team: R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing, Vienna, Austria, https://www.R-project.org/ (last access: 20 August 2026), 2025.
Rabaey, J. S., Holgerson, M. A., Richardson, D. C., Andersen, M. R., Bansal, S., Bortolotti, L. E., Cotner, J. B., Hornbach, D. J., Martinsen, K. T., Moody, E. K., and Schloegel, O. F.: Freshwater biogeochemical hotspots: high primary production and ecosystem respiration in shallow waterbodies, Geophys. Res. Lett., 51, e2023GL106689, https://doi.org/10.1029/2023GL106689, 2024.
Rognes, T., Flouri, T., Nichols, B., Quince, C., and Mahé, F.: VSEARCH: a versatile open source tool for metagenomics, PeerJ, https://doi.org/10.7717/peerj.2584, 2016.
Saunois, M., Martinez, A., Poulter, B., Zhang, Z., Raymond, P. A., Regnier, P., Canadell, J. G., Jackson, R. B., Patra, P. K., Bousquet, P., Ciais, P., Dlugokencky, E. J., Lan, X., Allen, G. H., Bastviken, D., Beerling, D. J., Belikov, D. A., Blake, D. R., Castaldi, S., Crippa, M., Deemer, B. R., Dennison, F., Etiope, G., Gedney, N., Höglund-Isaksson, L., Holgerson, M. A., Hopcroft, P. O., Hugelius, G., Ito, A., Jain, A. K., Janardanan, R., Johnson, M. S., Kleinen, T., Krummel, P. B., Lauerwald, R., Li, T., Liu, X., McDonald, K. C., Melton, J. R., Mühle, J., Müller, J., Murguia-Flores, F., Niwa, Y., Noce, S., Pan, S., Parker, R. J., Peng, C., Ramonet, M., Riley, W. J., Rocher-Ros, G., Rosentreter, J. A., Sasakawa, M., Segers, A., Smith, S. J., Stanley, E. H., Thanwerdas, J., Tian, H., Tsuruta, A., Tubiello, F. N., Weber, T. S., van der Werf, G. R., Worthy, D. E. J., Xi, Y., Yoshida, Y., Zhang, W., Zheng, B., Zhu, Q., Zhu, Q., and Zhuang, Q.: Global Methane Budget 2000–2020, Earth Syst. Sci. Data, 17, 1873–1958, https://doi.org/10.5194/essd-17-1873-2025, 2025.
Shi, T., Peng, C., Lu, L., Yang, Z., Wu, Y., Wang, Z., and Kappler, A.: Response of Fe(III)-reducing kinetics, microbial community structure and Fe(III)-related functional genes to Fe(III)-organic matter complexes and ferrihydrite in lake sediment, Biogeochemistry, 167, 1553–1565, https://doi.org/10.1007/s10533-024-01186-4, 2024.
Siebel, D., Scharfer, P., and Schabel, W.: Determination of concentration-dependent diffusion coefficients in polymer–solvent systems: analysis of concentration profiles measured by raman spectroscopy during single drying experiments excluding boundary conditions and phase equilibrium, Macromolecules, 48, 8608–8614, https://doi.org/10.1021/acs.macromol.5b02144, 2015.
Sobieraj, M. and Boone, D. R.: Syntrophomonadaceae, in: The Prokaryotes: Volume 4: Bacteria: Firmicutes, Cyanobacteria, edited by: Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K.-H., and Stackebrandt, E., Springer US, New York, NY, 1041–1049, https://doi.org/10.1007/0-387-30744-3_37, 2006.
Sousa, D. Z., Smidt, H., Alves, M. M., and Stams, A. J. M.: Ecophysiology of syntrophic communities that degrade saturated and unsaturated long-chain fatty acids, FEMS Microbiol. Ecol., 68, 257–272, https://doi.org/10.1111/j.1574-6941.2009.00680.x, 2009.
Stan Development Team: RStan: the R interface to Stan. R package version 2.32.3, http://mc-stan.org/, 2023.
Steinmetz, Z., Kintzi, A., Muñoz, K., and Schaumann, G. E.: A simple method for the selective quantification of polyethylene, polypropylene, and polystyrene plastic debris in soil by pyrolysis-gas chromatography/mass spectrometry, J. Anal. Appl. Pyrolysis, 147, 104803, https://doi.org/10.1016/j.jaap.2020.104803, 2020.
Steinmetz, Z., Plicht, C., Buchmann, C., Knott, M., Meyer, M., Müller-Schüssele, S., Strieth, D., Prosenc, M. H., Steinmetz, H., Jungkunst, H. F., Thiel, W. R., and Bundschuh, M.: Plastic problem solved? Environmental implications of synthetic hydrophilic polymers across ecosystem boundaries, Trends Anal. Chem., 181, 118000, https://doi.org/10.1016/j.trac.2024.118000, 2024.
Walker, T. R. and Fequet, L.: Current trends of unsustainable plastic production and micro(nano)plastic pollution, Trends Anal. Chem., 160, 116984, https://doi.org/10.1016/j.trac.2023.116984, 2023.
Walters, W., Hyde Embriette, R., Berg-Lyons, D., Ackermann, G., Humphrey, G., Parada, A., Gilbert, J. A., Jansson, J. K., Caporaso, J. G., Fuhrman, J. A., Apprill, A., and Knight, R.: Improved bacterial 16S rRNA gene (V4 and V4-5) and fungal internal transcribed spacer marker gene primers for microbial community surveys, mSystems, 1, https://doi.org/10.1128/msystems.00009-15, 2015.
Wang, D., Zheng, Y., Deng, Q., and Liu, X.: Water-soluble synthetic polymers: their environmental emission relevant usage, transport and transformation, persistence, and toxicity, Environ. Sci. Technol., 57, 6387–6402, https://doi.org/10.1021/acs.est.2c09178, 2023.
Wang, X., Xu, Y., Ou, Q., Chen, W., van der Meer, W., and Liu, G.: Adsorption characteristics and mechanisms of water-soluble polymers (PVP and PEG) on kaolin and montmorillonite minerals, J. Haz. Mat., 466, 133592, https://doi.org/10.1016/j.jhazmat.2024.133592, 2024.
Wang, Z. P., DeLaune, R. D., Patrick Jr., W. H., and Masscheleyn, P. H.: Soil redox and pH effects on methane production in a flooded rice soil, Soil Sci. Soc. Am. J., 57, 382–385, https://doi.org/10.2136/sssaj1993.03615995005700020016x, 1993.
Watanabe, A. and Kuwatsuka, S.: Chemical characteristics of soil fulvic acids fractionated using polyvinylpyrrolidone (PVP), Soil Sc. Plant Nutr., 38, 31–41, https://doi.org/10.1080/00380768.1992.10416949, 1992.
Waters, C. M. and Bassler, B. L.: QUORUM SENSING: Cell-to-cell dommunication in bacteria, Ann. Rev. Cell Dev. Biol., 21, 319–346, https://doi.org/10.1146/annurev.cellbio.21.012704.131001, 2005.
Weiss, R. F.: Carbon dioxide in water and seawater: the solubility of a non-ideal gas, Mar. Chem., 2, 203–215, https://doi.org/10.1016/0304-4203(74)90015-2, 1974.
Wenig, P. and Odermatt, J.: OpenChrom: a cross-platform open source software for the mass spectrometric analysis of chromatographic data, BMC Bioinform., 11, 405, https://doi.org/10.1186/1471-2105-11-405, 2010.
Westerholm, M., Calusinska, M., and Dolfing, J.: Syntrophic propionate-oxidizing bacteria in methanogenic systems, FEMS Microbiol. Rev., 46, fuab057, https://doi.org/10.1093/femsre/fuab057, 2022.
Wilkinson, J., Bors, C., Burgis, F., Lorke, A., and Bodmer, P.: Measuring CO2 and CH4 with a portable gas analyzer: closed-loop operation, optimization and assessment, PloS One, 14, e0206080, https://doi.org/10.1371/journal.pone.0193973, 2018.
Xue, X., Pasparakis, G., Halliday, N., Winzer, K., Howdle, S. M., Cramphorn, C. J., Cameron, N. R., Gardner, P. M., Davis, B. G., Fernández-Trillo, F., and Alexander, C.: Synthetic polymers for simultaneous bacterial sequestration and quorum sense interference, Angew. Chem. Int. Ed., 50, 9852–9856, https://doi.org/10.1002/anie.201103130, 2011.
Zhang, D., Chen, Y., Zhao, Y., and Zhu, X.: New sludge pretreatment method to improve methane production in waste activated sludge digestion, Environ. Sci. Technol., 44, 4802–4808, https://doi.org/10.1021/es1000209, 2010.
Zhang, W., Liu, X., Liu, L., Lu, H., Wang, L., and Tang, J.: Effects of microplastics on greenhouse gas emissions and microbial communities in sediment of freshwater systems, J. Haz. Mat., 435, 129030, https://doi.org/10.1016/j.jhazmat.2022.129030, 2022.
Zhou, G., Shi, Q.-S., Huang, X.-M., and Xie, X.-B.: The three bacterial lines of defense against antimicrobial agents, Int. J. Mol. Sci., 16, 21711–21733, https://doi.org/10.3390/ijms160921711, 2015.
Short summary
Synthetic water-soluble plastics are widely used, yet their effects on lake and river sediments remain unclear. We investigated polyvinylpyrrolidone by exposing freshwater sediments and monitoring methane production and microbial communities for 56 d under oxygen-free conditions. The plastic caused earlier and greater methane release and shifted bacterial populations, particularly those feeding methane producers.
Synthetic water-soluble plastics are widely used, yet their effects on lake and river sediments...
Altmetrics
Final-revised paper
Preprint